JPS5948064A - Preparation of extract of sesame - Google Patents

Abstract

PURPOSE:To obtain an extract having extremely effective effect on prevention of change of properties by a very simple method, by extracting ground seed of sesame by a proper combination of extraction operations selecting ethanol-containing aqueous solvents. CONSTITUTION:100pts.wt. ground sesame seed such as defatted sesame seed, pressed sesame seed, etc. is extracted with >=250pts.wt. water alone or mixed solvent of ethanol and water containing >=5wt% water at room temperature - 50 deg.C. Unstable materials are removed from this extract by operations such as filtration, centrifugation, etc. to give an extracted solution, which is concentrated or dried. The concentration or drying is carried out until the total amount becomes <=40pts.wt. and the water content in the remaining solvent is >=50wt%. The condensate or dried material is extracted again with ethanol alone or a mixed solvent of ethanol and water. In this case, a weight ratio of ethanol and water is (100:0)-(60:40), and the total amount is <=100pts.wt. Consequently, an extract of sesame capable of preventing oxidation of foods, change of properties, etc. extremely effectively can be obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing a sesame extract, and more particularly to a method for producing a sesame extract that can extremely effectively prevent deterioration of foods due to oxidative deterioration and discoloration.

It is well known that edible fats and oils and processed foods containing them lose their freshness and deteriorate due to autooxidation of the fats and oils during processing and storage. Therefore, these fats and oil-containing processed foods, such as animal and vegetable oils and fats such as soybean oil, sesame oil, rapeseed oil, safflower oil, coconut oil, chicken oil, lard or fish oil, dressings, mayonnaise, shortening, cheese, In oil-containing processed foods such as butter, marcarin, ham, sausage, instant Chinese noodles, and fried sweets, some kind of measure to prevent oxidation is usually taken. In addition, discoloration of pigments and colorants such as carotenes naturally contained or as additives is observed in natural or processed beverages such as juices and carbonated drinks, which is facilitated by sunlight and artificial light sources. Similarly, it is necessary to take some measures to prevent color fading.

Conventionally, various antioxidants have been widely used as food additives in order to maintain the quality of foods against such deterioration due to oxidative deterioration, discoloration, etc. of foods.

However, there are a wide variety of food additives of this kind, both natural and artificial, but natural products, which are highly safe, do not have sufficient performance in preventing deterioration, and recently there has been an emphasis on reconsidering the safety of artificial islands. Therefore, in reality, there is a strong desire for a material that is safer and has an excellent ability to prevent deterioration due to oxidative deterioration and discoloration.

In view of these circumstances, the present inventors focused on the various components contained in sesame seeds and conducted intensive research on methods for effectively collecting them, in order to obtain highly safe and excellent deterioration-preventing components. As a result, by selecting a specific solvent and appropriately combining extraction operations from crushed sesame seeds such as defatted sesame seeds or pressed sesame seeds, a very simple method has an extremely effective deterioration prevention effect. It was discovered that an extract having the following properties can be obtained, and the present invention was completed.

That is, the present invention provides a method for producing a sesame extract that is effective as an agent for preventing deterioration of foods.
Extract by adding at least 250 parts by weight of water alone or a mixed solvent of ethanol and water containing 5 parts by weight or more of water to the parts by weight, remove insoluble matter, and concentrate or dry the resulting extract. Adjust the extract so that the amount is 40 parts by weight or less and the water content in the residual solvent is 50% by weight or more, then add ethanol alone or a mixed solvent of ethanol and water so that the weight ratio of ethanol:water is in the range of 100:0 to 6040. The present invention relates to a method for producing a sesame extract that can extremely effectively prevent deterioration of foods due to oxidative deterioration, discoloration, etc., which is characterized in that the sesame extract is extracted again in a range where the total amount is 100 parts by weight or less.

The sesame extract in the present invention is obtained by extracting sesame seeds twice using water or ethanol alone, or a mixed solvent consisting of ethanol and water, as described above, and the oil-soluble substances may be extracted depending on the characteristics of the solvent used. Contains almost no In other words, the extract does not contain tocopherols, sezamol, etc., which are conventionally known as natural antioxidant substances contained in oils and fats, to the extent that they can exhibit substantial effects, and are not suitable for foods. However, unlike the above-mentioned natural antioxidant substances, the component that has a substantial effect of preventing deterioration is a water- or ethanol-soluble component.

Hereinafter, the configuration of the present invention will be explained in more detail.

As the raw material used in the present invention, defatted sesame seeds are preferable because they have a low content of oils and fats that are unnecessary for the resulting sesame extract. There are various methods of oil extraction that separate sesame seeds into sesame oil and defatted sesame seeds. Usually, the oil is extracted mechanically under high pressure from sesame seeds as they are or after roasting them, but in some cases defatted sesame seeds are extracted after oil extraction. Seeds may also be extracted with hexane. The raw material used in the present invention may be any defatted sesame seeds, regardless of whether or not they have been roasted or extracted with a solvent. In addition, sesame seeds before defatting can also be used, but in this case, pressed seeds are used for better extraction effectiveness.

In the present invention, these raw materials are used for two extractions, one before and one after. - In the second extraction, for 100 parts by weight of the raw material,
Ethanol containing water alone or 5 parts by weight or more of water.
This is carried out by adding 250 parts by weight or more of a water mixed solvent. As the water content of the extraction solvent increases, the raw materials swell and the yield of the extract decreases, so it is better to increase the amount of solvent used, and when using a solvent containing water of 50% by weight or more. 400 for
It is preferable to extract with a solvent in an amount of 1 part by weight or more. However, 10
Even if a solvent of 00 parts by weight or more is used, the extraction effect will not be improved so much, and it will be disadvantageous in terms of subsequent concentration or drying, making it impractical.On the other hand, when using a solvent with a high ethanol content, The amount of the solvent may be small within the above-mentioned condition range, but if a solvent with an ethanol content exceeding 95% by weight is used, the residual oil in the raw material will be mainly extracted, and the antioxidant performance and other deterioration prevention performance will be reduced. Therefore, it is impossible to obtain an extract with high specific activity, and the yield of active ingredients other than oil becomes extremely low. - For the second extraction, there are no particular limitations on anything other than the solvent composition and the amount used for the raw materials. For example, the optimal extraction time varies greatly depending on the equipment and other conditions, so it is selected as appropriate, but the extraction temperature is In order to prevent deterioration of the product, it is preferable to carry out the process at a temperature ranging from room temperature to 5oC. If the extraction conditions are changed within the above-mentioned range, the contents of the -th extract will differ somewhat depending on the extraction conditions, but by adding the second extraction operation described below to the extract, the contents of the final extract will change. There is no problem because the fluctuation can be suppressed.

The slurry obtained from the first extraction is separated into an extract and an insoluble component by filtration, centrifugation, etc., and then the extract is concentrated or dried. There are no particular limitations on the concentration or drying method, but since the extract contains sugars and small amounts of proteins, it is preferable to carry out the concentration or drying at as low a temperature as possible to prevent deterioration and coloration of these substances. At this time, the concentration was such that some of the solvent remained within the range that did not interfere with the next second extraction. However, in view of the final extraction effect, it is necessary to continue until the total amount is 40 parts by weight or less and the water content in the residual solvent is 50% by weight or more. When the residual solvent in the concentrate has a water content of approximately 100% by weight, it is preferable to concentrate the total amount to 30 parts by weight or less in terms of the final extraction effect.

A second extraction is performed on the concentrate or dried product thus obtained. That is, ethanol alone or a mixed solvent of ethanol and water is added to the concentrate or dried material, and the solvent composition during extraction is adjusted to a weight ratio of ethanol to water of 100:O to 60.
40 and the total amount is 100 parts by weight or less. The content ratio of ethanol in the solvent composition is 6
If the ratio is less than 0, or if the total amount is more than 100 parts by weight, inactive components such as antioxidant properties cannot be effectively removed. However, in terms of the effectiveness of extracting the active ingredients, it is practical to extract the total amount at 15 parts by weight or more. In the second extraction, the extraction conditions are the same as those for the first extraction, except for the solvent composition and amount used.

After the second extraction, the insoluble matter is separated in the same way as the second extraction, and the resulting extract can be directly concentrated and dried, or the extract can be decolorized with activated carbon and concentrated or dried. In either case, the effectiveness of the sesame extract produced according to the present invention is not impaired.

In the present invention, when using raw materials containing a large amount of fats and oils, it is desirable to remove the fats and oils by methods such as solvent extraction, separation, and adsorption during or after the second or second extraction. . Further, various other operations may be incorporated into the operations described above as long as the characteristics of the basic operations of the present invention are not impaired.

The present invention is a simple method using ethanol and/or water as explained above, and the sesame extract produced by this method can be used alone, as a solution, or in combination with other components. It exhibits excellent anti-oxidation properties and other deterioration prevention properties.

Finally, in order to make the present invention more specific, Examples and the effects of the obtained sesame extract will be shown.

・Example 1 1 kg of defatted sesame seeds (residual oil content 8.8%) obtained by extracting oil from Chinese sesame seeds using an oil pressing machine was taken into a 10/4-bottle flask (with a stirrer), and transferred to n- to remove the residual oil. Kisan 4k
After stirring and extracting at room temperature for 4 hours, the slurry was filtered through Nunoche to separate the solid content, and dried under ventilation at room temperature. The entire amount of these defatted sesame seeds was taken into a 10/4 cup flask (with a stirrer), and extracted with 80% (w/w) x 4.5 kg of ethanol at room temperature for 10 hours with stirring.
The slurry was filtered through Nusoche to obtain 8.92 kg of extract. Pour 1 kg of this extract into an evaporator and heat at 50°C.
The ethanol content was removed and lyophilized to obtain 14 g of an extract (this will be referred to as extract A). Separately, remaining extract 29
2 kg was taken into a 51-piece four-loaf flask (with a stirrer),
Concentrate at 40 to 50°C under reduced pressure to obtain 63 g of a concentrated solution with a solid content of 42.3% (water content in the residual solvent is approximately 100%), and add 100 g of 99% (W/W) ethanol to this again at room temperature. The mixture was gradually added while stirring, and the mixture was extracted with stirring for 1 hour. Next, 107 g of the extract was obtained by removing the insoluble content using a Nutsche, and removing the ethanol content using an evaporator at 50°C.
Freeze-drying yielded 12 g of the extract according to the invention (designated as extract B).

The extract A obtained here is brown, heterogeneous solid, and has no water content.
．． 8%, oil content 2.7%, total sugar content (glucose standard) 63
．． 1, the nitrogen content was 111% (69% in terms of tannochlorite). The strained extract B is a yellowish brown solid with 0 water content.
4. Nitrogen content: 0.96% (protein equivalent: 6.0%)
), which was dispersed in water and sparingly soluble in fats and oils. When the content of tocopherol and cezamol was measured for extract B, the total +- copherol was 9 ppm.
Cezamol was not detected.

・Example 2 1 kg of defatted sesame seeds (residual oil content 8.8%) obtained by extracting oil from Chinese sesame seeds using an oil press machine was placed in four 10 flasks (with a stirrer), 75 kg of distilled water was added, and the mixture was heated to room temperature. After stirring and extraction for 10 hours, the slurry was filtered through a Nunoche to obtain 4.52 kg of extract. This extract was freeze-dried to obtain 705 g of an extract (designated as extract C). Next, 500s+ of 30g of this extract C was placed in four Lof flasks (
(with a stirrer), dissolve (partially insoluble) in 30 g of water, and add 99% (w/w) ethanol 300 to 99% (w/w) while stirring at room temperature.
g was gradually added, stirred and extracted for 30 minutes, and filtered through Nunoche to remove insoluble matter. The ethanol content of the obtained extract was removed using an evaporator at 50° C., and the extract was freeze-dried to obtain 6.4 g of the extract according to the present invention (this is referred to as the extract).

Here, Tokuda extract C was a brown solid with a water content of 1.1% and a nitrogen content of 8.74% (28.4% in terms of protein). The extract was still a yellowish brown solid with a water content of 0.8%, a nitrogen content of 122% (7.6% in terms of protein), and water dispersibility.

・Example 3 1 kg of defatted sesame seeds (residual oil content 9.0%) obtained by extracting oil from Colombian sesame seeds using an oil pressing machine was placed in a 10/4 flask (with a stirrer), and 92% (W/W) ethanol was added. After adding 4.0 kg and stirring for extraction at room temperature for 10 hours, the slurry was filtered through Nunoche to obtain 311hg of extract. The total amount of this extract was taken into a four-loaf flask (with a stirrer)9, and concentrated under reduced pressure at 40-50°C to reduce the total amount to 2.
27 g, and transferred to a separatory funnel to separate and remove the oil, yielding 188 g of a concentrated solution with a solid content of 11.0% (water content in the residual solvent: 651%). Add 400 g of 99φ (W/w) ethanol gradually while stirring at room temperature, continue stirring and extracting for 30 minutes, separate the insoluble matter with a Nunoche, and add 521 g of the extract. It's a good deal.

10 g of activated carbon was added to the entire extract, stirred at room temperature for 30 minutes, filtered through Nusoche, and the resulting p solution was heated at 50°C to remove the ethanol content with an evaporator.
After lyophilization, 10.6 g of the extract according to the present invention (designated as extract E) was obtained.

Tokuda extract E was a yellowish brown solid with a water content of 0.4% and a nitrogen content of 0.24% (15 parts in terms of protein).

・Comparative Example 1 1 kg of defatted sesame seeds (residual oil content 9.0%) obtained by extracting oil from Colombian sesame seeds using an oil presser was taken into a 10/4 flask (with a stirrer), and 99% (W/W) was obtained.
After adding 4.0 kg of ethanol and stirring for extraction at room temperature for 10 hours, the mixture was filtered through Nunoche to obtain 3.23 kg of an extract. The entire amount of this extract was desolvated using an evaporator at 40 to 50° C. under reduced pressure to obtain 102 g of a heterogeneous oily extract. Next, the entire amount of this extract was mixed with 400% of 1-hexane (
1 and 300 g of 80% (w/w) ethanol. When the n-hexane layer was dried, 98 g of a yellow-brown oil was obtained, which was analyzed to be triglyceride (containing 0.018% of γ-tocopherol). On the other hand, from the ethanol/water layer, 2.117 brown semi-solid substances (this was referred to as Extract F) were obtained. This extract F had a water content of 0.23 and an acid value of 281.

・Performance experiment 1 Mayonnaise with the following blending ratio was manufactured, and extracts A-F, dl-α-tocopherol,
Commercially available natural antioxidant (manufactured by Lion Macorminok Co., Ltd., 5P-
10) was added and stored at 35°C. After prescribed oral administration,
The fats and oils in the mayonnaise are extracted with ethyl ether and the peroxide value (milliequivalents/41) is measured using a conventional method, which is used as an antioxidant index for the fats and oils in the mayonnaise.

Highly refined sesame oil” 100C1yZ food
vinegar 80g egg yolk 100g salt
15g (*1 = Purified through a column packed with basic alumina in order to remove the antioxidant components, pigments, etc. contained) The results are shown in Table 1.

The results shown in Table 1 also show that both Extract B and DXE according to the present invention have better antioxidant properties than the others.

・Performance experiment-2 Water-soluble β-carotene powder (manufactured by Sankyosha) was dissolved and diluted in water.
An aqueous solution containing 6 ppm of β-carotene was prepared.

Extract A-FXdl-α”□゛′′-tocopherol natural antioxidant (manufactured by Lion Macorminok Co., Ltd., 5
P-100) respectively, extract A and extract C were 1%
Prepare an aqueous solution and a 1% ethanol solution for the other, and add each to the second
The amounts listed in the table were added to the β-carotene solution and mixed thoroughly. 50 ml of this sample was transferred to a 70M glass cylinder, left sealed, and the number of days until the orange color was completely no longer recognized under indoor diffused light was measured, and this was used as an index of antifading property for each β-carotene.

The results are shown in Table 2.

From the results shown in Table 2, it can be seen that both extracts B and DlE according to the present invention have better anti-fading properties than the others.

Patent Applicant Takemoto Yushi Co., Ltd. Agent Patent Attorney Hiroshi Iriyama Director of the Patent Office Kazuo Wakasugi 20 Title of Invention Method for Producing Sesame Extract 3, Relationship to the Amendment Case Patent Applicant Address Aichi Prefecture 2-5-4, Minato-cho, Gamagori City, Agent - Address: 5-21-16-5 Sakae, Naka-ku, Nagoya, Aichi Prefecture
゜Date of amendment order (voluntary) 6. Subject of amendment Scope of claims and detailed description of the invention column 7 of the specification Contents of amendment Announcement of attached sheet, Contents of amendment (1) Description No. 5 Correct "...water or ethanol soluble" in the third line of the page to "...water or ethanol soluble or dispersible".

(2) “Extract 102g...” on page 14, line 3 of the specification.
" is corrected to [Extract 62f...].

(3) On page 14, line 6 of the specification: “...oily substance 981.
``...'' 1...58f of oily substance! "..." he corrected himself.

2. Claims 1 100 parts by weight of crushed sesame seeds are extracted with water alone or 250 parts by weight or more of an ethanol-water mixed solvent containing 5% by weight or more of water to remove insoluble matter. After that, the obtained extract is concentrated or dried to obtain 4
0 parts by weight or less and the water content in the residual solvent is 50 parts by weight or more, then ethanol alone or a mixed solvent of ethanol and water is added so that the weight ratio of ethanol to water is],
OO: A method for producing a sesame extract, characterized by using a solvent in the range of O to 60:40 and extracting again in a range where the total amount is 100 parts by weight or less.

Claims (1)

[Claims] 1. Extract 100 parts by weight of crushed sesame seeds by adding 250 parts by weight or more of water alone or a mixed solvent of ethanol and water containing 5 weight or more of water, remove insoluble matter, and extract the resulting extract. Concentrate or dry the extract to 40%
The water content in the residual solvent should be 50 parts by weight or less, and then ethanol alone or a mixed solvent of ethanol and water should be added to adjust the weight ratio of ethanol to water to Zoo.
: A method for producing a sesame extract, characterized by using a solvent in the range of 0 to 60°40 and extracting again in a range where the total amount is 100 parts by weight or less.