JPS5942962U

JPS5942962U - immobilized enzyme membrane

Info

Publication number: JPS5942962U
Application number: JP13972482U
Authority: JP
Inventors: 滝沢　耕一; 聡中嶋; 白川　義貴; 真人荒井
Original assignee: オムロン株式会社
Priority date: 1982-09-13
Filing date: 1982-09-13
Publication date: 1984-03-21

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Brief explanation of drawings]

第１図はこの考案の一実施例を示す断面図解図である。第２図は第１図実施例を利用した酵素電極の一例を示す
断面図解図である。第３図はこの考案の他の実施例を示
す断面図解図である。第４図は第３図実施例を利用した
酵素電極の一例を示す断面図解図である。第５図はこの
考案が有利に実施され得る具体的な測定装置のための酵
素電極の好ましい例を示す断面図解図である。第６図は
第５図において膜ホルダを電極側から見た平面的図解図
である。第７図はこめ考案の固定化酵素膜を装着した酵
素電極が利用され得る測定装置のシ　　　□ステムダイ
ヤグラムある。第８図はこの考案に従った固定化酵素膜
の製造方法を工程順次に示す図解図である。図において、１は固定化酵素膜、１１は高分子　・薄膜
、１２は酵素固定化膜を示す。FIG. 1 is an illustrative cross-sectional view showing one embodiment of this invention. FIG. 2 is an illustrative cross-sectional view showing an example of an enzyme electrode using the embodiment shown in FIG. FIG. 3 is an illustrative cross-sectional view showing another embodiment of this invention. FIG. 4 is an illustrative cross-sectional view showing an example of an enzyme electrode using the embodiment shown in FIG. FIG. 5 is an illustrative cross-sectional view showing a preferred example of an enzyme electrode for a specific measuring device in which this invention can be advantageously implemented. FIG. 6 is an illustrative plan view of the membrane holder in FIG. 5, viewed from the electrode side. Figure 7 is a system diagram of a measuring device in which the enzyme electrode equipped with the immobilized enzyme membrane devised by Kome can be used. FIG. 8 is an illustrative diagram showing the method for producing an immobilized enzyme membrane according to this invention step by step. In the figure, 1 is an immobilized enzyme membrane, 11 is a polymer/thin membrane, and 12 is an enzyme-immobilized membrane.

Claims

[Scope of utility model registration request]

(1) By bringing the immobilized enzyme membrane into contact with a sample solution and electrochemically measuring the increase or decrease in the electrode-detectable substance due to the enzyme reaction, the concentration of the analyte in the sample solution or the involvement of the analyte An immobilized enzyme membrane used in an enzyme electrode for measuring the enzyme activity of an enzyme reaction in which the sample is The side that contacts the solution is made of a porous polymer membrane that excludes polymer substances and allows the substance to be measured to pass therethrough, and a portion that is located between the polymer thin film and the porous polymer membrane and that corresponds to the electrode surface. An immobilized enzyme membrane, characterized in that it has a partial three-layer structure in which an immobilized enzyme is provided only in the periphery of the immobilized enzyme, and the thin polymer membrane and the porous polymer membrane are laminated around the immobilized enzyme.

(2) The immobilized enzyme membrane according to claim 1, wherein the electrode includes a working electrode and a reference electrode, and the immobilized enzyme is provided only in a portion corresponding to the surface of the working electrode.