JPS5942893A - Oxidation decomposition of dimethyl phosphate by microorganism - Google Patents

Abstract

PURPOSE:To carry out the oxidative decomposition of dimethyl phosphate to inorganic phosphoric acid, by using a microbial strain belonging to Pseudomonas genus and capable of oxidatively decomposing dimethyl phosphate. CONSTITUTION:A microbial strain belonging to Pseudomonas genus and capable of oxidatively decomposing dimethyl phosphate, e.g. Pseudomonas AK-2 (FERM- P No.6581) is inoculated in a medium containing dimethyl phosphate, and cultured under aerobic condition at 15-37 deg.C, preferably about 30 deg.C, and 5-8pH, preferably about 6.5pH.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides dimethyl, phosphorus II (hereinafter referred to as DMP) production by microorganisms.
This relates to the oxidative decomposition method of (abbreviated as).

DMP is mainly contained in the wastewater of organic phosphorus pesticide factories, etc., and in the cultivation soil of agricultural crops sprayed with B chemicals, and is a cause of contamination of river basins.

For this reason, a method of avoiding oxidative decomposition of DMP using an activated sludge method is being investigated, but a method of oxidizing and decomposing DMP using pure bacteria has not been attempted.

The present inventors believe that if DMP can be decomposed by pure bacteria, it is not only possible to incorporate it into activated sludge, but also to be able to decompose it using pure bacteria in the future. As a result of searching for such microorganisms, it was discovered that a strain recognized as belonging to the genus Pseudomonas has the ability to oxidize and decompose DMP to inorganic phosphoric acid, and the method of the present invention was implemented.

The microorganisms utilized in the present invention succumb to the genus Pseudomonas;
Microorganisms that have a high oxidative decomposition ability for dimethyl phosphoric acid are used, and a suitable example of such a microorganism is the newly isolated Pseudomonas Δ1<-2.

The mycological properties of Pseudomonas AK-2 are as follows. .

Mycological properties (a) Form 1〉, cell shape and size: Bacillus, 0.8...1. O
Xl, 5-2.5 μmη 2), cell pleomorphism: none 3), motility: right. Right polar flagellum.

/I), sporulation: no 5), Durham staining: negative 6), acid-fastness: no (b) Growth conditions in each of the following locations 1), phosphate buffer (p l-16°8) Broth agar plate containing:
I'm an expert on growing up. Round. The center is slightly raised. Pink. Only the center part is red.

Opacity.

2), Methanol agar deck J8 ground: Beef γ1114 pieces. Round. Gold rim. flat. Pink. Opacity.

3) Contains phosphate buffer (pH 6,8>) Nikun 1 Kanjinslope J8 ground: growth control. Pink color. Opaque.

4) Meat 1 containing phosphate buffer (, pl ~ +6.8)
Agar liquid medium: Normal growth. The liquid becomes cloudy. .

5) Meat juice gelatin puncture culture did not become two liquids.

6), January trout milk: unchanged 1° (C) Physiological properties 1), nitrate reduction: positive 2), UtJ nitrogen reaction: negative 3), to MR death 1: negative 4), Vl [Death 1~: Negative 5), Production of indole: Negative 6), Production of aqueous sulfide: Negative 7), Hydrolysis of starch: Negative 8), Utilization of citric acid: Cory's medium and Christensen's medium Izu (shi Cb use °ru.

9) Inorganic nitrogen source; used for nitrates and salts.

10), Pigment: water-soluble color, water is produced 1° 11), Urease: positive 12), Oxider U: positive 13), Catarage: positive 11 14), Growth range: pl-15~8° temperature 15-37
℃.

15), Attitude towards oxygen: aerobic 16), 0-F eggplant 1-: has weak sugar decomposition ability, and assimilates J and I~, so pf-l rises rapidly and cannot be determined.

17) Production of acid and gas from sugar fu, 'r1: No gas is produced. Acid is produced from D-fructose, D-xylose, and glycerin. L-arabinose, D-glu':]-su, D-nnnnose, D-galactinose,
Acid is not produced from maltose, sucrose, lactose, trehalose, D-sorbitol, D-mannitol, and Y-1-1fjH5).

18), Auxotrophy: None 19), G and C content in DNA: 69.6% 20), Main fatty acids in bacterial cells: 1 unsaturated bond, 46 strands, 18
However, the classification V1 has not been established for the indomitable methanol-assimilating bacteria, and the closest pseudomonies Rosea dust, assimilation smell (°phase) hill. In other words, ``Chickode Eggplant 1'' Pia does not use bu1] pionic acid, citric acid, or alanine at all, but in its home country it uses these to a large extent and shows good growth. -2 (Microbacterium 6th bacterium)
No. 581) and has been deposited with the Institute of Microbiology and Technology, Agency of Industrial Science and Technology.

Japan is characterized by using DMI' as its sole energy source and carbon source, and decomposing it into inorganic phosphoric acid.

Other nutritional sources for wood fungi include the commonly used phosphoric acid-canasim, nitrium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, l! i! An inorganic medium containing ferric acid, etc. is provided. In my home country, the temperature is 15-3
It grows at 7°C, but it is more desirable to culture it at around 30°C.

Also, [)11-) is 5 to F3 during life, but J:
preferably 6.5 (it is better to culture near J, but J8
During nutrition, DMP is decomposed and inorganic phosphoric acid is produced, p
H decreases in C1 hydroxide, helium, ammonia,
It is necessary to adjust and cover the nutrient solution by adding an alkaline agent such as sodium carbonate to lithium as appropriate, but it is decomposed by phosphorus and 21, so its utility value is It's large.

Hereinafter, the present invention will be specifically explained with reference to Examples.

Example

Claims (1)

[Claims] A method for oxidative decomposition of dimethyl phosphate using a microorganism, which is characterized by culturing a microorganism belonging to the genus Pseudomonas and having a high oxidative decomposition ability for dimethyl phosphate in a dimethyl phosphate-containing medium.