JPS5939129B2 - blood collection tube - Google Patents

Description

Detailed Description of the Invention (1) Background of the Invention (Technical Field) The present invention relates to a blood collection tube capable of coagulating collected blood.

When centrifuging serum from collected whole blood, if blood coagulation is insufficient, a barrier with intermediate specific gravity will not be formed after centrifugation, and blood cells may leak into the serum layer when the blood collection tube is placed on its side. , problems such as increased generation of fibrin and limitations on the amount of serum collection may occur.

Furthermore, if a gel barrier is not placed in the blood collection tube in advance, if coagulation is insufficient, fibrin will form in the collected serum after centrifugation, causing it to solidify and become cloudy, making testing almost impossible.

For this reason, conventionally, after blood collection, it takes 45 minutes to 1.5 hours to sufficiently coagulate the blood before centrifugation, but due to individual differences, sufficient coagulation may not be achieved and fibrin may precipitate.

(Prior Art) Such a long coagulation time is unfavorable in terms of serum separation operation and blood test efficiency, and therefore various measures have been proposed to promote blood coagulation.

For example, a method is known in which the inner wall of a blood collection tube is coated with glass powder using a water-soluble polymer, or a method in which a container in which glass powder is dispensed is placed inside the blood collection tube.

(Problems with the Prior Art) However, the former method requires complicated and troublesome steps such as coating and drying, and also uses an organic solvent, which is not favorable in terms of the working environment.

In addition, the latter method requires a container to contain the glass powder, which increases the cost, and also requires dispensing an appropriate amount of glass powder to prevent hemolysis, which causes management difficulties. There's a problem.

Furthermore, since all of the above methods use fine particles such as glass powder, if blood backflow occurs during blood collection,
It cannot be denied that this substance may enter the body through the camera.

■ Purpose of the Invention The present invention was developed in view of the above circumstances.The manufacturing process is simple, product management is easy, and even if there is backflow of blood during blood collection, there is no backflow of foreign substances. There is no fear,
An object of the present invention is to provide a blood collection tube that can easily promote blood coagulation.

That is, the present invention comprises a blood collection tube main body and a hydrophilic continuous porous blood coagulation promoting member with a specific gravity of 1.09 or more, which is anchored to an intermediate portion within the blood collection tube main body, and, if necessary, The present invention provides a blood collection tube characterized in that a barrier for serum separation is housed in the tube in advance.

Furthermore, the present invention provides the above blood collection tube in which the blood coagulation promoting member is sheet-shaped, ring-shaped, or block-shaped, and has a surface area of 0.
．． The present invention provides a blood collection tube having a diameter of 5 to 3.5 d and a size that allows it to be embedded in a blood cell layer after centrifugation.

Furthermore, the present invention provides blood coagulation in the blood collection tube.

The present invention provides a blood collection tube characterized in that the solidification promoting member is coated with a hydrophilic polymer.

Furthermore, the present invention provides the above-mentioned blood collection tube, wherein a serum separation barrier is housed in advance within the blood collection tube body.

The present invention provides a blood collection tube characterized in that:

■Detailed Description of the Invention In addition to the blood coagulation effect, the blood coagulation promoting member used in the present invention must substantially satisfy the following conditions.

In other words, it has good wettability with blood, does not generate eluates that would affect blood tests, has no hemolytic toxicity, and has a specific gravity higher than blood cells (approximately 1.09 or higher).
It must not generate volatile substances that may cause blood clots to adhere to rubber blood collection tubes, etc., it must not cause physical, chemical, or hematological changes due to sterilization using γ-rays, etc., and it must have appropriate flexibility. It also has continuous pores and is relatively smooth (to prevent hemolysis).
In addition, the external shape is such that it can be buried in the blood cell layer after centrifugation, and there is no risk of it flowing back into the body along with the blood through the camera, and the contact area with blood is preferably 0.5 to 0.5. It is desired that the material satisfies conditions such as being 3.5c111 and having a thickness that does not absorb a large amount of blood.

A specific example of a material that satisfies these conditions is a cellulose acetate filter (for example, T manufactured by Toyo Oki Paper Co., Ltd.).
M2R, TM4R), nitrocellululose filters (for example, TM2 and TM4 manufactured by Toyo P Paper Co., Ltd.), polyvinyl formal open-pore materials with a degree of formalization of 60 to 70 (for example, Kanebo Bell manufactured by Kanebo Synthetic Chemical Co., Ltd.) Hydrophilic to nonwoven fabrics such as Eta W3600゜W3610) cut or punched into appropriate sizes, polyester nonwoven fabrics (e.g. 20603FLO manufactured by Unitika), or regenerated cellulose/polyester blended nonwoven fabrics (e.g. 800 manufactured by Fuji Chemicross). A material coated with a sexpolymer may be used.

Examples of hydrophilic polymers include polyvinylpyrrolidone, hydroxypropylmethylcellulose, hydroxyethyl methacrylate, and the like.

The present invention will be described below with reference to illustrated embodiments.

In the figure, 1 is a vacuum blood collection tube, the open end of which is fitted with a rubber stopper 2, and the bottom of which is filled with a thixotropic gel-like substance (for example, a composition mainly composed of silicone and hydrophobic silica, n-α - A barrier 3 made of a composition mainly composed of an olefin/dimethyl maleate copolymer and bentonite (for example, Benton 38 manufactured by NL Industries) is accommodated.

A blood coagulation promoting member 4 is held between the tube walls in the middle portion of the blood collection tube 1 by utilizing its flexibility.

The blood coagulation promoting member 4 is, for example, a cellulose acetate filter with a thickness of about 0.2 mm punched out into a substantially circular shape with a diameter slightly larger than the inner diameter of the blood collection tube 1. As shown in FIG. It is held in the middle part of 1.

When blood is collected using this blood collection tube, the blood is filled into the blood collection tube 1 while coming into contact with or in contact with the blood coagulation promoting member 4, and as a result, blood coagulation is promoted and the blood can be well-prepared within 15 minutes, for example. Blood clot formation can be obtained.

After blood coagulation in this way, as before, 70
By performing centrifugation for about a minute with a centrifugal force of 0 to 100 OG, the barrier 3 can be stably interposed between serum and blood clots, and serum without fibrin precipitation can be obtained.

Fig. 2 and Fig. 3 show examples of shape variations of the blood coagulation promoting member, in which Fig. 2 shows a blood coagulation promoting member 4a formed into a cylindrical shape, and Fig. 3 shows a blood coagulation promoting member formed into a net shape. 4b are shown respectively.

The diameter or longitudinal size of the blood coagulation promoting member 4 is approximately 0.5 mm from the tube inner diameter (for example, in a 10CC blood collection tube, it is usually 141 m±0.5 mm) It is desirable to make it larger.

Note that in the present invention, the blood coagulation promoting member is used by being accommodated in a blood collection tube that does not contain a barrier in advance.

The barrier may be placed within the blood collection tube after blood coagulation.

■Specific effects of the invention As detailed above, this invention does not use fine powder such as glass powder for promoting blood coagulation as in the past, but simply uses a hydrophilic continuous porous material to promote blood coagulation. Therefore, the manufacturing process is simple, quality control is easy, and problems such as foreign matter entering the body due to blood reflux during blood collection can be avoided.

In addition, by making the outer diameter or longitudinal dimension of the blood coagulation promoting member larger than the inner diameter of the blood collection tube, it can be held at an intermediate position within the blood collection tube, and the oil component of the serum separation barrier stored in advance in the blood collection tube can be held at an intermediate position within the blood collection tube. There is no drawback that the absorbing portion loses its hydrophilicity due to being sucked up by the promoting member by capillary action, and such blood collection tubes have excellent practical effects.

[Brief explanation of drawings]

FIG. 1 is a sectional view showing an embodiment of a blood collection tube according to the present invention, and FIGS. 2 and 3 are perspective views showing modified examples of the blood coagulation promoting member used in the present invention. In the figure, 1... blood collection tube, 2... plug, 3
...Barrier, 4, 4a, 4b...
...Blood coagulation promoting member.

Claims (1)

[Claims] 1. A blood collection tube body, and a hydrophilic continuous porous blood coagulation promoting member with a specific gravity of 1.09 or more, which is anchored to an intermediate portion of the blood collection tube body. blood collection tube. 2. The blood collection tube according to claim 1, wherein the blood coagulation promoting member is in the form of a sheet. 3. The blood collection tube according to claim 1, wherein the blood coagulation promoting member is annular. 4. The blood collection tube according to claim 1, wherein the blood coagulation promoting member is in the form of a block. 5. The blood collection tube according to claim 2, 3 or 4, wherein the blood coagulation promoting member has a surface area of 0.5 to 3.5 CIIIL. 6. The blood collection tube according to claim 1, wherein the blood coagulation promoting member is selected from polyvinyl formal continuous pores having a degree of formalization of 60 to 70, cellulose acetate filters, and nitrocellulose filters. 7. The blood collection tube according to claim 1, wherein the blood coagulation promoting member is coated with a hydrophilic polymer. 8. The blood collection tube according to any one of claims 1 to 7, wherein the blood coagulation promoting member has a size that allows it to be embedded in a blood clot layer after centrifugation. 9. The blood collection tube according to any one of claims 1 to 8, wherein the blood collection tube body contains a serum separation barrier in advance.