JPH0128346B2 - - Google Patents
Info
- Publication number
- JPH0128346B2 JPH0128346B2 JP55035215A JP3521580A JPH0128346B2 JP H0128346 B2 JPH0128346 B2 JP H0128346B2 JP 55035215 A JP55035215 A JP 55035215A JP 3521580 A JP3521580 A JP 3521580A JP H0128346 B2 JPH0128346 B2 JP H0128346B2
- Authority
- JP
- Japan
- Prior art keywords
- barrier
- blood
- porous body
- serum
- collection tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000004888 barrier function Effects 0.000 claims description 71
- 210000004369 blood Anatomy 0.000 claims description 57
- 239000008280 blood Substances 0.000 claims description 57
- 230000005484 gravity Effects 0.000 claims description 22
- 239000011148 porous material Substances 0.000 claims description 18
- 238000005119 centrifugation Methods 0.000 claims description 13
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 description 36
- 210000000601 blood cell Anatomy 0.000 description 15
- 239000010410 layer Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 14
- 102000009123 Fibrin Human genes 0.000 description 10
- 108010073385 Fibrin Proteins 0.000 description 10
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 10
- 229950003499 fibrin Drugs 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 238000000926 separation method Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 6
- 229920001971 elastomer Polymers 0.000 description 5
- 229920002635 polyurethane Polymers 0.000 description 5
- 239000004814 polyurethane Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000004800 polyvinyl chloride Substances 0.000 description 4
- 229920000915 polyvinyl chloride Polymers 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000007536 Thrombosis Diseases 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 238000010908 decantation Methods 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 229920005830 Polyurethane Foam Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 229920005672 polyolefin resin Polymers 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 239000011496 polyurethane foam Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- IDCBOTIENDVCBQ-UHFFFAOYSA-N TEPP Chemical compound CCOP(=O)(OCC)OP(=O)(OCC)OCC IDCBOTIENDVCBQ-UHFFFAOYSA-N 0.000 description 1
- 210000002457 barrier cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920005749 polyurethane resin Polymers 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
発明の背景
技術分野
この発明は血液を遠心分離によつて、血液細胞
等の固形成分と血清等の液体成分とに分離するた
めに用いられるバリヤーに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention This invention relates to a barrier used to separate blood into solid components such as blood cells and liquid components such as serum by centrifugation.
血液検査を目的として血液を遠心分離により血
清と血球等の細胞質固形成分とに分離し、血清の
みを採取し、これを分析、検査することがおこな
われている。 BACKGROUND ART For the purpose of blood testing, blood is separated into serum and cytoplasmic solid components such as blood cells by centrifugation, and only the serum is collected for analysis and testing.
先行技術
このように血清を分取する方法として、試験管
に血液を採取したのち、遠心分離し、分離された
血清をピペツトで吸う方法が一般に知られてい
る。しかし、この方法はフイブリンおよび血球を
同時に吸うおそれがある。また、他の方法とし
て、血清と細胞質成分との中間比重を有する物質
たとえばシリコーン／シリカからなるゲル状物質
を試験管内に収容し、遠心分離によつてこのゲル
状物質を血清と細胞質成分との中間に介在させ、
血清成分をデカンテーシヨンによつて分取する方
法も知られている。しかし、この場合もフイブリ
ン等の混入を完全に防止することは困難である。Prior Art As a method for separating serum in this way, a generally known method is to collect blood in a test tube, centrifuge it, and suck up the separated serum with a pipette. However, this method may suck up fibrin and blood cells at the same time. As another method, a gel-like substance made of a substance having an intermediate specific gravity between serum and cytoplasmic components, such as silicone/silica, is placed in a test tube, and this gel-like substance is separated between serum and cytoplasmic components by centrifugation. intervene in the middle,
A method of separating serum components by decantation is also known. However, in this case as well, it is difficult to completely prevent contamination with fibrin and the like.
このような血球成分、フイブリン等の血清等へ
の混入は検査の測定誤差を生じさせるほか器具の
ノズルをつまらせるおそれがあり、好ましくな
い。 Such contamination of blood cell components, fibrin, etc. into serum, etc. is not preferable because it may cause measurement errors in the test and may clog the nozzle of the instrument.
そのため、このような血清への混入を防止し得
る血液分離装置として採血管の内側管壁に摺接す
る大きさの可撓性フイルターに比重調整のための
固形状垂りを結合させ、全体として、比重が1.03
ないし1.09のピストン部材を採血管内に収容する
装置も提案されている（特開昭51−105890）。し
かし、このピストン部材は比重の異なる２種類の
部材、すなわち、通気性部材と固形部材を接着さ
せて用いるものであり、製造上多くの手間を要す
るなど必ずしも満足なものとは云えない。 Therefore, as a blood separation device that can prevent such contamination with serum, a flexible filter sized to slide on the inner tube wall of a blood collection tube is combined with a solid drip for specific gravity adjustment, and as a whole, Specific gravity is 1.03
A device in which a piston member of 1.0 to 1.0 mm is housed in a blood collection tube has also been proposed (Japanese Patent Laid-Open No. 51-105890). However, this piston member uses two types of members with different specific gravities, that is, an air permeable member and a solid member, which are bonded together, and is not necessarily satisfactory, as it requires a lot of time and effort to manufacture.
発明の目的
この発明は上記事情に鑑みてなされたものであ
つて、血清を血球等固形成分から極めて簡単に遠
心分離することができ、しかも分離された血清中
への血球あるいはフイブリン等の混入のおそれが
なく、さらに製造が簡単でコストの軽減を図るこ
とができる血液分離用バリヤーを提供することを
目的とする。 Purpose of the Invention The present invention has been made in view of the above circumstances, and is capable of centrifuging serum extremely easily from solid components such as blood cells, and also prevents contamination of blood cells, fibrin, etc. into the separated serum. It is an object of the present invention to provide a blood separation barrier that is free from danger, is easy to manufacture, and can reduce costs.
すなわち、この発明は気孔率40％以上、直径50
〜400μの連続気孔を有し、使用される採血管内
腔の横断面に対し、上部が実質的に大きい横断面
を有し、下部は実質的に小さい横断面を有する柱
状弾性多孔質体と、該多孔質体の底面に形成され
た硬質層とからなり、全体の比重が1.2ないし1.4
であることを特徴とする血液遠心分離用バリヤー
を提供するものである。 That is, this invention has a porosity of 40% or more and a diameter of 50%.
a columnar elastic porous body having continuous pores of ~400 μ, an upper part having a substantially larger cross-section and a lower part having a substantially smaller cross-section with respect to the cross-section of the lumen of the blood collection tube to be used; and a hard layer formed on the bottom surface of the porous body, and the total specific gravity is 1.2 to 1.4.
The present invention provides a blood centrifugation barrier characterized by:
さらに、この発明は上記採血管内腔の横断面よ
りも大きい横断面を有する該多孔質体上部が血液
遠心分離時に採血管内壁と摺接状態を保持し、そ
の厚みが３mmないし５mmであることを特徴とする
上記バリヤーを提供するものである。 Furthermore, the present invention provides that the upper part of the porous body, which has a cross section larger than the cross section of the inner lumen of the blood collection tube, maintains sliding contact with the inner wall of the blood collection tube during blood centrifugation, and has a thickness of 3 mm to 5 mm. The present invention provides the above-mentioned barrier.
さらに、この発明は硬質層の厚みがそれより上
部の多孔質体の厚みより実質的に小さいことを特
徴とする上記バリヤーを提供するものである。 Furthermore, the present invention provides the above-mentioned barrier, characterized in that the thickness of the hard layer is substantially smaller than the thickness of the porous body above it.
さらに、この発明は外形が截頭円錐状をなして
いることを特徴とする上記バリヤーを提供するも
のである。 Furthermore, the present invention provides the barrier described above, characterized in that the outer shape is a truncated cone.
さらに、この発明は底面の硬質層が硬質の無孔
又は有孔のプラスチツク板または無機質板を上記
弾性多孔質柱状体底面に被着させたものからなる
ことを特徴とする上記バリヤーを提供するもので
ある。 Furthermore, the present invention provides the above-mentioned barrier, wherein the hard layer on the bottom surface is made of a hard non-porous or perforated plastic plate or an inorganic plate adhered to the bottom surface of the elastic porous columnar body. It is.
さらに、この発明は底面の硬質層が硬質のプラ
スチツクを上記弾性多孔質柱状体底部に含浸固化
させたものからなることを特徴とする上記バリヤ
ーを提供するものである。 Furthermore, the present invention provides the above-mentioned barrier, wherein the hard layer on the bottom surface is made of hard plastic impregnated and solidified at the bottom of the elastic porous columnar body.
発明の具体的説明
以下、この発明を図示の実施例を参照して説明
する。 DETAILED DESCRIPTION OF THE INVENTION The present invention will be described below with reference to illustrated embodiments.
第１図および第２図に示すように、本発明に係
わるバリヤー１は連続気孔を有し真比重が血清成
分より大きい截頭円錐状の弾性多孔質柱状体１ａ
と、その小径部分をなす底面に形成された硬質層
１ｂとからなつている。このバリヤー１は第３図
Ａに示すように、その上部が使用される採血管３
内腔の横断面よりも大径につくられ、下部は採血
管３内腔の横断面よりも実質的に小径となつてい
る。 As shown in FIGS. 1 and 2, the barrier 1 according to the present invention is a truncated cone-shaped elastic porous columnar body 1a having continuous pores and having a true specific gravity larger than that of serum components.
and a hard layer 1b formed on the bottom surface of the small diameter portion. As shown in FIG. 3A, this barrier 1 has an upper portion that is connected to a blood collection tube 3 to be used.
It is made to have a larger diameter than the cross section of the lumen, and the lower part has a substantially smaller diameter than the cross section of the lumen of the blood collection tube 3.
従来の血液分離方法あるいは装置と比較して、
この発明のバリヤーの特異な点は特定の連続気孔
を有する弾性体をそのまま相分離材（あるいはバ
リヤー）として使用し得ることである。さらにこ
の発明で従来の技術思想と著るしく異なる点はこ
の弾性体からなるバリヤーの真比重は血清より重
いことを要するが、溶血を生じさせるおそれがな
い範囲内であれば、必ずしも血液中の固形成分相
より軽いことを要しないことである。これは本発
明に係わるバリヤーの全体あるいは主要部が多孔
質体で質量が著るしく小さい（たとえば100〜300
mgの範囲）ことによるものと思われる。従来、こ
の種バリヤーはすべて分離されるべき２相の中間
比重を有するように工夫されていたことからみ
て、このような本発明の技術思想は全く新規な着
想であり、使用し得る材質の範囲を従来と比較し
て著るしく拡大し得ること、バリヤーの比重が従
来のバリヤーと比較して大きいから、小さい遠心
力でも迅速に血清分離をおこなうことが可能であ
ること、などの利点を有する。 Compared to traditional blood separation methods or devices,
A unique feature of the barrier of the present invention is that the elastic body having specific open pores can be used as it is as a phase separation material (or barrier). Furthermore, the present invention is significantly different from the conventional technical concept in that the true specific gravity of the barrier made of an elastic body is required to be heavier than serum, but this does not necessarily mean that the true specific gravity of the barrier is heavier than blood serum as long as it does not cause hemolysis. It does not need to be lighter than the solid component phase. This is because the entire or main part of the barrier according to the present invention is porous and has a significantly small mass (for example, 100 to 300
mg range). In view of the fact that all conventional barriers of this type have been devised to have a specific gravity intermediate between the two phases to be separated, the technical concept of the present invention is a completely new idea, and the range of materials that can be used is It has advantages such as being able to significantly expand the amount of water compared to conventional barriers, and because the specific gravity of the barrier is greater than that of conventional barriers, it is possible to perform serum separation quickly even with a small centrifugal force. .
さらにフイルター効果を利用する従来のバリヤ
ーと比較した場合、構造上、量産性に適している
点で極めて有利である。さらに採血管内壁との接
触面積を大きくすることができるので該内壁に付
着する血球、フイブリン等の除去効果が優れてい
るなどの利点も有する。 Furthermore, when compared with conventional barriers that utilize filter effects, this structure is extremely advantageous in that it is suitable for mass production. Furthermore, since the contact area with the inner wall of the blood collection tube can be increased, it has the advantage of being excellent in removing blood cells, fibrin, etc. adhering to the inner wall.
この発明においてバリヤーの主体をなす弾性多
孔質柱状体としては比重が血清成分より軽いも
の、血球成分より重いものなど広範囲のものを使
用し得る。前者の軽い比重のものを使用した場合
は、バリヤー全体の比重を考慮して硬質層として
適当に重いものを選択すればよい。バリヤー全体
の比重は1.2以上が望ましく、最適な範囲は1.2〜
1.4である。この弾性多孔質柱状体の具体例とし
ては弾性発泡プラスチツク、たとえば発泡ポリウ
レタン、発泡ポリオレフイン、ラバーフオーム
（たとえばシリコーンゴムラテツクス）、ポリ塩化
ビニルフオーム、ポリホルマール樹脂等の弾性多
孔質体からなり、気孔率40％以上、好ましくは97
％〜98％、気孔の大きさ50〜400μ、好ましくは
250〜400μの連続気孔を有するものである。なお
弾性多孔質体は、上記の気孔率と気孔の大きさと
実質的に同等であれば、弾性を有する不織布でも
よい。気孔率および気孔の大きさが小さ過ぎると
血清通過時の抵抗が強くなり、通常の遠心操作
（1000〜1200G、10分間）では十分な血清分離が
得られず、そのため遠心力を上記以上に大きくす
ると溶血が起きることから好ましくない。また、
気孔の大きさが400μ以上であると血清中に血球
等の混入のおそれがあるので好ましくない。ま
た、この場合のバリヤーの25％圧縮硬度
（JISK6401試験方法）が５〜150Kg／cm2のものが
よい。さらに、この発明において用いられるバリ
ヤーは材質的に親水性を有するもの、あるいは親
水化処理によつて親水性を付与されたものが特に
好ましい。これはバリヤーが血液と接触した際
に、その気孔中に血液を速かに浸透させ、バリヤ
ーの移動を容易にするからである。 In the present invention, a wide range of elastic porous columnar bodies can be used as the main body of the barrier, including those whose specific gravity is lighter than serum components and those whose specific gravity is heavier than blood cell components. If the former type of light specific gravity is used, an appropriately heavy hard layer may be selected in consideration of the specific gravity of the entire barrier. The specific gravity of the entire barrier is preferably 1.2 or more, and the optimal range is 1.2~
It is 1.4. Specific examples of this elastic porous columnar body include elastic porous bodies such as elastic foam plastics, foamed polyurethane, foamed polyolefin, rubber foam (such as silicone rubber latex), polyvinyl chloride foam, and polyformal resin. rate of 40% or more, preferably 97
%~98%, pore size 50~400μ, preferably
It has continuous pores of 250 to 400μ. Note that the elastic porous body may be a nonwoven fabric having elasticity as long as the porosity and pore size are substantially the same as described above. If the porosity and pore size are too small, the resistance during serum passage will be strong, and normal centrifugation (1000-1200G, 10 minutes) will not achieve sufficient serum separation. This is not desirable because hemolysis occurs. Also,
If the pore size is 400μ or more, it is not preferable because there is a risk that blood cells etc. may be mixed into the serum. In this case, the barrier preferably has a 25% compression hardness (JISK6401 test method) of 5 to 150 kg/cm 2 . Furthermore, it is particularly preferable that the barrier used in the present invention has a hydrophilic material, or one that has been rendered hydrophilic by a hydrophilic treatment. This is because when the barrier comes into contact with blood, the blood quickly penetrates into its pores, making it easier to move the barrier.
バリヤーの形状としてはバリヤーとともに使用
される遠心分離用採血管の横断面の形状よりも若
干大きい横断面を少なくともその上部に有し、遠
心分離操作時にその大径部の外周縁によつて採血
管の内壁をこするようにして摺動し得るものであ
れば如何なる形状のものであつてもよい。なお、
より好ましくは採血管の内壁との接触部の厚みが
３mmないし５mmとなるようにする。 The shape of the barrier is such that at least its upper portion has a cross section that is slightly larger than the cross section of the centrifugation blood collection tube used with the barrier, and the outer periphery of the large diameter portion of the barrier allows the blood collection tube to be It may be of any shape as long as it can slide against the inner wall of the container. In addition,
More preferably, the thickness of the contact portion with the inner wall of the blood collection tube is 3 mm to 5 mm.
この発明に係わるバリヤーは上述の如く単一の
弾性多孔質体のものをそのまま使用し、その底面
を単に硬質に、するものであるが、さらに外周部
をシリコーンコートしてもよい。 The barrier according to the present invention uses a single elastic porous body as described above, and its bottom surface is simply made hard, but the outer periphery may be further coated with silicone.
なお、バリヤーの主体をなす弾性多孔質体の底
面を硬質にする手段としては該底面に硬質板を貼
着する方法と、該底面部に硬質の材料を含浸させ
固化させる手段とが考えられる。前者の方法とし
ては硬質板の材料としてポリオレフイン系樹脂、
PVC、ナイロン、ポリエステル、ポリカーボネ
ート、ポリウレタン、フツ素樹脂等のプラスチツ
ク板又は無機質板を接着剤、溶着等により接合さ
せる。なお、この硬質板は多孔質であつてもよ
い。たとえばメツシユ状の硬質板を使用すること
は血清収量の点で有利である。 Note that methods for hardening the bottom surface of the elastic porous body that forms the main body of the barrier include a method of attaching a hard plate to the bottom surface, and a method of impregnating and solidifying the bottom surface with a hard material. In the former method, polyolefin resin,
Plastic plates or inorganic plates made of PVC, nylon, polyester, polycarbonate, polyurethane, fluororesin, etc. are joined by adhesive, welding, etc. Note that this hard plate may be porous. For example, the use of mesh-like rigid plates is advantageous in terms of serum yield.
後者の方法としてはたとえばポリオレフイン系
樹脂、PVC、ナイロン、ポリエステル、ポリカ
ーボネート、ポリウレタン等を上記弾性多孔質体
の底部にのみ含浸させ、のちに固化するようにし
てもよい。 As the latter method, for example, polyolefin resin, PVC, nylon, polyester, polycarbonate, polyurethane, etc. may be impregnated only into the bottom of the elastic porous body, and then solidified.
なお、この硬質層の厚みについては特に制限は
ないが、通常0.1mmないし5.0mmの範囲のものが好
ましい。より好ましくは0.1mmないし1.0mmであ
る。 The thickness of this hard layer is not particularly limited, but it is usually preferably in the range of 0.1 mm to 5.0 mm. More preferably, it is 0.1 mm to 1.0 mm.
このようなバリヤーを用いて、血液を遠心分離
する場合の操作としては従来の場合と特に異なる
ところはなく、採血後にバリヤーを採血管内に導
入して遠心分離したのち、血清成分をデカンテー
シヨンによつて容易に採取することができる。 There is no particular difference in the operation when centrifuging blood using such a barrier from conventional methods. After blood collection, the barrier is introduced into the blood collection tube and centrifuged, and then the serum components are decanted. Therefore, it can be easily collected.
第３図Ａ〜Ｃはこの発明に係わる血液分離装置
を用いて、血清を遠心分離する場合の過程を示し
ている。すなわち、第３図Ａに示す如く、採血管
３内に全血２を採取したのち、弾性多孔質材から
なるバリヤー１を管開口部に嵌入し、ついでこれ
らを遠心分離機にセツトして遠心分離を始める
と、バリヤー１が遠心力によつて第３図Ｂに示す
如く、その上部を採血管３の内壁をこするように
して次第に管内部へ降下し、その下端が血液２面
と接触したとき、毛細管現象により血清がバリヤ
ー３の孔内に浸入する。さらに遠心分離をつづけ
ると、さらに徐々に摺動して最終的に第３図Ｃに
示すように血清層４と固形成分層５とのほぼ中間
の位置で保持されるようになる。この場合、血
球、フイブリン等の固形成分はバリヤー１の気孔
中に捕捉され、血清中に混入することはない。こ
れは発泡体よりなるバリヤー１の骨格が複雑な３
次元構造を有するため連続気孔がこれら固形成分
をバリヤー１中に拘束するからである。 FIGS. 3A to 3C show the process of centrifuging serum using the blood separation device according to the present invention. That is, as shown in FIG. 3A, after collecting whole blood 2 into a blood collection tube 3, a barrier 1 made of an elastic porous material is fitted into the tube opening, and then they are set in a centrifuge and centrifuged. When separation begins, the barrier 1 gradually descends into the tube due to centrifugal force, rubbing its upper part against the inner wall of the blood collection tube 3, as shown in FIG. 3B, and its lower end comes into contact with the surface of the blood 2. At this time, serum infiltrates into the pores of the barrier 3 due to capillary action. If the centrifugation is continued further, it will gradually slide further and finally be held at a position approximately midway between the serum layer 4 and the solid component layer 5, as shown in FIG. 3C. In this case, solid components such as blood cells and fibrin are captured in the pores of the barrier 1 and do not mix into the serum. This is because the framework of the barrier 1 made of foam is complex 3
This is because the continuous pores restrict these solid components in the barrier 1 due to the dimensional structure.
このようにしてバリヤー１はその弾性によつて
採血管３の内壁をこするようにして所定位置まで
比較的ゆつくり降下するから、この内壁に付着す
る血球、フイブリン等をほぼ完全にぬぐい取るこ
とができ、これら血球、フイブリン等の固形成分
を含まない血清を得ることができる。この層間位
置に停止したバリヤー１はその弾性によつて採血
管３の内壁に密着し、この内壁を押圧した状態に
保たれているからデカンテーシヨンによつて血清
部分のみを分取することができる。 In this way, the barrier 1 rubs the inner wall of the blood collection tube 3 due to its elasticity and lowers relatively slowly to a predetermined position, thereby almost completely wiping out blood cells, fibrin, etc. adhering to the inner wall. It is possible to obtain serum that does not contain solid components such as blood cells and fibrin. The barrier 1, which is stopped at this interlayer position, adheres tightly to the inner wall of the blood collection tube 3 due to its elasticity and is kept pressed against the inner wall, making it possible to separate only the serum portion by decantation. can.
この発明で用いられるバリヤーは上記例の如く
採血後遠心分離時に採血管内に挿入してもよい
が、そのほか採血管内に予め収容してもよい。第
４図はその一例を示すもので、内部が真空に保た
れた真空採血管１１内に環状硬質層１２を底部に
被着したバリヤー１３がゴム栓１４を介して保持
されている。すなわち、ゴム栓１４は先端に凹部
１５を有し、他方、バリヤー１３にも上面にこの
凹部１５よりやや大きい外径の截頭円錐状突起１
６が形成されていて、その凹部１５内にこの突起
１６が嵌挿、保持されていて、採血時このゴム栓
１４に針を挿通させてもバリヤー１３が離脱され
ないようになつている。 The barrier used in the present invention may be inserted into the blood collection tube during centrifugation after blood collection as in the above example, but it may also be stored in the blood collection tube in advance. FIG. 4 shows an example of this, in which a barrier 13 having an annular hard layer 12 attached to the bottom is held via a rubber stopper 14 in a vacuum blood collection tube 11 whose interior is kept under vacuum. That is, the rubber plug 14 has a recess 15 at its tip, and the barrier 13 also has a truncated conical projection 1 on its upper surface with an outer diameter slightly larger than the recess 15.
6 is formed, and this protrusion 16 is inserted and held in the recessed part 15, so that the barrier 13 will not come off even if a needle is inserted through this rubber stopper 14 during blood collection.
その他、バリヤーを採血管内に予め固定する方
法として採血管あるいはバリヤー自体との形状と
関連させ任意の手段をとることができよう。たと
えば、両端をゴム栓で密封した採血管に対し、血
液導入側と反対側端部に固定するようにしてもよ
い。 In addition, as a method for pre-fixing the barrier within the blood collection tube, any method may be used depending on the shape of the blood collection tube or the barrier itself. For example, it may be fixed to the end opposite to the blood introduction side of a blood collection tube whose both ends are sealed with rubber stoppers.
実施例 １
第１図に示したバリヤー１を用いて血液から血
清を採取する実験を行つた。バリヤー１は弾性多
孔質体１ａと気孔率98％、気孔の大きさ300μ、
比重1.2、JISK−6401（昭和49年制定）の試験方
法による25％圧縮硬度20Kg／cm2、バリヤーのセル
数約75ケ／25mmの発泡ポリウレタンを用いた。こ
の発泡ポリウレタンは、その骨格組織が複雑な３
次元構造を示すような、連続通孔を有し、かつ、
血清の通過抵抗を低くするため、発泡の際に孔の
周囲に形成される膜状物質を熱で融解処理するこ
とにより除いてある。この種の発泡ポリウレタン
はたとえば特公昭41−752で開示されている方法
でつくることができる。Example 1 An experiment was conducted to collect serum from blood using the barrier 1 shown in FIG. Barrier 1 is made of elastic porous body 1a with a porosity of 98% and a pore size of 300μ.
Foamed polyurethane with a specific gravity of 1.2, a 25% compression hardness of 20 kg/cm 2 according to the JISK-6401 (established in 1970) test method, and a barrier cell count of approximately 75 cells/25 mm was used. This polyurethane foam has a complex skeletal structure.
having continuous pores exhibiting a dimensional structure, and
In order to reduce the resistance to passage of serum, the film-like substance formed around the pores during foaming is removed by melting with heat. This type of polyurethane foam can be produced, for example, by the method disclosed in Japanese Patent Publication No. 41-752.
この弾性多孔質体１ａは截頭円錐状をなし、上
部の大径部の直径が15.5mm、下部小径部の直径が
12.8mm、高さが９mmであつた。この小径部の底面
に繊維糸径450μからなる14メツシユのテトロン
メツシユ（比重1.38、例えばNBC工業(株)の商品
名「TB15」）をポリウレタン系接着剤を介して
貼着したサンプルと、厚さ200μの比重1.4の硬質
ポリ塩化ビニルフイルムを、接着剤を介して底面
に貼着したサンプルと、この底面部に２液性のポ
リウレタン樹脂を厚さ約１mm含浸させ、ついで乾
燥硬化させたサンプルとをつくつた。 This elastic porous body 1a has a truncated conical shape, the diameter of the upper large diameter part is 15.5 mm, and the diameter of the lower small diameter part is 15.5 mm.
It was 12.8mm and the height was 9mm. A sample in which 14 meshes of Tetron mesh (specific gravity 1.38, for example, trade name "TB15" manufactured by NBC Kogyo Co., Ltd.) made of fiber yarn diameter 450 μm was attached to the bottom surface of this small diameter part via polyurethane adhesive, and A sample in which a rigid polyvinyl chloride film with a specific gravity of 1.4 and a thickness of 200μ was adhered to the bottom surface with an adhesive, and a sample in which the bottom surface was impregnated with a two-component polyurethane resin to a thickness of approximately 1 mm, which was then dried and cured. I made this.
内径13.6mmの10ml用採血管３に採血したのち、
常温で約60分放置したのち、上記バリヤー１を採
血管３の上部に挿入し、ついで採血管の中央部の
遠心力が約1200G（バリヤーの上面に対しては約
1000G）になるようにして遠心器により遠心を10
分間行つた。 After collecting blood into a 10ml blood collection tube 3 with an inner diameter of 13.6mm,
After leaving it at room temperature for about 60 minutes, insert the barrier 1 into the top of the blood collection tube 3, and then apply a centrifugal force of about 1200G at the center of the blood collection tube (approximately 1200G against the top of the barrier).
Centrifuge in a centrifuge at 1000G) for 10
I went for a minute.
この結果すべてのサンプルのバリヤー１は、血
餅の上端を押さえつけるような形で血餅と血清の
中間位置に位置した。この採血管を肉眼で観察し
たところ、血清中にフイブリンおよび血球が全く
といつてよいほど認められず、デカンテーシヨン
により他の容器に血清を移しかえた後も、同様で
あつた。また、血餅の上層の浮遊血球やフイブリ
ンはバリヤー１の連続通孔内に捕捉されたままに
なつていることが判明した。このようにして採取
された血清の収量はいずれも約4.5mlであり、分
離した血液のほぼ全量を得ることができた。 As a result, the barrier 1 of all samples was located at an intermediate position between the blood clot and the serum so as to press down on the upper end of the blood clot. When this blood collection tube was visually observed, no fibrin or blood cells were found in the serum, and this was the case even after the serum was transferred to another container by decantation. It was also found that floating blood cells and fibrin in the upper layer of the blood clot remained trapped within the continuous holes of the barrier 1. The yield of serum collected in this way was about 4.5 ml in each case, and almost the entire amount of separated blood could be obtained.
発明の具体的作用効果
この発明に係わるバリヤーは上述の如く単に弾
性多孔質体をそのまま使用し小径をなす底面部に
硬質層を設けるだけの簡単な構造のものであるか
ら、たとえば弾性多孔質シートの一方の面に硬質
のプラスチツクシートを貼着するか、あるいは硬
質のプラスチツク溶液を所定の厚みに含浸させた
のち、截頭円錐状に打ち抜くことのできる任意の
打ち抜き機を使用して最終の所望形状に直接成形
することができ、従来のフイルターを使用したバ
リヤーと比較して製造上極めて簡単であり、製造
コストも安価となる。 Specific Effects of the Invention As mentioned above, the barrier according to the present invention has a simple structure in which the elastic porous body is simply used as is and a hard layer is provided on the bottom portion having a small diameter. After pasting a hard plastic sheet on one side of the It can be directly molded into a shape, making it extremely simple and inexpensive to manufacture compared to conventional filter-based barriers.
さらに、この発明に係わるバリヤーは上述の如
く底部を硬質にしたから遠心分離操作時におい
て、バリヤーの降下時のバリヤーの横転、ねじれ
等の変形を防止することができ、安定した状態で
降下する。さらに上述の如く底部の硬質層を弾性
多孔質体の比重より大きな比重を有するもので形
成し、あるいは同比重のものを含浸させて形成し
た場合にはバリヤー全体の重心が著るしく低くな
り、これによつて遠心分離操作時のバリヤーの正
常な向きでの降下動作をさらに確実にしている。 Further, since the barrier according to the present invention has a hard bottom as described above, it is possible to prevent deformation such as overturning or twisting of the barrier when the barrier is lowered during centrifugation operation, and the barrier descends in a stable state. Furthermore, as mentioned above, if the bottom hard layer is formed of a material having a specific gravity greater than that of the elastic porous material, or is formed by impregnating it with a material of the same specific gravity, the center of gravity of the entire barrier becomes significantly lower. This further ensures that the barrier is lowered in the correct orientation during centrifugation.
第１図は本発明の一実施例に係わる血液分離用
バリヤーの斜視図、第２図は第１図の−線に
沿う断面図、第３図Ａ〜Ｃは本発明に係わるバリ
ヤーを使用して血清分離をおこなう操作を工程順
に示す採血管の断面図。第４図は本発明に係わる
バリヤーの使用態様の例を示す断面図である。
図中、１……バリヤー、１ａ……弾性多孔質
体、１ｂ……硬質層、２……血液、３……採血
管、４……血清、５……血球成分、１１……真空
採血管、１２……環状硬質層、１３……バリヤ
ー、１４……ゴム栓、１５……凹部、１６……突
起。
FIG. 1 is a perspective view of a blood separation barrier according to an embodiment of the present invention, FIG. 2 is a sectional view taken along the line - in FIG. 1, and FIGS. FIG. 3 is a cross-sectional view of a blood collection tube showing steps for performing serum separation in the order of steps. FIG. 4 is a sectional view showing an example of how the barrier according to the present invention is used. In the figure, 1... Barrier, 1a... Elastic porous body, 1b... Hard layer, 2... Blood, 3... Blood collection tube, 4... Serum, 5... Blood cell component, 11... Vacuum blood collection tube. , 12... Annular hard layer, 13... Barrier, 14... Rubber stopper, 15... Recess, 16... Protrusion.
Claims (1)
を有し、使用される採血管内腔の横断面に対し、
上部が実質的に大きい横断面を有し、下部は実質
的に小さい横断面を有する柱状弾性多孔質体と、
該多孔質体の底面に形成され、厚みが該多孔質体
の厚みより実質的に小さい硬質層とからなり、全
体の比重が1.2ないし1.4あることを特徴とする血
液遠心分離用バリヤー。 ２ 該多孔質体上部は血液遠心分離時に採血管内
壁と摺接状態を保持し、その厚みが３mmないし５
mmである特許請求の範囲第１項記載のバリヤー。 ３ 全体の外形が截頭円錐状である特許請求の範
囲第１又は２項記載のバリヤー。 ４ 硬質層が硬質プラスチツク板を該多孔質体底
面に被着させたものからなる特許請求の範囲第１
又は２項記載のバリヤー。 ５ 硬質層が硬質プラスチツクを該多孔質体底面
部に含浸固化させたものからなる特許請求の範囲
第１又は２項記載のバリヤー。[Claims] 1. Having a porosity of 40% or more and continuous pores with a diameter of 50 to 400 μ, with respect to the cross section of the lumen of the blood collection tube used,
a columnar elastic porous body having an upper portion having a substantially large cross section and a lower portion having a substantially small cross section;
A barrier for blood centrifugation, comprising a hard layer formed on the bottom surface of the porous body and having a thickness substantially smaller than the thickness of the porous body, and having an overall specific gravity of 1.2 to 1.4. 2 The upper part of the porous body maintains sliding contact with the inner wall of the blood collection tube during blood centrifugation, and has a thickness of 3 mm to 5 mm.
Barrier according to claim 1, which is mm. 3. The barrier according to claim 1 or 2, wherein the entire outer shape is a truncated conical shape. 4 Claim 1 in which the hard layer consists of a hard plastic plate adhered to the bottom surface of the porous body
Or the barrier described in item 2. 5. The barrier according to claim 1 or 2, wherein the hard layer comprises hard plastic impregnated into the bottom surface of the porous body and solidified.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3521580A JPS56130656A (en) | 1980-03-19 | 1980-03-19 | Barrier for blood centrifugation |
| EP19800101547 EP0017127B1 (en) | 1979-03-23 | 1980-03-24 | A method for separating blood and a barrier device therefor |
| AU56786/80A AU542204B2 (en) | 1979-03-23 | 1980-03-24 | Separating blood |
| DE8080101547T DE3069996D1 (en) | 1979-03-23 | 1980-03-24 | A method for separating blood and a barrier device therefor |
| AT80101547T ATE11378T1 (en) | 1979-03-23 | 1980-03-24 | METHOD OF FRACTING BLOOD AND SEPARATION DEVICE THEREFOR. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3521580A JPS56130656A (en) | 1980-03-19 | 1980-03-19 | Barrier for blood centrifugation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56130656A JPS56130656A (en) | 1981-10-13 |
| JPH0128346B2 true JPH0128346B2 (en) | 1989-06-02 |
Family
ID=12435615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3521580A Granted JPS56130656A (en) | 1979-03-23 | 1980-03-19 | Barrier for blood centrifugation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56130656A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4290060A1 (en) | 2022-06-08 | 2023-12-13 | Ktm Ag | A cooling device |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4443345A (en) * | 1982-06-28 | 1984-04-17 | Wells John R | Serum preparator |
| JPS59119263A (en) * | 1982-12-25 | 1984-07-10 | Sonobe Kazunari | Serum separating method and auxiliary means for separating serum |
| WO1996024058A1 (en) * | 1995-01-30 | 1996-08-08 | Niigata Engineering Co., Ltd. | Component separation member and component separator equipped with said member |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51105890A (en) * | 1974-08-09 | 1976-09-20 | Becton Dickinson Co | |
| JPS5344083A (en) * | 1976-09-30 | 1978-04-20 | Heimreid Ken | Facilitating method and apparatus for blood analysis |
| JPS5513657B2 (en) * | 1975-09-25 | 1980-04-10 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5746030Y2 (en) * | 1978-07-11 | 1982-10-09 |
-
1980
- 1980-03-19 JP JP3521580A patent/JPS56130656A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51105890A (en) * | 1974-08-09 | 1976-09-20 | Becton Dickinson Co | |
| JPS5513657B2 (en) * | 1975-09-25 | 1980-04-10 | ||
| JPS5344083A (en) * | 1976-09-30 | 1978-04-20 | Heimreid Ken | Facilitating method and apparatus for blood analysis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4290060A1 (en) | 2022-06-08 | 2023-12-13 | Ktm Ag | A cooling device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56130656A (en) | 1981-10-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4294707A (en) | Method for separating blood and a barrier device therefor | |
| EP0017127B1 (en) | A method for separating blood and a barrier device therefor | |
| US3960727A (en) | Apparatus and method for isolating soluble blood components | |
| US4131549A (en) | Serum separation device | |
| US3814248A (en) | Method and apparatus for fluid collection and/or partitioning | |
| WO2011069145A2 (en) | Blood collection tube with separation barrier | |
| KR100446030B1 (en) | Blood filtration device | |
| EP2264453B1 (en) | Method for filtering blood | |
| US3931018A (en) | Assembly for collection, separation and filtration of blood | |
| JP3890066B2 (en) | Blood separation instrument and blood separation device | |
| JP4885134B2 (en) | Blood separation device and method for separating liquid component fraction from whole blood | |
| EP0040022A2 (en) | Filter element for serum separating apparatus | |
| US3687296A (en) | Fluid separator | |
| EP3219784A1 (en) | Collection of suspended cells using a transferable membrane | |
| JPS63224703A (en) | Membrane separator for component of liquid sample | |
| JP2022164867A (en) | biological fluid separation device | |
| US6220453B1 (en) | Blood filter unit | |
| EP0937981A2 (en) | Method of separating serum from whole blood | |
| JPH0128346B2 (en) | ||
| US6328167B1 (en) | Blood filter cartridge | |
| JPH0533346B2 (en) | ||
| JP2003518248A (en) | Microtiter plate and method of manufacturing the same | |
| JPH0622958U (en) | Blood separation member | |
| JP2000180444A (en) | Hemofilter | |
| JP2000081432A (en) | Haemofiltration unit |