JPS5928495A - Method and apparatus for detecting pollutants - Google Patents
Method and apparatus for detecting pollutantsInfo
- Publication number
- JPS5928495A JPS5928495A JP13679982A JP13679982A JPS5928495A JP S5928495 A JPS5928495 A JP S5928495A JP 13679982 A JP13679982 A JP 13679982A JP 13679982 A JP13679982 A JP 13679982A JP S5928495 A JPS5928495 A JP S5928495A
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- JP
- Japan
- Prior art keywords
- culture
- culture medium
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
不発明による汚染’+th xの検出のための方法及び
≠厘は食料品工業及び類似の工業分野の工場におけるイ
:双生物衛生検査のための、液体からの試l#+探城に
も試料に葛1れている微生物の培養にも役立つ。DETAILED DESCRIPTION OF THE INVENTION A method for the detection of non-inventive contamination '+th #+It is useful for both castle exploration and culturing of microorganisms found in samples.
汚染物質の検出のためには製品の又は器具乃芋装置q1
部分の病原l負値の特性について報告できる≧4さ1な
方法か公知である。しかし原則とし℃検出はいくつかの
作業段階において行なわれる。For detection of contaminants, product or equipment equipment q1
There are well-known methods that can report on the characteristics of negative values for pathogenicity of a part. However, as a rule, °C detection is carried out in several work steps.
実験室における液体又は懸濁孜からの特定の容積の試料
の採取は、試料の対応の区分をもってノー切な固形及び
又は液体培養基土で培養を行ILうため誠凶の器具(た
とえばベット)によって容器から行なわ才りる。この゛
容器はたとえば飲料用の瓶又は輸送用容器とすることか
でき、これに滅菌の条件下において実験室外で試料を満
たす。顕微屓)の像による試料判1′Lピは商度の汚染
の場合のみ0]能である。ハ「扱の°容器の個数及び壌
俟窒気との接触によって二次汚染の危険か存在している
。The collection of a specific volume of a sample from a liquid or suspension in the laboratory is carried out using appropriate equipment (e.g. a bed) in order to culture it in solid and/or liquid culture medium without cutting the corresponding compartments of the sample. Let's do it from now on. The container can be, for example, a beverage bottle or a transport container, and is filled with the sample outside the laboratory under sterile conditions. The sample size (1'L) obtained by microscopic imaging is only 0] in the case of commercial contamination. There is a risk of cross-contamination due to the number of containers handled and contact with nitrogen.
たとえば臥科の保存性検査の保存性検査のためには険め
て僅かな個数においても汚染物質の恢出は樵要である。For example, in order to test the shelf life of bedbugs, it takes a woodcutter to find contaminants even in a very small number.
この目的のためには大容積の試料を膜で戸別してこれを
固形の培養基上にもたら丁。For this purpose, a large volume of sample is separated by membrane and brought onto a solid culture medium.
しかL ’=養法にめっては、弱い病1余肉は固形培養
基上では液状培養基中よりI成長か遅いので満足な結果
は早くとも3日乃全4日間郷化させた后でないと4を認
できない。液状培養基とは公知のコツホの平版培養にも
めてはまる。However, when it comes to the L' = feeding method, weakly diseased meat grows slower on a solid culture medium than in a liquid culture medium, so a satisfactory result can only be obtained after incubation for at least 3 to 4 days. I cannot accept 4. The liquid culture medium also applies to the well-known lithographic culture of Kotsuho.
膜濾過で戸別された微生物の染色及び引続いてのそれら
の噛倣御下でのit敢及び特徴づけはよく装備された作
画J3rでのみ町1ヒであり対応の適格性を必要とする
。そのほか直接n[砿では微生物の生、夕すの判別の0
」能性に限界かめる。The staining of microorganisms separated by membrane filtration and their subsequent incubation and characterization are only possible in a well-equipped J3R and require corresponding qualifications. In addition, direct n [0 for determining microbial life and evening
” Limit your potential.
在米の方法の屯央な欠点はさらに、汚染度のほかにIT
3」時に汚染(人生物の諸性性(ガス発生、醗酵活性、
酸生成、嫌気性情vFFの成長ンも測定1−ることかで
きないことにある。In addition to the degree of contamination, the major drawback of the US method is that
3) Contamination (various properties of living organisms (gas generation, fermentation activity,
Acid production and anaerobic VFF growth are also difficult to measure.
食料品工業及び類似の工業分封において爪上である病原
酌については好気性粂件Fにガス及び酸を生じならびに
ll粗気性条件Fに成長する能力を1Fj1時に立ci
+h−4ることは績にめて爪髪でめる。In the food industry and similar industrial packaging, the ability to produce gases and acids in aerobic conditions and to grow in aerobic conditions is recognized for pathogens that are on the nails.
+h-4.
(lE来の方法姓−1絶ってはガス発生は定性的に検出
いtLイ)か、べいは別f1^1の培曽装置1(たとえ
ば小醗目f狛ンを必−ψlと1−る。在米の方法は実験
室r1イ〔rlに置r;J a 7また器具及び培養基
のイも備を負荷8せる。こ)しによって小暇、イ艶の作
業、方では負召[か大きくなイ〕0
一亡のほか1・Xq−からは採血及び血液試料の隋后処
理のための力l)二及びシステムが公知である。(In the previous method, the gas generation must be detected qualitatively.) Or, it is better to use a separate f1^1 culturing device (for example, it is necessary to set the The method used in the United States is to place equipment and culture media in the laboratory. [Kabigai] 0 In addition to 1.Xq-, a system for blood collection and processing of blood samples is known.
西独公1’i[l ’F)肝出a 第3536061号
及び第2F335101号によると思場の削りIll’
<に仲人された41μ肯システムに試料J!:!搬川′
咎用と1−て僅立つ或いは生化学反応月]乃全用書用容
器とL″′C使用司能の可能に^窒とした小試料管を接
4fするtiJ能性かめる。西独公開性ぎf出願第24
11651号によると採血システム内部に感圧性、ガス
不透過性障!γを通ってのカス発生か示してめり、ガス
分析装隨俵軟の可能性がめる。According to No. 3536061 and No. 2F335101, West German Duke 1'i [l 'F) Ill'
Sample J to the 41μ positive system that was brokered by <! :! Carrier'
For example, if a small sample tube filled with nitrogen is connected to a small sample tube filled with nitrogen, it is possible to use a full document container and a small sample tube filled with nitrogen.West German openness GiF Application No. 24
According to No. 11651, there is a pressure-sensitive, gas-impermeable obstruction inside the blood collection system! It was shown that scum was generated through γ, and the possibility that the gas analyzer was loose was raised.
西独公開時WF出願第3012056号記載の敗血症診
断のための棋合採血培誉システムは液状及び固形培養基
上での同時培介が司Hしであり、血液は採取システムを
経て定線されて血液培養システムの典窒の又はカス充填
の容器内へ吸入されその際にl浅状培養4vcも固形培
養基にも接種さnる。The blood sampling and culturing system for diagnosing sepsis described in West German WF Application No. 3012056 is capable of simultaneous culturing on liquid and solid culture media, and the blood is collected through the sampling system in a fixed line. The shallow culture 4vc is then inoculated into the solid culture medium as well when it is aspirated into the nitrogen-filled or sludge-filled vessel of the culture system.
P)lによって左右される指示桑が成長と結合している
酸化す兄を可視とする。P) Visible is the oxidized brother that indicates mulberry, which is controlled by l, and is combined with growth.
褥化はシステムを斜めに置いて行なわれ、その際採取挿
管は1戚菌のプラスチック栓で閉じたままである。しか
しこのシステムは食料品工業の分野には応用不能である
。Decubation is performed with the system placed obliquely, while the collection cannula remains closed with a 1-family plastic stopper. However, this system is not applicable to the food industry.
不発明の目的は食料品工業及び曲連の分野の製品及び迫
真の微生物的調査のための方法及び装置でめって従来の
p4決法の欠点がなく、これによつ℃′足絹的に汚染度
及び汚染物質の貞要な諸/l’η性の1i3I速ノ↓1
9合の検出が使い棄ての容器とし″′C使用する賜金に
もjjJ能であるものを作り出すことにある。The object of the invention is to develop a method and apparatus for the food industry and food industry field of products and realistic microbiological investigation, which rarely have the drawbacks of the traditional P4 determination method, thereby making it possible to The degree of contamination and the various characteristics of pollutants/l'η characteristics 1i3I speed ↓1
The purpose of the detection of the 9th cup is to create something that can also be used as a disposable container.
本発明には、f取生物学的検査をより確実に摺威し10
」時に作業上の筐た時間的な負担を軽減する方法を商光
りならびに試料採取及び培養の必髪な伎龍丁べてを統合
して対応の液状及び又は同形培養4:を用いる1県に液
体中の汚染物質の乃至公知の方法に従って液体中にi%
?4 ?婦させた微生物の検出が51龍であるようにす
る装置纜をもたらりという峰へか根拠になっている。The present invention has the following features:
``At times, we have developed a method to reduce the time-consuming burden of work by integrating all of the essential aspects of sample collection and culture into one prefecture using liquid and/or isomorphic culture. i% of contaminants in the liquid or in the liquid according to known methods.
? 4? This is the basis for the development of a device that allows the detection of microorganisms caused by female microorganisms to be 51 years old.
本発明による方法はとくに細菌、酵母及び糸状四の検出
VC、;t、Q しており、その場合液状試料は定電し
て減圧の及び又はqq妹なガス充填(たとえばCO,、
Nt)の腹合試料採取、培養容器内へ吸入され、その除
に液状培養基と混合される。The method according to the invention is particularly suitable for the detection of bacteria, yeasts and filamentous substances, in which the liquid sample is electrostatically charged under reduced pressure and/or filled with a small gas (e.g. CO,...
Abdominal sampling of Nt) is aspirated into a culture vessel, whereupon it is mixed with a liquid culture medium.
この装置の別個の区分には一つ又はいくつかの固形培養
基を入れ℃おくことができ、よって泥状培養基と混合さ
れた試料は同時に液状及び固形培養基(1個乃芋数イ[
^1)に接独1−ることができる。Separate compartments of this device can contain one or several solid culture media at °C, so that the sample mixed with the slurry culture medium can be mixed simultaneously with the liquid and solid culture media (1 piece to 100 °C).
^1) can be attached to Germany.
特矩の温良において容器を框直の敷設にして気生物の培
養を行ない、その成長は液状培養基の 濁により乃至固
形培養基上の泉落又田芝状成長によQ′Ot藺できる
汚染微生物の酸生成能力はPHによって左右される(F
i示4シス1ムとくにブロムクレゾール緑(3’、5’
、+1’、5’、テトラブロムmクレゾールスルホンフ
タレインノ又はブロムフェノール〃(テトラブロムフェ
ノールヌルホンフタレイン)の変色により0]祝とされ
る。本発明によりこの方法は餓生l吻のカス生成を、容
器がl放の神官をF向にして適宜な架台に垂直の姿勢に
固定されてカス発生に璃切な容積の培養基微生物懸濁液
が容器から排斥され、これを校正ずみの、1−2しくは
滅菌の、滅菌綿栓で閉じた小管内で側矩するようにして
、検出することを%徴とする。Aerial organisms are cultured in a special rectangular warm environment by placing the container straight on the wall. Acid production ability is influenced by pH (F
I show 4 systems, especially bromcresol green (3', 5'
, +1', 5', due to discoloration of tetrabrom-cresolsulfonphthalein or bromophenol (tetrabromophenol-nurphonphthalein). According to the present invention, this method prevents the production of starving proboscis by fixing the container in a vertical position on a suitable pedestal with the priest facing F, and adding a sufficient volume of culture medium microorganism suspension to prevent the production of scum. The % sign is detected by being expelled from the container and placed in a calibrated, 1-2 or sterile, canalicular tube closed with a sterile cotton stopper.
本発明によりこの方法はそのほか、汚染微生物の諸%性
たとえば病原鋺含南見、カス発生、醗酵活性又は酸生成
が同時に並イーjL″′c求められることをl特徴と−
(−る。よって使用された生産酵母の醗酵活性の試験も
本方法に従って実施できる。In addition, the method according to the invention is characterized in that various properties of contaminating microorganisms, such as pathogenic microorganisms, scum generation, fermentation activity or acid production, can be determined at the same time.
(-) Therefore, testing of the fermentation activity of the production yeast used can also be carried out according to this method.
別個の真青のためには小管状受器からなり培養層にc仮
状及び又は固形の培養基からなり試料を採取−3″る〇
本発明による装置、は試料採取培養装置aの挿管が同時
に訓育すべき液体のための吸入枝管とし′1:またカス
発生の際に排斥寧れる培養基病原菌層i rll Qの
ための〃IL出枝ちとしても形成してあり昭ましくは直
径が311n未満であり、滅菌の栓をMえ℃目盛つき6
111定容器内に突入して保持されることを’II 徴
とする。そのほかこの県政は一つ又はいくつかの固)1
ソ培養基のための容器が液状培鉾基のための容器の外部
に望ましくは区分さ7’した容器として!(突の又はガ
ス充填の室のかたわらに配置道してめ91I!IJ形培
養基用の支持部が設vjて・ろることを特徴とする。For a separate tube-shaped receptacle, the culture layer is made of a temporary and/or solid culture medium, and the sample is taken - 3''. It is also formed as an inlet branch pipe for the liquid to be cultivated, and has a diameter of 311 nm. The sterile stopper is less than 6 degrees Celsius with a scale of M.
111 It is a 'II characteristic that the object is plunged into a fixed container and held there. In addition, this prefectural government has one or several
The container for the liquid culture medium is preferably separated 7' from the outside of the container for the liquid culture medium! (It is characterized by the fact that a support section for the IJ type culture medium is installed next to the bulge or gas-filled chamber.
本発明による方法及び装置の利点は本発明がガス元生性
及び酸生成性微生物を同時に検出することを0]能にす
ることにある。提案されている公知の方法と違って本方
法はそのことによつ″′C醗酵及び飲料工業/jらびに
劇似の工業分野において自利に応用される。An advantage of the method and device according to the invention is that the invention allows simultaneous detection of gas-producing and acid-producing microorganisms. In contrast to the proposed known processes, the process is therefore advantageously applicable in the fermentation and beverage industry and in similar industrial fields.
公知の装置に比べて別個のガス採棺装屓は必襞がない。Compared to known devices, a separate gas sampling casing is not required.
カス発生は培養基が試料採取枝管から逸出することによ
って示されるからである。This is because scum formation is indicated by the escape of the culture medium from the sampling branch.
公知の方法とは違って醗酵活性し母の存在の場合に郷化
中に両層の真青のための試を[の採取ができる。Unlike known methods, when the fermentation is active and the mother is present, it is possible to sample both layers for true blue color during fermentation.
このシステムは汚染微生物の検出のみでなく生産酵母の
醗酵活性の確認のためにも適している。This system is suitable not only for detecting contaminating microorganisms but also for confirming the fermentation activity of production yeast.
16J形培養基のための体は装置の外部Vこ配直しであ
る。固形培養基の対応の接槙の后VCは培養基を立てて
郷化する。従って染洛生戚はただちに祝紹できる。この
装置の別のオロ点は在来の方法に比べて、請査丁べき培
養基を直接採取によりたとえば試料採取の目的で膜を穿
刺して採取−g−るので、試料の汚染はほとんど起り舟
ないことにある。The body for the 16J type culture medium is a rewiring to the outside of the device. After attaching the solid culture medium, the VC is grown into a culture medium. Therefore, some Rakusei relatives can be congratulated immediately. Another advantage of this device is that compared to conventional methods, the culture medium to be tested is collected directly, for example by puncturing the membrane for sample collection purposes, so contamination of the sample rarely occurs. It's in the absence of it.
本発明による方法及び装置を以下実測tすについて示し
i兄明する。The method and apparatus according to the present invention will be explained below with reference to actual measurements.
(清涼飲料中の11ζl酵性及び酸生成性有磯体の同時
検出の場合には汚染物質の調査に先立って一般に威生物
学的訓食の除に龍常行なわれるとおり飲料の脱炭酸を行
なわなくてはなら7.Cい。(In the case of simultaneous detection of 11ζl fermentable and acid-forming minerals in a soft drink, the beverage should be decarboxylated prior to the contaminant investigation, as is generally done in biochemical diets. If I don't have it, it's 7.C.
調査区マ・+に1ハシ、餡の指示楽溶峨を加え、その際
には採取培養システムの培・M基中に用いである指示薬
が用いられる。試料には成田の水酸化ナトリウム規定溶
成を加えて、付イ、Eしてめる酸が大幅に中和されて指
示薬の色の転換するに至る。Add one stick of bean paste to the survey area M+, and at that time, use the indicator used in the culture medium of the collection culture system. Add sodium hydroxide diluted solution from Narita to the sample, and add A and E to neutralize the acid to a large extent, leading to a change in the color of the indicator.
採取培案装置の吸入枝管を可食すべき試料に浸し培養基
容器に接紗する。容器内の負圧が作用して調査液体が1
廿養基中へ流入する。Dip the suction branch of the collection culture device into the edible sample and attach it to the culture medium container. The negative pressure inside the container acts and the liquid to be investigated becomes 1
Flows into the nutritional base.
1投柚ずみの装置は慎重に揺動させて試料と培養基とが
確実によく混合されるようにする。引続いて接1市ずみ
の装置を垂直の姿勢とし残液がある場合にはン岡をりυ
つた)=に開放の吸べ枝盲ンFに同けたまま、戚1:d
の圃・1♀ン施こした仝の載−の伐止すみ訂凝容器上i
l?l:1遅さ、その隙に枝dは綿栓を責かせる。Carefully rock the citrus device to ensure that the sample and culture medium are well mixed. Next, place the wet device in a vertical position, and if there is any remaining liquid, remove the
ivy) = with the open sucker branch blind F, relative 1: d
On the top of the coagulation container in the cut-off corner of the field where 1♀toning was carried out
l? l: 1 slow, branch d blames the cotton plug.
装置全体を架台に取付0Jて30Cにおい又り11化さ
ぜる。Attach the entire device to a stand and warm it to 30C and 11C.
採取培養システムとしては第3図に示した説の不発明の
装置が役立ら、これでは排出枝管か改変してあり、υ1
1出する培養&9gl9.困助S濁故のための校正ずみ
目盛つき肘凰芥器取句の可能1午がある。毎日液状培養
基の 濁、試料採散培養装置Nから01斥される培養基
病原菌懸濁液の容積及び指示薬色調の変化を検査する。As a collection and culture system, the uninvented device shown in Figure 3 is useful, and in this case the discharge branch pipe has been modified, and υ1
1 Culture & 9gl9. There is a possibility of a calibrated scale for troubleshooting problems. Every day, the liquid culture medium is inspected for turbidity, the volume of the culture medium pathogen suspension removed from sample collection and culture device N, and changes in the color tone of the indicator.
調査結果を第1図に示す。The survey results are shown in Figure 1.
レモネード原液中の醗酵性及び酸生成性南機体の同時検
出の場合、刺青に先立って原液を滅菌水で希釈し、上記
のLかたと同様にし″C滅昆の指示薬を加え、PH値を
滅菌の水酸化ナトIJウム規定溶液により調整する。后
続の諸段階は上記のとおり行なわれる。病原菌負荷御j
定にあたっては希釈倍数を考#i、″′fべきである。For the simultaneous detection of fermentable and acidogenic sulfur in a lemonade stock solution, dilute the stock solution with sterile water prior to tattooing, add a "C sterilization indicator," and sterilize the pH value. The following steps are carried out as described above. Pathogen load control
In determining the dilution ratio, the dilution factor should be #i, ″′f.
調査結果を第2図に示す。The survey results are shown in Figure 2.
大腸固型の病原菌の俟出の場合は採取培養システムに特
殊な培養基たとえばゲンチアナ−胆汁ラリドース/行欣
を71?)た丁。試料の準備ならびに自責−事べき散体
の充填及び眉続の病原菌の瞬化もE記と同様に行なわれ
る。In the case of a large amount of pathogenic bacteria in the large intestine, use a special culture medium in the collection culture system, such as Gentian bile laridose / 71? ) Ta Ding. Preparation of the sample, filling of the necessary dispersion, and blinking of the pathogenic bacteria in the eyebrows are also carried out in the same manner as in Section E.
ヒストンにより又は系内の負圧により正確に定めた童7
ことえは1又は5−の試料を試料採取培養装置内へ吸入
する。試料の1トン化及び評価のノ5にガス発生はなし
で試料容積l−又は5ゴにおいてr陰注大腸菌型到菌を
示″′f結果となる。Precisely determined by histones or by negative pressure in the system7
Kotoe inhales the 1 or 5- sample into the sample collection and culture device. After converting the sample to 1 ton and evaluating No. 5, no gas was generated, and the result showed that E. coli type bacteria had arrived in the sample volume 1 or 5.
汚染物賀検出用装置戊は公知のとおり破壊壊7を介して
折取口」龍のガラス元端2及び挿管3を備えた培養容器
からなる。この挿管3は同時に調査−すべき成体試料の
ための吸入枝管としてまたカス発生の除に排斥される培
養基病原菌1敞濁液のための鹿、出4支管としても形1
.& L ”Cある。望ましくは直径が3間未イ両であ
り培養層に成田の栓13を超えて目盛つきd[蹴容器4
内に突入して保持される。The device for detecting contaminants consists of a culture vessel equipped with a glass end 2 and an intubation tube 3, which can be broken off through a breakage port 7, as is known in the art. This intubation tube 3 can be used simultaneously as an inhalation branch tube for adult samples to be investigated, and also as an outlet tube for a culture medium pathogen 1 suspension to be rejected to eliminate the generation of scum.
.. & L "C. Preferably, the diameter is 3 mm, and the culture layer has a scale beyond the Narita stopper 13 [kick container 4
Thrust inside and held.
培養容器l内には液状培養基5がありこの容器1外には
一つ又はいくつかの固形培養基6のためのカラス容器I
Oが配置1 してめる。この゛容器10は望ましくは1
ス分L″′Cある′容器とし工具室の又はガス充填の室
9のかたわらにありかつ固形培養基6のための支持部H
が設けである。il/p化Bれた固形培養基6の病原菌
ル鞠査のため試料採取はカラス容器11を破壊壌8のと
ころで開いた后にこILから行なわれる。Inside the culture vessel I there is a liquid culture medium 5 and outside this vessel 1 there is a glass vessel I for one or several solid culture mediums 6.
O is placed 1. This container 10 is preferably 1
space L'''C' as a container next to the tool room or gas-filled chamber 9 and supporting part H for the solid culture medium 6.
is the provision. Samples are collected from the IL after the container 11 is opened at the destruction medium 8 in order to examine the pathogenic bacteria in the solid culture medium 6 which has been subjected to IL/P conversion.
第1図は清涼飲料中の病原菌のガス発生につい℃のグラ
フ
第2図はレモネード原欣中のプ内1京閑0ガス発生につ
いてのグラフ
第3因は本発明による装置でめつ℃さまさまな用途のも
の
a)液状培養基用のヒストンつき開放容器システム
b)液状及び固形培養基用のヒストンつき開放容器シス
テム
第4図は11】」じく
即成状培養基用の閉鎖容器システム
b)散状及び同形培養糸用閉鎖芥器システムを示−10
1、・・・培養容器
2、・・・折取可能のガラス尖端
3、・・・挿管
4、・・・目盛つき81暇容R診
5、・・・故状櫓養基
6、・・・固jし培#基
7、・・・容器l用の破壊譲
8、・・・ガラス容器1に用の破壊嬢
9、・・・真窒(カス充填)室
10、・・・同形、l@養基6 Jilのカラス容器■
、・・・支荷都
稔、…ピストン
B、・・・滅菌の栓
イベ(オi7−+丁、!−イう二 上角)−籐図面の浄
告−(内存に変更なし)
第 ll!!
1 2 3 4 υ
第 35!1
a l)
#E 4 図
a b
第1頁の続き
0発 明 者 アルツール・クンッ
ドイツ民主共和国8301ブルクハ
ルツヴアルデヌンメル60
@発 明 者 ブリギッチ・シュドローペルドイツ民主
共和国8046ドレスデ
ン・ラテナー・シュトラーセ59
手続補正書
昭和57年12月17日
特許庁長官 若杉 和夫 殿
1、事件の表示
昭和57年特願第 136799号
2、発明の名称
汚染物質検出の方法及び装置
3、補正をする者
事件との関係 特許出願人
住 所 ドイツ民生共和国 642 ノイハウス エ
ル ヴエゲエ ゾンネペルガー シュトラーセ 73名
称 フオルクスアイグナー ベトリープ ファルマ
グラスヴエルク ノイハウス
4、代理人
住 所 東京都中央区日本橋2−6−3 斉藤特許
ビル6、補正の内容 別紙の通り。Figure 1 is a graph of gas generation from pathogenic bacteria in soft drinks in degrees Celsius. Figure 2 is a graph of gas generation in lemonade. Applications a) Open container system with histones for liquid culture media b) Open container system with histones for liquid and solid culture media and a closure system for isomorphic culture threads-10 1,...Culture vessel 2,...Breakable glass tip 3,...Intubation 4,...Graduated 81 time R examination 5 , . . . Destroyed container 1 9, . . . Nitrogen (scum filling) chamber 10,... Same shape, l@Nursing base 6 Jil's crow container■
,...Shipping To Minoru,...Piston B,...Sterilization stopper (Oi7-+Dou,!-Iu2 upper corner) -Review of the rattan drawing-(No change in contents) No. 11 ! ! 1 2 3 4 υ 35!1 a l) #E 4 Figure a b Continued from page 1 0 Inventor Artur Kunt German Democratic Republic 8301 Burgharzwaldenummel 60 @ Inventor Brigitsch Sudropel German Democratic Republic 8046 Dresden-Latener Strasse 59 Procedural Amendment December 17, 1980 Director General of the Patent Office Kazuo Wakasugi 1, Indication of the Case Patent Application No. 136799 of 1981 2, Name of the Invention Method for detecting pollutants and Apparatus 3, relationship to the case of the person making the amendment Patent applicant address: German Democratic Republic 642 Neuhaus el Veeghe Sonneperger Straße 73 Name: Volksaigner Betrib Pharmagraswerk Neuhaus 4, Agent address: 2- Nihonbashi, Chuo-ku, Tokyo 6-3 Saito Patent Building 6, contents of amendments As shown in the attached sheet.
Claims (1)
菌含有の液体又は調査の目的で公知の方法に従って病原
菌を導入する液体を複合試料採取培養装置内において選
ばれた城の培養基と混合するものにおいて、引続いての
培養基病原菌)セ濁液の培養は試料採取培養装置の下方
μ目数の排出枝管上方で行なわれ、培養中に調査微生物
によるカス発生の場合は同価の111す定aJ能Mの培
養基病原菌!懸濁液がMC出しその際指示系含有の培養
基の濁度及び色彩変化と関連して同時にアナログ数値を
もって汚染微生物の病原菌含有量、ガス発生、酸酢活性
及び酸生成についての定量的報告が行なわれることを特
徴とする方法 2 培9容器の別個の物分に一つ又はいくつかの固形培
養基が41加的にもたらしてあり、調査丁べき液体の導
入店にこれら同形培養基上において調査病原園の東落菌
芝状成表き行なわれ、培饗后に顕微鏡倹食及び又はその
他の脚管ならびに微生物の繁殖のための試料を採取する
ことを特徴とする/l!肝請求の範囲第1JJ4記載の
方法 3 培養容器、折取可能のガラス鋏光端、挿管及び破壊
様ならびに液状及び固形培養基を倫えた複合試料採取培
養装置からなる汚染物置検出装置において、挿管(3)
は同時に、副食丁べき液体のための吸入枝抽′として4
だガス発生の際に排 される培養基病原菌懸濁/汐のた
めの排出宜としても形成しており、頃ましくは1貝径が
3門未鈎であり、滅丙の程d4を超えて目盛つきit
diす容器(4)内に突入して保持してめることをtP
f徴とする装置。 4 液状培養基(5)のための培害容器(すの外部に一
つ又はいくつかの固形培養基(6〕のための、−(まし
くは区分さハた容器としてのカラス容器Qlかに’Eの
又はガス充填の室(9)のかたわらに配醸してあり筐だ
固形培養基(6)用支持部αpかi′化けであることを
特徴とする特ii’f g#求の範囲第3墳Ht戦の装
置唯[Scope of Claims] 1. A method for the detection of contaminants in the food industry, comprising: a method for detecting contaminants in the food industry, comprising: processing a liquid containing pathogens or a liquid into which pathogens are introduced for investigation purposes according to known methods; For those mixed with a culture medium, the subsequent culture of the culture medium (pathogenic bacteria) suspension is carried out above the discharge branch pipe of the lower micrometer of the sample collection and culture device. Culture medium pathogenic bacteria with a constant aJ capacity of 111! When the suspension is released into the MC, a quantitative report is made on the pathogen content of the contaminant microorganisms, gas evolution, acid-acetic acid activity and acid production with simultaneous analog numerical values in conjunction with the turbidity and color changes of the culture medium containing the indicator system. Method 2, characterized in that one or several solid culture media are additionally provided in separate portions of 9 culture vessels, and the investigation pathogen culture is carried out on these homogeneous culture media into the introduction store of the liquid to be investigated. It is characterized by carrying out the cultivation of the eastern bacterial turf and collecting samples for microscopic feeding and/or other leg canal and microbial propagation after culturing. Liver Claims No. 1 JJ4 Method 3 In a contaminant detection device consisting of a culture vessel, a breakable glass scissor light end, an intubation and destruction mode, and a composite sample collection and culture device equipped with liquid and solid culture media, intubation (3 )
At the same time, it also serves as a suction branch for the liquid that should be used as a side dish.
It is also formed as a drain for the suspension of pathogenic bacteria in the culture medium that is discharged when gas is generated, and it is best to have three unhooked shells in one shell, and it is rare that the diameter of one shell exceeds d4. It with scale
Plunge into the disu container (4) and hold it.
A device that makes f-signs. 4 Cultivation vessels for liquid culture medium (5) (on the outside of the container for one or several solid culture mediums (6) - (or as divided vessels Ql) Part 3 of the range of interest, characterized in that the supporting part αp or i' of the solid culture medium (6) in the case is disposed next to the gas-filled chamber (9). Mound Ht Battle Device Yui
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13679982A JPS5928495A (en) | 1982-08-05 | 1982-08-05 | Method and apparatus for detecting pollutants |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13679982A JPS5928495A (en) | 1982-08-05 | 1982-08-05 | Method and apparatus for detecting pollutants |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5928495A true JPS5928495A (en) | 1984-02-15 |
Family
ID=15183784
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13679982A Pending JPS5928495A (en) | 1982-08-05 | 1982-08-05 | Method and apparatus for detecting pollutants |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5928495A (en) |
-
1982
- 1982-08-05 JP JP13679982A patent/JPS5928495A/en active Pending
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