JPS5928495A - Method and apparatus for detecting pollutants - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION A method for the detection of non-inventive contamination '+th ＃＋It is useful for both castle exploration and culturing of microorganisms found in samples.

For detection of contaminants, product or equipment equipment q1
There are well-known methods that can report on the characteristics of negative values for pathogenicity of a part. However, as a rule, °C detection is carried out in several work steps.

The collection of a specific volume of a sample from a liquid or suspension in the laboratory is carried out using appropriate equipment (e.g. a bed) in order to culture it in solid and/or liquid culture medium without cutting the corresponding compartments of the sample. Let's do it from now on. The container can be, for example, a beverage bottle or a transport container, and is filled with the sample outside the laboratory under sterile conditions. The sample size (1'L) obtained by microscopic imaging is only 0] in the case of commercial contamination. There is a risk of cross-contamination due to the number of containers handled and contact with nitrogen.

For example, in order to test the shelf life of bedbugs, it takes a woodcutter to find contaminants even in a very small number.

For this purpose, a large volume of sample is separated by membrane and brought onto a solid culture medium.

However, when it comes to the L' = feeding method, weakly diseased meat grows slower on a solid culture medium than in a liquid culture medium, so a satisfactory result can only be obtained after incubation for at least 3 to 4 days. I cannot accept 4. The liquid culture medium also applies to the well-known lithographic culture of Kotsuho.

The staining of microorganisms separated by membrane filtration and their subsequent incubation and characterization are only possible in a well-equipped J3R and require corresponding qualifications. In addition, direct n [0 for determining microbial life and evening
” Limit your potential.

In addition to the degree of contamination, the major drawback of the US method is that
3) Contamination (various properties of living organisms (gas generation, fermentation activity,
Acid production and anaerobic VFF growth are also difficult to measure.

In the food industry and similar industrial packaging, the ability to produce gases and acids in aerobic conditions and to grow in aerobic conditions is recognized for pathogens that are on the nails.
+h-4.

(In the previous method, the gas generation must be detected qualitatively.) Or, it is better to use a separate f1^1 culturing device (for example, it is necessary to set the The method used in the United States is to place equipment and culture media in the laboratory. [Kabigai] 0 In addition to 1.Xq-, a system for blood collection and processing of blood samples is known.

According to No. 3536061 and No. 2F335101, West German Duke 1'i [l 'F) Ill'
Sample J to the 41μ positive system that was brokered by <! :! Carrier'
For example, if a small sample tube filled with nitrogen is connected to a small sample tube filled with nitrogen, it is possible to use a full document container and a small sample tube filled with nitrogen.West German openness GiF Application No. 24
According to No. 11651, there is a pressure-sensitive, gas-impermeable obstruction inside the blood collection system! It was shown that scum was generated through γ, and the possibility that the gas analyzer was loose was raised.

The blood sampling and culturing system for diagnosing sepsis described in West German WF Application No. 3012056 is capable of simultaneous culturing on liquid and solid culture media, and the blood is collected through the sampling system in a fixed line. The shallow culture 4vc is then inoculated into the solid culture medium as well when it is aspirated into the nitrogen-filled or sludge-filled vessel of the culture system.

P) Visible is the oxidized brother that indicates mulberry, which is controlled by l, and is combined with growth.

Decubation is performed with the system placed obliquely, while the collection cannula remains closed with a 1-family plastic stopper. However, this system is not applicable to the food industry.

The object of the invention is to develop a method and apparatus for the food industry and food industry field of products and realistic microbiological investigation, which rarely have the drawbacks of the traditional P4 determination method, thereby making it possible to The degree of contamination and the various characteristics of pollutants/l'η characteristics 1i3I speed ↓1
The purpose of the detection of the 9th cup is to create something that can also be used as a disposable container.

The present invention has the following features:
``At times, we have developed a method to reduce the time-consuming burden of work by integrating all of the essential aspects of sample collection and culture into one prefecture using liquid and/or isomorphic culture. i% of contaminants in the liquid or in the liquid according to known methods.
? 4? This is the basis for the development of a device that allows the detection of microorganisms caused by female microorganisms to be 51 years old.

The method according to the invention is particularly suitable for the detection of bacteria, yeasts and filamentous substances, in which the liquid sample is electrostatically charged under reduced pressure and/or filled with a small gas (e.g. CO,...
Abdominal sampling of Nt) is aspirated into a culture vessel, whereupon it is mixed with a liquid culture medium.

Separate compartments of this device can contain one or several solid culture media at °C, so that the sample mixed with the slurry culture medium can be mixed simultaneously with the liquid and solid culture media (1 piece to 100 °C).
^1) can be attached to Germany.

Aerial organisms are cultured in a special rectangular warm environment by placing the container straight on the wall. Acid production ability is influenced by pH (F
I show 4 systems, especially bromcresol green (3', 5'
, +1', 5', due to discoloration of tetrabrom-cresolsulfonphthalein or bromophenol (tetrabromophenol-nurphonphthalein). According to the present invention, this method prevents the production of starving proboscis by fixing the container in a vertical position on a suitable pedestal with the priest facing F, and adding a sufficient volume of culture medium microorganism suspension to prevent the production of scum. The % sign is detected by being expelled from the container and placed in a calibrated, 1-2 or sterile, canalicular tube closed with a sterile cotton stopper.

In addition, the method according to the invention is characterized in that various properties of contaminating microorganisms, such as pathogenic microorganisms, scum generation, fermentation activity or acid production, can be determined at the same time.
(-) Therefore, testing of the fermentation activity of the production yeast used can also be carried out according to this method.

For a separate tube-shaped receptacle, the culture layer is made of a temporary and/or solid culture medium, and the sample is taken - 3''. It is also formed as an inlet branch pipe for the liquid to be cultivated, and has a diameter of 311 nm. The sterile stopper is less than 6 degrees Celsius with a scale of M.
111 It is a 'II characteristic that the object is plunged into a fixed container and held there. In addition, this prefectural government has one or several
The container for the liquid culture medium is preferably separated 7' from the outside of the container for the liquid culture medium! (It is characterized by the fact that a support section for the IJ type culture medium is installed next to the bulge or gas-filled chamber.

An advantage of the method and device according to the invention is that the invention allows simultaneous detection of gas-producing and acid-producing microorganisms. In contrast to the proposed known processes, the process is therefore advantageously applicable in the fermentation and beverage industry and in similar industrial fields.

Compared to known devices, a separate gas sampling casing is not required.

This is because scum formation is indicated by the escape of the culture medium from the sampling branch.

Unlike known methods, when the fermentation is active and the mother is present, it is possible to sample both layers for true blue color during fermentation.

This system is suitable not only for detecting contaminating microorganisms but also for confirming the fermentation activity of production yeast.

The body for the 16J type culture medium is a rewiring to the outside of the device. After attaching the solid culture medium, the VC is grown into a culture medium. Therefore, some Rakusei relatives can be congratulated immediately. Another advantage of this device is that compared to conventional methods, the culture medium to be tested is collected directly, for example by puncturing the membrane for sample collection purposes, so contamination of the sample rarely occurs. It's in the absence of it.

The method and apparatus according to the present invention will be explained below with reference to actual measurements.

(In the case of simultaneous detection of 11ζl fermentable and acid-forming minerals in a soft drink, the beverage should be decarboxylated prior to the contaminant investigation, as is generally done in biochemical diets. If I don't have it, it's 7.C.

Add one stick of bean paste to the survey area M+, and at that time, use the indicator used in the culture medium of the collection culture system. Add sodium hydroxide diluted solution from Narita to the sample, and add A and E to neutralize the acid to a large extent, leading to a change in the color of the indicator.

Dip the suction branch of the collection culture device into the edible sample and attach it to the culture medium container. The negative pressure inside the container acts and the liquid to be investigated becomes 1
Flows into the nutritional base.

Carefully rock the citrus device to ensure that the sample and culture medium are well mixed. Next, place the wet device in a vertical position, and if there is any remaining liquid, remove the
ivy) = with the open sucker branch blind F, relative 1: d
On the top of the coagulation container in the cut-off corner of the field where 1♀toning was carried out
l? l: 1 slow, branch d blames the cotton plug.

Attach the entire device to a stand and warm it to 30C and 11C.

As a collection and culture system, the uninvented device shown in Figure 3 is useful, and in this case the discharge branch pipe has been modified, and υ1
1 Culture & 9gl9. There is a possibility of a calibrated scale for troubleshooting problems. Every day, the liquid culture medium is inspected for turbidity, the volume of the culture medium pathogen suspension removed from sample collection and culture device N, and changes in the color tone of the indicator.

The survey results are shown in Figure 1.

For the simultaneous detection of fermentable and acidogenic sulfur in a lemonade stock solution, dilute the stock solution with sterile water prior to tattooing, add a "C sterilization indicator," and sterilize the pH value. The following steps are carried out as described above. Pathogen load control
In determining the dilution ratio, the dilution factor should be #i, ″′f.

The survey results are shown in Figure 2.

In the case of a large amount of pathogenic bacteria in the large intestine, use a special culture medium in the collection culture system, such as Gentian bile laridose / 71? ) Ta Ding. Preparation of the sample, filling of the necessary dispersion, and blinking of the pathogenic bacteria in the eyebrows are also carried out in the same manner as in Section E.

Precisely determined by histones or by negative pressure in the system7
Kotoe inhales the 1 or 5- sample into the sample collection and culture device. After converting the sample to 1 ton and evaluating No. 5, no gas was generated, and the result showed that E. coli type bacteria had arrived in the sample volume 1 or 5.

The device for detecting contaminants consists of a culture vessel equipped with a glass end 2 and an intubation tube 3, which can be broken off through a breakage port 7, as is known in the art. This intubation tube 3 can be used simultaneously as an inhalation branch tube for adult samples to be investigated, and also as an outlet tube for a culture medium pathogen 1 suspension to be rejected to eliminate the generation of scum.
．． & L "C. Preferably, the diameter is 3 mm, and the culture layer has a scale beyond the Narita stopper 13 [kick container 4
Thrust inside and held.

Inside the culture vessel I there is a liquid culture medium 5 and outside this vessel 1 there is a glass vessel I for one or several solid culture mediums 6.
O is placed 1. This container 10 is preferably 1
space L'''C' as a container next to the tool room or gas-filled chamber 9 and supporting part H for the solid culture medium 6.
is the provision. Samples are collected from the IL after the container 11 is opened at the destruction medium 8 in order to examine the pathogenic bacteria in the solid culture medium 6 which has been subjected to IL/P conversion.

[Brief explanation of drawings]

Figure 1 is a graph of gas generation from pathogenic bacteria in soft drinks in degrees Celsius. Figure 2 is a graph of gas generation in lemonade. Applications a) Open container system with histones for liquid culture media b) Open container system with histones for liquid and solid culture media and a closure system for isomorphic culture threads-10 1,...Culture vessel 2,...Breakable glass tip 3,...Intubation 4,...Graduated 81 time R examination 5 , . . . Destroyed container 1 9, . . . Nitrogen (scum filling) chamber 10,... Same shape, l@Nursing base 6 Jil's crow container■
,...Shipping To Minoru,...Piston B,...Sterilization stopper (Oi7-+Dou,!-Iu2 upper corner) -Review of the rattan drawing-(No change in contents) No. 11 ! ! 1 2 3 4 υ 35!1 a l) #E 4 Figure a b Continued from page 1 0 Inventor Artur Kunt German Democratic Republic 8301 Burgharzwaldenummel 60 @ Inventor Brigitsch Sudropel German Democratic Republic 8046 Dresden-Latener Strasse 59 Procedural Amendment December 17, 1980 Director General of the Patent Office Kazuo Wakasugi 1, Indication of the Case Patent Application No. 136799 of 1981 2, Name of the Invention Method for detecting pollutants and Apparatus 3, relationship to the case of the person making the amendment Patent applicant address: German Democratic Republic 642 Neuhaus el Veeghe Sonneperger Straße 73 Name: Volksaigner Betrib Pharmagraswerk Neuhaus 4, Agent address: 2- Nihonbashi, Chuo-ku, Tokyo 6-3 Saito Patent Building 6, contents of amendments As shown in the attached sheet.

Claims (1)

[Scope of Claims] 1. A method for the detection of contaminants in the food industry, comprising: a method for detecting contaminants in the food industry, comprising: processing a liquid containing pathogens or a liquid into which pathogens are introduced for investigation purposes according to known methods; For those mixed with a culture medium, the subsequent culture of the culture medium (pathogenic bacteria) suspension is carried out above the discharge branch pipe of the lower micrometer of the sample collection and culture device. Culture medium pathogenic bacteria with a constant aJ capacity of 111! When the suspension is released into the MC, a quantitative report is made on the pathogen content of the contaminant microorganisms, gas evolution, acid-acetic acid activity and acid production with simultaneous analog numerical values in conjunction with the turbidity and color changes of the culture medium containing the indicator system. Method 2, characterized in that one or several solid culture media are additionally provided in separate portions of 9 culture vessels, and the investigation pathogen culture is carried out on these homogeneous culture media into the introduction store of the liquid to be investigated. It is characterized by carrying out the cultivation of the eastern bacterial turf and collecting samples for microscopic feeding and/or other leg canal and microbial propagation after culturing. Liver Claims No. 1 JJ4 Method 3 In a contaminant detection device consisting of a culture vessel, a breakable glass scissor light end, an intubation and destruction mode, and a composite sample collection and culture device equipped with liquid and solid culture media, intubation (3 )
At the same time, it also serves as a suction branch for the liquid that should be used as a side dish.
It is also formed as a drain for the suspension of pathogenic bacteria in the culture medium that is discharged when gas is generated, and it is best to have three unhooked shells in one shell, and it is rare that the diameter of one shell exceeds d4. It with scale
Plunge into the disu container (4) and hold it.
A device that makes f-signs. 4 Cultivation vessels for liquid culture medium (5) (on the outside of the container for one or several solid culture mediums (6) - (or as divided vessels Ql) Part 3 of the range of interest, characterized in that the supporting part αp or i' of the solid culture medium (6) in the case is disposed next to the gas-filled chamber (9). Mound Ht Battle Device Yui