JPS5922620A - Glucose filter - Google Patents

Glucose filter

Info

Publication number
JPS5922620A
JPS5922620A JP57131655A JP13165582A JPS5922620A JP S5922620 A JPS5922620 A JP S5922620A JP 57131655 A JP57131655 A JP 57131655A JP 13165582 A JP13165582 A JP 13165582A JP S5922620 A JPS5922620 A JP S5922620A
Authority
JP
Japan
Prior art keywords
glucose
membrane
holes
concentration
dissolved oxygen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP57131655A
Other languages
Japanese (ja)
Inventor
Kaname Ito
要 伊藤
Yoshio Kawai
河合　義雄
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kureha Corp
Original Assignee
Kureha Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kureha Corp filed Critical Kureha Corp
Priority to JP57131655A priority Critical patent/JPS5922620A/en
Priority to FR8312327A priority patent/FR2530962A1/en
Priority to GB08320257A priority patent/GB2124545A/en
Priority to DE19833327096 priority patent/DE3327096A1/en
Priority to DD25349783A priority patent/DD211636A5/en
Publication of JPS5922620A publication Critical patent/JPS5922620A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Filtering Materials (AREA)

Abstract

PURPOSE:To improve a glucose sensor capable of responsing to high-concentration glucose, by developing a glucose-restricting permeable membrane having a specified shape, restricting the permeation of glucose and allowing that of dissolved oxygen. CONSTITUTION:The membrane in this invention is hydrophobic and is permeable to dissolved oxygen, and the membranes made of polyperfluoroethylene, polyethylene, and polypropylene, etc. having 25-60% porosity are used, and the hole diameter is in a range of 10-100mu average diameter, and the number of holes are 10-1,000/cm<3>. The number of holes are preferably made less in the case of measuring the high-concentration glucose and more for the low-concentration glucose. The gap between fine holes is required to be as uniform as possible in respect of measuring accuracy and responsing time. The shape of hole is not specially limited but the perfect roundness is preferable.

Description

【発明の詳細な説明】 本発明は酵素電極型グルコースセンサーに供するグルコ
ース制限透過膜に係る。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a glucose-limiting permeable membrane used in an enzyme electrode glucose sensor.

グルコースオキシターゼを固定した膜を使用し、グルコ
ースを溶存酸素で酸化することによシ溶液中のグルコー
ス量を測定するグルコースセンサーは良く知られている
う その原理は下記反応を利用するもので、溶存酸素濃度或
いは過酸化水素濃度の測定から間接的にグルコース濃度
を知るものである。The glucose sensor uses a membrane immobilized with glucose oxidase to measure the amount of glucose in a solution by oxidizing glucose with dissolved oxygen. Glucose concentration is indirectly known from the measurement of oxygen concentration or hydrogen peroxide concentration.

グルコースオキシターゼ グルコース十へ土山０−−→グルコンｓ＋ｉ４ｏ。glucose oxidase Glucose 1 to Tsuchiyama 0--→glucon s+i4o.

上記反応式で明らかな如く、グルコース濃度の測定限界
は醇存酸素量によって決まってしまい、固定化酵素膜を
溶存酸素センサーのテフロン膜上に密着させた従来のグ
ルコースセンサーでは高グルコース濃度側での識別が困
難であった。この為。As is clear from the above reaction equation, the measurement limit of glucose concentration is determined by the amount of dissolved oxygen, and the conventional glucose sensor, in which an immobilized enzyme membrane is tightly attached to the Teflon membrane of the dissolved oxygen sensor, cannot measure the glucose concentration at high glucose concentrations. It was difficult to identify. For this reason.

糖尿病、その他の原因にょシ血糖が増加した検体（血液
）中のグルコース量を測定する場合、従来のグルコース
センサーでは採血して酸素を補給するとか、血液を希釈
するなどの処理を要し、実用上で難点があった。When measuring the amount of glucose in a sample (blood) with increased blood sugar levels due to diabetes or other causes, conventional glucose sensors require processing such as collecting blood to supply oxygen or diluting the blood, making it impractical. There was a problem at the top.

従って、高グルコース濃度に応答し、それを識別できる
グルコースセンサーの開発が望まれるところである。Therefore, it is desired to develop a glucose sensor that can respond to and identify high glucose concentrations.

本発明者らは、グルコースの透過を制限し且っ溶存酸素
の透過を妨げない特殊な形状のグルコース制限透過膜の
開発によル、上記殊題を解決したものである。The present inventors have solved the above-mentioned special problem by developing a glucose-limiting permeable membrane with a special shape that limits the permeation of glucose but does not hinder the permeation of dissolved oxygen.

本発明のグルコース制限透過ｇ（以下本発明の膜と称す
る）を従来のグルコースセンサーに組み込むことで、高
グルコース濃度の被検液であっても希釈或いは特別の処
理を施すことなく直接測定を可能とするものである。By incorporating the glucose-restricted permeation g of the present invention (hereinafter referred to as the membrane of the present invention) into a conventional glucose sensor, it is possible to directly measure a test solution with a high glucose concentration without dilution or special treatment. That is.

本発明の膜はＩＭ＆　１０〜１００μの細孔をｌａｌ当
如１０ヶ〜１００００ケ有する疎水性の膜よシなる。The membrane of the present invention is a hydrophobic membrane having approximately 10 to 10,000 pores of 10 to 100 μm.

本発明に係る膜材は疎水性且つ溶存＃ｔＸを透過するも
のであれば良く１例えば空孔率２５〜６０％を有する（
≠フロン、ポリエチレン、ポリプロピレン、ポリフッ化
ビニリデン製の膜を例示する仁とができる。The membrane material according to the present invention may be any material as long as it is hydrophobic and permeable to dissolved #tX. For example, it has a porosity of 25 to 60% (
≠Membranes made of fluorocarbons, polyethylene, polypropylene, and polyvinylidene fluoride are examples.

孔径は平均直径１０〜１００μの範囲内にあれば良く、
膜の材質、厚さ或いは被検体のグルコース濃度範囲等に
よって任意に選択される。１０μ以下では殆んど通液が
起らず、また、１００μ以上では目的とするグルコース
の透過量を制限できない。The pore size should be within the range of average diameter 10 to 100μ,
It is arbitrarily selected depending on the material and thickness of the membrane, the glucose concentration range of the subject, etc. If it is less than 10μ, hardly any liquid passes through it, and if it is more than 100μ, the desired amount of glucose permeation cannot be restricted.

孔形は真円が好ましいが、特に限定されない。The hole shape is preferably a perfect circle, but is not particularly limited.

卸１孔の数は１ｃｒ７を尚シ１０ヶ〜１００００ケであ
１高グルコース濃度の測定においては少なく、低グルコ
ース濃度の測定では多くすれば良い。また、細孔の間隔
は測定精度、応答時間の点で出来るだけ均等が望ましい
。The number of holes per hole is 10 to 10,000 per hole, which is small for measuring high glucose concentrations, and large for measuring low glucose concentrations. Further, it is desirable that the intervals between the pores be as uniform as possible in terms of measurement accuracy and response time.

孔径及び細孔の数を任意に選ぶことよ＃）、グルコース
の測定濃度範囲を調節することができる。By arbitrarily selecting the pore size and number of pores, the measurement concentration range of glucose can be adjusted.

本発明の膜は本来酸素透過性の膜であり、それにあけた
細孔を通して更にグルコースを部分的な移動しうる性質
を有する。従って１本発明の膜をクラーク型又はその変
形型酸素電極を用いた公知の酵素電極型グルー−スセン
サーの固定化酵累膜の前面に設置すれば、被検体を希釈
することなく、寿命、応答最大血糖値等を数台した高性
能グルコースセンサーとなシ得る。なお、センサーに組
み込む場合、本発明の膜を２枚以上重ねて又は貼シ合せ
て使用し得る。The membrane of the present invention is essentially an oxygen-permeable membrane, and has the property of further allowing partial movement of glucose through the pores formed therein. Therefore, if the membrane of the present invention is installed in front of the immobilized fermentation membrane of a known enzyme electrode type glucose sensor using a Clark type oxygen electrode or its modified oxygen electrode, the lifespan can be increased without diluting the analyte. It can be used as a high-performance glucose sensor that measures the response maximum blood sugar level, etc. In addition, when incorporating into a sensor, two or more membranes of the present invention may be stacked or laminated together.

以下、実施例をもって本発明を詳述する。Hereinafter, the present invention will be explained in detail with reference to Examples.

〔実施例〕〔Example〕

クラーク型酸素電極を用いた公知の？ルコースセンサー
の前に本発明の膜を取付けた時の応答性を調べた。Is there a known method using a Clark type oxygen electrode? The response when the membrane of the present invention was attached in front of the lucose sensor was investigated.

本発明の膜はジュラガード魔２５０１　（ポリプロピレ
ン社製）に５０〜１００μの穴を１目ピツチ及び０、５
　ｍピッチであけたものを使用した。なお、比較例は本
発明の膜を用いない従来のグルコースセンサーである。The membrane of the present invention has 50 to 100μ holes in DURAGUARD MA2501 (manufactured by Polypropylene Co., Ltd.) with one pitch and 0,5 holes.
The ones with m pitches were used. Note that the comparative example is a conventional glucose sensor that does not use the membrane of the present invention.

その結果を添附図面に示す。The results are shown in the attached drawings.

添附図面から明らかな如く１本発明のｍめ使用によシグ
ルコース濃度ＴｏｏＩＩＶｄｔ以上まで測定が可能とな
った。As is clear from the accompanying drawings, by using the method of the present invention, it has become possible to measure siglucose concentrations up to TooIIVdt or higher.

【図面の簡単な説明】[Brief explanation of drawings]

添附図面は、グルコース濃度に対するセンサー出力の関
係を示す図である。The accompanying drawing is a diagram showing the relationship between sensor output and glucose concentration.

Claims (1)

【特許請求の範囲】[Claims] （１）　　疎水性の膜に直径１０〜１００μの孔を１０
ケ〜１ｏｏｏｏ　％（あけてなるグルコース制限透過膜
。(1) Add 10 pores with a diameter of 10 to 100μ to a hydrophobic membrane.
K ~ 1oooo% (Glucose restriction permeable membrane formed by opening.
JP57131655A 1982-07-28 1982-07-28 Glucose filter Pending JPS5922620A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP57131655A JPS5922620A (en) 1982-07-28 1982-07-28 Glucose filter
FR8312327A FR2530962A1 (en) 1982-07-28 1983-07-26 MEMBRANE FOR CONTROLLING THE PERMEATION OF GLUCOSE THROUGH IT
GB08320257A GB2124545A (en) 1982-07-28 1983-07-27 Membrane fro regulating permeation of glucose therethrough
DE19833327096 DE3327096A1 (en) 1982-07-28 1983-07-27 MEMBRANE WITH CONTROLLED GLUCOSE PERMEATION
DD25349783A DD211636A5 (en) 1982-07-28 1983-07-28 MEMBRANE FOR REGULATING THE PERMEATION OF GLUCOSE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP57131655A JPS5922620A (en) 1982-07-28 1982-07-28 Glucose filter

Publications (1)

Publication Number Publication Date
JPS5922620A true JPS5922620A (en) 1984-02-04

Family

ID=15063124

Family Applications (1)

Application Number Title Priority Date Filing Date
JP57131655A Pending JPS5922620A (en) 1982-07-28 1982-07-28 Glucose filter

Country Status (5)

Country Link
JP (1) JPS5922620A (en)
DD (1) DD211636A5 (en)
DE (1) DE3327096A1 (en)
FR (1) FR2530962A1 (en)
GB (1) GB2124545A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6283648A (en) * 1985-10-08 1987-04-17 Daikin Ind Ltd Method for measuring concentration of glucose in solution
US6280587B1 (en) 1998-07-02 2001-08-28 Nec Corporation Enzyme electrode and a biosensor and a measuring apparatus therewith

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD297713A5 (en) * 1990-08-31 1992-01-16 Akademie Der Wissenschaften Der Ddr,Dd ENZYME MEMBRANE FOR DETERMINING HIGH SUBSTRATE CONCENTRATIONS WITH ENZYME SENSORS
DE4221848C2 (en) * 1992-07-03 2001-04-12 Eckhard Salzsieder Method and arrangement for automatic in situ calibration of intracorporeal glucose measuring devices

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1084079A (en) * 1964-11-30 Beckman Instruments Inc
US3591480A (en) * 1968-07-15 1971-07-06 Ibm Glucose measuring system
BE792378A (en) * 1971-12-07 1973-06-06 Rhone Poulenc Sa ORGANOSILICIC POLYMER MEMBRANE
SE396819B (en) * 1975-12-31 1977-10-03 Gambro Ab METHOD AND DEVICE FOR DETERMINING THE CONCENTRATION OF A LOW MOLECULAR ASSOCIATION IN A COMPLEX MEDIUM BY DIALYSIS
JPS5857205B2 (en) * 1976-02-02 1983-12-19 住友化学工業株式会社 Manufacturing method of semipermeable membrane
GB2053789B (en) * 1979-07-11 1982-12-08 Ca Minister Nat Defence Microporous protective sheet
JPS5627643A (en) * 1979-08-14 1981-03-18 Toshiba Corp Electrochemical measuring device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6283648A (en) * 1985-10-08 1987-04-17 Daikin Ind Ltd Method for measuring concentration of glucose in solution
JPH043822B2 (en) * 1985-10-08 1992-01-24
US6280587B1 (en) 1998-07-02 2001-08-28 Nec Corporation Enzyme electrode and a biosensor and a measuring apparatus therewith

Also Published As

Publication number Publication date
DE3327096A1 (en) 1984-02-02
GB8320257D0 (en) 1983-09-01
GB2124545A (en) 1984-02-22
FR2530962A1 (en) 1984-02-03
DD211636A5 (en) 1984-07-18

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