JPS59190397A - Ring type electrophoresis device - Google Patents

Abstract

PURPOSE:To perform linearly parallel migration of a living body in either position by forming both electrodes of an electrophoresis device into a closed ring having no end parts and forming the frames on both sides of an electrophoresis membrane into a cylindrical shape having the same sectional shape as the sectional shape of both electrodes. CONSTITUTION:Both electrodes 11, 12 of a closed type electrophoresis device are made respectively into a circular ring shape (or elliptical ring shape or polygonal ring shape) and both frames 31, 32 are made respectively into a cylindrical shape (or elliptical cylindrical shape or polygonal cylindrical shape). Then outside end parts do not exist in the electrodes 11, 12 and the frames 31, 32 on both sides of the electrophoresis membrane, and therefore the outside end parts do not exist on the phoresis membrane as well. The migrating condition of a bio- fluid is then made linear and is convienent for analysis of the migration image.

Description

[Detailed description of the invention] The present invention relates to a closed-form electrophoresis device.

Conventionally, there have been various types of electrophoresis devices in which biological fluids are separated by electrophoresis in a gel made of membrane-like starch or acrylamide, but in all of them, both electrodes are placed in parallel. It was shaped like a rod or a flat plate. However, when electrophoresis is performed on a plurality of living organisms using such an electrode, the electrophoresis situation becomes thicker and closer to the outside of the electrophoresis membrane as it moves from the top to the bottom, as shown in Figure 1. The shape of the biological fluid cannot be avoided, and as a result, subsequent observation of the biological fluid becomes troublesome.

The cause of this phenomenon is that there is a difference in temperature and humidity between the peripheral area of the electrophoretic membrane and the other areas, and this causes the descending biological fluid to drift laterally outward, or the temperature and humidity are different. It is thought that differences in humidity cause the electrical conductivity to become non-uniform, which disturbs the shape of the electric field inside the gel, but this has not been clearly elucidated.

On the other hand, the electric field lines between both rod-shaped or plate-shaped electrodes are not perfectly straight lines, and as shown in Figure 2, as the two electrodes fall from the center to the sides, the 1u field lines become convex in the direction of the sides between the electrodes. It will take on a shape. This phenomenon is caused by the fact that both rod-shaped or flat-shaped electrodes have a finite length and each has both ends, so the four-potential line between the two poles is not a straight line parallel to the electrodes, as shown at point H in Figure 2, but a line between the two magnetic poles. This is because the center of the two electrodes deviates from the straight line a as it goes down from the center of each electrode.

The fact that the electric field lines in the gel have the shape shown in Figure 2 somewhat prevents the electrophoretic image from having the shape shown in Figure 1 (especially in the lower half of the gel). However, even if it is clear that the electric field is not linear and the electrophoretic image has the shape shown in Figure 1, it still means that a linear electric f-wall cannot be obtained. Meaning, not good.

The purpose of the present invention is to overcome these drawbacks of conventional electrophoresis apparatuses and to provide an apparatus in which electrophoresis of a living body is carried out in a straight line and in parallel no matter where the body is located. be.

The structure of the present invention is achieved by forming both electrodes into closed ring shapes with no ends, and forming frames on both sides of the electrophoretic membrane into cylindrical shapes having the same cross-section as the outer or inner edges of the electrodes. Ru.

1 in Figure 3. 2. 3 is an embodiment showing an electrode and frames on both sides of the present invention. 1 in FIG. 6 shows an embodiment in which both electrodes 11 and 12 are each shaped like a circular ring, and both frames 51 and 32 are each shaped like a cylinder, and 2 in FIG. An embodiment is shown in which both frames 31 and 32 are each shaped like an elliptical cylinder, and 3 in FIG. 3 indicates both electrodes 11 and 12.
An embodiment is shown in which each of the frames 31 and 32 is formed into a polygonal ring shape, and each of the frames 31 and 32 is formed into a polygonal cylinder shape.

In the present invention having the above configuration, each electrode and the frames 31 and 32 on both sides of the electrophoretic membrane do not have outer ends, and therefore the electrophoretic membrane also does not have outer ends, so that the electrophoretic state of the biological fluid is As shown in FIG. 4, it has a substantially linear shape, which is extremely convenient for analyzing electrophoretic images. The reason for this result is that because the electrophoretic membrane does not have an outer edge, there is no deviation in temperature and humidity between the areas near both ends and the other areas, unlike in the case of conventional devices. This is thought to be due to the fact that drift of the biological fluid and deviation in electrical conductivity do not occur, and that the electric field lines within the electrophoretic region are linear as shown in 1.2.5 of FIG. (A detailed analysis of the same is currently under consideration.)

In the present invention, both frames 51 and 32 are cylindrical, so when designing the spacer to migrate in the vertical direction, there is no need for a support for stably standing the spacer upright, which is convenient.

[Brief explanation of drawings]

FIG. 1 shows an electrophoretic image of a biological fluid obtained by a conventional 'Kameki HliJI apparatus. Fig. 2 shows equipotential lines and electric field lines between two parallel rod-shaped or flat plate-shaped electrodes of equal finite size. 1 in Fig. 3 shows an embodiment in which both electric poles are circular and the picture frame is cylindrical (the lower part shows an elevational view of the electrodes and the frame). 2 in Figure 3 shows an example in which both electrodes are shaped like elliptical rings and both frames are shaped like elliptical cylinders (the lower part shows an elevational view of the electrode and the frame). 6 in Fig. 3 shows an embodiment in which one of the two cars has a polygonal ring and both frames have polygonal rings (the lower part shows an elevational view of the electric fault 2 and the frame). 11.12: Both electrodes, 2: Direct electrophoresis #I membrane, 4: Receiver for storing biological fluid, 31.32: Frame Figure 4 shows electric field lines in the electrophoretic membrane in the apparatus of the present invention. W 4 Fan, Figure 5, l Fan, S Peng, 2 Fan, Figure 5, 3 Procedural amendment August 2, 1980 Director of the Japan Patent Office Kazuo Wakasugi 1, Indication of the case 1981 Patent Application No. 081552 2. Title of the invention Ring type electrophoresis device 3. Relationship with the amended case Patent applicant Furigana Setagaya Sakuragaoka Address Kitozaki, 5-38-1 Sakuragaoka, Setagaya-ku, Tokyo
Takeshi Representative Director Takeshi Kidozaki 4. Add the following to the line below line 12 on page 6 of the brief explanation of the drawings in the agent's statement 7 and the statement of contents of the amendment. Record

Claims (1)

[Scope of Claims] (1) Both electrodes are each in the shape of a closed ring without an end, and the frames on both sides of the electrophoretic membrane are each in the shape of a cylinder having a cross section that is the same as the outside or edge of both electrodes. A ring-type electrophoresis device. (2) The ring-type electrophoresis device according to claim (1), wherein both electrodes are each circular and the frames on both sides are each cylindrical. (6) A ring-type electrophoresis device according to the claims, wherein both electrodes each have an elliptical ring shape, and the frames on both sides each have an elliptical cylindrical shape. (4) The ring-type electrophoresis device according to claim (1), wherein both electrodes each have a polygonal ring shape, and the frames on both sides each have a polygonal cylinder shape.