JPS59187263A - Blood separation tube - Google Patents

Abstract

PURPOSE:To obtain a blood separation tube for sampling plasma which enables accurate measurement of the coagulation time in the diagnosis of blood agglutination troubles by arranging a thixotropic separating agent with the specific gravity intermediate between plasma and a blood cell component and an anti-blood agglutination agent provided into blood dispersably in contact therewith in a bottomed blood sampling tube. CONSTITUTION:In the inspection of an agglutination of blood, a thixotropic separating agent 15 with the specific gravity intermediate between plasma and a blood cell component is placed on the bottom of the body 11 of a blood sampling tube 10 while an anti-blood agglutination agent 12 is put on a separating agent 15 and a puncturable plug 14 made of butyl rubber or the like is applied to enable the holding the inside of the body 11 at a negative pressure. The blood sampling tube 10 uses a blood sampling needle 17 on a blood sampling holder 16 to sample blood from a patient. After the sampling of the blood, the tube is tilted to mix and a centrifugal separation is performed to separated a plasma layer 18 on the separating agent 15 and a blood cell layer 19 thereunder. After the separation, components of the layers 18 and 19 is kept from being mixed during the preservation and transportation with dispersion of the anti- agglutination agent 12 thereby facilitating the withdrawal of plasma only. Thus, plasma component can be used for inspection of agglutination to obtain a sample allowing an easy and accurate diagnosis of blood agglutination troubles.

Description

DETAILED DESCRIPTION OF THE INVENTION 1. Background Technical Field of the Invention The present invention relates to a blood separation tube used for collecting a sample as a clinical test to enable testing for abnormalities in coagulation factors involved in the hemostasis mechanism of blood.

BACKGROUND OF THE INVENTION Known blood coagulation system testing methods include a plasma calcium time measurement method, a plasma prothrombin level measurement method, a partial thromboplastin time measurement method, and a thromboplastin formation test.

In each of the above-mentioned conventional blood coagulation system testing methods, there is a bottomed tube body that allows blood to be collected, and 3, which is loaded into the tube body.
Blood containing anticoagulants such as Section 8 sodium citrate solution, Section 3.13 sodium citrate solution, and 0.1M sodium oxalate solution, and a plug fitted into the mouth of the tube body. Blood collection tubes for coagulation system testing are used.

That is, in each of the above conventional blood coagulation system testing methods,
After collecting blood into the blood collection tube, it is necessary to centrifuge the blood collection tube to separate plasma components and blood cell components, transfer the separated plasma components to another container with a pipette, etc., and then By adding other reagents determined according to each test method to the plasma components and measuring the coagulation time of the plasma, it is possible to diagnose various coagulation disorders in the blood to be tested.

However, when using the above-mentioned conventional blood collection tube, it is necessary to transfer the centrifuged plasma component to another container, and during the transfer operation, a pipette or other instrument is inserted into the plasma component. When doing so, there is a risk that plasma components may be contaminated by floating blood cell components. In this way, when 11y of plasma is contaminated by blood cell components (
'', the transparency of the plasma decreases, and the completion of coagulation occurs in the plasma in an instant, making it impossible to quickly determine the final stage of coagulation. Correct measurement of clotting time is the key to determining coagulation disorders in the blood being tested. It is difficult to make an accurate diagnosis.

Furthermore, if the effect of the anticoagulant is insufficient, blood cell components may become entangled with the fiber cords in the plasma, making test results inaccurate.

■ Purpose of the Invention The present invention allows a predetermined coagulation system test reagent to be mixed with collected blood, without transferring it to another container.
To provide a sampling tube for blood coagulation system testing, which uses plasma components that are not contaminated by blood cell components as a sample and enables easy and accurate diagnosis of coagulation disorders in flannel blood. With the goal.

In order to achieve the above object, the blood separation tube according to the present invention (
4. A bottomed blood collection tube body with -M of the tube body open and the other end closed, a punctureable stopper that seals the opening of the cough tube body, and a tube body housed in the tube body and separated. A thixotropic separating agent having an intermediate specific gravity between the blood plasma component and the upper blood cell component; After the blood coated with a coagulant and prevented from coagulating is centrifuged, blood cell components are isolated from the blood.

Further, in the present invention, the inside of the blood separation tube is maintained at a reduced pressure state corresponding to a predetermined amount of blood to be collected.

(2) Detailed Description of the Invention FIG. 1 is a sectional view showing a blood collection tube 10 according to an embodiment of the present invention. The blood collection tube 10 includes a bottomed tube body 11 that allows blood to be collected, and an anticoagulant 1 loaded inside the V body 11.
2, the frame body 1 fitted into the mouth portion 13 of the tube body 11;
4. Further, the tube body 11 of the blood collection tube 10 is loaded with a gel-like drug 15 for separating plasma components and blood cell components collected and separated by centrifugation.

Note that the separating agent 15 (1) is initially located at the bottom side of the tube body 11, and the anticoagulant 12 ('', initially located at the top of the separating agent 15).

The tube body 11 (made of transparent glass, plastic, etc.), and the plug body 14 made of butyl rubber, etc.
The inside of the tube body 11 is sealed, and a predetermined depressurized state corresponding to a predetermined blood collection song can be formed and maintained inside the tube body 11, and as shown in FIG. It is possible to pierce the blood collection needle 17 through the blood collection needle 17.

As the anticoagulant 12, drugs specified according to each blood coagulation test method to be tried, such as 3.8% sodium citrate solution, 3.13% sodium citrate solution, 0.1M oxalic acid. Sodium solution is used. Note that one amount of the sodium citrate solution is used for every nine volumes of blood.

As mentioned above, the separating agent 15 is initially located at the bottom side of the tube body 11 and is substantially hydrophobic and thixotropic, and is substantially inert to blood. , and has a specific gravity intermediate between the plasma component and blood cell component to be separated. Therefore, when the separating agent 15 is subjected to centrifugal separation against the blood collection tube 10 from which blood has been collected, it exhibits fluidity and moves to the boundary between the two components, and as the centrifugal force stops, the two components are separated from each other. Forms a semi-solid or non-fluid wall.

Here, as the separating agent 15, known ones can be used, for example, those having a viscosity of 10,000 to 80,000 cp
(25℃) containing α-olefin/maleic acid diester copolymer as the main component or silicone as the main component,
Add a viscosity/specific gravity regulator to this, specific gravity 1.03
5 to 1.055, etc. are used.

■Specific operation of the invention First, the outer end 17A of the blood collection needle 17 is punctured into the patient's vein, and the inner end 17B of the blood collection needle is inserted into the stopper 14 of the blood collection tube 1o inserted into the blood collection boulder 16. Due to its strength,
Blood is collected into the tube body 11 which is in a depressurized state.

After the blood collection, the blood and anticoagulant 12 are mixed by inversion,
After preventing blood coagulation, the blood collection tube 1o is placed in a centrifuge. Through this centrifugation, blood is separated into plasma component 18 and blood cell component 19 in which coagulation has been prevented, and both components 18
．． 19 are fractionated by the separating agent 154ζ and separated from each other, as shown in FIG.

The plasma component 18 separated as described above is separated by the separating agent 1.
Since it is isolated from the blood cell component 19 by the blood cell component 19, it can be used as a correct sample for coagulation system testing without being contaminated by the blood cell component 19. That is, the plasma component 18 separated by the separating agent 15 is
From this, other reagents specified according to each blood coagulation system test method are added as is without being transferred to another container, and the clotting time of the plasma component 18 is measured.
This enables the diagnosis of various coagulation disorders in sample blood.

In addition, the blood collection tube 1o can be used as a sample storage container as it is after being separated from the plasma component 18 (!:, blood cell component 19), and even when the blood collection tube 1o is stored and transported, the plasma component 18 and the blood cell component 19 can be used as is. This eliminates the possibility of mixing with the blood cell component 19.In addition, the plasma component 1 as a sample stored in the blood collection tube 1o is eliminated.
8 can be removed at any time very easily and efficiently by decantation, pipetting, etc.

In addition, an experiment was carried out in which blood collection tubes 10, which were stored in width such that plasma component 18 and blood cell component 19 were separated by separating agent 15, were rapidly frozen in a -20°C deep freezer. As a result, it was found that the glass tube body 11 was not damaged and that the separating agent 15 was stable.

■Specific Effects of the Invention As described above, the blood separation tube according to the present invention has a blood collection tube body with a part of the tube open and the other end closed, and the opening of the tube body sealed. a thixotropic separation agent housed in the tube body and having a specific gravity intermediate between the plasma component and blood cell component to be separated; and a space inside the tube body that is in contact with the plug body. An anticoagulant dispersible in the blood by contact with the blood is added to the blood, and after the blood whose coagulation is prevented is centrifuged, blood cell components are isolated from the blood. This is what I did. Therefore, it is possible to mix collected blood with a coagulation system test reagent and provide plasma components that are not contaminated by blood cell components as samples for coagulation system tests without transferring to another container. It becomes possible to easily and accurately diagnose coagulation disorders in sampled blood. Also, move or
Blood clots and blood cells will not be mixed into plasma components even when falling. Because the plasma components are transferred in a leather case, there is no exposure to outside air, and if air contamination is prevented, the time required from blood collection to testing is greatly shortened. Furthermore, after centrifugation, it can be used as it is in a sample storage container, and blood sugar levels can also be measured without transferring it to another container. In addition, since the plasma volume after centrifugation is completely separated from blood cell components by a layer of separating agent, there is no risk of blood cell components becoming entangled with fibrin in the plasma. Even if solidification is not sufficiently performed, the results of the food inspection can be more accurate than in the case where a conventional separation agent cannot be used.

Further, in the present invention, the inside of the blood separation tube is maintained at a reduced pressure of 1 level corresponding to a predetermined amount of blood to be collected.

[Brief explanation of drawings]

Fig. 1 is a sectional view showing a blood collection tube according to a first embodiment of the present invention, Fig. 2 is a sectional view showing the state of loading the blood collection tube into a blood collection boulder, and Fig. 3 is a sectional view showing blood collection using the same blood collection tube. It is a sectional view showing soft stool. 10-... blood collection tube, 11 tube main body, 12...
anticoagulant, 13. oral part, 14... plug body,
15 Separation agent, 18. Plasma component, 19 Blood cell component. Patent Applicant Chil-Nana Co., Ltd. Agent Patent Attorney Osamu Shiokawa

Claims (2)

[Claims] (1) A bottomed blood collection tube body with a part of the tube open and the other end closed, a punctureable stopper that seals the opening of the nuclear tube body, and a stopper housed within the tube body and separated. a thixotropic separating agent having an intermediate specific gravity between plasma components and blood cell components; and an anticoagulant dispersible in the blood by contact with the blood, provided in the space inside the tube body and in contact with the plug body. After the blood containing the anticoagulant and anticoagulation is centrifuged,
A blood separation tube characterized in that blood cell components are isolated from the blood. (2) The blood separation tube according to claim 1, wherein the inside of the blood separation tube is maintained at a reduced pressure corresponding to a predetermined amount of blood to be collected.