JPS59170013A - Substance for preventing infection of fishes - Google Patents

Abstract

PURPOSE:To secrete an antimicrobial substance on viscous liquid of the surface of fish bodies, to prevent stress, and infectious diseases of fishes, by using pantethine as an active ingredient, feeding it to fishes. CONSTITUTION:A substance for preventing infection of fishes, containing pantethine as an active ingredient. Objects of infectious diseases are aeromonas disease and ulcer disease of carp, red pest and brachitis of eel, furunculosis of Oncorhynchus masou, rainbow trout, etc., vibriosis of sweet fish, yellow tail, red sea bream, rainbow trout, etc. 1-100mg/fish weight kg/day as pantethine is medicated. Pantethine usually suspended or dissolved in fats and oils (e.g., feed oil, etc.) for fish farming or in water is added to a feed and adsorbed on it just before its use and fed to fishes. It is considered that by feeding pantethine a substance having antimicrobial activity is secreted on viscous liquid of fish bodies, and it prevents infection by pathogenic germs.

Description

[Detailed Description of the Invention] Infectious diseases of fish are caused by stress caused by the deterioration of the environment in which the fish live, such as changes in water temperature and lack of oxygen.

They occur frequently when stress such as overcrowding and increases in ammonia and nitrite nitrogen occur, increasing the damage caused. To improve the environmental deterioration that causes stress to these fishes,
In farmed fish, etc., there is an inverse relationship with productivity improvement.
It is not necessarily something that can be resolved immediately.

As a result of intensive research into infectious diseases of fish from the viewpoints of environment and stress, the inventors of the present invention discovered that administration of pantethine is effective in preventing stress and preventing infectious diseases of fish, and completed the present invention.

Next, the effects will be explained using examples.

Example Magoi 8 with an average weight of 2859 (average body length 20.4 cm)
The nine fish were divided into three groups of 18 fish each, and each group was housed in a 2-ton aquarium. Group 1 was normally reared at a water temperature of 25°C, and Groups ■ and ■ were reared under stress every day in which the water temperature was raised by 6°C over 12 hours a day and then lowered by 6°C over the next 12 hours.

As feed, commercially available adult carp pellets were given at a rate of 1% of body weight per day. Group (2) was treated as a pantethine administration group, and pantethine was added to the feed to give an amount of 100 m9 per body weight.

After three weeks of breeding, the test fish were taken and their blood properties were determined.

The antibacterial activity of body surface mucus and the number of bacteria attached to each part of the fish body were measured. The results are shown in Tables 1 to 8, and the blood properties decreased in Group 2, which was subjected to water temperature stress, but did not decrease in Group 2, which received pantethine while applying the same stress.

The pantethine administration group (■ group) was also superior in terms of the antibacterial activity of body surface mucus and the number of attached bacteria, and the infection prevention effect was observed.

Table 1: Blood properties (n-5, mean ± SD) Table 2: Antibacterial activity of body surface mucus (distance between inhibition diameters, η -5) After washing the fish body with sterile distilled water, scrape off the mucus with a spatula. , smear this onto a paper disk with a diameter of 8 chicks. This is placed on an ordinary agar medium coated with a test strain (AeromOnas), cultured at 25°C for 24 hours, and the diameter of the inhibition is measured.

Table 3: Number of attached bacteria (LOg/ri, 5. mean±SD) The sample is homogenized with sterile physiological saline, and the number of bacteria is determined by a conventional method.

When pantethine is administered to fish, a substance with antibacterial activity is secreted into the mucus on the body surface, and this is thought to prevent infection by pathogenic bacteria.

The target fish and infectious diseases include carp aeromonas disease, pitting disease, eel fin red disease, parakoro disease, gill disease,
Se of wild trout, rainbow trout, etc.

Examples include sow disease, and vibrio diseases of sweetfish, yellowtail, red sea bream, rainbow trout, etc.

The dosage is 1 to 1 g of pantethine per gram of fish weight.
A range of 1,001,1197 days is appropriate; usually, pantethine suspended or dissolved in oil for fish farming (feed oil, etc.) or water may be mixed and adsorbed with feed immediately before use, and fed to fish.

It is generally added to feed at a rate of 0.0601 to 11%.

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Claims (1)

[Claims] Fish infection prevention product containing pantethine as an active ingredient