JPS591500A - ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法 - Google Patents

ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法

Info

Publication number
JPS591500A
JPS591500A JP57110999A JP11099982A JPS591500A JP S591500 A JPS591500 A JP S591500A JP 57110999 A JP57110999 A JP 57110999A JP 11099982 A JP11099982 A JP 11099982A JP S591500 A JPS591500 A JP S591500A
Authority
JP
Japan
Prior art keywords
gene
plasmid
dna
promoter
plasmid vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP57110999A
Other languages
English (en)
Japanese (ja)
Other versions
JPS6237958B2 (enExample
Inventor
Masahiro Iwakura
正寛 巌倉
Yukio Shimura
志村　幸雄
Keishiro Tsuda
津田　圭四郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP57110999A priority Critical patent/JPS591500A/ja
Publication of JPS591500A publication Critical patent/JPS591500A/ja
Publication of JPS6237958B2 publication Critical patent/JPS6237958B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP57110999A 1982-06-28 1982-06-28 ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法 Granted JPS591500A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP57110999A JPS591500A (ja) 1982-06-28 1982-06-28 ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP57110999A JPS591500A (ja) 1982-06-28 1982-06-28 ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法

Publications (2)

Publication Number Publication Date
JPS591500A true JPS591500A (ja) 1984-01-06
JPS6237958B2 JPS6237958B2 (enExample) 1987-08-14

Family

ID=14549830

Family Applications (1)

Application Number Title Priority Date Filing Date
JP57110999A Granted JPS591500A (ja) 1982-06-28 1982-06-28 ジヒドロ葉酸還元酵素遺伝子を利用したプロモ−タ検出用プラスミドベクタ−及びその製造方法

Country Status (1)

Country Link
JP (1) JPS591500A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102660394B1 (ko) * 2019-03-11 2024-04-24 에이치엘만도 주식회사 전동 파워스티어링 시스템의 오버레이 제어 장치 및 그 방법

Also Published As

Publication number Publication date
JPS6237958B2 (enExample) 1987-08-14

Similar Documents

Publication Publication Date Title
Mizuuchi et al. Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulation
AU2014335915B2 (en) Modified helicases
EP0035384B1 (en) Deoxynucleotide linkers to be attached to a cloned dna coding sequence
JP2021534798A (ja) ゲノムを調節するための方法及び組成物
US9885026B2 (en) Modified cascade ribonucleoproteins and uses thereof
KR20190082318A (ko) Crispr/cpf1 시스템 및 방법
CN110408636B (zh) 多重标签串联的dna序列及其在蛋白质表达纯化系统的应用
CN110408635B (zh) 一种含有链霉亲和素元件的核酸构建物在蛋白质表达、纯化中的应用
US5318900A (en) Method for producing antiviral protein utilizing E. coli transformant, and gene and E. coli vector used in the method
JP2005514025A (ja) タンパク質発現及び精製のための方法及び組成
Peñaloza-Vázquez et al. Characterization of CorR, a transcriptional activator which is required for biosynthesis of the phytotoxin coronatine
AU2021350637A1 (en) Systems and methods for transposing cargo nucleotide sequences
Yang et al. Action at a distance for negative control of transcription of the glpD gene encoding sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12
US20240301371A1 (en) Crispr-associated transposon systems and methods of using same
KR101373297B1 (ko) 대장균 포스포글리세르산 인산화효소 유전자를 융합 파트너로서 포함하는 발현벡터
CA3209991A1 (en) Crispr-associated transposon systems and methods of using same
CN116438302A (zh) 用于对货物核苷酸序列转位的系统和方法
US5389526A (en) Plasmid vectors for cellular slime moulds of the genus dictyostelium
EP0622460A2 (en) Plasmid and escherichia coli transformed with it
Pérez-Martin et al. Design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease.
KR100803095B1 (ko) 바실러스 서브틸리스 ｃｈ２ 유래의 키토산아제를 코딩하는유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로형질전환된 형질전환체 및 이로부터 생산되는 단백질의정제방법
DK172695B1 (da) DNA-afsnit af trp-operonet fra E. coli, plasmider indeholdende DNA-afsnittet, fremgangsmåde til fremstilling af en vektor i
Küchler et al. From nuclear genes to chloroplast localized proteins
JPS6237958B2 (enExample)
KR20250144914A (ko) 목적 단백질의 세포외 분비용 발현벡터 및 이의 용도