JPS5910620Y2 - Cartridge for multilayer chemical analysis materials - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cartridge for analyzing liquid samples using an integrated multilayer chemical analysis material consisting of a porous spreading layer, a reagent layer, and a support.

This cartridge is characterized by having a hollowed-out window in the lower storage wall, and the upper wall serving as a moisture evaporation prevention cover that can be opened and closed.

Analysis sheets for aqueous solution samples, especially multilayer integrated chemical analysis sheets for simple and rapid quantitative analysis of certain components in biological fluids such as blood and urine, are disclosed in Japanese Patent Application Laid-Open No. 49-53888 and U.S. Patent No. 3,992,158. It is publicly known.

Such an analysis sheet has a transparent support 1 as shown in FIG.
It has a structure in which one or more reagent layers 2 are stacked on top of the reagent layer 2, and a porous development layer 3 is stacked on top of the reagent layer 2 so as not to separate from each other.

The liquid sample dropped onto this analysis sheet spreads uniformly through the developing layer and at the same time penetrates into the reagent layer and reacts, resulting in coloring or discoloration, and the extent of the reaction can be observed and quantified through the support. can.

In this quantitative determination, a certain fraction in a liquid sample meets a reagent in a reagent layer and reacts there, but this reaction often uses a color reaction with an organic reagent in water; A fast reaction takes 1 minute or more to complete, while a slow reaction, such as one using an enzyme as a reagent, often takes 5 to 10 minutes or more under heating at 35°C.

Furthermore, it is necessary that sufficient moisture be present at the site until the reaction is completed.

Actually, 5 to 20 μl of the sample was dropped onto the analysis sheet,
A comparison was made between one that was left open and one that was covered with a thin film immediately after the spot liquid had soaked into the developing layer after dropping.

In the former case, the spot area began to dry rapidly and the coloring reaction stopped progressing, but in the latter case, the spot area began to dry rapidly and the coloring reaction stopped progressing.
It was found that the coloring reaction progressed satisfactorily as the sample was kept in a wet state for 0 minutes or more.

Therefore, in order to use a new type of multilayer integrated chemical analysis sheet for simple and rapid quantitative determination, after spotting a liquid sample on the analysis sheet, immediately cover it.
It was found that it is an essential operation to prevent moisture from evaporating from the spot 1 and drying out.

In addition, after the coloring reaction is completed in the reagent layer after dropping the sample, the color density is measured from the transparent support side. It is necessary to ensure that there is no problem with measurement.

The present invention consists of a lower storage wall with a hollowed-out window, a multilayer chemical analysis material placed on the hollowed-out window and consisting of a porous development layer, a reagent layer, and a support, and a structure that can be opened and closed above them. This cartridge is constructed from a moisture evaporation prevention cover.

One end or one side of the moisture evaporation prevention cover may be fixed to one end or one side of the storage wall so that it can be opened and closed.

Further, a side wall having a groove may be provided on at least two opposing sides of the storage wall portion, and the moisture evaporation prevention cover may be arranged to slide in and out along the groove to open and close.

Regarding the materials constituting the porous spreading layer and reagent layer of the multilayer chemical analysis material used in the present invention, their manufacturing method and structure, in addition to the above-mentioned patent specifications, JP-A-50-137192 and JP-A-51-40191 can be found. , No. 52-3488, No. 52
The materials, manufacturing methods and structures described in No. 131786 and No. 52-142584 can be used as they are.

As for the support, the supports described in each of the above-mentioned patent specifications can be used.

The method of combining the spreading layer, reagent layer, and support can also be carried out according to the methods described in the aforementioned patent specifications.

The multilayer chemical analysis material used in the cartridge of the present invention is preferably in the form of a sheet or a thin tape.

The cartridge of the present invention will be explained with reference to embodiment drawings.

The cartridge shown in FIG. 2 has a lower storage wall portion 4 (hereinafter referred to as a lower leaf only for the cartridge shown in FIG. 2).

) is provided with a cut-out window (opening) 5 in the middle, and a moisture evaporation prevention cover 6 is fixed to one end or one side of the lower leaf 4 so that it can be opened and closed.

The analysis sheet is pasted in advance at position 7 on the lower leaf 4, the sample drop is spotted at the position corresponding to the part visible from below through the window 5 on the analysis sheet, and then the cover 6 is placed on the analysis sheet. Cover.

By doing so, it is possible to efficiently store the analysis sheet, prevent moisture from evaporating from the spot portion, and perform measurements from below the window 5 after the reaction is completed.

The materials used for the lower leaf and the cover of the present invention may be different, for example, the bar may be a flexible film and the lower leaf may be a hard plastic sheet.

Or fold the same piece of film and overlap it, ■
One side may be the lower leaf and the other side may be the cover.

Materials for the cover include aluminum foil, paraffin paper, laminated paper, waterproof paper, and composite or resin films such as polyethylene, polypropylene, polyvinyl chloride, saran, polyester, and cellulose ester.

In addition to the materials that can be used in common with the above-mentioned cover, the lower leaf can be made of a wide variety of materials such as metal, wood, cardboard, polystyrene, polymethacrylate, and polyurethane.

FIG. 3 shows another embodiment of a cartridge in which the moisture evaporation prevention force bar is opened and closed in a sliding door manner.

Lower storage wall portion 8 having grooves on at least two opposing sides
A hollowed-out window (opening) 5 is provided in the middle, and a moisture evaporation prevention cover 9 that opens and closes like a sliding door is inserted.

Place the analysis sheet at position 7, and after dropping the sample, insert the cover 9 all the way to cover it.

Note that the stopper (the side wall on which the moisture evaporation prevention cover hits and stops) 10 may be omitted; if there is no stopper, the moisture evaporation prevention cover can be pulled out to either the left or right of the storage wall section as shown in Figure 3. It is convenient because you can open and close it with just one hand.

The materials that make up the storage wall and moisture evaporation prevention cover are as follows:
It is possible to use each of the materials in the embodiment of FIG. 2 described above.

However, as a matter of course, it is preferable that the sliding door-type moisture evaporation prevention cover be made of a film that is thicker or more rigid than that of the moisture evaporation prevention cover in the embodiment shown in FIG.

[Brief explanation of the drawing]

Figure 1 is a schematic diagram showing a cross section of an integrated multilayer chemical analysis material.
FIGS. 2 and 3 show an embodiment of the liquid sample chemical analysis cartridge of the present invention. The pass indicates the direction in which the moisture evaporation prevention cover is opened and closed.

Claims (3)

[Scope of utility model registration request] (1)! It consists of a storage wall with a cut-out window, an integrated multi-layer chemical analysis material placed on the cut-out window, and a moisture evaporation prevention cover that is openable and closable above them. The analytical material comprises a transparent support, a reagent layer, and a porous spreading layer capable of spreading an aqueous liquid sample so as to supply a constant volume of the aqueous liquid sample per unit area to the reagent layer. Liquid sample chemical analysis cartridge. (2) The cartridge according to claim (1), wherein one end or one side of the moisture evaporation prevention cover is fixed to one end or one side of the storage wall so as to be opened and closed. (3) The storage wall has a side wall having grooves on at least two opposing sides, and the moisture evaporation prevention cover is arranged so that it can be opened and closed by sliding in and out along the groove. A cartridge according to claim (1) for patent registration.