JPS5898084A - Reactor containing immobilized enzyme - Google Patents

Reactor containing immobilized enzyme

Info

Publication number
JPS5898084A
JPS5898084A JP19501981A JP19501981A JPS5898084A JP S5898084 A JPS5898084 A JP S5898084A JP 19501981 A JP19501981 A JP 19501981A JP 19501981 A JP19501981 A JP 19501981A JP S5898084 A JPS5898084 A JP S5898084A
Authority
JP
Japan
Prior art keywords
enzyme
reactor
immobilized
substrate solution
fibers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP19501981A
Other languages
Japanese (ja)
Other versions
JPS6025112B2 (en
Inventor
Yasuo Kihara
木原 康夫
Takashi Kawasaki
隆志 川崎
Keiichi Ushiyama
敬一 牛山
Shuji Senda
千田 修治
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Corp
Original Assignee
Nitto Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Electric Industrial Co Ltd filed Critical Nitto Electric Industrial Co Ltd
Priority to JP19501981A priority Critical patent/JPS6025112B2/en
Publication of JPS5898084A publication Critical patent/JPS5898084A/en
Publication of JPS6025112B2 publication Critical patent/JPS6025112B2/en
Expired legal-status Critical Current

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To obtain a reactor having a reactor activity without causing clogging, by means of assemblies, consisting of fibers containing immobilized enzymes, and spaced apart in the flow direction of a substrate solution in a vessel. CONSTITUTION:A vessel 1 is divided into plural compartments 3 by porous separators 2 having holes for passing a substrate solution therethrough in the flow direction of the substrate solution. Assemblies 5 of fibers containing immobilized enzymes are packed in the respective compartments 3 to leave spaces 4 in the upper parts of the compartments 3. The vessel 1 has an inlet 6 of the substrate solution at the top and an outlet 7 of a reaction product at the bottom.

Description

【発明の詳細な説明】 本発明は固定化酵素反応器に関する。[Detailed description of the invention] The present invention relates to immobilized enzyme reactors.

固定化酵素を用いる反応は、従来、ビーズ状担体に酵素
を固定化し、これを容器内に充填したり、また、膜状担
体に酵素を固定化し、適宜のスペーサと共にら旋状の構
造体や積層した構造体とし、これを容器に充填し、こう
して形成した反応器に基質溶液を供給することにより行
なわれている。
Conventionally, reactions using immobilized enzymes involve immobilizing the enzyme on a bead-like carrier and filling it into a container, or immobilizing the enzyme on a membrane-like carrier and forming a helical structure or a spiral structure with an appropriate spacer. This is carried out by forming a laminated structure, filling it into a container, and supplying the substrate solution to the reactor thus formed.

しかし、このような反応器によれば、固定化酵素の表面
積が小さいので、一般に反応器の容積当りの酵素活性が
十分に大きくないうえに、固定化酵素かビーズ状の場合
、容易に圧密化して目詰まりを起こし、反応器活性が速
やかに低下し、また、固定化酵素が膜状の場合、その交
換が必らずしも容易ではない。更に、従来の酵素反応器
においては、固定化酵素の充填層や構造体に不均一性や
欠陥がある場合、反応器内全体にわたって基質溶液流に
優先流或いはチャネリングが生じ、このような点からも
反応器活性が低下することが多い。
However, in such a reactor, since the surface area of the immobilized enzyme is small, the enzyme activity per reactor volume is generally not large enough, and if the immobilized enzyme is in the form of beads, it is easily compacted. If the immobilized enzyme is in the form of a membrane, it is not always easy to replace it. Furthermore, in conventional enzyme reactors, if there is non-uniformity or defect in the packed bed or structure of the immobilized enzyme, preferential flow or channeling of the substrate solution flow occurs throughout the reactor. reactor activity often decreases.

本発明は上記した問題を解決するためになされたもので
あって、反応器活性が高(、且つ、固定化酵素が圧密化
や目詰まりを起こさず、また、反応器において基質溶液
が効果的に分配され、従って、高い反応器活性が長期に
維持される酵素反応器を提供することを目的とし、更に
別の目的は固定化酵素を容易に交換することかできる固
定化酵素反応器を提供することである。
The present invention was made to solve the above-mentioned problems, and it has a high reactor activity (and the immobilized enzyme does not cause compaction or clogging), and the substrate solution is effective in the reactor. Another object of the present invention is to provide an enzyme reactor in which the immobilized enzyme can be easily exchanged. It is to be.

本発明による酵素反応器は、繊維状担体に酵素か固定化
された酵素固定化繊維からなる集合体が基質溶液の流れ
方向に間隔をおいて分離して容器内に配設されているこ
とを特徴とする。
In the enzyme reactor according to the present invention, aggregates of enzyme-immobilized fibers in which enzymes are immobilized on a fibrous carrier are arranged in a container separated at intervals in the flow direction of the substrate solution. Features.

本発明において用いる酵素固定化繊維は、繊維状担体に
酵素が固定化されているものであって、担体としての繊
維は、固定すべき酵素の種類に応じて適宜に選ばれるか
、例えばポリアミド、ポリビニルアルコール、セルロー
ス、68セルロース、コラーゲン等の合成又は天然重合
体からなる繊維か用いられる。繊維担体への酵素の固定
化方法は特に制限されず、従来より知られている物理吸
着法、イオン結合法、包括法、共有結合法等が適宜に用
いられる。
The enzyme-immobilized fiber used in the present invention has an enzyme immobilized on a fibrous carrier, and the fiber as the carrier may be appropriately selected depending on the type of enzyme to be immobilized, or may be made of, for example, polyamide, Fibers made of synthetic or natural polymers such as polyvinyl alcohol, cellulose, cellulose 68, collagen, etc. are used. The method for immobilizing the enzyme on the fiber carrier is not particularly limited, and conventionally known physical adsorption methods, ionic bonding methods, entrapment methods, covalent bonding methods, etc. can be used as appropriate.

本発明に部いて、かかる酵素固定化繊維の集合体とは、
繊維か集束され、又は集合されて形成する塊状物又はシ
ート状物をいい、具体例としては芯材に巻付けた繊維の
集合体、網布等で包んだ繊維の集合体、不織布様の集合
体、又はこれを積層固定した繊維の集合体等を挙げるこ
とができ、更には反応器内に充填されたときに形成する
繊維の集合体をも含む。尚、このような#素固定化縁維
の集合体については、繊維状担体に酵素を固定化し、こ
れを集合体に形成してもよ(、或いは繊維状担体を集合
体に形成した後、これに酵素を固定化してもよい。
In the present invention, such an aggregate of enzyme-immobilized fibers is defined as
A lump or sheet-like object formed by bundling or aggregating fibers; specific examples include an aggregate of fibers wrapped around a core material, an aggregate of fibers wrapped in net cloth, etc., and an aggregate like non-woven fabric. Examples include fiber aggregates formed by laminating and fixing these bodies, and further include fiber aggregates formed when filled in a reactor. In addition, for such an aggregate of #element-immobilized edge fibers, the enzyme may be immobilized on a fibrous carrier and this may be formed into an aggregate (or, after forming the fibrous carrier into an aggregate, Enzymes may be immobilized thereon.

本発明においては酵素固定化繊維の集合体は、少なくと
も二つが間隔をおいて相互に分離して容器内に配設され
て、反応器を形成する。第1図は竪型反応器の一実施例
を示す。容器lは、基質溶液を通過させるための孔を有
する多孔体のセパレータ2により基質溶液の流れ方向に
複数室に分画され、各分画室3には上部に空間4を残す
ように酵素固定化繊維の集合体5が充填されており、上
端に基質溶液人口6、下端に反応生゛成物出ロアを有す
る。かくして、上記空間により一つの分画室から次の分
画室への基質溶液の流れを攪拌し、優先流の生起を効果
的に防止する。必要ならば、上記空間による基質溶液の
流れの攪拌効果を高めるために、空間に一枚又は二枚以
上の邪魔板8が配設される(一部のみ図示)。
In the present invention, at least two aggregates of enzyme-immobilized fibers are separated from each other at intervals and arranged in a container to form a reactor. FIG. 1 shows an embodiment of a vertical reactor. The container l is divided into a plurality of chambers in the flow direction of the substrate solution by a porous separator 2 having holes for passing the substrate solution, and the enzyme is immobilized in each fractionation chamber 3 so as to leave a space 4 above. It is filled with an aggregate of fibers 5, and has a substrate solution population 6 at the upper end and a reaction product output lower end at the lower end. Thus, the space agitates the flow of substrate solution from one fractionation chamber to the next, effectively preventing the occurrence of preferential flow. If necessary, one or more baffle plates 8 are disposed in the space (only some of which are shown) in order to enhance the stirring effect of the substrate solution flow through the space.

532図は竪型反応器の別の実施例を示し、容器1は多
孔体セパレータ2により前記と同様に複数の分画室3に
分画され、これら分画室の一つおきに酵素固定化繊維の
集合体5が充填されて、一つの分画室が一つの空間4を
なして集合体を相互に分離している。このような反応器
は横型反応器としても用いることかできる。
Fig. 532 shows another embodiment of a vertical reactor, in which a container 1 is divided into a plurality of fractionation chambers 3 by a porous separator 2 in the same manner as described above, and enzyme-immobilized fibers are placed in every other fractionation chamber. The aggregates 5 are filled so that a compartment forms a space 4 separating the aggregates from each other. Such a reactor can also be used as a horizontal reactor.

第3図は横型反応器の別の一実施例を示し、容器1は前
記同様に多孔性のセパレータ2にて複数の分画室3に分
画され、各分画室にはその上流側に前記邪魔板に相当し
て堰9が設けられて、酵素固定化繊維の集合体5が基質
溶液の流れ方向に相互に分離して充填されている。各セ
パレータと堰との間に空間4が形成される。
FIG. 3 shows another embodiment of a horizontal reactor, in which a container 1 is divided into a plurality of fractionation chambers 3 by a porous separator 2 in the same manner as described above, and each fractionation chamber has the above-mentioned obstruction on its upstream side. A weir 9 is provided corresponding to the plate, and the aggregates 5 of enzyme-immobilized fibers are packed so as to be separated from each other in the flow direction of the substrate solution. A space 4 is formed between each separator and the weir.

このように、本発明の酵素反応器によれば、先ず酵素が
表面積の大きい繊維状担体に固定化され、このような酵
素固定化繊維の集合体を用いるため、従来のビーズ状や
膜状の固定化#素と異なり、反応器活性を著しく高(す
ることができると共に、基質溶液を反応器内に流通させ
る場合にも固定化酵素が圧密化や目詰まりを起こさない
ので、高い反応器活性を長期にわたって維持することが
できる。
As described above, according to the enzyme reactor of the present invention, the enzyme is first immobilized on a fibrous carrier with a large surface area, and since an aggregate of such enzyme-immobilized fibers is used, conventional bead-like or membrane-like Unlike immobilized enzymes, it is possible to significantly increase the reactor activity, and the immobilized enzyme does not cause compaction or clogging when the substrate solution is passed through the reactor, resulting in high reactor activity. can be maintained over a long period of time.

更に、本発明においては、第4図に示すように、各分画
室3を単位円筒容器7で形成し、セパレータ2を介して
複数の分画室を分解自在に組立てて反応容器を形成すれ
ば、容器内の一部の酵素固定化繊維の集合体5、例えば
酵素活性が早期に低下しやすい上流側の集合体を必要に
応じて選択的に交換することができ、運転コストを削減
することができる利点もある。
Furthermore, in the present invention, as shown in FIG. 4, if each fractionation chamber 3 is formed by a unit cylindrical container 7, and a plurality of fractionation chambers are assembled via the separator 2 in a disassembly manner to form a reaction container, Some of the aggregates 5 of enzyme-immobilized fibers in the container, for example, the aggregates on the upstream side where enzyme activity tends to decrease early, can be selectively replaced as necessary, reducing operating costs. There are some advantages to doing so.

【図面の簡単な説明】[Brief explanation of drawings]

第1図及び第2図は本発明による竪型の酵素反応器の実
施例を示す断面図、第3図は横型の反応器の実施例を示
す断面図、第4図は組立自在の反応器の要部断面図であ
る。 1・・・容器、2・・・セパレータ、3・・・分画室、
4・・・空間、5・・・酵素固定化繊維集合体、7・・
・単位容器。
FIGS. 1 and 2 are cross-sectional views showing an embodiment of a vertical enzyme reactor according to the present invention, FIG. 3 is a cross-sectional view showing an example of a horizontal reactor, and FIG. 4 is a freely assembleable reactor. FIG. 1... Container, 2... Separator, 3... Fractionation chamber,
4... Space, 5... Enzyme immobilized fiber aggregate, 7...
・Unit container.

Claims (1)

【特許請求の範囲】 +11  !!に膜状担体に酵素が固定化された酵素固
定化繊維からなる集合体が基質溶液の流れ方向に間隔を
おいて分離して容器内に配設されている、  蚤 ことを特徴とする固定化酵素反応器。
[Claims] +11! ! Immobilization characterized in that aggregates of enzyme-immobilized fibers in which enzymes are immobilized on membrane-like carriers are arranged in a container separated at intervals in the flow direction of the substrate solution. Enzyme reactor.
JP19501981A 1981-12-02 1981-12-02 immobilized enzyme reactor Expired JPS6025112B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP19501981A JPS6025112B2 (en) 1981-12-02 1981-12-02 immobilized enzyme reactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP19501981A JPS6025112B2 (en) 1981-12-02 1981-12-02 immobilized enzyme reactor

Publications (2)

Publication Number Publication Date
JPS5898084A true JPS5898084A (en) 1983-06-10
JPS6025112B2 JPS6025112B2 (en) 1985-06-17

Family

ID=16334176

Family Applications (1)

Application Number Title Priority Date Filing Date
JP19501981A Expired JPS6025112B2 (en) 1981-12-02 1981-12-02 immobilized enzyme reactor

Country Status (1)

Country Link
JP (1) JPS6025112B2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61100187A (en) * 1984-10-22 1986-05-19 Shimadzu Corp Immobilized enzyme column
US4789634A (en) * 1985-06-18 1988-12-06 Dr. Muller-Lierheim Kg Biologische Laboratorien Carrier for the cultivation of human and/or animal cells in a fermenter
JPH01215277A (en) * 1988-02-23 1989-08-29 Fukuoka Pref Gov Rotary drum bioreactor having separated chambers
JPH0686639A (en) * 1992-04-09 1994-03-29 Soc Prod Nestle Sa Method and device for hydrolysis of protein enzyme
JP2014140822A (en) * 2013-01-24 2014-08-07 Akechi Ceramics Co Ltd Sterilization filter

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0158904U (en) * 1987-10-07 1989-04-13

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61100187A (en) * 1984-10-22 1986-05-19 Shimadzu Corp Immobilized enzyme column
US4789634A (en) * 1985-06-18 1988-12-06 Dr. Muller-Lierheim Kg Biologische Laboratorien Carrier for the cultivation of human and/or animal cells in a fermenter
JPH01215277A (en) * 1988-02-23 1989-08-29 Fukuoka Pref Gov Rotary drum bioreactor having separated chambers
JPH0686639A (en) * 1992-04-09 1994-03-29 Soc Prod Nestle Sa Method and device for hydrolysis of protein enzyme
JP2788837B2 (en) * 1992-04-09 1998-08-20 ソシエテ デ プロデユイ ネツスル ソシエテ アノニム Method and apparatus for enzymatic hydrolysis of protein
JP2014140822A (en) * 2013-01-24 2014-08-07 Akechi Ceramics Co Ltd Sterilization filter

Also Published As

Publication number Publication date
JPS6025112B2 (en) 1985-06-17

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