JPS5885806A - Plant growth regulator - Google Patents

Abstract

PURPOSE:A plant growth regulator of a terpene glycoside that shows activities of growth stimulation, blooming and dormancy breaking, which gibberellin has, and causes no yellowing as well as root growth inhibition, futher being stable, soluble in water, readily and inexpensively available. CONSTITUTION:The objective plant growth regulator contains, as an active ingredient, a glycoside containing stevoil as an aglicone, preferably a diterpene glycoside of the formula (R1 is H, metal ion and R2 is beta-glucopyranosyl, or R1 is beta-glucopyranosyl and R2 is beta-glucopyranosyl or R1 is beta-glucopyranosyl and R2 is H) such as 13-O-beta-glucosylsteviol. USE:It can be applicable to grape, persimmon, strawberry, honewort, butterbur, celery, tomato, cucumber, tulip, blettila and hinoki cypress.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a plant growth regulator containing at least one diterpene glycoside exhibiting diberin-like activity as an active ingredient.

Many gibberellins are known to exhibit cihererin-like activity, including gibberellin A8. A7. A3o.

A3□ is well known for its strong activity, and gibberellin A3 in particular is in practical use. However, although these compounds have the property of promoting plant elongation, they also have drawbacks such as causing starvation at practical concentrations, inhibiting root development, and being poorly soluble in water, making them ineffective. It is often pointed out that enough is enough.

As a result of extensive research, the present inventors have found that gibberellins have physiological effects: 1) Growth promoting effect, 2) Effect on flowering, 8) Effect on breaking dormancy and germination, 4) Effect on promoting parthenocarpy. 5) In addition to exhibiting activities such as activating and converting hydrolytic enzymes, unlike gibberellins, it does not cause anemia or inhibit root growth, is physically stable, water-soluble, and easily available. We also succeeded in developing an inexpensive plant growth regulator. The active ingredient is a diterpene glycoside. The effectiveness of such glycosides is due to the fact that gibberellin glycosides are mostly inactive (Crozier A,
C1C0Kuo, R8C, Durl ey st
RoP, Pharis; Can, J., Bot;
.

48 867 (1970) E. et al. et al. suggested that the introduction of a gelcopifusyl group into gibberellins is related to the gibberellin inactivation mechanism.

Thus, the present invention provides formula (■): [wherein Ro is a hydrogen atom or a metal ion, and R2 is β-
Curcobyranosyl i: R1 is a β-glucopyranosyl group, R2 is a β-glucopyranosyl group; 1dR1 is a β-glucopyranosyl group, and R2 is hydrogen. ] The present invention provides a plant growth regulator containing at least one of the diterpene glycosides shown below as an active ingredient.

The above-mentioned Niyama compound is substantially a known substance and can be obtained by a known method.

That is, plants containing glycosides with steviol as the true aglycone, such as Steviol, Nutevia (Stervia), etc.
rebaudiana), or by partially hydrolyzing the isolated product or converting it into a salt according to a conventional method [References: (1) H, Bow) od, R, A11erton,
HWDiehland, HoG, Fl-etcher,
J, Org, Chern, 2. ,．． 0,875
(195j).

(21H, Kohda, R, Kasai, Yama
saki, ni0Murakami, 00Tanaka,
Phytochanistry, 15, ggl
(1R76).

(31M, Kobayasbi, S, l[-br
ikawa, I., H., Degrandi, J.
.

Veno, HoMitsuHashi, Ph
ytochemi 5try, l 6.14 Q
5 (1977).

(4) 1. , Sakamoto, ni, Yamasa
ki, O, Tanaka, Chem.

Pharm, Bull, 25. 844 (1
977).

(5) Toyoshige Morita, Isao Fujita, Jun Iwamura-9 Patent Publication, 1972-27226゜ (6) Junko Ueno, Hiroshi Mitsuhashi, Tetsuya Sumita 9 Collected Abstracts of the 96th Meeting of the Pharmaceutical Society of Japan, Volume 2, 254 (1976) ,
) To give an example of extraction and isolation from Nutevia leaves, dry leaves are first subjected to hot water extraction and extracted with a polar solvent (e.g. methanol).
Next, the above extract is passed through a water-washed resin having a molecular sieving effect (e.g., Amberly XAD-2), and then water is passed to desalt or elute low-molecular-weight compounds. elute. When this solution M is concentrated, a white or pale yellow powder is obtained.

Various diterpene glycosides can be isolated by subjecting this to recrystallization or column chromatography.

A compound of the lfc formula (Il in which 0 is a hydrogen atom) can be converted into a corresponding salt (particularly a metal salt) by a conventional method. Preferred salts include alkali metal salts such as sodium salts, potassium salts, and lithium salts. and these are water-soluble.Other examples of salts include alkaline earth metal salts such as calcium salts and magnesium salts.

The active ingredients of the present invention may be one type or a mixture of two or more types. A particularly preferred active ingredient is [,1B-
0-β-g is the sodium or potassium salt of cosylnutepiol [formula (in which 0 is a hydrogen atom and R2 is a β-glucopyranosyl group]), demonoglucosylstevioside [formula (I) in which R1 is β-glucopyranosyl group] -glucopyranosyl group in which R2 is a β-glucopyranosyl group].

The active ingredient of the present invention is usually used as an aqueous solution. The active ingredient may be prepared in advance as a powder or granules using a solid excipient, and then may be temporarily prepared as an aqueous solution. Alternatively, it may be prepared as a relatively highly concentrated aqueous solution and diluted at the time of use. Water alone is usually sufficient to form an aqueous solution. However, in some cases (for example, when the active ingredient is poorly soluble in water) a suitable surfactant may be added to form an aqueous suspension or dispersion.

The concentration of the active ingredient during use is 0.001 to 0.2
Weight percent is preferred.

Further, the plant growth regulating agent 1 of the present invention may contain other compounds having a plant growth regulating effect (for example, gibberellin) and a nutrient source for plants.

The preparation thus obtained exhibits an excellent growth regulating effect on plants.

It is preferable to use it in accordance with that for gibberellin preparations.

Further, target plants to which the plant growth regulator of the present invention is applied are not particularly limited, but specifically, the following agricultural plants can be mentioned.

i.e. fruits such as grapes, oysters, strawberries;
Vegetables such as honeysuckle, spinach, celery - 1 fuki, spring noodles, tomatoes, cucumbers, eggplants; cyclamen, primula,
These include petal plants such as Miyakowa clover, summer chrysanthemum, chrysanthemum, chrysanthemum, tulip, and terrapin lily, and trees such as pickled cedar and cypress.

In addition, the purposes of use of the plant growth regulator of the present invention include making fruits seedless, accelerating ripening, enlarging fruit grains, promoting fruit cluster elongation, preventing fruit drop, and increasing the number of fruit set; promoting growth and enlargement of vegetables. promotion, growth promotion by breaking dormancy, prevention of hollow fruit, fruit enlargement, increase in number of fruit set, etc.; promotion of flowering of petal plants, promotion of plant height elongation,
Examples include breaking dormancy; and promoting flower bud differentiation in trees.

In particular, the following uses can be mentioned:

(1) Use to adjust the harvest time and ripening period of citrus fruits and other fruits and vegetables.

(2) Applications for promoting the growth of vegetables that rely on greenhouse horticulture such as gravel cultivation and hydroponics.

(3) Use as a flowering regulator for petal garden plants.

(4) Use for growth regulation in raising seedlings of plants of the Senbonaceae family, especially rice (adaptation to mechanical seedling raising).

(5) Use as a weeding aid for weeding by causing weeds to germinate all at once due to the awakening effect of seed dormancy, and then weeding using a herbicide.

Next, the present invention will be explained with reference to Examples.

Example 1 (growth promoting effect) 100.200.500 and ioo of each compound in Table 1
.

An aqueous solution with a concentration of PPm was prepared. 50-sanf.

Place a sheet of paper with the same diameter in a bottle (φB2111+1Xh65M), and place Tanginhose (a rice variety with a dry taste) on top of it.
Oryza 5aliva L+var Tangin
A total of 100 seeds of Bozu (Japanese bozu) were lined up, 2 doses of each of the above aqueous solutions were administered to them, and the seeds were heated at 25°C at about 300°C.
The cells were grown for 14 days in a thermostat at 0/ux. Water was supplied with distilled water ad libitum during this period. The growth status was determined by measuring the height of the leaf and stem, and the results are shown in Table 1.

As a result of macroscopic observation of the tangine pose in Example 1, no depletion phenomenon observed with gibberellin preparations was observed at each concentration of any of the glycosides, and no significant difference was observed from the control group. Yo.

Further, Table 2 shows the results of observing the effect on rooting in Example 1 according to root length. As is clear from the results, inhibition of rooting as seen with gibberellin preparations was not observed.

Claims (1)

[Scope of Claims] A plant growth regulator containing at least one type of glycoside having 17 nuteviol as a true aglycone as an active ingredient. 2. The glycoside has the formula (■): [In the formula, R1 is a hydrogen atom or a metal ion, and R2 is β-
Glucopyranosyl group; R1 is a β-glucopyranosyl group, R2 is a β-glucopyranosyl group; or R1 is a β-glucopyranosyl group, and R2 is hydrogen. ] Claim 1 is a diterpene glycoside represented by
The plant growth regulator described in Section 1. 8. The plant growth regulator according to claim 2, wherein R1 in formula (11) is a hydrogen atom, sodium atom, or potassium atom, and R2 is a β-glucopyranosyl group.