JPS5874615A - Composition for cancer treatment - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to cancer therapy, and more particularly to compositions and methods for cancer therapy that are useful in regressing carcinomas at all stages of development.

In recent years, cancer has been treated with radiation, small amounts of toxic chemicals, viruses,
Two random effects on the transcription of genetic messages in Yikyusen cells.
I became completely aware that an error can be a trigger, starting with every single one of the countless cells that make up the body.

When an external or internal influence alters the genetic message of a cell, the cell begins to divide, defying its normal genetic constraints and forming abnormal cells. Meanwhile, the body's immune system, which is normally on the lookout for foreign cells of all types, does not respond extensively), and its defense units halt their destructive activities during attacks on invading cells. After the damage has been repaired, reproductive cells cease to develop normally, and unlike healthy cells, abnormal cells (cancer cells) continue to grow over a wide range of areas and increase in size because their division regions do not have boundaries. While forming a tumor, the tumor expands into healthy tissue and competes with normal cells for nutrients. These malignant cells also move into the bloodstream or the lymphatic system, and from there are carried throughout the body, creating what is called a metastasis.

Those working in the technology field usually feel strongly that the genetic messages in carbonized cells can be reversed and the formation of cancer can be prevented. But to date, no means have been devised for carrying out such restorations. Therefore, the most commonly used and considered best cancer treatments are unpleasant, debilitating and sometimes disfiguring treatments, the triple combination of surgery, radiation and chemotherapy; Despite these treatments commonly given to cancer patients, two-thirds of all cancer patients eventually die from the disease. I'm here.

The cancer therapeutic agent (cancer therapeutic composition) provided by the present invention is effective against most types of cancer, is effective against any developmental stage of cancer, and has a direct effect on genetic messages in cells. It is obtained from abundant raw materials that are available in large quantities, and is useful as a radical treatment for cancer.

The present invention also provides methods that can effectively treat the majority of cases of cancer at all stages of development.

To describe the invention more specifically, an appropriate daily dose of 4
OF, 1, where cancer is treated at any stage of development by oral administration of 1tK for 60 days starting from day s.
lot@in.); or a suspension of the drug, preferably administered in alternation with an oral dose of immunogenic purine (gamma purine).

It is believed that the invention will be better understood from the detailed description of specific embodiments that follow. These are given merely as examples and are not intended to be conclusive.In recent years, the fundamental properties of living cells have been shown to be the ability of a cell's genes to be turned on or off in response to extracellular signals. It has been studied that it is the ability to For example, 11 In the human body, all cells (
Cells (with the exception of some types of cells such as red blood cells) have the same set of genes, but because the early-stage Ka gene is selected by flipping a switch, cells have different shapes and functions. .

Through research on gene regulation in bacteria and viruses, we have established Fundamental Mechanisms of Gene Regulation, Inc.]) 1M
In recent years, it has been studied that this depends on the interaction of a protein molecule at a specific site 1'C of a long-chain molecule. As a result of this interaction, the gene is switched on and off. In the best-understood example, the gene was turned off by a human controlling a molecule called pretH-regulated: Rp-002. The existence of repressors, 1960 at the Pasteur Institute Q?
7iauioisha sob and raequ・-MO! l
This is the first time that ea has been introduced as a hypothesis. 7 pm 1
(Wait・rGixb・rt succeeded him and worked individually at Barbard College. 9BennoMu41sr-H1
With the help of ll and PtashneO, we isolated the repressor from bacteria ◎ (Mar-ku I'ta@hn*
, Waiter Gllbert arrived [G・n・to
o l@pr --- 0ral (see June 1970 issue) 0Then, the repressor can be tightly linked to the part of the DM system called the operator, and in this way the gene adjacent to the repressor operator It has been revealed that this protein inhibits transcription and translation into proteins.

Baku? Similarly to 97, in humans, the gene is the DM molecule Kl.
It can be defined as a unique base sequence. I) Lim Molecule Co., Ltd., is wound into a double helix and consists of two long chains of nine nucleotides connected to each other by hydrogen bonds. Each nucleotide has a deoxyribose sugar, a phosphate group and four diagonal groups, namely adenine, guanine, helical, and cytosine (
Abbreviation Hiromu, G%! , C) @sugar okarasu.

The acid groups form the backbone of each chain, and the bases extend into the solid axis of the double helix, forming pairs with bases extending from other O chains.
This is a supplementary base sequence line along the chain.

Muhiro always pairs with Te, and 0 always pairs with C. ◎ The information content of DM Mu is determined by the base sequence ↓. A typical gene consists of approximately 1,000 base pairs ◎ Translation from a gene to a protein is performed by the enzyme RM polymethase, which converts the base sequence into [
The transcription of the Metzenger JRN module into the complementary sequence of the linear molecule begins. Messenger R adheres to the intracellular translation machinery called the ribosome.
Translates the NA base sequence into an amino acid sequence (which is linked to form a protein molecule).

It is clear that the translation of genes into protein molecules is suppressed or blocked by one of several steps in a complex gene. It can be concluded that genetic information is not transcribed into the Messenger JItM system as long as the repressor is activated.

Repressors are usually small acidic proteins produced by a gene called the DM regulator, which attaches to the K11 promoter of the gene's operator. The above-mentioned outline of the basic mechanism of transcriptional suppression of the gene by adhering to a part of the goomotor and collecting Metsuryonger R11Al has been described. Although the applicant is not bound by the theoretical explanation of the composition of the induced IjI, it is not good for cancer. is a chemical change in the sequence order of the nucleotides e adenine, guanine, thymine, and cytosine in the gene encoding the synthesis of a specific protein that controls cell o*- and acts as a repressor of the operator gene. It is said that this change is caused by physical, chemical, or biological factors within the cell, and the deformation is transmitted to daughter cells as genetics.Daughter cells that cannot properly synthesize the repressor are There, cells proliferate over a wide area to form an abnormal tissue 0@l) (initial tumor), and the mass metastasizes and spreads to other organs.

In normal cells, a specific repressor protein (Hiro) attaches to the I)N membrane located in the pull motor of the gene operator and visits the transcription of structural genes that control the development and synthesis of the RN membrane. Let's go.

On the other hand, there is a specific protein that is attached to the cell cytoplasm and is attached to the cell INK. These cells are sensitive to cell-cell contact and record empty spaces in the tissue, possibly through electromagnetic changes. These receptor proteins move toward the nucleus of the cell and attach to the regulator gene in the DM, 411, controlling the organism's cell population and inhibiting cell replication without the synthesis of prefusor proteins. provoke.

In cancer cells, the repressor protein, which is influenced by changes in the regulator gene, is altered and does not function, or the repressor protein is deprived. Therefore, cancer cells are in a state where their structural genes are constantly being transcribed, resulting in endless replication.

The susceptibility of a cell to cancer is greatly affected by the increased permeability of the cell membrane. If this cell membrane is highly permeable, physical, typographical, or biological factors that cause cancer to progress may become DI.
0ζThus, certain hormones can influence the rate of enzyme catalysis and alter cell membrane O permeability.This situation is particularly important for tissues that are constantly exposed to chilsen stimulation. For example, it makes the humble offspring of mammals more susceptible to cancer.

The activity of viruses that form tumors! Malnutrition is also a contributing factor to increased cell membrane permeability, as well as decreased immune capacity, which is a contributing factor.The cytoplasm of all living tissue cells contains large numbers of receptor protein molecules. It was confirmed. This white matter is probably generated by electromagnetic or electrochemical changes,
As the cell searches for empty spaces in the adjacent tissue where it is needed, it migrates to the nucleus of the cell and attaches to the DI region's regulator gene. % - Stops producing repressor proteins that block part of the body.

Thus, some of the pull motors are released from inhibition, allowing the operator genes to initiate messenger RNA formation and cell proliferation to fill the empty spaces in the tissue. This action is reversed when the receptors sense the space filled by new cells. This mechanism allows pre-defined cells to multiply only when needed, N1 cells, and proliferation required by the organism. If not, it remains dormant.

When cell permeability increases, external agents that form cysts, mainly viral 1) ml, can penetrate the cell membrane,
It migrates to the nucleus of the cell and becomes permanently attached to the repressor protein. This also causes the repressor gene to no longer produce the appropriate repressor protein. Continuous reproduction of cells begins from the deletion,
The cells become malignant. Therefore, if the repressor protein contained in the tissue is administered to the patient, this repressor protein becomes a substitute for the prefusor protein (which is no longer produced by the malignant cells), and the operator gene It is clear that it acts directly on the motor part and prevents the operator gene from starting the constant reproduction of malignant cells.

It has been discovered that the nervous tissue of mammals contains large amounts of repressor proteins in cells that never proliferate during the lifetime of the individual.90 When these repressor proteins are administered to a patient, they are from the lymphatic and blood systems to cancer-affected cells. 1 The cell membrane permeability of these cancer cells is extremely high, and the administered repressor protein penetrates the membrane and increases the DM of the cells.
It attaches to a portion of the promoter gene of the regulator gene of malignant cells and acts as a substitute Bo repressor molecule for the normal repressor molecule that is not produced by the regulator gene of malignant cells.

For the reasons mentioned above, it has been discovered that DNA obtained from fresh mammalian nervous tissue, especially brain tissue, can be formed in a colloid of IJ-pusser protein and ultimately cause the tumor to regress and disappear.

This type of repressor protein provided by the composition of the present discovery acts not only within the 11IlIII4 but also through the mammary ducts and blood vessels to attack metastatic cells and cause cancer in cancer-affected patients. Prevent metastasis.

To prepare a neural tissue composition in accordance with the present invention, fresh neural tissue from a sacrificed mammal is injected into an aqueous iodine solution (
1:2 SGO) for about 1 to 3 minutes to sterilize and disinfect the tissue, removing living bacteria and preventing other pathogens from entering. Fresh tissue was removed from the soda solution,
Thoroughly wash the tissue with distilled water to wash away the absorbed proteins.Then, wash the tissue mentioned above with something like pre-goo for less than 1 minute to make a colloid layer.
The suspension is stored in a frozen state. This is because the pharmacological action depends on the intact protein contained in the suspension, and this protein is sensitive to high temperatures.

To administer the composition of the invention, the suspension is thawed and administered orally to a cancer patient. Dosage form patient tumor group 1 & 1 ftl
For F, the period of OOS to 1 omes □ is preferably 4s to 60 days ◎ Afterwards, significant regression and final healing were observed in most cases.

The following examples illustrate the efficacy of the cancer therapeutic agent (cancer therapeutic composition) of the present invention to which the above-described cancer therapeutic method is applied.

Example 1 In order to induce adenocarcinoma in mammalian glands, No. 1 muster (
Hamst@r) Cancer cells were injected into a group of 20 people. The cancer was allowed to progress to intermediate stage. When the adenocarcinoma was sufficiently advanced in all 20 selected hamsters,
6 doses of K1mA for 24 hours for 30 days, approximately 5941 ml per hamster I, were treated in 10 days using a composition prepared by sterilizing and sterilizing the brain tissue of cows that had just been slaughtered. Corresponds to a ring. Immune Guprin (Gan! Guppurin) is not used to treat hamsters.

The 10-year-old hamsters selected for treatment were closely observed, and at the end of its day after the start of treatment, the koji tumor tissue had decreased and regressed. Thirty days after treatment, the adenocarcinoma completely disappeared.
The hamster's weight increased by two and nine. After treatment, the patient made a full recovery within 30 days.

We also observed 10 cases of cancer that did not respond to the above treatment.In all cases, without exception, the size of the tumor steadily increased, the vital characteristics were lost, and the body weight decreased by about 2011cm.

All of these hamsters were I died of paralysis.

Example 2 The procedure of Example 10 was repeated, but using freshly slaughtered horse brain tissue. The results obtained are the same as in Example 1. Case 3: A group of 30 volunteer patients with cancer that had fully developed and metastasized to various organs was treated with the composition prepared as follows and slaughtered for 90 days. A piece of fresh cow/horse (lamb) brain tissue was soaked in cold solution (1:25OO) for 2 minutes. The sterilized tissue is thoroughly washed with distilled water and then powdered in a Waring blender for 1 minute to a heavy syrup consistency.

The 30 selected patients were distributed as follows: 9012
Some patients are treated with pincristin adreensin (ma1nor).
He had undergone one round of chemotherapy, being prescribed istin@dreamiains) and cyclophosphoamides. The other 15 patients had already received two to three chemotherapy treatments and several radiation treatments. The remaining three had already undergone numerous chemotherapy and radiation treatments, and vital organs such as the liver, lungs, kidneys and intestines were affected by very advanced cancer.

All 30 patients received the above composition at a daily dose of approximately 1 mW.
/tumor tissue C1F (dose determined for each patient). Human immunoglobulin (gamma globulin) is administered parenterally every 72 hours (1 mj for 3 doses), or equine immunoglobulin (gamma globulin) is administered as a suppository (xomro suppository form M) for 12 hours every 24 hours.
The drug was administered for several days as a way to protect patients from reinfection with the virus.

All patients also received 400 mf t1 lucium phosphate pills three times a day during the gamma gopurin dosing period.

The results obtained from the above treatment are as follows:

The 12 patients who had received chemotherapy only once had their tumor tissue completely disappeared and were on their way to recovery. After one year of management after the completion of the treatment, a tumor tissue indicator, i.e. breast cancer, was administered to the 12 patients mentioned above. #IIl! Manage to F and circle! None of the 91 patients who had already received 2-3 rounds of chemotherapy and several rounds of radiotherapy had satisfactory treatment results. Although the amount of cancer decreased in some cases, this treatment did not completely save the patient from cancer.These patients will continue to receive chemotherapy and radiotherapy after the above treatment. Three patients suffering from cancer received treatment according to the present invention, but all of them died without satisfactory results.

Above all, this treatment is suitable for advanced stages (within certain limits) and
Regardless of metastases, chemotherapy or radiotherapy is highly effective against nine cancers; This indicates that they are more susceptible to virus infection. Viral infection is thought to greatly influence cancer progression through changes in genetic messages in patients' cells.Of the 030 cases, only 12 cases were satisfactorily cured and were completely freed from cancer. This suggests that the treatment performed in Jin's case uses brain tissue from cows and horses, and in at least 40 cases.
- means that it has an effect on O Example 4 Another voluntary group of 20 patients, 10 of whom are 2
She has not received more than one round of chemotherapy and has not received any radiation therapy. Another 10 patients were treated with 8 extensive chemotherapy, radiotherapy and 3 similar treatments.As a result, 19 patients were completely rescued from cancer, and during the management period that was carried out on the aforementioned patients for 1 year after the treatment, tumor tissue was removed. Indication *a It was within the normal level. This means that the patient has successfully recovered from cancer. Only nine people had to undergo remedial treatment. The regression of this patient's tumor tissue was satisfactory after the first treatment, but it was not sufficient to completely prevent all cancer cells, and furthermore, the tumor growth caused by the viral infection. has been revived. The patient was then given a second treatment as described above. This patient has been under follow-up to date, and the outcome is +1+ satisfactory, but the results are 1*+t+.

Thus, the treatment performed according to this example showed a good result of 95-.

The above examples give some hints as to the efficacy of the compositions of the invention. Tsutushi, hamster (H&mat@
r) Treatment of cows with Kuhiro horse brain tissue resulted in 9% 100%
The success rate was 9G. In contrast, when humans were treated with gamma globulin and cartacum phosphate, only 40 cases were considered completely satisfactory. However, when using the brain tissue of a primate, an animal close to human 1 [IK], the reduction rate rapidly increased to about 95.

The implication of the above is that, once legal and ethical hurdles are removed, the use of human brain tissue would probably not be possible.
o110 It means achieving success. But human O
Experiments using the i1 organization will not be conducted. In particular, oral ingestion of human brain tissue would be ethically prohibited, and parenteral administration would require freeze-drying the tissue.

However, since animals are similar to humans, it is true that the effects of their brain tissue can be effective in mitigating the progression of cancer.

The above is apparently due to the fact that the repressor proteins contained in the brain tissues of species close to humans are similar to the repressors in humans that are affected by the development of cancer cells. As a result, the animal's repressor protein acts as a perfect substitute for the animal's repressor protein, which is at risk of being lost or altered by cancer cells, causing turtles to become unborn children.

The outline of the administration method for the composition of the present invention is as follows.

1. DI obtained from subdivided mammalian nerve tissue
Muripressor White matter O sulfur quencher 11 and 9 suspension @ 02
, Method 03 according to the previous paragraph 1, wherein the suspension of the DM mulipreneurial white matter is 0.5 to io mt per 1 mW of the weight of the malignant tumor tissue detected in the patient; 4. Method 0 according to the preceding paragraph 1, in which the brain tissue of an animal is the brain tissue of a bovine.
How to do it depending on the section.

S. The method according to the preceding paragraph 1, wherein the mammalian brain tissue is an avian brain tissue.

6. The above-mentioned mammalian brain tissue is a presuspension of primate brain tissue, and the sterilized col suspension of mammalian whole brain tissue is freeze-dried. 1. The method according to the preceding paragraph containing an id suspension.

9. First, the mammalian brain tissue mentioned above is human brain tissue.
Method by term.

Applicant Daniel Marvandler Torre representative Ueno Ito Yoshimi Yokoyama

Claims (1)

[Claims] (7) The composition according to claim 1, wherein the DM murepressor protein is obtained from mammalian nervous tissue and is produced by Marugame. 2. The composition according to claim 2, wherein the mammalian nervous tissue is mammalian brain tissue. (2) The composition according to claim 3, wherein the mammalian O brain tissue is bovine O brain tissue. □ 俤) Composition 6 according to claim 3, wherein the mammalian brain tissue is equine brain tissue 俤) Patent claim OSm according to claim 3, wherein the ocelot O brain group 1 is primate ll0mg tissue Composition of. (η) The composition according to claim 3, which contains a colloidal suspension in which the DM murepressor protein is sterilized and sterilized, and the whole tissue of a mammal is sterilized. (2) The composition according to claim 3, wherein the DM murepressor protein is a colloidal suspension of freeze-dried whole brain tissue of a mammal. Composition 0 according to claim 8, which is brain tissue of