FR2481602A1 - COMPOSITION AND METHOD FOR THE TREATMENT OF CANCER - Google Patents

Abstract

COMPOSITION FOR THE TREATMENT OF CANCER, CONSISTING OF A STERILIZED SUSPENSION OF DNA REPRESSOR PROTEINS, FROM A FINELY DIVIDED NERVOUS TISSUE OF MAMMALS. THE COMPOSITION, PREFERRED FROM A HUMAN BRAIN TISSUE OR FROM A MAMMAL CLOSE TO MAN AT THE ZOOLOGICAL SCALE, IS ADMINISTERED ORALLY. APPLICATION TO THE TREATMENT OF CARCINOMATORY DISEASES.

Description

Composition and method for the treatment of cancer

  The present invention refers to the treatment

  of cancer and, more particularly, relates to a

  and a cancer treatment method useful for involution of carcinoma at any stage of its evolution. It has been perfectly admitted in these last

  years, that cancer can start in just one of

  cells, being triggered by a radiation dispersion, a trace of toxic chemical, a virus or a random error in the transcription of the cell's genetic message. Once the genetic message of the cell is altered by some external or internal influence, the cell begins to divide to form others.

  abnormal cells in a process that escapes the restric-

  normal genetic The immune system of the organism

  on the other hand, who is normally vigilant in

  which concerns the presence of all types of foreign cells ,.

  does not respond properly and his defense units are interfering

  say to attack and destroy invasive cells. Unlike healthy cells, which stop their reproduction after repair of damage or contribution to normal growth, aberrant cells (cancer cells) have no limit in relation to the dividing properties

  and continue to proliferate widely, forming a growing mass

  health or tumor that expands into healthy tissue and competes with normal cells for nutrition. The

  malignant cells can also be transferred to the

  blood circulation or the lymphatic system that carries them throughout the body, creating what are known as

metastases.

  Although the specialists of this technique strongly believe that the cancer could be stopped if the altered genetic message of the cell could be restored, no way has been defined so far to achieve this recovery. That's why treatment. cancer, which are considered the best, consist of - 2 -

  to prolong the life of patients during

  years, by one or a combination of three types of treatment, often unpleasant, debilitating and sometimes disfiguring, namely: surgery, radiation and chemotherapy. Despite the existence of these types of treatments usually applied to cancer patients, two-thirds of all cancer victims ultimately die of the disease.

  The present invention provides a composition

  for the treatment of cancer which is useful for most

  cancer types, which can be effective-at any stage of the disease's development, which acts directly on the genetic message of the cells so as to inhibit the reproductive capacity of the cancer cells, and which derives from a starting material abundant, available in large

  amount to serve as a radical cure for cancer.

  The present invention also provides a method of treating cancer that can be effective in curing the disease in a large percentage of cases at any one time.

  stage of the development of this disease.

  According to a preferred embodiment of the present invention, the cancer is cured at any stage of the disease by the method which comprises administering to the patient orally a suitable daily dose, for a duration of about 45 hours. at 60 days, of a composition comprising a colloidal or semi-colloidal suspension of DNA repressor proteins, derived from a fresh mammalian nervous tissue, in particular brain tissue, in

  alternation with parenteral immunoglobulin

lines (gamma globulins).

  The present invention will be better understood

  from the following detailed description of some

  specific modes of implementation which are simply

  illustrative, and without any limiting character.

  In recent years, it has been found that a fundamental property of living cells is their ability to release or block their genes in response to extracellular signals. In the human body, for example, each cell (with the exception of a few cell types such as red blood cells) has the same set of genes, although during embryonic development the cells adopt different forms and functions according to than

  their genes are selectively unlocked and blocked.

  By studying the genetic regulation in bacteria and viruses, it has been learned in recent years that a fundamental mechanism of gene control depends on

  the interaction of protein molecules with specific regions

  of the long-chain DNA molecule. The result of

  this interaction is that the genes are blocked or unblocked.

  In the best understood cases, the genes are blocked by control molecules called repressors. The existence of repressors was first suggested in 1960 by François Jacob and Jacques Monod, of the Institut Pasteur in Paris. Seven years later, Walter Gilbert, in collaboration with Benno

  Muller-Hill and Ptashne, working independently at the University

  Harvard, have succeeded in isolating repressors from bacteria (see "Genetic Repressors", Mark Ptashne and Walter Gilbert, Scientific American, June 1970). Later, it was shown that repressors could bind tightly and specifically to DNA sites called operators, and that in doing so they could prevent adjacent genes

  to the operator to be transcribed and translated into proteins.

  In humans, as in bacteria, a gene can be defined by a sequence of bases along a DNA molecule. The DNA molecule consists of two long

  nucleotide chains coiled into a double helix and

  linked to each other by hydrogen bonds. Each year

  cleotide consists of a deoxyribose sugar, a phosphate group and one of four nitrogenous bases: adenine, guanine, thymine

  or cytosine (abbreviated as A, G, T and C). Sugar and coffee

  Phosphate form the backbone of each chain; the basics

  extend towards the central axis of the double helix and are

  bet with the bases extending from the other chain.

  The base sequences along the chains are complementary: A is always paired with T and G is always paired with C. The information content of the DNA is determined by the sequence of the bases. A typical gene consists of approximately 1,000 base pairs. The translation of genes into proteins begins with the enzyme RNA polymerase, which copies the base sequence into a complementary sequence on the linear molecule of messenger RNA. The intracellular translational means, called ribosomes, bind themselves to the messenger RNA and translate its base sequence into an amino acid sequence, which is

  connected to form a protein molecule.

  It is clearly conceivable that the trans-

  of a gene into a protein molecule can be

  awarded or blocked at any of the different steps along the complex gene. Recent studies have determined that repression takes place directly on the DNA molecule, so that genetic information is not transcribed into

  Messenger RNA as long as the repressor is not inactivated.

  Repressors are generally

  small acid eels, produced by a gene called

  regulator in the DNA, and which settle in the region of

  motor of a gene operator, thereby preventing the poly-

  merase to bind itself to this promoter region and assemble the messenger RNA. After recalling the basic mechanism of transcription repression of the gene, and being specified

  that the applicant does not intend to be bound by an explanation

  theory of the mode of action of the composi-

  of the present invention, it should be made clear that one thinks

  that cancer is the chemical alteration of the order of nucleotides

  tides, adenine, guanine, thymine and cytosine

  gene that regulates cell duplication and codifies the syn-

  thesis of a specific protein that functions as a re--

  pressing of an operator gene. This alteration caused by

  physical, chemical or biological agents in a

  lule, hereditarily transmits this transformation to daughter cells that can not synthesize properly

  the repressor, which causes the wide reproduction of

  lules and forms the abnormal tissue masses (primary tumors

  mayors) that can spread to other organs through metastasis.

  In a normal cell, the protein

  specific pressor adheres to the DNA, as mentioned above, in the promoter region of a gene operator and inhibits

  the transcription of the structural genes that control the

  development and synthesis of RNA.

  On the other hand, there are some protein

  specific receptors arranged in the cytoplasm of

  the cell, attached to the membrane and which are sensitive to

  intercellular tact and record the empty spaces of a

  tissue, probably by electromagnetic

  therefore, cell duplication

  migrate to the nucleus of the cell and adhere to the DNA,

  particularly to the regulatory gene and prevent the synthesis of the repressive protein that controls the cell population

in a living organism.

  The cancer cell is thought to contain an altered repressor protein that does not work or

  that it is simply devoid of this protein in function

  tion of the change that has been caused in the regulatory gene and, as a result, this cell is still in a state of continuous transcription of the structural genes, which is

  translated by an indefinite duplication.

  The susceptibility of a cell to cancer depends greatly on an increase in the permeability of its membrane that allows a physical, chemical or biological cancer development agent to penetrate into contact with its DNA. So, some hormones act in. affecting

  the rate of enzymatic catalysis or altering the

  This circumstance renders these tissues, which are subject to a continuous hormonal stimulus, more prone to cancer, for example the nammary gland and the uterus. A lack of nutrition may, in other circumstances, cause an increase in the permeability of the cell membrane, as well as depression of

  immunocompetence, which are factors that facilitate the

  oncogenic viruses. It has also been established that the cytoplasm

  of each cell in a living tissue contains a multiplicity

  cited molecules of a protein called receptor. This pro-

  probably due to electromagnetic changes

  ticks or electrochemical, detects empty spaces in the fabric

  where cells are requested and, in doing so,

  The receptor protein migrates to the nucleus of the cell, attaching to the DNA regulatory gene which, therefore, will not produce more repressor protein to block the promoter region of the operator gene. As a result, this region of

  promoter will no longer be repressed and will trigger the opera-

  to start messenger RNA training and to reproduce

  duction of the cell to fill the empty space of the tissue.

  This action will be reversed when the receptor proteins no longer detect empty space once it has been filled with new cells. This may be the mechanism by which a predetermined cell reproduces when necessary, and remains at rest from this point of view when the

  reproduction is not requested by the living organism.

  Increasing the permeability of the membrane of a cell may allow oncogenic agents

  external organs, mainly viral DNA, to cross this mem-

  brane and migrate to the nucleus of the cell, attaching

  permanently to the regulatory gene, so that this gene

  gulator will no longer produce the appropriate repressor protein.

  As a result, the continuous reproduction of the cell will be

  triggered by the absence of the repressor protein and the

lule will become malignant.-

  It is clear, therefore, that if a

  A repressor protein contained in a tissue is administered to a patient, this repressor protein may replace the repressor protein which is no longer produced by the malignant cell, and will act directly on the promoter region of the operator gene to prevent this. last to cause continued reproduction

of this cell.

  It has been found that all mammalian nerve tissue, being cells that never reproduce during the life of the individual, contain levels

  of these repressor proteins, so that, when

  they are administered to a patient through the gastrointestinal tract, they are transported by the systems

  lymphatic and bloodstream to the cells affected by the can-

  cer. Since these cells have significantly increased membrane permeability, the administered repressor protein will penetrate these membranes and attach to the promoter region of the DNA operator gene of these cells,

  acting as repressor molecules in place of

  normal repressors that are not produced by the

  regulator gene of the malignant cell.

  That's why the composition

  of a substance containing a colloidal or semi-

  colloidal protein repressor DNA derived from a tissue

  mammalian nervous system and particularly of ceremonial tissue

  bral, has been shown to be extremely active in suppressing and preventing the reproduction of <ancestor

  and finally produce the involution and the disappearance of the

  initially formed by these cells.

  This type of repressor protein provided by the composition of the present invention not only acts within the tumor itself, but also acts through the chyline vessels and blood vessels to attack the metastasized cells. , which also inhibits cancer metastasis in a patient affected by

this pain.

  The composition of the nervous tissue, in accordance

  According to the present invention, it can be prepared by immersing a fresh animal nerve tissue, obtained from a recently slaughtered mammal, in an aqueous solution of iodine (1: 2500) for a period of time of about one hour. to three minutes, so as to sterilize this tissue and to prevent the participation of any live bacteria or other germs. The fresh tissue is removed from the iodine solution and thoroughly washed with distilled water to wash the absorbed iodine and then this tissue is divided, for example into a mixer, for a period of one minute or less, way to form a colloidal mass

  or semi-colloidal which is preserved under refrigerated conditions

  managed because the pharmacological action is highly dependent on the

  of the proteins it contains, these proteins being

  Sensibless at high temperatures.

  To administer the substance composition of the present application, it is thawed and orally administered to the cancer patient, preferably at doses of 0.5 to 10 mg / mg of tumor tissue determined in FIG. patient and for periods of time of about 45 to 60 days,

  after which we observe a remarkable involution and a cure.

  its final disease in a large proportion of cases.

  The following examples will illustrate the effectiveness

  cacity of the composition of the present invention administered

  by the cancer treatment method described above.

Example 1

  A group of twenty hamsters received

  cancer cells to induce adenocarcinoma

  mammary gland and the cancer has been allowed to develop.

  lopper at an intermediate stage. When adenocarcinoma has been fully developed in all twenty chosen hamsters,

  a group of ten of these hamsters was treated with a

  comprising finely divided and sterilized brain tissue of a recently slaughtered bovine animal, and at 1 ml per 24 hours for 30 days, equivalent to

  dose of approximately 5 mg / kg of hamster per day. No immunity

  Globulin (gamma globulin) has not been used in the treatment.

hamsters.

  The group of ten hamsters chosen for the

  treatment was carefully observed and at the end of

  day of the start of treatment, the tumor mass began

  to decrease and to. involuntarily, whereas after 30 days of -9 treatment the complete disappearance of adenocarcinoma has been

  obtained, and the weight of the animals increased by 7%.

  sters having fully recovered by the end of the thirtieth day.

  The group of ten hamsters, which was not treated as above, was also observed. All, with the exception of one, showed a considerable increase in tumor volumes, a loss of vital characteristics and a loss of weight up to about 20%. All these hamsters

  died in the next ninety days.

Example 2

  The procedure of Example 1 was repeated,

  but using brain tissue obtained from an animal

  bad horse recently shot. The results turned out to be

the same as in example 1.

Example 3

  A group of 30 willing human patients

  res, suffering from totally developed and metastasized cancer in different organs, was treated with a composition prepared as follows: a portion of brain tissue obtained from a recently slaughtered bovine animal (lamb) was infused into an iodine solution (1: 2500) for two minutes; the disinfected tissue was thoroughly washed with distilled water and then divided into a waring blender for a period

  of a microchip, to reduce the tissue to the consistency of a

heavy rop.

  The 30 selected patients were divided

  12 of these patients had undergone chemotherapy

  therapy with vincristine adreamicin and cyclophosphamide; 15 other patients had already been treated two or three times with chemotherapy and several times with radiotherapy, and another group of 3 patients had already been treated with a multiplicity of chemotherapeutic and radiotherapy sessions and had a very advanced cancer attacking vital organs such as the liver, lungs, kidney and

the intestine.

  All patients were treated with an approximate daily dose of 1 mg / mg tumor tissue (determined for each patient) of the above composition,

- 10 -

  and they were also given either human immunoglobulin (gamma globulin) at a dose of 1 ml every

  seventy-two hours, parenterally for three

  immunoglobulin (gamma globulin) obtained from the horse, in the form of 1.0 mg suppositories by administering a suppository every twenty-four hours,

  for twelve days, as a means of protecting the

  against viral reinfections.

  Also, it was administered to all patients.

  400 mg calcium phosphate pills, three times a day during the administration period of gamma globulin.

  The results obtained by the treatment

  above are: the 12 patients who had undergone only one chemotherapeutic treatment showed a

  perfect evolution, with complete disappearance of tumoral tissues

  and after one year of control following the end of the

  treatment, the indicators of tumor tissue, namely the

  dehydrogenase and alkaline phosphatase, are

  due to normal values of approximately 193 international units

  nals (IU) for lactic dehydrogenase and about 72 IU for alkaline phosphatase. None of these twelve patients

  showed a tendency towards an increase in the pre-

  cited during the year of close control.

  The 15 patients, who had already undergone two

  or three chemotherapy treatments and several

  by radiotherapy, have not responded satisfactorily

  treatment and, although in some cases

  nomes were decreased in volume, the treatment did not

  completely cured of the disease. These patients had to continue

  to cancel chemotherapeutic and radiotherapy treatments

  after the aforementioned treatment, without any satisfactory result.

  As for the 3 patients who had undergone numerous chemotherapeutic and radiotherapy treatments and had cancer affecting the vital organs, all are

  died despite the treatment of the present invention.

- ill -

  This shows that the treatment is more effective

  when the cancer has not been treated with chemotherapy or radiotherapy, regardless of the stage of development (within reasonable limits) and metastases involved, and that said chemotherapy treatment, by reducing the immunological response of the patients, makes them more susceptible viral infections, and it is thought that viral infections have been

  much influence in the development of cancer by changing

  trusting the genetic message of patients. Of the thirty cases, only twelve were satisfactorily treated and were completely relieved of the disease, which proves that the treatment performed, as described in the example, has been shown to be effective in at least 40% cases, using

  the brain tissue of a bovine animal.

I5 Example 4

  Another group of twenty patients willing

  res, ten of whom had not received more than one chemotherapy

  therapy and no radiotherapy treatment, and of which ten had received several of these treatments (chemotherapy and radiotherapy) were also treated using the same type of treatment described in Example 3, but preparing the composition of the present invention using a brain tissue from primates, including American monkeys,

  with the result that nineteen of the twenty cases were complete-

  the control of these patients, for one year since treatment, have shown that the indicators of tumor tissue have remained in normal levels, which means that these patients have been completely relieved of the disease. of cancer and only one of them had to be subjected to repeated treatment because

  Involution of tumor tissues, although satisfactory during

  that the first treatment had been applied, had not been shown to completely inhibit all cancer cells, and that perhaps the viral conditions affecting this patient after treatment caused a recovery of tumor growth, so that a second treatment

  identical to that described above was applied to it.

-12-

  This patient is under observation at this time, with results

  satisfactory, but still uncertain.

  Thus, this treatment implemented according to

  this example showed 95% of favorable results.

  The examples above provide some

  suggestions as to the effectiveness of the composition and treatment

  With regard to the present invention, in that when treating hamsters with brain tissue of bovine or equine, 100% success was obtained, whereas, in that. humans with certain brain tissues, along with gamma-globulin and calcium phosphate treatments, only 40% of these cases were considered

  entirely satisfactory. However, the proportion of

  significantly increased to around 95%, using

  cerebral tissue of primates, which are animals the

  closer to the human species on the zoological scale.

  This may mean that, while some

  legal barriers and ethical barriers must be overcome,

  to be the use of human brain tissue could allow

  100% success, but no experimentation could be done

  human brain tissue, especially considering that oral ingestion may be rejected for ethical reasons, and that it would be necessary to lyophilize these tissues for administration by the

parenteral.

  However, it's actually that more animal -

  is close to the human species, the more the action of the ceremonial tissues

  braux of these animals is effective in suppressing the development of

- development of cancer.

  This, obviously, may be due to the fact that the repressor proteins contained in the brain tissue of an animal species close to the human species, may be

  closer to the human repressors that are affected when

  that cancer cells are developed, thus acting

  as a perfect substitute for these repressor proteins

* due or altered, which are not. more produced by cancer cells.

- 13 -

Claims (8)

  1) - Composition of substance for the treatment of cancer, characterized in that it comprises a sterile suspension of DNA repressor proteins,   derived from nerve tissue, -finely divided, mammalian.   2) - Composition according to claim 1, characterized in that this mammalian nervous tissue is a mammalian brain tissue.   3) - Composition according to claim 2, characterized in that this mammalian brain tissue is Bovine brain tissue.   4) - Composition according to claim 2, characterized in that this mammalian brain tissue is equine brain tissue.    ) - Composition according to claim 2, characterized in that this mammalian brain tissue is a ovary tissue of a primate.   6) - Composition according to claim 2, characterized in that the sterilized suspension of proteins   DNA repressors include a sterilized suspension   loldale of all the brain tissue of a mammal.   7) - Composition according to claim 2,   characterized in that said sterilized suspension of   DNA repressor tines includes a suspension of everything   the freeze-dried brain tissue of a mammal.   8) - Composition according to claim 7, characterized in that this mammalian brain tissue is human brain tissue.   9) - A method of treating cancer characterized   characterized in that it comprises administering to a patient daily doses of a sterilized suspension of DNA repressor proteins derived from nervous tissue, finely - 14 -   divided, of a mammal, for a period of time from 45 to days, alternating with the administration of gamma globulin   for a period of two or three weeks.    Process according to claim 9, characterized in that the daily dosage of said suspension of DNA repressor proteins is 0.5 to 10 mg / mg.   weight of diseased tumor tissue detected in the patient.   characterized in brain tissue characterized in brain tissue characterized in brain tissue characterized in brain tissue characterized by 11) - The method of claim 9, wherein the mammalian nervous tissue is a of mammal.   The method of claim 9, wherein the mammalian brain tissue is a of bovine.   The method of claim 9, wherein the mammalian brain tissue is an equine. 14) - The method of claim 9, which the mammalian brain tissue is a of primate.    ) - Process according to claim 9, which the sterilized suspension of proteins   the DNA comprises a suspension sterilized col-   loidal of all the brain tissue of a mammal.   ) - Process according to claim 9, characterized in that the sterilized suspension of proteins   DNA repressors include a sterilized suspension   loldale of all the brain tissue, freeze-dried, of a mammal.   1S) - Process according to claim 16, characterized in that the mammalian brain tissue is brain tissue humai.i.