JPS5842970A - Receptacle for blood test - Google Patents

Abstract

PURPOSE:To accelerate the blood coagulation, to improve the separation of serum and blood clot, and to enhance serum yield, by coating the inside of blood test receptacle with polysiloxane and adsor inorganic material, further adding a specified amine compound to the two above-mentioned materials. CONSTITUTION:A coating solution in which polysiloxane polymer and an adsorptive inorganic material such as microparticles of silica are dissolved and dispersed in methanol is coated to the inside of a receptacle for blood test and dried. Said polysiloxane is shown in the formula (R1, R2 is H, alkyl, vinyl, phenyl, alkoxy, alkylamino; X1, X2 is H, alkyl, vinyl, phenyl, alkoxy, OH, alkylamino and NH2; n>=4; carbon atoms of alkyl, alkoxy alkylamino in R1, R2, X1, X2 are preferably 4-20). Hereby, blood coagulation is accelerated and the separation of serum and blood clot is improved. When a water-insoluble amine is further added to the two above-mentioned compounds, the adhesion effect of fibrin and blood clot is moreover improved and the serum yield is enhanced.

Description

[Detailed Description of the Invention] Superposition - relates to a blood test vessel, in detail, it was obtained from a test subject Okinohshui (7 separations, a garden Og-shaped container used for separating serum). , Tokoro ■ Feed Spitz.

Near Tokyo, along with the repeated mistakes in testing techniques, serum biochemical tests and serum immunity 11! Blood tests and blood tests from the blood cell testing department are widely integrated, contributing to the prevention and early diagnosis of diseases.
ing. Serum tests mainly consist of blood tests.
Inspection Kl! Excessive serum is collected in a blood sac test, and the blood clot is coagulated and then centrifuged to form a blood clot with a specific gravity of OA.
Separated by 1sb.

Conventionally, glass 40 and 49 styrene, polymethacrylate V-), polyethylene and other metals have been used as containers for O blood tests, but these generally have the following O drawbacks. Teru.

One is to inject blood into a blood test container, and after that, the blood clot was 11Kj.
It takes about 0 hours to complete the test, so it is important to carry out the test quickly)
! There is a certain pigeon coop that needs to be given, and there is one with Okadai. Genden blood is said to have a short coagulation time, so it is used to test the s5 temperature, and the blood is injected into the t''eK40, which is 11 minutes after the blood clots.
However, synthetic mIII blood test springs require 4 to 60 minutes for blood to clot.
Because it requires a place in 1.

The disadvantage of the conventional OJK test equipment is that the specific gravity of the blood can be reduced to OA by centrifugation or other means such as coagulation and centrifugation.
In addition, when preparing a bottle of pure serum for use in laboratory tests, the separability of serum is generally stable.

In other words, when gel-like fibrin is full, blood clots are strongly attached to the tube wall.
Another problem is that fibrin tends to remain in the serum, and its rounding can cause problems in serum biochemical tests.
VS* exists and is round. And when using 4-Jl *h, the upper 1eO
R11 is being controlled.

The present inventors have studied the structure of substances that have the effect of promoting and promoting the coagulation of the liquid, which eliminates the above-mentioned fire, and have found that the most effective Km In order to do this, we carefully considered the material structure that should be present in the blood test container, and as a result, we decided to use a combination of heavy metal bodies, adsorption materials, and inorganic materials to form a round pigeon coop. It has been found that the serum components and blood clot components can be effectively separated from each other while the serum components and blood clot components can be effectively separated. KM has completed the present invention by discovering that the adhesion of blood clots, etc., to the inside of the container is even more excellent.

Book JI1114ri! Therefore, ζro is L inner wall 1ii
K% C I? A container for blood testing, characterized in that a +y-layered body and an adsorbent inorganic substance are present. Sui, blood test container, K's turn.

Next, the blood test container of the present invention will be explained in more detail. □, 1□ In accordance with the present invention, as the blood test container, that is, the Subinz O material, a flexible resin, an island hardening resin, a modified natural resin, and 119S0-slip t are used. As adhesive resins, examples are Marage Posiethylene, Polygoupyrene, Poly-
4-methylpentene-1, g9 sterey, polymethyl metatarylate, polyvinyl chloride, TIII diethylene tere 7-late, bobbtylene tere 7-late, styrene-ata-9-nitrile copolymer, styrene-masiic anhydride Copolymer metal, styrene-Ata9 acid copolymer, sterene-methyl metatarylate copolymer, ethylene-pyrene copolymer, ethylene-acrylic acid copolymer, ethylene-acrylic acid ester copolymer, polyvinyl Al；′
- Luaceter Enzyme compound, 4911 Lualj- Lupute Tool compound ll1fi is 1* as a thermosetting resin.

□For example, unsaturated polyester resins, epoxy resins, epoxy resins, etc. are used.

Examples of the modified natural resins include acetic acid-kkII+-su, Globion Shun 4j &;

O-J19 gases include soda lime I-TS, phosphoric acid L-TS, borosilicate L-TS, etc.
Tsusu Ebiishi II &s Tsusu is eyebrow V%.

Honzen@ni s? - I am reluctantly present in the IIO, the blood cell examination committee.
As a heavy metal body that can be used to change the life of a metal, one represented by the following formula is suitable for use.

In the above formula K>-, 1 is a hydrogen atom, an alkyl group, a bean group, a 7-l group, an alkyl-V group, and a ruaceno group in alkyl), XhX, is a water volume atoms, alkyl groups,
Byl group, group 0-, ani: IdP group, hydroxyl group, a#
In the middle Lua ζ group, or amino group, Ih10m is the number of oH on 4JIt.

In the above situation Wc5I-1, Mouse 1, Jl's Xi %
x Yuki O alcohol + i1! , Al-Fugo V group, Rua-electronic base line carbon in Al is 4 to 11180% O is suitable), and as an amino group, primary, tertiary, tertiary, quaternary O, any of O - no group Deshima, Te4. good.

As for Otori, glue to l... is suitable.

11C8/II as a heavy metal body, ms, '11m
may be substituted to form a crosslinked product.

It has excellent properties to reduce the hour wheel phenomenon. Teru.

However, since a 1k-sized sheenan polymer does not have a sufficient effect on blood coagulation preservation, a sheenan polymer and an adsorbent inorganic substance are used in combination. The use of V-iron-condensing bodies and persistent inorganic substances not only significantly promotes blood coagulation, but also causes blood coagulation. 10 and 1k) where O adhesion does not occur,
The bottle is extremely suitable for separating serum components and blood clot components, and the vhItk substance is widely used.

The first inorganic substance itself is an inorganic substance that has excellent first effect performance, and when Tome came into contact with Sumeragi and Kei, Obasha was blood clotted.
The Xth Tomi child, called the iI writhing calf Owataria child, is in contact with the Kalita lei y9 and promotes the activation of the contact child O,
In addition, examples of inorganic substances that promote platelet aggregation include glass, silica, kaolin, ryutsuite, ventona 44, etc., and 41, blood coagulation. The nineteenth most effective thing that saves time is silica.

Silica is produced by hydrolyzing silicon chloride in an oxyhydrogen flame and has an average particle size of 5 to 86.
A moldy silicic acid with an l1 value of 2 to 6 tones, Tenso sulfuric acid] The average grain produced is 11!7! F to 10-voice moldy particles, etc. are suitable.

The weight ratio of the adsorbing Ik inorganic material and the above-mentioned t-polymer is 1 part of the adhering inorganic material to 1 part of the inorganic material in the stain.
It is preferable that the value be within the range of 3i11@. ,7
- Adsorptive inorganic substance 04K for Decoration I2 and T-ton double building.

Acenized hardware II! 1) Promotion of blood coagulation and fibrin in blood clots - the effect of preventing adhesion to the inner wall surface is even better, reaching 9% O. To avoid the OII solution, the ninth step is mizufutsu 04
4D is suitable for use), and 1st class Ill slE
4IlAnanichiihiKanamonoOAny of the 101st grade acenated metalmono, 1%O expressed as 1N1m, a19
However, it is an aliphatic group or an unsaturated aliphatic group,
It is more likely to contain an alkaline hydroxyl group, an alkyl hydroxyl group, and an ether bond. Those expressed as 1s4D saturation ea), *戈, Is are saturated 1g% or unsaturated ssm* group groups, but agonal hydroxyl group,
Acano-Kai 3 and Ether-Kosha tII- will be able to meet Esther Clamping. 11, at least one of Im O
Saturation or incapacity, Japanese ** Group basic member bulk is 7 to 1$ O is preferable. One of the alcoholic water and the other has a nide group in the alcoholic water 9 acid, !!11, etc.
(C1 Tomi N ■-0C
I snow seven chlorine Cl 1 m cm snow C 1 m 0), buy (polyethylene o life! ido ethyl) M (ethylene #cystearyl) amine (etc. are suitable values, layer '41, tertiary adenkasha, things Although it is Hirokoji 1m% and Hirofukame egg fat** group, it is heated by ζ and Toga, which have Artzur hydroxyl group, A() ether association ether clamp, and Hiro ester vessel. .14,ll,!
-at least one') e*114L< is not j1111 fat・
The group 11 group has a JL search of 1 to 1, and O is a cape, and 41-suitable materials are
14, ll, Otori・Oshi (tomo = 2 meets the work and the id- in the cloth, so O″e&), more preferably 8” cm
"0)0"-CIImCIItO)all where 14 is a prime number l:no III 10 alkyl group, 0 is any 0** of 1 or more), for example stearylgetanolamine, tsuku! Ludiethanolamine et al. Quaternary amine compounds are saturated or unsaturated aliphatic groups, but they also contain alkyl hydrogen groups, amino bonds, ether associations, and ester bonds. . X is a stomach dan atom. 4
%O suitable for tatami mats as class A ζ annized hardware is li% * Hatake,
' *, 11.0 at least 4 have an ethylene oquinide group, *e at least one has a carbon number of 1 to 40, and Ru.

What is the weight ratio for the engine 0* weight ratio? t 17ki1'7'ollllllF'
! &$7. .

is suitable.

Sea-life polymers, adsorbent materials, or acene compounds 04) To make water present in 1 ml of a blood test container, add a mixture of the above-mentioned components II# or #i.
■Use a nine-dilution box to turbidify the diluted wave in the container.
After spreading, it is preferable to dry the wi1/s to form a film, or to dissolve or cloud the metals of each of the above components and use a diluted container as the spray value to apply the spray. .

Or use sP- for ＼＼＠＠ meeting, and pre-apply tar silane compounds such as Ditarsin, F9, I11#, etc. on the wall inside the container, Sial;
Ah, this is a V-tsun monster, and I'm sorry for my side's life! - etc. and intermittent noodle machines, etc. - Jk cold the dilution wave that meets these and the Ayanka building materials, and cause a heavy reaction in the presence of moisture and turn the stomach ki into a heavy metal body. It is also possible to obtain a 71-like material by placing it on a container aiii from a horizontal object or an aggregation structure.

The above 04) component can be dissolved or dissolved into a diluted wave as a rounded kM%A housing, and can be used in the form of methanol, ethanol, polyvinyl alcohol, tert-butyl alcohol*0 algol, and tertiary butyl alcohol. Form, difluoroethylene, tatami ivy fluoroethylene, difluoroethylene, difluoromethane, etc. are preferably used.

According to Zero Invention Kosho's test weight, the blood clots - four children are quickly El.
As a result, the time required for blood clot 11 is significantly increased, and blood clots in the blood clot 11 are produced.
k), As a result, the separation of the blood clot and the blood clot is carried out in harm 1,
Separation! Damn it, W! The remaining blood clot ingredients are mixed into the S.1. As a result of the sufficiently progressing of the blood clot component O, the appearance of the blood clot becomes noticeably hotter.

In terms of quality, the main menu - blood test layer test - includes blood test blood collection tubes, blood separation tubes, blood collection waste V-rings, serum separation containers, etc.
Appropriate *use for the purpose.

Execution jilt Outside @'17%, Inner side 1s%, Height 11% Poly2 Hiv y IIOJk I [111 Examination M Spring IIOF'l
1ml OjlE If ip et al., 1...%t”
Sea life number policy in ees minute! - as a poly-9 diotatadecylcylinylnan (average molecular weight #4.0) having an ethoxy group at the end of the car and a natural amine lutzas professional acid mold car (average *11 1 turn), convert 4 to 1. Make a coating solution by dispersing both substances in 11ml of methanol K, apply it to 11ml of a blood test container, and add 1x11ml of methanol afterwards.Amount of coating: 1ml of the above polydioctadecyl vaqnan. ×
1・-4,, /1・・-1 The above acoustic acid mold trunk end is λ wisteria x 1・
``lr / t...d, and after massaging, leave it for 24 hours in a year open at ℃, and then blow the breporamer over the top of the container to form a stable film. .

Blood test level 11 (Normal healthy person) Please take a blood sample.
Immediately after blood collection, inject using KICC and leave in snow at ℃.

It takes t''1' when the whole blood stops flowing completely, and it takes 9 hours as blood coagulation time, and immediately after blood coagulation, KIO@a
11th turn/I1 minute O11th turn blocker performs easy threshold arresting separation, and while observing the Emperor's position - 〇 condition, pump the upper R fist serum with a pipette, and take the amount of O as the amount of serum.

Table 10111111 examples! As is clear from the use of the adsorbent inorganic substance and polydiotatadecyl sheenan, there was a remarkable effect on promoting blood coagulation. ! & was confirmed, and the serum separation condition was also very good.

Example 1 In Example 1, the coating liquid was PSM, and the V polymer was Posidiotatadecyl PM, which had a V group in Etho (average molecular weight koji residue...) Tengame amorphous professional acid. Obtain the blood test volume in the same manner as in Example 11, except that diethanolamine was used in Step 19 as the weight (average particle size) and the ζy compound. Eh, the coating amount is tsxt・-'pr/
10Id, 14x the above polydiotatadecyl cypress
1O-4jlr/He(Id, Stearyl diethanolamine is 叡・×l・-' lpr/ 1・Od and circle.

Using the ζ・0j ILil test container, the IIL fluid identity, serum separation state, and serum yield were evaluated in the same manner as in Example 1. Natural amorphous silicic acid fine powder, I
II. When using polydiotadecyl V-roki and stearyl diethanolamine in warm conditions, a remarkable effect of promoting blood coagulation was confirmed, and the serum separation condition was also very good. .

Comparative example 1. Example of intention! and the same-4 method commercially available Polygum Piren blood machine eyebrow examination III (comparison l1l) and external IIE1/X1 internal value 1
4%, 41too% glass blood test spring 41 (comparison 11!) was prepared, and Example 1. ! Same article as KTh □
Subject KjllL liquid coagulation, serum separation state, and serum amount were evaluated experimentally.In Comparative Example 1, the coagulation time of the liquid was extremely long and tb, and the serum separation state was 6. ．． picture. or,
In Comparative Example 1, the reaction occurred in a relatively short period of time, but the adhesion to blood clots was suppressed.

Table 1゜

Claims (1)

[Scope of Claims] 1. Presence of heavy metal bodies and adsorbent inorganic substances in %y* on the inner layer surface, and 4 as good mold, harmful for blood cassette testing. ! A container for blood sac testing, characterized by containing an adsorbent inorganic substance, and an adsorbent compound.