JPH1164330A - Material and apparatus for separating gelled blood - Google Patents

Abstract

PROBLEM TO BE SOLVED: To reduce the viscosity of a gelled blood separating agent which is put in a blood collecting tube in advance by a small amount with the purpose of making the separation of blood serum and blood clot from each other easier, when the blood collected in the blood collecting tube is subjected to centrifugation so that the floating property and packing property of the separating agent may be improved and, at the same time, to improve the heat resistance of the separating agent and to inhibit the adsorption of a chemical into the blood. SOLUTION: A gel is prepared by blending 3-50 pts.wt. tackiness imparting agent (for example, an olefin or diolefin polymer), 1-30 pts.wt. inorganic filler for adjusting specific gravity (for example, talc), 1-10 pts.wt. thixotropy imparting agent (for example, an alkylammonium modified layered clay mineral), and 0.03-3 pts.wt. silane coupling agent with 100 pts.wt. hydrophobic liquid resin (for example, polybutene having a low specific gravity of 0.80-1.00.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a gel blood separating material used for separating blood, particularly blood for testing, and a blood separator containing the same in a container such as a test tube.

[0002]

2. Description of the Related Art The analysis of a blood sample for testing involves first separating the collected blood into a serum component and a clot component or a plasma component and a blood cell component by a difference in specific gravity, and only the component containing the analyte is analyzed. Is usually analyzed. This separation is usually performed by centrifugation, but may be performed by sedimentation.
For the purpose of facilitating this separation operation, a blood separation material having a specific gravity intermediate between the two components to be separated is used particularly in centrifugation.

When blood is centrifuged into the above two components in the presence of such a blood separating material, the blood separating material is located between the two components due to a difference in specific gravity and serves as a partition wall for separating the two components. Can easily be completely separated from the sedimentation component in the lower layer by the inclination. Therefore, only the necessary components of the blood sample can be completely removed and can be accurately tested, and the test accuracy is improved.

[0004] As a blood separation tube for performing the blood separation,
The blood collection tube (usually a test tube that can be sealed tightly, and a blood coagulant, anti-blood cell adhesion inhibitor, etc. is applied to the inner wall appropriately, because the labor of transferring the collected blood to another tube and blood loss at that time can be eliminated Is often used as is. In such a case, a small amount of blood separating material is filled in the blood collection tube.However, performing this operation by a nurse or the like at the time of examination complicates the blood collection operation. It is common to fill with a blood separation material.

[0005] The shape of the blood separating material filled in the blood collection tube is maintained so that the blood separation material does not move in the blood collection tube or flow out of the blood collection tube even if the blood collection tube is stored horizontally before or after shipment. A possible gel-like form is used, which gel is usually contained in the bottom of the blood collection tube. For a standard 10 ml blood collection tube, fill the bottom of the blood collection tube with 1-2 ml of gel, apply a vacuum and seal with a metal foil with a rubber stopper or rubber pad for puncture.

[0006] Such a gel-like blood separation material prevents movement and outflow of the blood separation material during storage, not only when the blood collection tube is filled, but also when it is filled into an independent blood separation tube. Of course.

[0007] Conventional gel-like blood separation materials are mainly composed of oily polymers such as silicone oil, chlorinated polybutene, polyisobutene, and olefin polymers, and silica, clay and the like for adjusting viscosity and specific gravity. Inorganic fine particles (thickening effect and thixotropic effect) and organic gelling agent (thickening effect) were added.

However, in this conventional blood separation material, inorganic fine particles are easily separated or run out and it is difficult to maintain the shape. As a result, a desired partition cannot be formed between the supernatant and the sedimented component. Easy to adsorb drugs contained in blood, resulting in inaccurate test results, incomplete separation of blood, blood cell components remaining on top of blood separation material, poor fluidity, blood separation in blood collection tube during centrifugation There were many difficulties, such as the difficulty in floating the material.

To solve these difficulties, Japanese Patent Laid-Open No.
No. 10945 discloses a blood separation method in which a tackifier (eg, a natural polyterpene resin such as rosin and a rosin derivative, or a hydrocarbon-based synthetic resin) is added to a material for forming a separation layer (the above-described polymer oil). Japanese Patent Application Laid-Open No. 5-80044 proposes a blood separation material further containing a lipophilic layered inorganic compound (mica, bentonite, smectite, etc.).

Japanese Patent Application Laid-Open No. 5-203640 proposes that a separation layer-forming material contain a wax.
No. 148175 proposes to use a styrene polymer which is liquid at room temperature as a material for forming a separation layer. JP-A-7-128329 discloses a molar ratio of propylene unit / α-olefin unit having 4 to 6 carbon atoms of 15/85 to 90 /.
Chlorinated copolymer at 10 (chlorine content 5-40% by weight)
It has been proposed to use as a material for forming a separation layer.

[0011]

However, none of the gel-like blood separation materials proposed in the above publications is completely satisfactory yet, and has the following problems.

For example, in all of these gel-like blood separation materials, the gel is made highly viscous with emphasis on shape maintenance.
Poor buoyancy of blood separation material during centrifugation, centrifugation takes longer than necessary, or separation material is
In some cases, it may not be able to reach the interface between the sedimentation component (eg, blood clot) and the sedimentation component (eg, blood clot), and may not be able to fulfill the purpose of the separation material. If the centrifugation time becomes longer than necessary,
May cause destruction and other adverse effects on blood cell components.

[0013] In addition, despite the high viscosity, the gel blood separating material described above has insufficient heat resistance and may not be able to reliably maintain its shape at high temperatures during storage. . In order to maintain the shape even when stored under high temperature conditions in summer, it is necessary to withstand a heat resistance test at, for example, about 55 ° C. for 24 hours.

Furthermore, since the gel has a high viscosity, it is difficult to fill the bottom of the blood collection tube, and the filling line is very expensive. Therefore, the filling cost is many times higher than the material cost of the blood separation material. That is the fact.

Another problem of the above gel-like blood separation material is that
Adsorption of drugs present in the blood is still occurring at a significant rate. Such chemicals are often the subject of analysis in blood tests. If the blood sample collected in the blood collection tube is centrifuged and tested immediately after blood collection, the effect on the test result is negligible because the adsorption is slight. However, many blood tests are performed not at the hospital where the blood was collected but at another laboratory, and it takes time from the blood collection to the test. During this time, at least a part of the chemical substances present in the blood in the blood collection tube is adsorbed by the gel-like blood separating material, and the adsorption rate is, for example, 20 to 50% or more. It is inevitable to become.

The present invention provides a blood separation method which has good gel floating and filling properties, has sufficiently high heat resistance, and has little adsorption of a drug in blood, and therefore can solve all of the above problems. It is an object to provide a material and a blood separator filled with the blood separating material.

[0017]

Means for Solving the Problems The present inventors have added a small amount of a coupling agent to a blood separation material to mix a liquid resin having a relatively small specific gravity as a main material and to mix various components as described above. As a result, the present inventors have found that a gel-like blood separation material capable of solving the above-mentioned problems can be obtained, and have reached the present invention.

Here, the present invention is characterized in that it comprises a hydrophobic liquid resin having a specific gravity of 0.80 or more and less than 1.00 as a main component, and contains a tackifier, an inorganic filler, a thixotropic agent, and a coupling agent. It is a gel-like blood separation material.

Here, the specific gravity means a ratio of the mass at 25 ° C. of the same volume of the material to the mass of water at 4 ° C. According to the present invention, there is also provided a blood separator comprising a container containing the above-mentioned gel-like blood separating material.

As a container for storing the blood separation material, a test tube (ie, a bottomed tube) such as a glass tube or a plastic tube is generally used. However, the container is not limited to a tube and can be centrifuged. However, other shapes such as plastic bags and bottles may be used. Of course, the test tube used for this container may be a blood collection tube for a blood test, which is the most typical container. In the following description, the container is referred to as a test tube.

[0021]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the blood separating material of the present invention will be specifically described for each component.

Hydrophobic Liquid Resin The hydrophobic liquid resin , which is the main material of the gel-like blood separation material of the present invention (hereinafter sometimes simply referred to as gel), is liquid at room temperature and is a non-polar high molecular organic material. It is a material that becomes a matrix of a gel constituting a separating material, similarly to what is referred to as an oily polymer or a polymer oil in the above-mentioned publication. If the liquid resin is hydrophilic, the gel will adsorb the components in the blood, resulting in an error due to the adsorption in the blood component test results. When a hydrophobic liquid resin is used as the main material, adsorption of components in blood does not occur, so that accurate test results can be obtained.

Since this material is used in contact with the blood for analysis, monomers and other low molecular substances (eg, catalysts)
Non-toxic materials with no elution, no blood cell destruction (eg, no charge), are suitable.

In the present invention, a material having a specific gravity of 0.80 or more and less than 1.00 and a specific gravity lower than that of water is used as the hydrophobic liquid resin in order to improve the floating property and the filling property of the gel-like blood separating material. When a liquid polymer material having a specific gravity of more than 1 is used as in the related art, the floating property is reduced, and it is difficult to fill the gel. The value of the specific gravity is preferably in the range of 0.80 to 0.95.

Hydrophobic liquid resins suitable for use in the present invention are room temperature liquid polyolefins, chlorinated polyolefins, and maleated polyolefins that meet the above-mentioned critical requirements. Among them, polybutene and maleated polybutene are preferred. Polybutene is
It is a liquid polymer obtained by polymerizing a portion mainly composed of isobutylene in a butane-butene fraction generated by naphtha decomposition, and is chemically a copolymer of isobutylene and a small amount of n-butene. Is called polybutene. Copolymers of isobutylene and polypropylene or other polyolefins, as well as chlorinated products and maleated products of polybutene and these copolymers, can also be suitably used in the same manner as polybutene, provided that the above specific gravity requirements are satisfied. .

Commercially available polybutene has an average molecular weight of about 300 to 4,000, and the higher the average molecular weight, the higher the viscosity. However, all are transparent liquid resins, and the specific gravity is within the above range. Polybutene used in the present invention,
Those having an average molecular weight in the range of 500 to 2,000 are preferred, and the specific gravity of such polybutene is 0.90 to 0.95.

Tackifier A tackifier is a material that, when incorporated into a polymeric material, can impart tackiness to the polymeric material or significantly increase the adhesiveness of the polymeric material. . Since the liquid polymer material having a relatively low viscosity, which is the main material of the blood separation material of the present invention, has substantially no tackiness, it can impart tackiness to the polymer material. Mix the agent. As a result, a sufficient adhesive force and cohesive force are imparted to the generated gel (that is, blood separating material), and the integrity of the gel is maintained even during centrifugation. In addition, the adhesive force enables the gel to be fixed and held at the filling position (usually at the bottom) in the test tube when the gel is stored in the test tube.

Typical examples of the tackifier include a solid or semi-solid at normal temperature, a softening temperature of 150 ° C. or less and an average molecular weight of about 2
It is a natural or synthetic thermoplastic resin of about 00 to 1500.
This resin is also required to be non-toxic, free from blood cell destruction, and free from elution of monomers and other low-molecular substances, like the liquid resin described above.

Examples of natural resins useful as a tackifier include rosin and various rosin derivatives (including rosin esterified products such as glycerin ester and pentaerythritol ester, and polymers and hydrogenated products of rosin and its esterified products). , A pinene polymer, a polyterpene-based resin and its hydrogenated product, terpene phenol and the like.

Examples of synthetic resins useful as tackifiers include olefin and diolefin polymers, cyclopentadiene resins, aromatic petroleum resins and their hydrogenated products, various aromatic resins (eg, phenolic resins) Alkylphenol-acetylene resin, styrene resin, xylene resin, coumarone indene resin, vinyl toluene-α-methylstyrene copolymer), alicyclic saturated hydrocarbon resin, and the like. Particularly suitable materials for the tackifier in the present invention are olefin and diolefin polymers.

The amount of the tackifier is selected in consideration of the compatibility with the hydrophobic liquid resin as the main material and the viscosity thereof, but usually 3 to 50 parts by weight per 100 parts by weight of the hydrophobic liquid resin. Parts, preferably 10 to 30 parts by weight. If the amount of the tackifier is too small, the adhesive strength and cohesive strength of the gel will be insufficient, and problems such as tearing of the gel and embracing of blood cells may occur during centrifugation. If the compounding amount of the tackifier is too large, the compatibility with the hydrophobic liquid resin is reduced, the separation of the tackifier is caused, or the viscosity of the gel is increased, and the floating property and the filling property are reduced. .

Inorganic Filler As described above, the blood separating material needs to have a specific gravity intermediate between serum or plasma, which is a supernatant component of blood, and a clot or blood cell component, which is a sediment. In that sense, the specific gravity of the blood separation material is preferably in the range of 1.038 to 1.052, preferably
1.044 ± 0.003, particularly preferably 1.044 ± 0.001.

In the present invention, since the hydrophobic liquid resin as the main material has a relatively low specific gravity, an inorganic filler is blended for the purpose of adjusting the specific gravity. As the inorganic filler, those which are stable and have no toxicity in blood are preferable, and those which can be used as carriers and excipients in the field of pharmaceuticals are most preferable. Examples of such inorganic fillers include precipitated barium sulfate, precipitated calcium carbonate, magnesium carbonate, calcium sulfate, talc, and the like. Inorganic fillers having a high thickening effect, such as silica, clay and diatomaceous earth, are less preferred in order to increase the viscosity of the gel, but may be blended together with the above-mentioned inorganic fillers in small amounts. .

The average particle size of the inorganic filler is not particularly limited, but is usually preferably about 0.05 to 10 μm. If the particle size is too small, mixing becomes difficult, and the thickening effect is large, and the gel becomes highly viscous. If the particle size is too large, the filler tends to separate in the gel.

The compounding amount of the inorganic filler is such that the specific gravity of the gel-like blood separating material has a desired value within the above range. Therefore, the required amount depends on the type and amount of the other components, but is usually 1 to 100 parts by weight of the hydrophobic liquid resin.
It is preferably in the range of 30 parts by weight, more preferably in the range of 5 to 20 parts by weight.

Thixotropic agent The thixotropic agent can be produced without substantial thickening.
It is added to the gel blood separation material of the present invention in order to enhance the shape retention at rest. By mixing the thixotropic agent, the gel at rest has sufficient shape retention, and the gel during storage is effectively prevented from moving from a predetermined filling position in the test tube or flowing out from the test tube. You. The thixotropy-imparting agent acts as a gelling agent when at rest, but loses its function as a gelling agent when an external force is applied.Therefore, it does not impair the mobility of the gel during centrifugation or filling in a test tube. There is almost no adverse effect on the floating and filling properties of the gel.

As the thixotropic agent, various conventionally known materials can be used, but a lipophilic layered inorganic material which easily swells by incorporating a large amount of a relatively low molecular weight organic polymer as a main material between layers. Is preferred. Such a lipophilic layered inorganic material is a material in which metal ions existing between layers of a layered clay mineral such as mica, bentonite, smectite and the like are replaced with lipophilic onium ions such as quaternary alkylammonium ions.

The mixing amount of the thixotropic agent is 1 to 10 parts by weight, preferably 2 to 3 parts by weight, per 100 parts by weight of the hydrophobic liquid resin.
It is preferred to be within the range of parts by weight. If the amount is too small, the outflow and flow of the gel due to the thixotropic property will not be sufficiently prevented. If the amount is too large, the thixotropic property is saturated, the viscosity of the gel increases, and uniform mixing becomes difficult.

A gel is formed only by blending the above-mentioned three additives of the tackifier, the inorganic filler, and the thixotropic agent into the hydrophobic liquid resin as the main material of the coupling agent, but the main material is formed. Is a liquid resin with a lower specific gravity than before and therefore a low molecular weight,
The gel retains poor shape, especially at high temperatures.

In the present invention, by adding a coupling agent in addition to these additives, even if a hydrophobic liquid resin having a low specific gravity, a low molecular weight and a low viscosity is used, a sufficient shape can be maintained in the gel. Succeeded in giving sex. This is because the coupling agent is a polyfunctional compound capable of reacting with the hydrophobic liquid resin, the tackifier, and the inorganic filler for adjusting the specific gravity in the gel. Beyond that, it can serve as a grafting or cross-linking agent to link these components.

As a result, since the components are bonded to each other via the coupling agent, the shape retention of the gel is remarkably enhanced. Therefore, even if the liquid resin has a low specific gravity and a low viscosity as described above. It is possible to obtain a gel having excellent shape retention. In addition, as a result of the gel being densified by this bonding, the adsorption of the drug in the blood by the gel is significantly reduced, and the drug is not adsorbed at all.

Furthermore, since the shape retention of the gel is significantly improved by the addition of the coupling agent, the compounding amount of the tackifier or the thixotropic agent can be reduced, and the viscosity of the gel can be made lower than before. Becomes possible. This lowering of the viscosity enhances the levitation and filling properties of the gel-like blood separation material, which facilitates centrifugation and greatly reduces the cost of filling the test tube with the blood separation material. Further, the densification of the gel also improves the heat resistance of the gel, and provides a gel-like blood separation material that can maintain the gel shape without flowing out of the gel from the test tube even when left at a high temperature.

As the coupling agent, any of the well-known silane coupling agents, titanate coupling agents, and aluminum coupling agents can be used. Of these, silane coupling agents are preferred. Silane-based coupling agent, vinyl group, epoxy group, amino group, methacryl group, reactive functional groups such as mercapto group,
It is a silane derivative containing an alkoxyl group (eg, methoxy group, ethoxy group) and / or a hydrolyzable group such as halogen (eg, chloro). As a whole or a part of the coupling agent, a silicone-based surface modifier (eg, polydimethylsiloxane) can also be used.

The amount of the coupling agent is preferably 0.03 to 3 parts by weight, more preferably 0.1 to 1.0 part by weight, per 100 parts by weight of the hydrophobic liquid resin. If the amount is too small, the above effect becomes insufficient, and if the amount is too large,
Excess coupling agent may separate.

The essential components of the gel-like blood separating material of the present invention are as described above, but optional components other than these may be added in a small amount for improving fluidity. Examples of such optional ingredients include waxes, plasticizers, blood coagulants, and the like. In addition, styrene, AB
An S resin, an AS resin, or a resin obtained by subjecting these resins to a hydrophobic treatment may be added.

The gel-like blood separating material of the present invention can be prepared by, for example, subjecting a hydrophobic liquid resin to an appropriate temperature (for example, 50 to 120).
While heating to (° C), the tackifier is first dissolved in this, the remaining components are added to the obtained resin solution, and stirring is continued for several hours until the resin solution is uniformly dispersed, or a roll (e.g.,
(3 rolls).

However, instead of directly adding the coupling agent to the hydrophobic liquid resin, an inorganic filler for adjusting the specific gravity may be added by surface-treating the coupling agent in advance. This surface treatment may be performed by a conventional method, and for example, treatment means such as immersion, spraying, and dropping using a solution of a coupling agent is possible. The inorganic filler treated in this manner may be heated to promote the coupling of the coupling agent to the filler.

The viscosity of the gel blood separation material of the present invention is 25 ° C.
The kinematic viscosity measured in the above is preferably 1,000 to 10,000 St, more preferably 3,000 to 6,000 St, and the specific gravity is 1.038 to 1.052, preferably 1.044 ± 0.003, particularly as described above.
1.044 ± 0.001.

The gel-like blood separating material of the present invention is suitably placed in a suitable container,
For example, filling a glass or plastic test tube produces a blood separator (tube). The filling amount of the gel-like blood separating material may be a sufficient amount as a partition wall between the supernatant and the sediment so as to separate them so that they do not mix during centrifugation. Usually, the filling amount is appropriately about 5 to 20% of the volume of the blood separator. The filling method is generally a pressure injection method. The blood separation material of the present invention is a gel having a lower viscosity than conventional products and has a thixotropic property, so that when an external force is applied by stirring or the like, the fluidity is remarkably improved, so that the material is not heated to a very high temperature. Also, the filling operation becomes much easier, and the filling cost can be greatly reduced. The filling temperature is suitably from 25 to 70 ° C.

The inner wall of the filling container may be coated with an appropriate agent conventionally used for blood collection tubes, such as a blood coagulant or an anti-blood cell adhesion agent. This is the case when used as a blood collection tube.

As described above, the container for filling the gel-like blood separating material is not limited to a test tube, and other containers that can be used for separating blood, such as bags and bottles, can also be used. After the container is filled with the gel-like blood separating material, the inside of the container is usually evacuated to a vacuum, and the container is sealed (sealed) by an appropriate means to produce a blood separator (eg, a blood collection tube). In the case of a test tube, it may be sealed with a rubber stopper or metal foil as described above.
It is preferable that a portion for injecting blood (for example, a rubber pad portion for puncturing) is formed in the metal foil. Although the sealed container is stored until use, the separating material of the present invention is excellent in heat resistance, so that the gel shape at the time of filling can be maintained even if the temperature becomes high during storage.

[0052]

【Example】

(Example 1) 100 parts by weight of polybutene (Idemitsu Polybutene 15R) having a specific gravity of 0.87, an average molecular weight of 570 and a kinematic viscosity of 720 cSt at 40 ° C, and an alicyclic saturated hydrocarbon resin (tackifier, Arakawa Chemical Co., Ltd.) (Alcon P-90) and 40 parts by weight were heated and stirred at 120 ° C. for 1 hour. Talc surface-treated with 0.2 parts by weight of γ-mercaptopropyltrimethoxysilane (silane-based coupling agent, KBM803 manufactured by Shin-Etsu Chemical Co., Ltd.)
(Inorganic filler) 50 parts by weight and 2 parts by weight of an alkylammonium-modified natural smectite (thixotropy imparting agent, SD-1 manufactured by NL Chemicals Co., Ltd.) are added and kneaded with a three-roll mill, specific gravity 1.040 at 25 ° C. Kinematic viscosity measured at 2,50
A gel blood separation material A of 0 St was obtained.

1.5 ml of the blood separating material was filled into the bottom of a 10 ml glass test tube, and a hollow having a depth of about 5 mm was formed at the center of the surface of the filled gel layer by air blowing.
And kept at 55 ° C for 24 hours to examine the heat resistance.
There was no change in the shape of the depression, the original gel shape was retained, and the heat resistance was good.

Gel blood separation materials B and C were obtained in the same manner as above, except that the amounts of the components were changed to the amounts shown in Table 1 (all parts by weight). The specific gravity of these gel blood separation materials was 1.043 for B and 1.045 for C, and the kinematic viscosities measured at 25 ° C. were 4,000 St for B and 4,500 St for C. Test tubes were filled with these blood separation materials in the same manner as above, and the heat resistance was evaluated.

[0055]

TABLE 1 Ingredient separation material A separation material B separator material C polybutene 15R (liquid resin) 100 100 100 Arkon P-90 (tackifier) 40 40 50 KBM-803 (coupling agent) 0.2 0.5 1.0 Talc ( Inorganic filler) 50 10 20 SD-1 (Thixotropic agent) 2 5 10 Specific gravity 1.040 1.043 1.045 Kinematic viscosity (25 ° C, St) 2,500 4,000 4,500 Heat resistance (55 ° C, 24t time) Good Good Good Gel of the present invention The blood-like blood separation material, despite its low viscosity, has good gel shape retention and heat resistance, and does not flow or flow out of test tubes even when left at high temperatures during storage. It has been found.

When 2 cc of a whole blood sample was placed in each of the above test tubes filled with blood separating materials A to C at the bottom, and left for 1 day, the mixture was centrifuged at 1200 G for 5 minutes. It was possible to separate a serum component which was located between the mochi component and was not mixed with blood cells from a blood clot component due to the inclination.

Comparative Example 1 A blood separation material containing no coupling agent was prepared by repeating Example 1 except that talc (an inorganic filler) was used without surface treatment with a silane coupling agent KBM-803. A ′ to C ′ were obtained. The compositions of the obtained blood separation materials A ′ to C ′ are the same as those of the blood separation materials A to C of Example 1 except that the amount of the coupling agent is 0, and the specific gravity and the kinematic viscosity are also different. Same as AC.

When the heat resistance of the blood separation materials A ′ to C ′ containing no coupling agent was tested in the same manner as in Example 1, the blood separation materials were separated at a temperature of 55 ° C. for 24 hours, and then flowed out. It became impossible to perform the separation function.

The drug-adsorbing properties of the gel-like blood separation materials A to C (containing a coupling agent) and A 'to C' (without a coupling agent) were measured as follows.
(Local anesthetic) and haloperidol (antipsychotic) are shown in Table 2 as drug residual rates. The larger the value of the residual ratio, the lower the drug adsorbability of the blood separation material.

Drug Adsorption Test An ethanol solution of lidocaine is diluted 40 to 60-fold with human serum to prepare a drug-containing serum sample having a concentration of 5 μg / ml.
Similarly, haloperidol in ethanol is also used in human serum.
Prepare a drug-containing serum sample at a concentration of 15 ng / ml by diluting 40- to 60-fold. Each 1 ml of each drug-containing serum sample prepared was
Inject 1 ml of the gel to be tested and dispense into each of a blood collection tube having a flat upper surface of the gel and an empty blood collection tube into which the gel has not been injected. Thereafter, the residual amount of the drug in the serum sample of each test tube is measured, and the residual ratio of the drug is determined as (remaining amount / remaining amount) × 100 (%).

[0061]

[Table 2]

As can be seen from Table 2, the gel blood separating material of the present invention is remarkably superior to the conventional product in adsorbing the drug, and hardly or not adsorbs the drug even when left in contact with blood for a long time. In addition, the accuracy of the test when testing a left blood sample is significantly improved, which is useful for accurate diagnosis and treatment.

[0063]

As described in detail above, the gel-like blood separation material of the present invention has a lower viscosity than conventional products, so it has excellent floating and filling properties during centrifugation, and the centrifugation time cannot be shortened. Since there is no need to lengthen the length, adverse effects on blood due to centrifugation can be minimized, and a blood separator can be manufactured at low cost.

Despite being a low-viscosity gel-like substance, the heat resistance is good due to the action of the coupling agent, the shape retention during storage is excellent, and even if the storage conditions are high, the inside of the container is not affected. Therefore, the blood separating material does not flow or further out of the container.

Furthermore, due to the action of the coupling agent, the gel-type blood separating material of the present invention has very little adsorption of the drug contained in the blood and which can be a test target, and does not adsorb any drug at all. It has been greatly improved in terms of gender. Therefore, a blood test can be accurately performed.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Daisuke Shibuta 1-297 Kitabukurocho, Omiya City, Saitama Prefecture Mitsubishi Materials Research Institute (72) Inventor Hideomi Katano 3237 Togasaki, Misato City, Saitama Prefecture Katano Dyed Leather Co., Ltd. Company Saitama Chemical Factory

Claims (4)

[Claims] 1. A gel-like composition comprising a hydrophobic liquid resin having a specific gravity of 0.80 or more and less than 1.00 as a main component, and containing a tackifier, an inorganic filler, a thixotropic agent, and a coupling agent. Blood separation material. 2. The gel blood separation material according to claim 1, wherein the hydrophobic liquid resin is polybutene. 3 to 50 parts by weight of a tackifier, 1 to 30 parts by weight of an inorganic filler, 1 to 10 parts by weight of a thixotropic agent, and a coupling agent with respect to 100 parts by weight of a hydrophobic liquid resin.
The gel-like blood separating material according to claim 1 or 2, comprising 0.03 to 3 parts by weight. 4. A blood separator comprising a container containing the gel blood separating material according to claim 1.