JPS5835443A - Photometer - Google Patents

Abstract

PURPOSE:To enable to perform a detection of an intermediate point of filteration in conformity with the motion of a syringe, by installing a cuvette for optical photometery conforming to a photometer making a measurement of fluorescence and scattered light after verifying that suspension can be filtered by a porous filter. CONSTITUTION:In the case of suction filteration, a tube vertical moving mechanism 8 provided with a flow path system tube 7 is first lowered, and the tube 7 is inserted into a cuvette 1 for suspension photometry. Then, a switch valve 12 closes off the flow path, and in a spindle vertical moving mechanism 11 positioned at a lower dead point, a position detector 13 detects the state by means of a detecting panel 15. If a suction filteration starts, a piston 10 in a syringe 9 is lifted in a rise direction by the piston vertical moving mechanism, and in which case, the detecting panel 15 is detached from the position detector 13 to detect starting of filteration. Suspension 2 in the cuvette for suspension photometry is introduced to a flow path tube, particles in the suspension 2 are filtered by a porous filter 3, and filterate 4 is stored in the syringe.

Description

Detailed Description of the Invention The present invention relates to a photometer that requires filtration, and is particularly suitable for performing automatic filtration and measuring optical intensity, and is capable of automatically detecting the start and completion of filtration. Regarding.

FIG. 1 shows an example of the prior art.

The channel system tube 10 is inserted into the suspension photometry cuvette 1, and vacuum suction is applied in the direction shown by the arrow in the figure. The suspension 2 in the cuvette by suction is filtered by the porous filter 3, and only the filtrate 4 is collected into the filtrate photometric cuvette 8.

The rubber plug 9 prevents air leakage during suction filtration.

The above-mentioned conventional technology has the following drawbacks.

(1) A rubber stopper must be placed in the filtrate photometric cuvette each time filtration is performed. Therefore, the operation is extremely complicated and the cuvette may be contaminated. Furthermore, when setting a rubber stopper in a cuvette, the cuvette is often damaged.

(11) When filtering the reaction product, it is generally assumed that the reaction is proceeding until the intermediate time of filtration (the midpoint between the start of filtration and the completion of filtration). Therefore, detection of intermediate time is essential. However, in the prior art, it is extremely difficult to detect intermediate times of filtration.

In the GiD conventional technology, only suction filtration is possible, and filtration by pressurization is not possible.

The present invention is intended to eliminate the drawbacks of the prior art described above and to improve the operability of filtration measurement.

The purpose of the present invention is to automatically filter a suspension liquid, collect a filtrate, detect a filtration midpoint necessary for filtration of suspended reactants, and perform optical measurements. The object of the present invention is to provide a suitable photometer.

The present invention confirms that a suspension can be filtered by a porous filter by vacuum or pressure, and the arrangement of the filter, a vacuum (suction) or pressure syringe, and a cuvette for optical photometry can be used for fluorescence, It is made to match a photometer that measures scattered light, transmitted light, etc., automatically performs optical measurement and filtration, and detects the intermediate point of filtration in conjunction with the operation of the syringe. be.

An embodiment of the invention is shown in FIG.

When performing suction filtration, the tube up/down mechanism 8 to which the mass channel system tube 7 is attached is lowered, and the tube is inserted into the suspension photometry cuvette 1. At this time, switching valve 1
2 closes the flow path. In addition, the piston up and down mechanism 11 is
It is at the bottom dead center, and the position detector A13 on the detection plate 15 detects the state.

When suction filtration starts, the piston 10 in the syringe 9 is pulled up in the upward direction by the piston up and down mechanism. At this time, the detection plate moves away from the position detector (ON→0
FF), it is detected that filtration has started.

The V suspension 2 in the suspension photometry cuvette is led to a channel system tube, and particles in the suspension are filtered out by a porous filter 3. The filtrate 4 is stored in the rim. When the piston up and down mechanism moves up and the detection plate reaches the position detector B14, the piston stops at the top dead center and suction filtration also stops.

The point at which the piston stops is the point at which filtration is complete.

The start point and end point of filtration are detected by the signals from position detector A (ON→OFF') and position detector B (OFF→ON). The midpoint of time is found.

Filtration amount starting point: T.

2T when filtration is completed.

Filtration midpoint time: (T, +T2)/2 When filtration is completed, the switching valve 12 opens, the piston up and down mechanism descends,
The filtrate is collected into a filtrate photometric cuvette 5. At this time, the porous filter is clogged with suspended particles, and the resistance of the flow path increases significantly, so that the filtrate does not flow into the suspension photometry cuvette.

In addition, the flow path tube through which the filtrate flows through the switching valve is
The inner diameter should be large enough to allow the filtrate to be smoothly injected into the filtrate photometry cuvette.

In the operation described above, the suspension in the suspension photometric cuvette is filtered by a porous filter, and the filtrate is collected in the filtrate photometric cuvette.

At the same time, the start and end points of filtration are detected by position detection of the piston up-and-down mechanism, and the filtration intermediate point time is detected.

An example of a photometer for fluorescence measurement according to the invention is shown in FIG.

The excitation light separated by the excitation side spectrometer 16 enters a photometric cuvette containing a suspension of biologically related substances.

Fluorescent light emitted from the sample in the cuvette is spectrally separated by a fluorescent side spectrometer 17 and then reaches a photometric detector 18. After measuring the fluorescence intensity of the sample in the cuvette for a certain period of time, filtration is performed by the method described above. The filtrate photometry cuvette from which the filtrate has been collected is set at the photometry position by rotating the rotary cuvette holder 1800 revolutions. After that, the fluorescence intensity of the filtrate is measured.

According to one embodiment of the present invention, the following effects are produced.

(1) Filtration can be performed with the photometric cuvette installed in the device.

Therefore, in order to perform filtration, there is no need to take out the cuvette to the outside, and since the filtrate is automatically collected into the cuvette for filtrate measurement, there is no need to transfer the filtrate.

Operation is simplified.

(i+) 9 It becomes possible to measure the optical intensity originating from the suspension particles. At the same time, it becomes possible to perform measurements with interference components caused by turbidity particles removed, thereby improving photometry accuracy.

There is no need to attach a filtration application member to the cuvette using a GiD rubber stopper or the like. When installing the cuvette, it is no longer possible to stain or damage the cuvette.

(It is possible to detect the starting point and end point of ψ filtration, and it is also possible to easily determine the intermediate point of filtration.

Therefore, in a measurement involving a reaction of V turbid particles, it is possible to accurately detect the time at an indispensable intermediate point in filtration (assuming that the reaction has proceeded up to the intermediate point in time).

(Since ψfiltration can be performed without taking the photometric cuvette out of the device, the temperature of the cuvette can be controlled strictly and accurately.

In the examples of the present invention, pressure filtration becomes possible when filtration is performed under the following conditions. Therefore, in the present invention, both suction filtration and pressure filtration are possible, and depending on the purpose, suction or pressure filtration is possible.

Open the switching valve and insert the tube opposite the tube equipped with the porous filter into the suspension photometry cuvette. After that, move the syringe in the suction direction. After closing the switching valve, move the syringe in the discharge direction. ! [
Particles in the liquid are filtered by a porous filter, and the filtrate is discharged into a filtrate photometric cuvette. Pressure filtration is effective when the particles are solid.

[Brief explanation of the drawing]

FIG. 1 shows a filtration system according to the prior art. Figure 2 shows
An example of a filtration system according to the present invention is shown. FIG. 3 shows an example of implementing the filtration method according to the invention in a fluorometer.

Claims (1)

[Claims] 1. In a photometer that measures the optical intensity of a suspension and a filtrate obtained by filtering particles in the suspension, a cuvette for suspension photometry, a cuvette for filtrate photometry, and a 1. A photometer comprising: a tube forming a flow path system; a porous filter for filtration; a syringe for inhaling and discharging a solution; and a flow path system switching valve.