JPS5830651A - Analyzer for organism component - Google Patents

Abstract

PURPOSE:To normally keep a buffer solution at a constant temperature, to reduce a measuring solution to a trace amount, and to provide a device which is miniaturized and excellent in precision, by mounting a temperature controller outside a buffer solution vessel and using a conical measuring cell. CONSTITUTION:A cycling pump 3 removes a buffer solution through a pipe positioned in the buffer liquid 2 in a buffer liquid vessel 1, and the buffer liquid is supplied to a pipe 6 wound around a heating drum 4 to bring it into a constant temperature. The buffer liquid from a first cylinder 8 is fed to a pipe 7 wound around a heating drum through the pipe, and after it is brought to a constant temperature, it is supplied to a reaction chamber 28 in a measuring cell 25. Liquid to be measured, which is prepared such that a reagent, placed in a reagent vessel 23, and a sample, placed in a sample vessel 20, are mixed, is supplied through a nozzle 18, and is mixed with the buffer liquid and is analyzed by an electrode 26 for analyzing an organism component. The measuring cell 25 used with a subject analyzer has a side formed in a regular octagonal cone, a reaction chamber is miniaturized in order to install an electrode to the slope of the side, and a measuring solution can be reduced to a trace amount.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a biological component analyzer for analyzing components such as total cholesterol, neutral lipids, phospholipids, and gluten.

Conventional biological component analyzers can be broadly divided into three problems. Seventh, how to stabilize the temperature of the buffer solution, send it to the reaction chamber for measurement, and how to keep the temperature of the buffer solution etc. constant in the reaction chamber. The first is,
The liquid to be measured, which is a combination of a biological sample (hereinafter referred to as the sample) and a reagent, is kept stably at the measurement temperature to allow the sample and reagent to sufficiently react, and then sent to the reaction chamber for measurement. be. The third method is to minimize the amount of buffer solution and sample solution injected into the reaction chamber for measurement, and immediately after injection, the buffer solution and sample solution are brought to a temperature close to the measurement temperature, and are immediately heated to the measurement temperature. Therefore, it is important to have a fast response speed while minimizing measurement errors due to errors in measured temperature. Regarding the three problems mentioned above, the disadvantages of the conventional device will be described in detail using the conventional device as an example.

First, conventional devices that keep the buffer solution at a constant temperature have a temperature detector, an immersion heater, a stirring shaft, etc. inside the buffer container. Therefore, when replacing the buffer solution in the buffer container with a new buffer solution, the temperature sensor, immersion heater, stirring shaft, etc. had to be removed, which was very time-consuming and troublesome. . Also, when sending the buffer solution in the buffer solution container to a predetermined device, if the tube from the buffer solution is long to reach the reaction chamber for measurement, the tube will take away the heat from the buffer solution, and therefore the measurement will be delayed. There was a drawback that the temperature of the buffer solution that reached the reaction chamber for the measurement was lower than the measurement temperature. Furthermore, even when the buffer solution in the buffer solution container runs out, the temperature sensor, immersion heater, and control device continue to operate even if the buffer solution container is filled with buffer vapor because the buffer solution container is sealed. It is extremely dangerous as it will become hot and dry.

The second problem is to keep the temperature of the liquid to be measured constant to ensure a sufficient reaction.In recent years, turntables used in biological component analyzers6 simply hold a sample container containing a sample. and move it to the required location. Even if the sample and reagent were placed in a test tube without being placed in the measurement cell, the large volume of the test tube would require a large amount of sample and reagent, which would be wasteful, and it would take a lot of equipment to maintain the temperature at a constant temperature. It turned out to be quite a big deal.

The third problem is the response speed.The measurement cell of conventional biological component analyzers has a cylindrical shape with the same inner diameter from the injection port for the liquid to be measured to the reaction chamber, so it reacts with the electrode for biological component analysis. It was not possible to minimize the amount of the mixed solution of the buffer solution and the sample solution. In addition, since the biological component analysis electrode is installed horizontally, if the air bubbles contained within are at the tip, they will be difficult to move and may not be detected sufficiently, so the electrode for biological component analysis must be made thicker. There were drawbacks. When the electrode for biological component analysis is made thicker, the reaction chamber must be made larger, and when the reaction chamber is made larger, the amount of the mixed solution of the liquid to be measured and the buffer solution must be increased.

The purpose of the present invention is to provide a biological component analyzer that eliminates the above-mentioned drawbacks. In response to miniaturization of the amount of water, we provide a compact device with good responsiveness and high measurement accuracy. Its features include a temperature control device that keeps the buffer solution in the buffer solution container at a constant temperature; a turntable for holding the sample; a mixing and transporting means for mixing and transporting the sample and reagent; and a liquid to be measured mixed by the mixing and transporting means. The buffer solution is injected into the reaction chamber in the measurement cell through the injection passage using a ring ring and mixed with the buffer solution, and the mixed solution is used for biocomponent analysis using a biocomponent analysis electrode. It has a temperature control device that heats the liquid to a constant temperature using a circulation pump outside the liquid container, and a reaction container disc that is removably attached to the inside of the container that holds the sample and has a large number of reaction containers. A turntable with a reaction container equipped with a heater for heating the reaction container of the disc to a constant temperature and a film with good lubricity on the top of the heater, and a reaction chamber in the measurement cell with at least a part of the side surface making an acute angle with the lower surface. and an injection passage having a narrow shape on the side of the reaction chamber to prevent the solution in the injection passage above the narrow injection passage on the reaction chamber side from being agitated due to the reaction in the reaction chamber. The main feature is that it is equipped with a measurement cell.

An embodiment of the present invention will be described below with reference to the drawings. Fig. 1 is a schematic diagram showing the entire biological component analyzer of the present invention, and Fig. 2 is a diagram summarizing the schematic diagram of Fig. 1 in a block diagram and adding an electrical system. 4 is a cross-sectional view of the temperature control device excluding the circulation pump used in the biocomponent analyzer of the present invention. FIG. 4 is a turntable with a reaction container used in the biocomponent analyzer of the present invention, and FIG. , FIG. 6 is a top view of a measurement cell used in the bioanalyzer of the present invention, and FIG.
FIG. 3 is a cross-sectional view of the measurement cell shown in the figure. Below, the configuration will be explained according to the drawings. In Figure 1, a buffer solution container 1 contains a buffer solution 2 at a constant temperature (for example, 37°C in Japan according to the measured temperature of a living body). A tube containing a buffer solution discharged from inside 2 by a circulation pump 3 is a tube 6 spirally wound around a heating drum 4, and its starting point is X and its ending point is X'. The heating drum 4 is equipped with a heater and a temperature sensor, which are connected to the first temperature controller 5 at the end point X of the tube 6 spirally wound around the heating drum 4. ' is again introduced along the wall of the buffer solution container 1 via the circulation pump 3. The circulation pump 3, the heating drum 4, the first temperature controller 51, and the tube spirally wound around the heating drum are collectively referred to as a temperature control device. The light from the light source 35 is made into parallel light by a lens, passes through a glass tube 36', and passes through a light receiving element 36.
to go into. An amplifier is connected to the light receiving element, and then a signal is input to a microcomputer through an analog-to-digital transformer. The microcomputer controls the power supply switching circuit based on this input signal (these circuits are not shown). In addition, another tube is contained in the buffer solution 2, and this tube is connected to the opening of the first switching valve 9, a.
It is connected to the. The first cylinder 8 is connected to the opening, b, of the first switching valve 9, and the opening, a', of the second switching valve 10 is connected to the opening, C, through a pipe.

The opening b' of the second switching valve 10 is connected to a second cylinder 12, and the second cylinder 12 sucks in the sample and reagent from the first nozzle body 15, mixes the sample and the reagent, and transfers the sample and reagent to the liquid to be measured. The opening of the second switching valve 10, a', which is connected via a pipe to the nozzle 17 for the liquid to be measured, which is discharged as a liquid, is further connected to the opening of the third switching valve 11, Hatake', via a pipe. , the mouth of the third switching valve, C, is connected to the third cylinder 13 via a pipe, and the third cylinder 1
3 is a reaction container 21 of the turntable 19 with a reaction container.
The second nozzle 1 conveys the liquid to be measured inside to the measurement cell 25.
A pipe 6 spirally wound around the heating drum 4 is connected to the opening of the zero switching valve 11, b", which is connected to the heating drum 4 through a pipe.
Above the tube is a spirally wound tube 7 whose starting point is Y and whose ending point is Y'. The tube exiting the end point Y' of the tube 7 spirally wound around the heating drum 4 is the measuring cell 2.
It is wound around a circumferential groove 29 in 5. Measuring cell 25
The tube wound inside the housing 29 is the reaction chamber 28.
It is connected to the. The mouth of the first switching valve 9, b, is a fixed end and can be switched to connect b and a or b and C. The mouth of the second switching valve 10, a', is a fixed end and is switched to connect a' and b' or a' and a'. The opening of the third switching valve 11, a", is a fixed end, and a" and b" are switched to connect aII and cll. The silicone oil nozzle 16 of the first nozzle 15 has a fourth The first nozzle 15, to which the cylinder 14 is connected via a pipe, can move vertically and horizontally as shown by the arrows.
), (31, (41*(5) position is the first nozzle 1
5 shows the stop position.

The second nozzle 18 can also move up and down and left and right as shown by the arrows, and the position of the force indicates the stop position of the second nozzle (f6 in the drawing). A washing tank 24 is arranged directly below the nozzle 17 for liquid to be measured at position (2), and a container 22 in which liquid to be measured is placed directly below the nozzle 16 for liquid oil at position (2) and the nozzle 17 for liquid to be measured at position (2). A container 23 containing a reagent is arranged.A sample container containing a sample is arranged directly below the nozzle 17 for the liquid to be measured at the position (3). A reaction container 21 is arranged directly below the measurement liquid nozzle 17, and the reaction container 21 is located at the position (5) (the first nozzle 15
A reaction vessel is arranged directly below the silicone oil nozzle 16 (not shown). The sample container 20 and the reaction container 21 are placed directly below the nozzle by rotating the turntable 19 with the reaction container. Measuring cell 2
5 has an injection passage 27 for injecting the liquid to be measured into the reaction chamber 28, the measuring liquid injected into the reaction chamber 28, and the tip of a biological component analysis electrode 26 for measuring 1. The reaction chamber 28 is equipped with a heater 30. It is heated to a constant temperature by a temperature detector (not shown) and a second temperature controller 34i. The waste liquid outlet of the reaction chamber 28 is connected to a waste liquid tank 32 via a fourth switching valve 37 via a pipe, and the other side of the waste liquid tank 32 is connected to a vacuum pump 33 by a pipe. The discharge port of the washing tank is also connected to the waste liquid tank via a fourth switching valve 37. the mouth of the fourth switching valve 37;
aII is a fixed end, I/ and bl/ or '〃. 〃It can be switched to connect with I.

Immediately below the second nozzle 18 at position (6), there is a reaction vessel 2 containing the liquid to be measured in which the sample and reagent have sufficiently reacted.
1 is arranged, and the injection passage 27 of the measuring cell 25 is arranged directly below the second nozzle 18 that has come to the force position.The first nozzle and the second nozzle are collectively called a mixing conveyance means .

Figure 2 shows the first and second biological component analyzers depicted in the figure.
Where the numbers in the figures are the same, the parts in the drawings correspond to each other. Power supply lI3 is input to microcomputer IIq. Micro Conview Tag q incorporates all necessary interfaces.

Inputs from the key buttons are input to the microcomputer via the keyboard I12. The microcomputer causes the device within the dotted line to perform a predetermined operation according to its software. The valve lI is a cylinder lI containing the first to third switching valves, 101// of FIG.
S is the first cylinder in Figure 1, /2, /3
, /ll. - is the first in cylinder llj
The nozzle /I (including the silicone oil nozzle /B and the measured liquid nozzle /7) is connected to the second nozzle /I. The buffer solution 2 passes through the circulation pump 3, is heated to a constant temperature by the heating drum t, and returns. The returned buffer passes through the valve II and the cylinder IS, and then returns to the measuring housing lI (injection passage 27), which is heated again by the heating drum l.
and two reaction chambers). The measuring housing has # for washing liquid! The liquid is drained into a waste liquid tank 33 by a vacuum pump 37 via a switching valve 37.

The qth switching valve 37 is also connected to the washing tank 21I. In the measurement housing 'lf, the tips of the biological component analysis electrodes, etc., are in contact with the solution in the measurement housing tIg. Furthermore, there is a signal processing circuit +9 in the biological component analysis electrode 26 and the like, and the output of the signal processing circuit is inputted to the microcomputer II<2. The output of the microcomputer <21I is sent to the display device 39 or the splinter.
The signal is output to the recorder 11O' via Ilo and the output terminal 1I/, and is displayed or recorded. 1st nozzle/
The stopping positions of 3 are (1), +21, [31,141, +5
+,! : ! ; There are m locations. When the direction of the arrows of the silicone oil nozzle/6 and the liquid to be measured nozzle/7 is upward, it means suction into the nozzle, and when it is downward, it means discharge. If no arrow is shown, it means that the nozzle does not perform suction or discharge at that position. The same thing can be said about the second nozzle position (6+, f7). Immediately below the nozzle are a washing tank 21I, a container containing silicone oil, a container 4t7 containing reagents, a turntable with a reaction container, and a sample container 20 containing a sample. Each of the reaction vessels, 2/, and the measurement housing 48 of the measurement cell 25 is arranged corresponding to the position of each nozzle.

Although the position of 5) is not shown, the position of (5) is when the silicone oil nozzle 16 comes to the position of the liquid to be measured nozzle 17 at position (4).

In FIG. 3, the heat transfer body 51 has a cylindrical shape. The cylindrical end face is a face perpendicular to the plane of the paper, and the heater 45 and temperature sensor 53 enter the heat transfer body 51 from the - face. These heat transfer body 51, heater 45, and temperature detector 53 are collectively called heating drum 4.

A circulation pump 3 shown in FIG.
The tube from 1 to 3 is wound in a spiral shape with a starting point X and an ending point X' in contact with the heating drum 4. On the outside, there is a first
The pipe from the third switching valve 11 shown in the figure is spirally wound outward from a starting point Y to an ending point Y' as shown in FIG.

Insulating material 50 is added to the outside of the spirally wound pipe as necessary.
It is covered with The heater 52 and the temperature detector 53 are the first
The heating drum 4 is connected to a temperature controller 5 to control the temperature of the heating drum 4. FIG. 4 is a more detailed drawing of the turntable 19 with reaction vessels shown in FIG. The first fixed disk 10 is arranged in the following manner.
1 is fixed to the turnchill pull body 120. 1st
A reaction container disk 102 is placed on the turntable main body 120 inside the fixed disk 101 and is detachable. A large number of reaction containers 21 are provided in an annular shape on the reaction container disk 102 so as to correspond to the large number of holes in the fixed disk 101 . Furthermore, there are three holes 10 inside ζ
5 is provided, and the turntable body 12 is connected to this hole.
The center portion 128 of 0 is bored. Therefore, by inserting your fingers into the three holes 51, the turntable body 1
Q. In this figure, the reaction vessels 21 are arranged in a ring in a row, but there is a first fixed disk in the center, which may be arranged in multiple rows. A second fixed disk 103 is provided apart from the bottom. The second fixed disk 1034 has a large number of holes 107 with a slightly larger diameter formed in an annular shape corresponding to the large number of sample container holding holes 106 for holding sample containers in the first fixed disk. That's how it is.

On the outside of the large number of holes, a large number of small holes 108 for position detection are arranged in an annular shape corresponding to the large number of large diameter holes '107. Furthermore, there is a small hole for detecting its position.

A hole 109 for initial position detection is provided outside of the hole 108 . This second fixed disk 103 is fixed to the turntable main body 120. A first detector 111 for detecting a large number of small holes 8 for position detection is provided, and an L-shaped jig No. 113 is fixed to a first fixing base 115. A second detector 110 for detecting the initial position detection hole 109 is also provided, and this is also attached to a second fixing base 114 with an L-shaped jig 112. In actual use, a large number of sample containers are used in the sample container holding hole 106, but they are omitted in FIG. 4.

FIG. 5 is a cross-sectional view of FIG. 4, showing a first fixed disk 101, a reaction container disk 102, a second fixed disk 103, and a reaction container 21.
．． Three holes 105, a sample container holder 106, a large number of large and diameter holes 107 provided in the second fixed disk, a small hole 108 for position detection, a second detector 110, and a first detector 11.
1. L-shaped jig 112.11'3, second fixing table 11
4. The relationship between the first fixing base 115 and the turntable main body 120 is as explained in FIG. are held in the 0 first and second
The detectors 111 and 110# are arranged with a second fixed disk in between, with a light emitter on one side and a light receiving element on the other side. There is a mounting table 1°26 fixed so as to face the turntable body 110. Between the mounting table 126 and the rotating surface of the turntable 120, there is a lubricating and heat-resistant film//particle such as a Teflon film 118 and a heater. 116 is attached to the mounting table side. Further, ball bearings 121 and 122 are installed between the shaft of the turntable main body 120 and the mounting base. A lead wire 117 is attached to the heater 116, which is connected to a power source to warm the turntable body, and a temperature detector 127 detects and adjusts the temperature to keep the sample and reagent in the reaction container 21 at a constant temperature. Warm to. A gear 12'3 is attached to the end of the shaft of the turntable body 120, and meshes with a gear 124 attached to the rotating shaft of the pulse motor 125.゛The first fixed table and the second fixed table 114, 115 are the mounting table 1.
The measuring cell 25 of FIG. 1 is shown in more detail in FIGS. 6 and 7.
To explain, the first block 207 has the shape of a regular octagonal prism with a flat top and a pyramid-shaped side. In order to fit the third block 208, a stepped through hole is bored in the upper part, which is smaller than the lower part, and in the lower part, the third block 208 is fitted.
9 is fitted into a circumferential groove 29, and a groove 212 through which a buffer tube 211 passes is provided. Four through holes 214 for attaching the tubes 210, 216, and 218 are formed at equal intervals, and one through hole 215 is formed through which the pipe 211 passes. The second block 20B has a stepped cylindrical outer shape, and has a nozzle-shaped injection passage 27 for injecting the sample in the upper part, and a reaction chamber 28 in the lower part with the fourth block 217. A cone-shaped cavity for the sample in the reaction chamber 28 is connected to the mounting hole 214 of the biological component analysis electrode 26 provided on the cone-shaped side surface of the first block 207. Through-holes 220 are formed in the tips 219 of the electrodes 26 and the biological component analysis electrodes 26, for example, for causing an immobilized enzyme reaction in an immobilized enzyme membrane. The third block 209 fits into a circumferential groove 29 provided on the underside of the first block 207 to form a housing in which a buffer tube 211 is housed. 30 is a buffer tube 21
1 buffer solution and the mixed solution of the test liquid and buffer solution in the reaction chamber 28 are transferred to the first block 207, the second block 208,
A heater is used to maintain the temperature at 37° C. via the third block 209, and sensors (not shown) for controlling this temperature are provided in the second block 208 and the third block 209. 222 is a heat insulating block that prevents the heat of the heater 30 from escaping to the lower part. The fourth block 217 includes an injection port 223 for injecting a buffer solution, an exhaust port 224 for discharging the solution after measurement, and a chamber 225 for accommodating a rotating body 226 of a motor 230 with a magnet attached to the center. 0227 where is drilled is the lower stand,
The central protrusion has an injection port 223 of the fourth block 217.
and holes 228 and 229 communicating with the outlet 224, respectively.
A hole 231 for attaching a motor 230 is drilled, and the reaction cell body is fixed to a base 232 with bolts 233. Reference numeral 0234 is a stirrer for stirring the mixture of the liquid to be measured and the buffer solution. The drain tube 235 is connected to a drain container (32 in FIG. 1), and is drained by a vacuum pump (33 in FIG. 1).

Next, the operation of the biological component analyzer of the present invention will be explained with reference to FIGS. 1 to 7. First, in FIG. 1, the heating device is preheated. 1
temperature controller 5, the measuring cell 25 is connected to the heater 30 and the second
temperature controller 34, turntable 19 with reaction container
The reaction vessel 21 is kept at a constant temperature by a heater and a third temperature controller (not shown in FIG. 1). The circulation pump 3 takes out the buffer solution from the tube contained in the buffer solution 2 in the buffer solution container 1 and supplies the buffer solution to the tube 6- which is spirally wound around the heating drum 4. The temperature of the buffer solution in the tube 6, which is spirally wound around the heating drum 4 by receiving heat from the heating drum 4, reaches a constant temperature at its end point X', and is returned to the buffer solution container 1. The buffer solution removed at this time is returned to the buffer solution 2 through the wall of the buffer solution container 1. As a result, the temperature of the buffer solution 2 in the buffer solution container 1 becomes constant. The first switching valve 9 connects a and b, and the first
cylinder 8 (after inhaling the buffer solution 2, the first switching valve 9 switches to the connection of b and c)
cylinder 8 transfers the buffer solution to the mouth of the first switching valve;
Dispense the buffer solution into C. The second switching valve 10 is a
' and C', and the third switching valve connects a'' and b.
'', whereby the buffer from the first cylinder 8 is supplied via a tube to the tube 7 which is helically wound around the heating drum 4. The buffer solution in the tube 7 is heated again and reaches a constant temperature at the end point Y of the tube, which is wound around the circumferential groove 29 of the measuring cell 25 (the measuring cell is also at a constant temperature). (so it is heated again to a constant temperature), and the measuring cell 25
Here, the buffer solution in the reaction chamber is stirred and kept at a constant temperature. (It is preferable to do this immediately after washing with a buffer solution) The 0th measurement liquid valve and the second switching valve are set to b and 0% a'.
By connecting and b' and discharging, the buffer solution flows out from the measuring liquid nozzle 17 and is washed.The waste liquid from the washing tank 24 is accumulated in the waste liquid tank 32, but the explanation of the operation is omitted because it is simple. do. The first nozzle then moves to position (2).The second cylinder 12 sucks in some air through the nozzle for the liquid to be measured.Next, the container 22 containing silicone oil and the reagent are placed. The first nozzles 15 are respectively inserted into the containers 23, and the fourth cylinder 14 inhales solicon oil into the silicone oil nozzle 16, and the second cylinder 12 inhales the reagent into the liquid to be measured nozzle 17. The second switching valve 10 is b'
After inhalation, the first nozzle 15 moves to the position (3), and the sample is inhaled from the sample container 20 by the second cylinder 12 through the nozzle 17 for the liquid to be measured. The first nozzle 15 moves to the position (4) where the reagent and sample are mixed to form a liquid to be measured, and the second cylinder 12 causes the nozzle 17 for the liquid to be measured to transfer the liquid to the reaction container. 21. After dispensing, the first nozzle (
5), and the silicone oil nozzle 16 discharges silicone oil onto the discharged liquid to be measured using the cylinder 14. The first nozzle 15 repeats this operation one after another by the first, second, and fourth cylinders 9, 12, and 14. During this time, the turntable 19 with reaction container rotates in the clockwise direction one step at a time. In other words, the movement of the first nozzle IS described above is one step, and in the next step, for example, when the first nozzle discharges the next liquid to be measured, the liquid to be measured is added to the reaction container in the previous step. Rotate so that the adjacent reaction container is directly below the nozzle 17 for the liquid to be measured at the +41 position (see Figures 1 and 5 for the operation of this turntable with a reaction container). (described in detail later). After the liquid to be measured has sufficiently reacted (after several steps), the reaction container containing the liquid to be measured will be directly below the position (6) of the second nozzle lr, so the second nozzle/lr will be The liquid to be measured is sucked into the third cylinder 73 (at this time, the third cylinder 73
switching pulp // brings C1l into the closed state). After inhalation, the second nozzle/nozzle moves to position (7), and the third cylinder discharges the liquid to be measured into the reaction chamber 21 via the injection passage 27 in the measurement cellco S. The operation of this second nozzle is also performed every single step in the same way as the operation of the first nozzle. As a result, the liquid to be measured that has entered the reaction chamber is mixed with the buffer solution and analyzed by the biological component analysis electrode 2B. After the measurement, the fourth switching pulp 37 switches and connects a and CI, operates the vacuum pump 33, and pours the solution in the reaction chamber 2g into the waste liquid tank 32.

Thereafter, as described above, the reaction chamber 2g is washed with the buffer using the first cylinder and filled. This action is also the second
The nozzle repeats each step.

The order is that after the solution in the reaction chamber 21 of the measurement cell 2S is discharged, it is washed, and the reaction chamber 21 is filled with a buffer solution. Next, the first
Nozzle a9 etc. is 0) → (2) → (3) → (4) → (5
) operates in the order of the positions. Next, the second nozzle /r etc. (
After operating in the order of 6)→(7), the biological components of the solution in the reaction chamber are analyzed. After operating these seven steps, the turntable with reaction container rotates seven steps. It is most desirable to repeat these steps. A more detailed operation of the measurement cell 2j will be described later.

Regarding the operations in FIG. 2, operations that overlap with those in FIG. 1 will be omitted for simplicity. After being converted into an electrical signal by the electrode 26 for biological component analysis in the measurement housing llr, the digital signal processed by the signal processing circuit 19 is input to the microcomputer and sent to the display device 39, printing device IO, and output terminal 41. In FIG. 3, the buffer solution flowing from the circulation pump enters the starting point It receives the heat of the heat transfer body 51 heated to a constant temperature by the vessel 53, is heated to a constant temperature, reaches the end point X' of the tube, and is returned to the buffer solution container. On the other hand, the heat transfer body 5 is heated to a constant temperature from the first cylinder 8.
1, it is heated to a constant temperature and reaches the end point Y' of the tube. The operation of the turntable with a reaction container will be explained with reference to FIGS. 4 and 5. First, a sample (for example, serum, etc.) is placed in the sample container 20. Rotating body (first and second fixed disks, 101.').

103, reaction container disc 102 and turntable main body 1
20) rotates clockwise, then the second
The detector 110 detects the hole 109 for initial position detection. When one hole 109 is detected, the controller (not shown) stops sending pulses to the pulse smoke 125 and stops the rotating body. That position becomes the starting point. Next, a measuring liquid nozzle (not shown) sucks the sample from the sample container 20, mixes it with the previously sucked reagent, and discharges it into the reaction container 21. Since the reaction container 21 is heated to a constant temperature by the heater 116, the sample and reagent are also kept at a constant temperature and react.The zero-order rotating body rotates clockwise and transfers the sample container next to the sample container to be measured. It will come directly below where the liquid nozzle was. To position the sample container 20, the control device sends a pulse to the pulse motor, and gear 124 and gear 1
23 to rotate the turntable clockwise to the first
When the detector 111 detects the small hole 108 for position detection, a detection signal is fed back to the control device, and the pulse motor 125 stops sending pulses to perform positioning. The positioned sample container 20 is subjected to the above-described operation by the nozzle. The above steps are repeated, and the sample and reagent ``-1-'' that have entered the reaction container 21 and reacted sufficiently are successively sucked in through another second nozzle (not shown) and discharged into the measurement cell to be exposed. The size of the reaction vessel 21 shown in FIG. 4 for measuring the liquid to be measured can be changed depending on the amount of the liquid to be measured, depending on the amount of the liquid to be measured.

To explain the operations in FIGS. 6 and 7, first, the buffer solution is injected through the tube 211 to the reaction chamber 28 and then to the middle of the large diameter part of the injection passage 27 of the second block 208.
Next, when the motor 230 is powered on, the magnet attached to the tip of the rotor 231 of the motor causes the stirrer 3 to
4 also rotates together with the rotor 231. Next, a nozzle containing the liquid to be measured is inserted into the nozzle-shaped injection passage 27 of the second block until its tip enters the reaction chamber, and the liquid to be measured is injected into the reaction chamber . The liquid to be measured and the buffer solution in the reaction chamber 28 are stirred and react with a predetermined electrode. For example, if the sample is glucose, the biological component analysis electrode 26,
Using an enzyme electrode as 210, 216, 218, a reaction of glucose oxidase glucose + 02 gluconolactone + H2O2 occurs, and the amount of oxygen consumed is measured with the oxygen electrode of the enzyme electrode at the electrode tip 219 near the immobilized glucose oxidase. . The enzyme electrode is connected to a signal processing circuit, etc., and the oxygen reduction amount and maximum oxygen consumption rate corresponding to the glucose concentration contained in the sample are measured. In addition to glucose, triglycerides, total cholesterol, and phospholipids are measured using an enzyme electrode known as an electrode for biological component analysis, making it possible to analyze multiple biological components at once. By selecting and combining appropriate samples, reagents, and electrodes for biological component analysis, it becomes possible to analyze a wider range of biological components. When sending the buffer solution to the reaction chamber in the measurement cell while always keeping the buffer solution inside at a constant temperature, the buffer solution can be sent at a constant temperature by heating it midway through. Furthermore, by providing a temperature control device outside the buffer solution container, the buffer solution in the buffer solution container can be easily replaced. Also, use a turntable with a reaction container (G)
By having a reaction container disc that is detachable from the turntable body, and by heating the reaction container of the reaction container disc to a constant temperature via a heater, the liquid to be measured can be sufficiently reacted.

Before the liquid to be measured is placed in the measurement cell and measured, the reaction container is preheated to the trowel measurement temperature, so when the liquid is placed in the measurement cell, it quickly reaches the measurement temperature and measurement errors are reduced. Further, since a reaction container of an appropriate size can be selected depending on the amount of the liquid to be measured and attached to the turntable body, it is possible to reduce the amount of the liquid to be measured. This makes it possible to downsize the entire device. Furthermore, when using the measurement cell described above, at least a part of the side surface of the reaction chamber is sloped in the shape of a cone, so it is possible to attach the electrode for biological component analysis diagonally to the main body, and the reaction chamber can be made smaller. , the amount of the mixed solution of the liquid to be measured and the buffer solution can be miniaturized. In this way, since the actual amount of the measuring liquid is limited to only the reaction chamber, the amount of the measuring liquid is always constant and highly accurate measurement is possible. In addition, since the electrode for biological component analysis can be installed diagonally with the tip facing downward, the solution in the reaction chamber will not leak when the electrode for biological component analysis is removed, and there is no need to take up a large space for removal. When a biological component analysis electrode that uses a liquid is used, air bubbles contained in the electrolysis can easily move, and detection can be performed satisfactorily. These effects mainly ensure that the temperature of buffer solutions, etc., is sufficiently controlled, and the amount of measurement solution in the reaction chamber in the measurement cell can be miniaturized, making it possible to apply it to biological component analysis where there is not much sample available (for example, for infants and infants). ), response speed to temperature is fast, measurement accuracy is improved,
This has the effect of making the entire device smaller.

The present invention is not limited to this, but as an application to an apparatus for analyzing biological components, in FIG.
If you use all oxygen-impermeable tubes, the injection passage of the measurement cell 25 will be sealed with liquid. Therefore, it can be applied to samples that do not want to come into contact with oxygen.

[Brief explanation of drawings]

Figure 1 is a schematic diagram showing the entire biological component analyzer of the present invention, Figure 2 is a block diagram of Figure 1 with the addition of an electrical system, and Figure 3 is a circulation pump used in the present invention. A sectional view of an embodiment showing the main parts of a buffer solution temperature control device excluding
Fig. 4 is a top view of an example showing the main parts of a turntable with a reaction vessel used in the present invention, Fig. 5 is a sectional view of Fig. 4 seen from the side, and Fig. 6 is a measurement used in the present invention. FIG. 7 is a top view of one embodiment showing the essential parts of the cell, and is a sectional view of FIG. 6 viewed from the side. 1: Container for buffer solution 2: Buffer solution 4: Heating drum 5: 10th) Temperature controller 6: Heating drum 1 spirally wound tube 7: Heating drum 1 spirally wound tube 8: First cylinder. 12: Second cylinder 13: Third cylinder 14: Fourth cylinder 15: First nozzle 1 also = Silicon oil nozzle 17: Measured liquid nozzle 18: Second nozzle 1-9 Two reaction vessels Turntable 20: Sample container 21 containing a sample: Reaction container 23: Reagent container 25 containing a reagent: Measurement cell 26: Electrode 27 for biological component analysis: Injection passage 28: Reaction chamber 102: Reaction container Disc 116: Heater//Za: Lubricating and heat resistant membrane Patent applicant: Oriental Yeast Industry Co., Ltd.

Claims (1)

[Claims] (1) A temperature control device that keeps the buffer solution in the buffer container at a constant temperature, a cylinder that sends the buffer solution to the reaction chamber in the measurement cell, a turntable that holds the sample, and the sample and reagent. A mixing conveying means for mixing and conveying the mixed liquid, and a cylinder for transporting the liquid to be measured mixed by the mixing conveying means.
In the apparatus, the buffer is injected into the reaction chamber in the measurement cell through the injection passage, mixed with the buffer, and the mixed liquid is analyzed for biological components using an electrode for biological component analysis. A temperature control device for heating to a constant temperature using a circulation pump outside the measurement cell, and a reaction chamber in the measurement cell have a shape in which at least a part of the side surface forms an acute angle with the lower ml, and the reaction chamber side of the injection passage. and an injection passageway configured to prevent the solution in the injection passageway above the narrow injection passageway on the reaction chamber side from being agitated due to the reaction in the reaction chamber. Biological component analyzer a mixing and transporting means for mixing and transporting a sample and a reagent; and a cylinder for injecting a liquid to be measured mixed by the mixing and transporting means into a reaction chamber in the measurement cell through an injection passage and mixing it with the buffer solution. In an apparatus for analyzing the biological components of the mixed liquid using an electrode for biological component analysis, the loose-fitting liquid is heated to a constant temperature using a circulation pump outside the buffer solution container, and a temperature control device is used to heat the sample to a constant temperature. It has a reaction vessel disk which is removably attached to the inside of the holding vessel and has a large number of reaction vessels, and furthermore, it has a heater for heating the reaction vessels of the reaction vessel disk to a constant temperature and a heater with good lubricity on its upper part. The reaction chamber in the measurement cell has a shape in which at least a part of the side surface forms an acute angle with the lower surface, and the injection passage is narrowed on the reaction chamber side, so that the reaction chamber in the measurement cell is A biological component analyzer comprising a measurement cell consisting of an injection passageway in which a solution in an upper injection passageway is prevented from being stirred by a reaction than a narrow injection passageway on the side of the reaction chamber.