JPS5826319B2 - コウソサヨウニヨル オンドヘンカノソクテイホウホウホウ オヨビ ソウチ - Google Patents
コウソサヨウニヨル オンドヘンカノソクテイホウホウホウ オヨビ ソウチInfo
- Publication number
- JPS5826319B2 JPS5826319B2 JP50068120A JP6812075A JPS5826319B2 JP S5826319 B2 JPS5826319 B2 JP S5826319B2 JP 50068120 A JP50068120 A JP 50068120A JP 6812075 A JP6812075 A JP 6812075A JP S5826319 B2 JPS5826319 B2 JP S5826319B2
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- detector
- accommodating
- container
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000790 Enzymes Proteins 0.000 claims description 41
- 102000004190 Enzymes Human genes 0.000 claims description 41
- 239000000758 substrate Substances 0.000 claims description 17
- 230000008859 change Effects 0.000 claims description 15
- 239000011159 matrix material Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000009529 body temperature measurement Methods 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 35
- 239000000243 solution Substances 0.000 description 17
- 102000004142 Trypsin Human genes 0.000 description 12
- 108090000631 Trypsin Proteins 0.000 description 12
- 239000011521 glass Substances 0.000 description 11
- 239000012588 trypsin Substances 0.000 description 11
- 238000005259 measurement Methods 0.000 description 8
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 6
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000006911 enzymatic reaction Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 239000005662 Paraffin oil Substances 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 2
- 125000003282 alkyl amino group Chemical group 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- YQDHCCVUYCIGSW-LBPRGKRZSA-N ethyl (2s)-2-benzamido-5-(diaminomethylideneamino)pentanoate Chemical compound NC(=N)NCCC[C@@H](C(=O)OCC)NC(=O)C1=CC=CC=C1 YQDHCCVUYCIGSW-LBPRGKRZSA-N 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000005588 protonation Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000002076 thermal analysis method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 101100380699 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) atp-8 gene Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- TTWYZDPBDWHJOR-IDIVVRGQSA-L adenosine triphosphate disodium Chemical compound [Na+].[Na+].C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O TTWYZDPBDWHJOR-IDIVVRGQSA-L 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- -1 avirase Proteins 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 238000010931 ester hydrolysis Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
- G01N25/20—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
- G01N25/48—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
- G01N25/4873—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation for a flowing, e.g. gas sample
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Combustion & Propulsion (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7407494A SE401037B (sv) | 1974-06-07 | 1974-06-07 | Sett att genom metning av temperaturforendringar bestemma koncentrationen av ett emne som under paverkan av ett enzym avger eller upptar verme, samt apparat for settets utforande |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5111498A JPS5111498A (en) | 1976-01-29 |
| JPS5826319B2 true JPS5826319B2 (ja) | 1983-06-02 |
Family
ID=20321354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50068120A Expired JPS5826319B2 (ja) | 1974-06-07 | 1975-06-04 | コウソサヨウニヨル オンドヘンカノソクテイホウホウホウ オヨビ ソウチ |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4021307A (ref) |
| JP (1) | JPS5826319B2 (ref) |
| FR (1) | FR2274045A1 (ref) |
| GB (1) | GB1500388A (ref) |
| SE (1) | SE401037B (ref) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1113361B (it) * | 1979-05-08 | 1986-01-20 | Italfarmaco Spa | Reattore a flusso ad enzimi immobilizzati su matrici solide a superficie piana |
| US4551425A (en) * | 1982-09-28 | 1985-11-05 | Trustees Of The University Of Pennsylvania | Pyroelectric gas sensor |
| JPH06105261B2 (ja) * | 1984-03-05 | 1994-12-21 | 株式会社東芝 | 濃度勾配測定装置 |
| JPS622144A (ja) * | 1985-06-27 | 1987-01-08 | Unitika Ltd | 温度滴定の方法および装置 |
| JPH0612347B2 (ja) * | 1985-12-26 | 1994-02-16 | 正次郎 伊藤 | 精密熱量計 |
| US4829003A (en) * | 1986-08-28 | 1989-05-09 | Arney Jr Lawrence H | Pyroelectric enzyme detector |
| US4935345A (en) * | 1987-04-07 | 1990-06-19 | Arizona Board Of Regents | Implantable microelectronic biochemical sensor incorporating thin film thermopile |
| WO1999039629A1 (en) | 1998-02-04 | 1999-08-12 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Chemical sensors having microflow systems |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6703246B1 (en) * | 1999-07-06 | 2004-03-09 | The Dow Chemical Company | Thermal method and apparatus |
| AU6103800A (en) * | 1999-07-19 | 2001-02-05 | Althexis Company, Inc., The | Thermo-chemical sensors and uses thereof |
| US8262989B2 (en) * | 2000-07-21 | 2012-09-11 | Senzime Ab (Publ.) | Micro-calorimeter apparatus |
| US7833800B2 (en) * | 2002-04-01 | 2010-11-16 | Palo Alto Research Center Incorporated | Thermal sensing with bridge circuitry |
| US20060121547A1 (en) * | 2004-12-03 | 2006-06-08 | Mcintire Mark | Diffusion layer for an enzyme-based sensor application |
| CN104569039A (zh) * | 2014-12-05 | 2015-04-29 | 苏州国环环境检测有限公司 | 采用酶反应器检测有机磷农药残留的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3542662A (en) * | 1967-04-18 | 1970-11-24 | Du Pont | Enzyme electrode |
| US3847741A (en) * | 1972-10-02 | 1974-11-12 | Cpc International Inc | Temperature-programmed process for the production of levulose-bearing syrups |
| US3878049A (en) * | 1973-04-03 | 1975-04-15 | Massachusetts Inst Technology | Biochemical temperature-sensitive probe and method for measuring reactant concentrations thereof |
-
1974
- 1974-06-07 SE SE7407494A patent/SE401037B/xx not_active IP Right Cessation
-
1975
- 1975-06-02 GB GB23850/75A patent/GB1500388A/en not_active Expired
- 1975-06-04 JP JP50068120A patent/JPS5826319B2/ja not_active Expired
- 1975-06-06 US US05/584,595 patent/US4021307A/en not_active Expired - Lifetime
- 1975-06-06 FR FR7517767A patent/FR2274045A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5111498A (en) | 1976-01-29 |
| SE7407494L (sv) | 1975-12-08 |
| GB1500388A (en) | 1978-02-08 |
| US4021307A (en) | 1977-05-03 |
| FR2274045A1 (fr) | 1976-01-02 |
| FR2274045B1 (ref) | 1979-03-23 |
| DE2524838A1 (de) | 1975-12-18 |
| DE2524838B2 (de) | 1977-02-03 |
| SE401037B (sv) | 1978-04-17 |
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