JPS5817638Y2 - centrifugal sedimentation tube - Google Patents

Description

[Detailed description of the invention] This invention relates to a centrifugal sedimentation tube used in a separation ultracentrifuge.
In particular, it facilitates securing the separation capacity and fractionating the supernatant.

A conventional centrifuge tube is shown in FIGS. 1-4 and FIG.

1 and 2 show a centrifugal sedimentation tube 1 in which the inner wall of the body portion 1a includes a mouth portion 11 and is straight, and shows a stationary state, an operating state, and an operating state, respectively.

In this centrifugal sedimentation tube, the sedimentation tube insertion hole of the rotor is close to horizontal (
・Although it is advantageous, in recent centrifugation, in order to shorten sedimentation time, it is often used in a nearly vertical (・) state.

Therefore, such a centrifugal sedimentation tube has a small separation capacity and is inconvenient.

FIGS. 3 and 4 show the auf-fringed centrifugal sedimentation tube 4 in a stationary state and in operation, respectively.

Although this centrifugal sedimentation tube 4 can secure a slightly larger separation capacity than the above-mentioned sedimentation tube, it should be improved.

Furthermore, as shown in FIG. 17, this centrifugal sedimentation tube had the disadvantage that when fractionating a supernatant liquid, it was difficult to remove the separated sample because there was a shoulder 12 around the entire circumference.

In the same figure, 2 indicates the liquid level while it is stationary, 3 indicates the liquid level during operation, and 8 indicates the separated sample.

The object of the present invention is to eliminate the above-mentioned drawbacks of the conventional centrifugal sedimentation tube and to further improve operability.

Examples of the present invention are shown in FIGS. 5, 6, 8, and 9 to 12.

In the figure, at °C, the mouth 13 of the centrifugal sedimentation tube 5 biases the mouth inner wall 14 on the outside in the direction of centrifugal force toward the central axis XX of the body 5a, and on the other hand, the mouth inner wall 15 on the inside in the direction of centrifugal force is formed straight so as to be on an extension line of the inner wall 18 of the body on the inside in the direction of centrifugal force.

A sample is provided on the inside of the opening 13 in the direction of centrifugal force to facilitate fractionation of the supernatant after separation and to position the centrifugal sedimentation tube 5 when inserting it into the sedimentation tube insertion hole 16 of the rotating body 10. A guide section 1 is provided.

Reference numeral 6 indicates a shoulder formed by biasing the inner wall 14 of the mouth portion.

According to the embodiment of the present invention described above, a large separation capacity can be ensured, and the supernatant liquid after separation can be easily fractionated.

As shown in FIG. 13, the shape of the mouth portion 13 of the centrifugal sedimentation tube 5 is not limited to a circle, and various shapes are possible, and it is desirable that the length 11 of the shoulder portion is as large as possible.

Similar to what has been described above, a centrifugal sedimentation tube 5 as shown in FIGS. 14 and 15 can be considered.

In the case of FIG. 15, positioning becomes a problem, but this can be easily solved by providing a positioning protrusion on the cap.

According to the present invention, separation capacity can be ensured, and at the same time, the supernatant liquid after separation can be easily fractionated.

Particularly effective for large-capacity centrifugal sedimentation tubes.

[Brief explanation of the drawing]

FIG. 1 and FIG. 2 are longitudinal cross-sectional views showing a conventional centrifugal sedimentation tube in a stationary state and an operating state, respectively. FIGS. 3 and 4 are longitudinal cross-sectional views showing a conventional oaf-fringed centrifugal sedimentation tube in a stationary state and in operation, respectively. FIGS. 5 and 6 are longitudinal cross-sectional views showing an embodiment of the present invention in a stationary state and an operating state, respectively. FIG. 7 is a vertical cross-sectional view showing the fractionation of the supernatant liquid in a conventional oaf-fringe type centrifugal sedimentation tube, and FIG. 8 is a vertical cross-sectional view showing the fractionation of the supernatant liquid in an embodiment of the present invention. FIG. 9 is a longitudinal cross-sectional view of a rotating body housing the settling tube of the present invention, and FIG. 10 is a plan view thereof. 11 and 12 respectively show a vertical sectional view and a plan view of one embodiment of the present invention, and FIGS. 13 to 15 show other embodiments of the present invention, with FIG. 13 being a plan view. Figure, 1st
4 and 15 are longitudinal sectional views, respectively. 5 is a centrifugal sedimentation tube, 5a is a body part, 6 is a shoulder part, 13 is a mouth part,
14 is the inner wall of the mouth, 15 is the inner wall of the mouth, and 18 is the inner wall of the body.

Claims (1)

[Scope of utility model registration request] In a centrifugal sedimentation tube used in a separation ultracentrifuge, which has an opening at the upper end, the inner wall of the opening on the outside in the direction of centrifugal force is biased in a direction approaching the center ridge line of the sedimentation tube, and the inner wall of the opening on the outside in the direction of centrifugal force is biased toward the center ridge line of the sedimentation tube, while the inner wall of the opening on the outside in the direction of centrifugal force This is a centrifugal sedimentation tube in which the inner wall of the inner mouth is formed straight so that it is located on an extension of the inner wall of the body of the sedimentation tube.