JPS5794650A - Method and apparatus for concentrating dispensed sample for liquid chromatography - Google Patents

Method and apparatus for concentrating dispensed sample for liquid chromatography

Info

Publication number
JPS5794650A
JPS5794650A JP17025480A JP17025480A JPS5794650A JP S5794650 A JPS5794650 A JP S5794650A JP 17025480 A JP17025480 A JP 17025480A JP 17025480 A JP17025480 A JP 17025480A JP S5794650 A JPS5794650 A JP S5794650A
Authority
JP
Japan
Prior art keywords
liquid
column
aimed component
aimed
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17025480A
Other languages
Japanese (ja)
Inventor
Akimitsu Wada
Hidechika Hayashi
Tsutomu Hashimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Toyo Soda Manufacturing Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Soda Manufacturing Co Ltd filed Critical Toyo Soda Manufacturing Co Ltd
Priority to JP17025480A priority Critical patent/JPS5794650A/en
Publication of JPS5794650A publication Critical patent/JPS5794650A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/08—Preparation using an enricher

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

PURPOSE:To concentrate aimed component automatically in high efficiency, by adding the regulation liquid for concentration to the liquid containing the aimed component which is refined by a liquid chromatograph apparatus, sending said liquid to a column for concentration and then, adsorbing the aimed component and after that, taking out the aimed component in high concentration by using elution liquid and regenerating the column. CONSTITUTION:A regulation liquid B2 is added to a liquid containing aimed component which is refined by a liquid chromatograph apparatus in order to regulate in the condition of liquid of absorbing the aimed component to a column for concentration C2 by a pump 2 and said liquid flows to the column C2. The fact that the aimed component is adsorbed to the comumn 2, is detected by a detector D2. After the aimed component is adsorbed sufficiently, an elution liquid B3 is sent to the column 2 by operating a changeover valve V3, a pump P3 and a changeover valve V2 and the aimed component is eluted. A highly concentrated liquid is then collected in a fraction collector F. Next, the column C2 is regenerated by a regeneration liquid B4 by operating the valve V3, V2. Thus, the concentration of the aimed component is automatically carried out effectively.
JP17025480A 1980-12-04 1980-12-04 Method and apparatus for concentrating dispensed sample for liquid chromatography Pending JPS5794650A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17025480A JPS5794650A (en) 1980-12-04 1980-12-04 Method and apparatus for concentrating dispensed sample for liquid chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17025480A JPS5794650A (en) 1980-12-04 1980-12-04 Method and apparatus for concentrating dispensed sample for liquid chromatography

Publications (1)

Publication Number Publication Date
JPS5794650A true JPS5794650A (en) 1982-06-12

Family

ID=15901525

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17025480A Pending JPS5794650A (en) 1980-12-04 1980-12-04 Method and apparatus for concentrating dispensed sample for liquid chromatography

Country Status (1)

Country Link
JP (1) JPS5794650A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62229065A (en) * 1986-03-31 1987-10-07 Shimadzu Corp Analysis of low-molecular component in high-molecular substance and device therefor
JPH02122260A (en) * 1988-10-31 1990-05-09 Shimadzu Corp Preparative liquid chromatograph

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5042895A (en) * 1973-08-20 1975-04-18
JPS50127697A (en) * 1974-03-27 1975-10-07

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5042895A (en) * 1973-08-20 1975-04-18
JPS50127697A (en) * 1974-03-27 1975-10-07

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62229065A (en) * 1986-03-31 1987-10-07 Shimadzu Corp Analysis of low-molecular component in high-molecular substance and device therefor
JPH02122260A (en) * 1988-10-31 1990-05-09 Shimadzu Corp Preparative liquid chromatograph

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