JPS5788196A - Production of oligonucleotides - Google Patents
Production of oligonucleotidesInfo
- Publication number
- JPS5788196A JPS5788196A JP16192580A JP16192580A JPS5788196A JP S5788196 A JPS5788196 A JP S5788196A JP 16192580 A JP16192580 A JP 16192580A JP 16192580 A JP16192580 A JP 16192580A JP S5788196 A JPS5788196 A JP S5788196A
- Authority
- JP
- Japan
- Prior art keywords
- formula
- condensation reaction
- nucleotide
- compound
- combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Saccharide Compounds (AREA)
Abstract
PURPOSE: The condensation reaction of nucleotides is effected in the pressence of a specific combination of condensing agents to produce oligopeptides used in gene recombination in high yield.
CONSTITUTION: The condensation reaction between a nucleotide of formulaI(N1, N2 are residue of thymine, N-benzoyldeoxycytosine, N-benzoyldeoxyadenine, N- iso-butyryldeoxyguanine; T is dimethoxytrityl; X is cyanoethyl, P- or o-chlorophenyl, trichloroethyl; m is 0, 1) and another nucleotide of formula II or III (N3, N4 is N1; B is benzoyl, anisoyl; X is Y; n is 0, 1) is conducted in the presence of a combination of (A) mesitylenesulfonyltriazole or triisopropylbenzenesulfonyl nitroimidazole with (B) 1-methylimidazole or 4-dimethylaminopyridine to give the objective compound of formula IV or V. The molar ratio of A:B: the compound of formulaIis (1W4):(2W8):1.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16192580A JPS5788196A (en) | 1980-11-19 | 1980-11-19 | Production of oligonucleotides |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16192580A JPS5788196A (en) | 1980-11-19 | 1980-11-19 | Production of oligonucleotides |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5788196A true JPS5788196A (en) | 1982-06-01 |
Family
ID=15744634
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16192580A Pending JPS5788196A (en) | 1980-11-19 | 1980-11-19 | Production of oligonucleotides |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5788196A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0415235A2 (en) * | 1989-08-29 | 1991-03-06 | Gesellschaft für Biotechnologische Forschung mbH (GBF) | Process for carefully esterification of a carboxylic acid with an alcohol component |
-
1980
- 1980-11-19 JP JP16192580A patent/JPS5788196A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0415235A2 (en) * | 1989-08-29 | 1991-03-06 | Gesellschaft für Biotechnologische Forschung mbH (GBF) | Process for carefully esterification of a carboxylic acid with an alcohol component |
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