JPS5788196A - Production of oligonucleotides - Google Patents

Production of oligonucleotides

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Publication number
JPS5788196A
JPS5788196A JP16192580A JP16192580A JPS5788196A JP S5788196 A JPS5788196 A JP S5788196A JP 16192580 A JP16192580 A JP 16192580A JP 16192580 A JP16192580 A JP 16192580A JP S5788196 A JPS5788196 A JP S5788196A
Authority
JP
Japan
Prior art keywords
formula
condensation reaction
nucleotide
compound
combination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP16192580A
Other languages
Japanese (ja)
Inventor
Toshiaki Wakabayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eisai Co Ltd
Original Assignee
Eisai Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai Co Ltd filed Critical Eisai Co Ltd
Priority to JP16192580A priority Critical patent/JPS5788196A/en
Publication of JPS5788196A publication Critical patent/JPS5788196A/en
Pending legal-status Critical Current

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Abstract

PURPOSE: The condensation reaction of nucleotides is effected in the pressence of a specific combination of condensing agents to produce oligopeptides used in gene recombination in high yield.
CONSTITUTION: The condensation reaction between a nucleotide of formulaI(N1, N2 are residue of thymine, N-benzoyldeoxycytosine, N-benzoyldeoxyadenine, N- iso-butyryldeoxyguanine; T is dimethoxytrityl; X is cyanoethyl, P- or o-chlorophenyl, trichloroethyl; m is 0, 1) and another nucleotide of formula II or III (N3, N4 is N1; B is benzoyl, anisoyl; X is Y; n is 0, 1) is conducted in the presence of a combination of (A) mesitylenesulfonyltriazole or triisopropylbenzenesulfonyl nitroimidazole with (B) 1-methylimidazole or 4-dimethylaminopyridine to give the objective compound of formula IV or V. The molar ratio of A:B: the compound of formulaIis (1W4):(2W8):1.
COPYRIGHT: (C)1982,JPO&Japio
JP16192580A 1980-11-19 1980-11-19 Production of oligonucleotides Pending JPS5788196A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16192580A JPS5788196A (en) 1980-11-19 1980-11-19 Production of oligonucleotides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16192580A JPS5788196A (en) 1980-11-19 1980-11-19 Production of oligonucleotides

Publications (1)

Publication Number Publication Date
JPS5788196A true JPS5788196A (en) 1982-06-01

Family

ID=15744634

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16192580A Pending JPS5788196A (en) 1980-11-19 1980-11-19 Production of oligonucleotides

Country Status (1)

Country Link
JP (1) JPS5788196A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0415235A2 (en) * 1989-08-29 1991-03-06 Gesellschaft für Biotechnologische Forschung mbH (GBF) Process for carefully esterification of a carboxylic acid with an alcohol component

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0415235A2 (en) * 1989-08-29 1991-03-06 Gesellschaft für Biotechnologische Forschung mbH (GBF) Process for carefully esterification of a carboxylic acid with an alcohol component

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