JPS57200400A - Desamidosecretin gene and plasmid - Google Patents

Desamidosecretin gene and plasmid

Info

Publication number
JPS57200400A
JPS57200400A JP8460481A JP8460481A JPS57200400A JP S57200400 A JPS57200400 A JP S57200400A JP 8460481 A JP8460481 A JP 8460481A JP 8460481 A JP8460481 A JP 8460481A JP S57200400 A JPS57200400 A JP S57200400A
Authority
JP
Japan
Prior art keywords
gene
desamidosecretin
plasmid
fragment
blocks
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP8460481A
Other languages
Japanese (ja)
Inventor
Masanori Suzuki
Tetsuo Miyake
Kenichi Miyoshi
Shinichiro Sumi
Akira Hasegawa
Tsutomu Nishizawa
Toru Fuwa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wakunaga Pharmaceutical Co Ltd
Original Assignee
Wakunaga Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wakunaga Pharmaceutical Co Ltd filed Critical Wakunaga Pharmaceutical Co Ltd
Priority to JP8460481A priority Critical patent/JPS57200400A/en
Priority to CH3267/82A priority patent/CH659826A5/en
Priority to DE19823220333 priority patent/DE3220333A1/en
Priority to IT21628/82A priority patent/IT1157277B/en
Priority to GB08215998A priority patent/GB2103220B/en
Priority to FR8209607A priority patent/FR2508928A1/en
Publication of JPS57200400A publication Critical patent/JPS57200400A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/645—Secretins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

NEW MATERIAL:A duplex polydeoxyribonucleotide containing a structural gene exhibiting 27-desamidosecretin. USE:A plasmid for massproducing inexpensively and quickly 27-desamidosecretin through genetic engineering, exhibiting a promoting action of the secretion of pencreatic juice by intravenous injection. PROCESS:A gene of base arrangement comprising a bond of the blocks A, B, and C is designed, each fragment S-1-S-16 is synthesized by a triester method preferably by solid phase method, purified and isolated by a reversed phase column. The 5'-hydroxyl group of each fragment is phosphorylated, bonded successively with DNA ligase in a buffer solution to form the blocks A, B, and C, and these are linked to give a structural gene. This gene is integrated in a vector plasmid such as the recognition site PstI of pBR322.
JP8460481A 1981-06-02 1981-06-02 Desamidosecretin gene and plasmid Pending JPS57200400A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP8460481A JPS57200400A (en) 1981-06-02 1981-06-02 Desamidosecretin gene and plasmid
CH3267/82A CH659826A5 (en) 1981-06-02 1982-05-27 PROCESS FOR OBTAINING A MICROORGANISM TRANSFORMED BY A PLASMID COMPRISING A GENE OF 27-DESAMIDOSECRETINE STRUCTURE.
DE19823220333 DE3220333A1 (en) 1981-06-02 1982-05-28 27-Deamidosecretin and process for preparing it by recombinant DNA technology
IT21628/82A IT1157277B (en) 1981-06-02 1982-06-01 27-DEAMIDOSECRETINA AND PROCEDURE FOR ITS PRODUCTION BY THE RECOMBINANT DNA TECHNIQUE
GB08215998A GB2103220B (en) 1981-06-02 1982-06-01 27-desamidosecretin and production of same through recombinant dna technique
FR8209607A FR2508928A1 (en) 1981-06-02 1982-06-02 27-DESAMIDOSECRETINE, PREPARATION THEREOF BY RECOMBINANT DNA TECHNIQUE, AS WELL AS TRANSFORMED ESCHERICHIA COLI STRAIN, POLYDESOXYRIBONUCLEOTIDES WITH TWO CHAINS AND PLASMIDS USED IN THIS PREPARATION

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8460481A JPS57200400A (en) 1981-06-02 1981-06-02 Desamidosecretin gene and plasmid

Publications (1)

Publication Number Publication Date
JPS57200400A true JPS57200400A (en) 1982-12-08

Family

ID=13835281

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8460481A Pending JPS57200400A (en) 1981-06-02 1981-06-02 Desamidosecretin gene and plasmid

Country Status (1)

Country Link
JP (1) JPS57200400A (en)

Similar Documents

Publication Publication Date Title
Rink et al. A large fragment approach to DNA synthesis: total synthesis of a gene for the protease inhibitor eglin c from the leech Hirudo medicinalis and its expression in E. coli
EP0043980B1 (en) Mature human leukocyte interferon a, process for its microbial preparation, intermediates therefor and compositions containing it.
Gray et al. Expression of human immune interferon cDNA in E. coli and monkey cells
CA1341569C (en) Microbial production of human fibroblast interferon
DE3050725C2 (en)
JP2557053B2 (en) Tumor necrosis factor purification, production and use
EP0077670A2 (en) Human immune interferon
DE3280400D1 (en) OLIGONUCLEOTIDES MEDICINE AND THEIR PRODUCTION METHOD.
EP0192392A3 (en) Insulin receptor, its purification, its production in recombinant cells, dna therefor, antibodies to insulin receptor and their preparation, and pharmaceutical compositions containing them
EP0502892A4 (en) The human c3b/c4b receptor (cr1)
EP0118808B1 (en) Purification of interferon
FI103049B1 (en) Hybrid DNA molecule, microorganism transformed with this molecule and process for producing a fibronectin binding protein
EP0411031A4 (en) The human c3b/c4b receptor (cr1)
US4801685A (en) Microbial production of mature human leukocyte interferon K and L
US5087613A (en) Hirudin variants
NZ211827A (en) Production of polypeptides having interferon activity, polypeptide compositions and dna
IL86086A (en) Fusion protein with activities of interleukin-2 and gm-csf and pharmaceutical compositions containing said protein
DE286239T1 (en) Production and purification of a protein fused to a binding protein.
DE3781217T2 (en) PREPARATION OF RELATED POLYPEPTIDES OF THE BINDING FACTOR.
US5026639A (en) Method to improve mRNA translation and use thereof for production of platelet factor-4
CA2020656A1 (en) Plasmids, their preparation and their use in the manufacture of a plasminogen activator
FI971100A0 (en) Process for producing recombinant DNA proteins, plasmids and modified cells
JPS57200400A (en) Desamidosecretin gene and plasmid
IE872160L (en) Recombinant immune interferon fragments
US4810645A (en) Microbial production of mature human leukocyte interferon K and L