JPS57182650A - Detection method for gene by oligoliponucleotide - Google Patents

Detection method for gene by oligoliponucleotide

Info

Publication number
JPS57182650A
JPS57182650A JP56067758A JP6775881A JPS57182650A JP S57182650 A JPS57182650 A JP S57182650A JP 56067758 A JP56067758 A JP 56067758A JP 6775881 A JP6775881 A JP 6775881A JP S57182650 A JPS57182650 A JP S57182650A
Authority
JP
Japan
Prior art keywords
gene
arrangement
basic arrangement
cytidine
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56067758A
Other languages
Japanese (ja)
Other versions
JPS64663B2 (en
Inventor
Isao Uchida
Masato Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP56067758A priority Critical patent/JPS57182650A/en
Publication of JPS57182650A publication Critical patent/JPS57182650A/en
Publication of JPS64663B2 publication Critical patent/JPS64663B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To detect even a gene having an amino acid arrangement by the synthesis of a few kinds of oligo nucleotide, by synthesizing oligouncleotide having basic arrangement corresponding to <=4 amino acids and using it as a probe. CONSTITUTION:Oligoliponuclueotide having basic arrangement CCXCCXCC, etc. [X shows cytidine (C), uridine (U) and adenosine (A)] whch is capable of forming strong hybrid (complex of DNA and RNA) such as guanosine (G), cytidine (C) and having the basic arrangement correspondig to 3-4 amino acids of a gene, is synthized arranging eight base at most by using an enzymatic method. The tip 5' of this synthesized oligolipouncleotide labeled by radioactive <32>P. Further, the gene coding specified amino acid arrangement i. e. specified protein, can be detected by using said labeled oligolipounucleotide. Thus, the useful gene is utilized by cloning.
JP56067758A 1981-05-06 1981-05-06 Detection method for gene by oligoliponucleotide Granted JPS57182650A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56067758A JPS57182650A (en) 1981-05-06 1981-05-06 Detection method for gene by oligoliponucleotide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56067758A JPS57182650A (en) 1981-05-06 1981-05-06 Detection method for gene by oligoliponucleotide

Publications (2)

Publication Number Publication Date
JPS57182650A true JPS57182650A (en) 1982-11-10
JPS64663B2 JPS64663B2 (en) 1989-01-09

Family

ID=13354153

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56067758A Granted JPS57182650A (en) 1981-05-06 1981-05-06 Detection method for gene by oligoliponucleotide

Country Status (1)

Country Link
JP (1) JPS57182650A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06100605B2 (en) * 1984-04-05 1994-12-12 ハワ−ド フロ−リ インステイチユ−ト オブ イクスペリメンタル フイジオロジ アンド メデイスン Hybridization histochemistry
US5413908A (en) * 1984-11-12 1995-05-09 Lister Institute Of Preventive Medicine Method of characterizing genomic DNA by reference to a genetic variable

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU659573A1 (en) * 1977-05-31 1979-04-30 Институт биологической физики АН СССР Spin-labelled derivatives of oligoribonucleotides as spin probes for investigating mechanism of effect of ferments and method of obtaining same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU659573A1 (en) * 1977-05-31 1979-04-30 Институт биологической физики АН СССР Spin-labelled derivatives of oligoribonucleotides as spin probes for investigating mechanism of effect of ferments and method of obtaining same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06100605B2 (en) * 1984-04-05 1994-12-12 ハワ−ド フロ−リ インステイチユ−ト オブ イクスペリメンタル フイジオロジ アンド メデイスン Hybridization histochemistry
US5413908A (en) * 1984-11-12 1995-05-09 Lister Institute Of Preventive Medicine Method of characterizing genomic DNA by reference to a genetic variable

Also Published As

Publication number Publication date
JPS64663B2 (en) 1989-01-09

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