WO1994012518A3 - Reagent for coupling various substances to nucleic acids, and a method of preparing the reagent - Google Patents

Reagent for coupling various substances to nucleic acids, and a method of preparing the reagent Download PDF

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Publication number
WO1994012518A3
WO1994012518A3 PCT/DE1993/001101 DE9301101W WO9412518A3 WO 1994012518 A3 WO1994012518 A3 WO 1994012518A3 DE 9301101 W DE9301101 W DE 9301101W WO 9412518 A3 WO9412518 A3 WO 9412518A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
nucleic acids
ribooligonucleotide
preparing
rna
Prior art date
Application number
PCT/DE1993/001101
Other languages
German (de)
French (fr)
Other versions
WO1994012518A2 (en
Inventor
Gabor Igloi
Original Assignee
Gabor Igloi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gabor Igloi filed Critical Gabor Igloi
Publication of WO1994012518A2 publication Critical patent/WO1994012518A2/en
Publication of WO1994012518A3 publication Critical patent/WO1994012518A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The invention concerns a ribooligonucleotide of the formula I or II, in which R1 is a hydroxyl or phosphate group or at least one 3', 5'-nucleotide bisphosphate; R2 is a nucleobase; R3 is the molecule to be coupled to the nucleic acid and R4 is a hydrogen atom or a nucleoside, m being a number between 1 and 100, in particular between 2 and 5; n1 being either 0 or a number between 1 and 30, in particular 1, and n2 being either 0 or a number between 1 and 30, in particular 1. The ribooligonucleotide is suitable for use in coupling marker compounds, in particular fluorescent markers but also proteins, in particular nucleases, to nucleic acids, using RNA ligases as catalysts. This modified ribooligonucleotide can be used for (a) hybridization processes, (b) investigations of the structure of RNA, (c) studies of the interactions between proteins and RNA or (d) particular modifications of nucleic acids.
PCT/DE1993/001101 1992-11-25 1993-11-19 Reagent for coupling various substances to nucleic acids, and a method of preparing the reagent WO1994012518A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19924239531 DE4239531A1 (en) 1992-11-25 1992-11-25 Reagent for coupling various substances to nucleic acids and process for its production
DEP4239531.3 1992-11-25

Publications (2)

Publication Number Publication Date
WO1994012518A2 WO1994012518A2 (en) 1994-06-09
WO1994012518A3 true WO1994012518A3 (en) 1994-07-21

Family

ID=6473567

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1993/001101 WO1994012518A2 (en) 1992-11-25 1993-11-19 Reagent for coupling various substances to nucleic acids, and a method of preparing the reagent

Country Status (2)

Country Link
DE (1) DE4239531A1 (en)
WO (1) WO1994012518A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19954934A1 (en) * 1999-11-16 2001-05-17 Otto S Wolfbeis Process for solubilizing optical markers
EP1136569A3 (en) 2000-03-24 2004-01-28 Bayer Corporation Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof
US7541144B2 (en) 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US7718365B2 (en) 2005-07-09 2010-05-18 Agilent Technologies, Inc. Microarray analysis of RNA
US8076064B2 (en) 2005-07-09 2011-12-13 Agilent Technologies, Inc. Method of treatment of RNA sample
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US7700289B2 (en) 2006-04-03 2010-04-20 Agilent Technologies, Inc. Method for labeling RNA
US7572585B2 (en) 2006-07-31 2009-08-11 Agilent Technologies, Inc. Enzymatic labeling of RNA

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986000074A1 (en) * 1984-06-15 1986-01-03 Institut National De La Sante Et De La Recherche M Chemically marked nucleic acids, utilization thereof and kit for its implementation
WO1991017169A1 (en) * 1990-05-03 1991-11-14 Amersham International Plc Phosphoramidite derivatives, their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
WO1992002532A1 (en) * 1990-08-09 1992-02-20 Genta Incorporated Improved non-nucleotide-based linker reagents for oligomers
WO1992003464A1 (en) * 1990-08-28 1992-03-05 Microprobe Corporation Solid support synthesis of 3'-tailed oligonucleotides via a linking molecule

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5352578A (en) * 1989-02-15 1994-10-04 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
DE3916871A1 (en) * 1989-05-24 1990-11-29 Boehringer Mannheim Gmbh MODIFIED PHOSPHORAMIDITE PROCESS FOR THE PREPARATION OF MODIFIED NUCLEIC ACIDS
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE4123540A1 (en) * 1991-07-16 1993-01-21 Boehringer Mannheim Gmbh IMMOBILIZATION OF NUCLEIC ACIDS

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986000074A1 (en) * 1984-06-15 1986-01-03 Institut National De La Sante Et De La Recherche M Chemically marked nucleic acids, utilization thereof and kit for its implementation
WO1991017169A1 (en) * 1990-05-03 1991-11-14 Amersham International Plc Phosphoramidite derivatives, their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
WO1992002532A1 (en) * 1990-08-09 1992-02-20 Genta Incorporated Improved non-nucleotide-based linker reagents for oligomers
WO1992003464A1 (en) * 1990-08-28 1992-03-05 Microprobe Corporation Solid support synthesis of 3'-tailed oligonucleotides via a linking molecule

Also Published As

Publication number Publication date
WO1994012518A2 (en) 1994-06-09
DE4239531A1 (en) 1994-05-26

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