JPS57181692A - Method for removing cell wall of chlorella - Google Patents
Method for removing cell wall of chlorellaInfo
- Publication number
- JPS57181692A JPS57181692A JP56065449A JP6544981A JPS57181692A JP S57181692 A JPS57181692 A JP S57181692A JP 56065449 A JP56065449 A JP 56065449A JP 6544981 A JP6544981 A JP 6544981A JP S57181692 A JPS57181692 A JP S57181692A
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- pectinase
- mixture
- macerase
- protoplast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Seeds, Soups, And Other Foods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To remove the cell wall of chlorella, by converting the chlorella to protoplast or spheroplast using with a mixture of cellulase, macerase and pectinase or a mixture of cerulidine and pectinase.
CONSTITUTION: Chlorella cells of logarithmic growth stage are suspended in a weakly acidic buffer solution, mixed a mixture of cellulase, macerase and pectinase or a mixture of cerulidine and pectinase, and treated at 20W30°C for 2W24hr under light irradiation. The regenerable living protoplast or spheroplast of chlorella can be obtained by this process. The mixture of cellulase, macerase and pectinase is especially preferable for attaining high conversion to protoplast.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56065449A JPS57181692A (en) | 1981-04-30 | 1981-04-30 | Method for removing cell wall of chlorella |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56065449A JPS57181692A (en) | 1981-04-30 | 1981-04-30 | Method for removing cell wall of chlorella |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57181692A true JPS57181692A (en) | 1982-11-09 |
JPH0121954B2 JPH0121954B2 (en) | 1989-04-24 |
Family
ID=13287453
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56065449A Granted JPS57181692A (en) | 1981-04-30 | 1981-04-30 | Method for removing cell wall of chlorella |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57181692A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61141879A (en) * | 1984-12-17 | 1986-06-28 | Nisshin Oil Mills Ltd:The | Preparation of protoplast of chlorella |
JPS6232877A (en) * | 1985-08-06 | 1987-02-12 | Nisshin Oil Mills Ltd:The | Novel chlorella |
-
1981
- 1981-04-30 JP JP56065449A patent/JPS57181692A/en active Granted
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61141879A (en) * | 1984-12-17 | 1986-06-28 | Nisshin Oil Mills Ltd:The | Preparation of protoplast of chlorella |
JPH0126676B2 (en) * | 1984-12-17 | 1989-05-24 | Nisshin Oil Mills Ltd | |
JPS6232877A (en) * | 1985-08-06 | 1987-02-12 | Nisshin Oil Mills Ltd:The | Novel chlorella |
JPH0126675B2 (en) * | 1985-08-06 | 1989-05-24 | Nisshin Oil Mills Ltd |
Also Published As
Publication number | Publication date |
---|---|
JPH0121954B2 (en) | 1989-04-24 |
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