JPS57163500A - Determination of total cholesterol - Google Patents
Determination of total cholesterolInfo
- Publication number
- JPS57163500A JPS57163500A JP4627781A JP4627781A JPS57163500A JP S57163500 A JPS57163500 A JP S57163500A JP 4627781 A JP4627781 A JP 4627781A JP 4627781 A JP4627781 A JP 4627781A JP S57163500 A JPS57163500 A JP S57163500A
- Authority
- JP
- Japan
- Prior art keywords
- cholesterols
- type
- serum
- act
- hydrogen peroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: In a reaction system where cholesterol esterase and/or cholesterol oxidase are made to act on chelesterols in serum, a surface active agent with a single polyoxyethylene chain is used to effect the acceleration and activation of the reactions.
CONSTITUTION: Ester-type cholesterols in serum is hydrolyzed with cholesterol esterase into free-type cholesterols and free-type cholesterols are oxidized with eholesterol oxidase to form hydrogen peroxide, wherein a surfactant with a single polyoxyehtylene chain of the formula (R is 8W14C alkyl, n is 5W10 integer) is used to accelerate and activate the enzymatic reactions. The hydrogen peroxide formed is made to act on 4-aminoantipyrin in the presence of peroxidase to cause a coupling reaction with a couper of phenolic pigment and the colorimetry is effected. Any phenolic pigments may be used as a coupler.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4627781A JPS57163500A (en) | 1981-03-31 | 1981-03-31 | Determination of total cholesterol |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4627781A JPS57163500A (en) | 1981-03-31 | 1981-03-31 | Determination of total cholesterol |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57163500A true JPS57163500A (en) | 1982-10-07 |
JPH0146120B2 JPH0146120B2 (en) | 1989-10-05 |
Family
ID=12742724
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4627781A Granted JPS57163500A (en) | 1981-03-31 | 1981-03-31 | Determination of total cholesterol |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57163500A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62261962A (en) * | 1986-05-08 | 1987-11-14 | Chemo Sero Therapeut Res Inst | Liquid control serum for lipid component |
JPH07301636A (en) * | 1994-03-08 | 1995-11-14 | Kyowa Medex Co Ltd | Determination method for cholesterol in high density lipoprotein |
US6762062B2 (en) | 1999-01-27 | 2004-07-13 | Matsushita Electric Industrial Co., Ltd. | Method of determining cholesterol and sensor applicable to the same |
WO2005024052A1 (en) * | 2003-09-08 | 2005-03-17 | Kowa Co., Ltd. | Method of determining amount of cholesterol ester |
-
1981
- 1981-03-31 JP JP4627781A patent/JPS57163500A/en active Granted
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62261962A (en) * | 1986-05-08 | 1987-11-14 | Chemo Sero Therapeut Res Inst | Liquid control serum for lipid component |
JPH0588789B2 (en) * | 1986-05-08 | 1993-12-24 | Kagaku Oyobi Ketsusei Ryoho Kenkyusho | |
JPH07301636A (en) * | 1994-03-08 | 1995-11-14 | Kyowa Medex Co Ltd | Determination method for cholesterol in high density lipoprotein |
US6762062B2 (en) | 1999-01-27 | 2004-07-13 | Matsushita Electric Industrial Co., Ltd. | Method of determining cholesterol and sensor applicable to the same |
WO2005024052A1 (en) * | 2003-09-08 | 2005-03-17 | Kowa Co., Ltd. | Method of determining amount of cholesterol ester |
Also Published As
Publication number | Publication date |
---|---|
JPH0146120B2 (en) | 1989-10-05 |
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