JPS57150388A - Preparation of recombined dna - Google Patents

Preparation of recombined dna

Info

Publication number
JPS57150388A
JPS57150388A JP56034380A JP3438081A JPS57150388A JP S57150388 A JPS57150388 A JP S57150388A JP 56034380 A JP56034380 A JP 56034380A JP 3438081 A JP3438081 A JP 3438081A JP S57150388 A JPS57150388 A JP S57150388A
Authority
JP
Japan
Prior art keywords
dna
bacteriophage
restricted enzyme
recombined dna
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56034380A
Other languages
Japanese (ja)
Other versions
JPH028711B2 (en
Inventor
Takao Isogai
Hideo Takahashi
Akihisa Saitou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujisawa Pharmaceutical Co Ltd
Original Assignee
Fujisawa Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujisawa Pharmaceutical Co Ltd filed Critical Fujisawa Pharmaceutical Co Ltd
Priority to JP56034380A priority Critical patent/JPS57150388A/en
Publication of JPS57150388A publication Critical patent/JPS57150388A/en
Publication of JPH028711B2 publication Critical patent/JPH028711B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)

Abstract

PURPOSE:A recombined DNA is prepared by use of a DNA of a bacteriophage in a host of actinomycetes that has only one position cleavable with a restricted enzyme. CONSTITUTION:A restricted enzyme, Pvu II is made to act on the DNA of temperature bacteriophage in a host of actinomycetes to cleave it and the product is mixed with an extrinsic DNA having flash end. Then, a DNA-ligase is made to act thereon to give the objective recombined DNA. The DNA of temperate bacteriophage R4 is cleaved at only one position by restricted enzyme Pvu II and the position is different from that bearing genetic information.
JP56034380A 1981-03-09 1981-03-09 Preparation of recombined dna Granted JPS57150388A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56034380A JPS57150388A (en) 1981-03-09 1981-03-09 Preparation of recombined dna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56034380A JPS57150388A (en) 1981-03-09 1981-03-09 Preparation of recombined dna

Publications (2)

Publication Number Publication Date
JPS57150388A true JPS57150388A (en) 1982-09-17
JPH028711B2 JPH028711B2 (en) 1990-02-26

Family

ID=12412557

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56034380A Granted JPS57150388A (en) 1981-03-09 1981-03-09 Preparation of recombined dna

Country Status (1)

Country Link
JP (1) JPS57150388A (en)

Also Published As

Publication number Publication date
JPH028711B2 (en) 1990-02-26

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