JPS57136525A - Method of producing high-purity alpha-fetoprotein and immunoglobulin g - Google Patents

Method of producing high-purity alpha-fetoprotein and immunoglobulin g

Info

Publication number
JPS57136525A
JPS57136525A JP56021805A JP2180581A JPS57136525A JP S57136525 A JPS57136525 A JP S57136525A JP 56021805 A JP56021805 A JP 56021805A JP 2180581 A JP2180581 A JP 2180581A JP S57136525 A JPS57136525 A JP S57136525A
Authority
JP
Japan
Prior art keywords
immunoglobulin
purity
afp
combination
fetoprotein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56021805A
Other languages
Japanese (ja)
Inventor
Hiroo Horinishi
Susumu Sato
Tsutomu Honjo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morinaga and Co Ltd
Original Assignee
Morinaga and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morinaga and Co Ltd filed Critical Morinaga and Co Ltd
Priority to JP56021805A priority Critical patent/JPS57136525A/en
Publication of JPS57136525A publication Critical patent/JPS57136525A/en
Pending legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: A solution containing alpha-fetoprotein (AFP) is purified by a combination of specific treating methods to obtain ecomonically high-purity AFP used in the diagnosis of hepatic cancer, further the residual solution is subjected to salting out to give high-purity immunoglobulin G.
CONSTITUTION: A substance containing AFP such as placenta, blood, ascites or amniotic fluid or a solution obtained by partial purification of the above substance is used as a starting material and purified by a combination of acid precipitation, DEAE-cellulose column chromatography, hydroxyapatite chromatography or concanavalin chromatography. This purification is so effective that a product of higher than 99.9% purity is obtained from a starting material containing only 0.0025% AFP. Further, the residual solution and other substances containing immunoglobulin G are used and subjected to a combination of salting out and various kinds of chromatographies to give high-purity immunoglobulin G.
COPYRIGHT: (C)1982,JPO&Japio
JP56021805A 1981-02-17 1981-02-17 Method of producing high-purity alpha-fetoprotein and immunoglobulin g Pending JPS57136525A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56021805A JPS57136525A (en) 1981-02-17 1981-02-17 Method of producing high-purity alpha-fetoprotein and immunoglobulin g

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56021805A JPS57136525A (en) 1981-02-17 1981-02-17 Method of producing high-purity alpha-fetoprotein and immunoglobulin g

Publications (1)

Publication Number Publication Date
JPS57136525A true JPS57136525A (en) 1982-08-23

Family

ID=12065261

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56021805A Pending JPS57136525A (en) 1981-02-17 1981-02-17 Method of producing high-purity alpha-fetoprotein and immunoglobulin g

Country Status (1)

Country Link
JP (1) JPS57136525A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63146896A (en) * 1986-12-11 1988-06-18 Toa Nenryo Kogyo Kk Separation of antibody

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63146896A (en) * 1986-12-11 1988-06-18 Toa Nenryo Kogyo Kk Separation of antibody

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