JPS57132881A - Preparation of human hyaluronidase - Google Patents
Preparation of human hyaluronidaseInfo
- Publication number
- JPS57132881A JPS57132881A JP1867081A JP1867081A JPS57132881A JP S57132881 A JPS57132881 A JP S57132881A JP 1867081 A JP1867081 A JP 1867081A JP 1867081 A JP1867081 A JP 1867081A JP S57132881 A JPS57132881 A JP S57132881A
- Authority
- JP
- Japan
- Prior art keywords
- hyaluronidase
- urokinase
- human
- brought
- give
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To prepare human hyaluronidase of high purity without exhibiting any side effects on administering to the human body very efficiently, by treating a human urine with an adsorbent containing silicon.
CONSTITUTION: A human urine is brought into contact with an adsorbent containing silicon and the adsorbed substance is then eluted under alkaline conditions to give a raw material solution containing urokinase and hyaluronidase, which is then brought into contact with preferably a strongly basic anion exchanger to adsorb and remove the impurities from the raw material solution and give a concentrated aqueous solution containing the urokinase and the hyaluronidase. The urokinase is then obtained from the concentrated aqueous solution by the conventional method, and the hyaluronidase is collected from the waste fraction after obtaining the urokinase, by fractionating with ammonium sulfate, a zinc salt and acetone.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1867081A JPS57132881A (en) | 1981-02-10 | 1981-02-10 | Preparation of human hyaluronidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1867081A JPS57132881A (en) | 1981-02-10 | 1981-02-10 | Preparation of human hyaluronidase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57132881A true JPS57132881A (en) | 1982-08-17 |
Family
ID=11978030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1867081A Pending JPS57132881A (en) | 1981-02-10 | 1981-02-10 | Preparation of human hyaluronidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57132881A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999029841A1 (en) * | 1997-12-09 | 1999-06-17 | The Regents Of The University Of California | Purification and microsequencing of hyaluronidase isozymes |
-
1981
- 1981-02-10 JP JP1867081A patent/JPS57132881A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6123938A (en) * | 1996-10-17 | 2000-09-26 | The Regents Of The University Of California | Human urinary hyaluronidase |
WO1999029841A1 (en) * | 1997-12-09 | 1999-06-17 | The Regents Of The University Of California | Purification and microsequencing of hyaluronidase isozymes |
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