JPS57106853A - Enzyme electrode - Google Patents

Enzyme electrode

Info

Publication number
JPS57106853A
JPS57106853A JP55181844A JP18184480A JPS57106853A JP S57106853 A JPS57106853 A JP S57106853A JP 55181844 A JP55181844 A JP 55181844A JP 18184480 A JP18184480 A JP 18184480A JP S57106853 A JPS57106853 A JP S57106853A
Authority
JP
Japan
Prior art keywords
membrane
enzyme
concentration
electrode
cathode
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55181844A
Other languages
Japanese (ja)
Inventor
Masao Koyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toshiba Corp
Original Assignee
Toshiba Corp
Tokyo Shibaura Electric Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Corp, Tokyo Shibaura Electric Co Ltd filed Critical Toshiba Corp
Priority to JP55181844A priority Critical patent/JPS57106853A/en
Publication of JPS57106853A publication Critical patent/JPS57106853A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To improve the sensitivity, by making higher the concentration of enzyme content in the crosssectional direction of an immobilized enzyme carrier layer on the side of an electrode and making lower said concentration on the side of a sample liquid. CONSTITUTION:An oxygen electrode 1 as the base electrode is constituted by an anode 2 made of Ag/silver chloride, a cathode 3 made of platinum, a saturated KCl electrolyte 4 filled up between these electrodes, an insulating cylindrical body 5 accommodating said electrodes 2 and 3 and an oxygen permeable teflon membrane 6 provided closely stuck on the lower tip faces of the cathode 3 and the body 5. An immobilized glucose oxidase membrane 7 is attached on the outside of the membrane 6. The thickness of the membrane 7 is 25mum and about 1mg/cm<2> of glucose oxidase is fixed on an inner layer 7a of the inside 2/3 and enzyme is not contained nearly in the outer layer 7b of the outside 1/3. By distributing the concentration of enzyme, the sensitivity of detection is improved and a highly accurate analysis is made.
JP55181844A 1980-12-24 1980-12-24 Enzyme electrode Pending JPS57106853A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55181844A JPS57106853A (en) 1980-12-24 1980-12-24 Enzyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55181844A JPS57106853A (en) 1980-12-24 1980-12-24 Enzyme electrode

Publications (1)

Publication Number Publication Date
JPS57106853A true JPS57106853A (en) 1982-07-02

Family

ID=16107798

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55181844A Pending JPS57106853A (en) 1980-12-24 1980-12-24 Enzyme electrode

Country Status (1)

Country Link
JP (1) JPS57106853A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014503080A (en) * 2011-01-20 2014-02-06 メドトロニック ミニメド インコーポレイテッド Layered enzyme composition for use with an analyte sensor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014503080A (en) * 2011-01-20 2014-02-06 メドトロニック ミニメド インコーポレイテッド Layered enzyme composition for use with an analyte sensor

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