JPS57102195A - Measuring method of superoxide dismutase - Google Patents

Measuring method of superoxide dismutase

Info

Publication number
JPS57102195A
JPS57102195A JP18093880A JP18093880A JPS57102195A JP S57102195 A JPS57102195 A JP S57102195A JP 18093880 A JP18093880 A JP 18093880A JP 18093880 A JP18093880 A JP 18093880A JP S57102195 A JPS57102195 A JP S57102195A
Authority
JP
Japan
Prior art keywords
superoxide dismutase
solution containing
solution
titled substance
receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP18093880A
Other languages
Japanese (ja)
Other versions
JPH0231349B2 (en
Inventor
Minoru Sakakibara
Masatoshi Tsujino
Nobuaki Nakagawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Jozo KK
Original Assignee
Toyo Jozo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Jozo KK filed Critical Toyo Jozo KK
Priority to JP18093880A priority Critical patent/JPH0231349B2/en
Priority to FR8123500A priority patent/FR2496692B1/en
Priority to GB8138258A priority patent/GB2089980B/en
Publication of JPS57102195A publication Critical patent/JPS57102195A/en
Publication of JPH0231349B2 publication Critical patent/JPH0231349B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Abstract

PURPOSE: To measure the titled substance easily and well, by reacting a solution containing a combination of the titled substance with a labeling enzyme with a test solution and a solution containing a receptor having the ability to combine with the titled substance in an aqueous medium, and measuring the enzymic activity.
CONSTITUTION: A superoxide dismutase is combined with an enzyme, e.g. lipase, alkaline phosphatase or glycerol kinase, and a given amount of the resultant solution containing the combination thereof is reacted with a test solution for determining the superoxide dismutase value, a receptor having a specific bonding property to the superoxide dismutase, e.g. an antibody obtained by sensitizing a different kind of animal with the superoxide dismutase or a solution containing the an antiserum containing the antibody, at about 4°C overnight. The reaction is carried out in an aqueous medium, e.g. a buffer solution. After the reaction, the solid phase is separated from the liquid layer, and the enzymic activity of the solid phase or liquid layer is measured.
COPYRIGHT: (C)1982,JPO&Japio
JP18093880A 1980-12-19 1980-12-19 HITOMATAHAUSHURAINOSUUPAAOKISHIDO * DESUMUTAAZENOSOKUTEIHO Expired - Lifetime JPH0231349B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP18093880A JPH0231349B2 (en) 1980-12-19 1980-12-19 HITOMATAHAUSHURAINOSUUPAAOKISHIDO * DESUMUTAAZENOSOKUTEIHO
FR8123500A FR2496692B1 (en) 1980-12-19 1981-12-16 PEROXIDE DISMUTASE ASSAY PROCESS
GB8138258A GB2089980B (en) 1980-12-19 1981-12-18 Method for assaying superoxide dismutase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18093880A JPH0231349B2 (en) 1980-12-19 1980-12-19 HITOMATAHAUSHURAINOSUUPAAOKISHIDO * DESUMUTAAZENOSOKUTEIHO

Publications (2)

Publication Number Publication Date
JPS57102195A true JPS57102195A (en) 1982-06-25
JPH0231349B2 JPH0231349B2 (en) 1990-07-12

Family

ID=16091896

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18093880A Expired - Lifetime JPH0231349B2 (en) 1980-12-19 1980-12-19 HITOMATAHAUSHURAINOSUUPAAOKISHIDO * DESUMUTAAZENOSOKUTEIHO

Country Status (3)

Country Link
JP (1) JPH0231349B2 (en)
FR (1) FR2496692B1 (en)
GB (1) GB2089980B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60109528A (en) * 1983-10-04 1985-06-15 Fujisawa Pharmaceut Co Ltd Antisuperoxide dismutase monoclonal antibody and hybridoma producing the same
JPS61212600A (en) * 1985-03-19 1986-09-20 Ube Ind Ltd Monoclonal antibody to human copper, zinc superoxide dismutase and production thereof
JPH05184359A (en) * 1983-10-04 1993-07-27 Fujisawa Pharmaceut Co Ltd Anti-superoxide dismutase monoclonal antibody and hybridoma producing the antibody

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1272943A (en) * 1985-03-01 1990-08-21 Toshiro Hanada Process for determining superoxide dismutase activity
US4940659A (en) * 1987-02-20 1990-07-10 Monoclonetics International, Inc. Screening extra-cellular body fluids for superoxide dismutase (SOD-1) for determining fetal trisomy 21 down syndrome
DE4335057A1 (en) * 1993-10-11 1995-04-13 Seramun Diagnostica Gmbh Method and test kit for detecting cytotoxic mechanisms in vitro

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60109528A (en) * 1983-10-04 1985-06-15 Fujisawa Pharmaceut Co Ltd Antisuperoxide dismutase monoclonal antibody and hybridoma producing the same
JPH05184359A (en) * 1983-10-04 1993-07-27 Fujisawa Pharmaceut Co Ltd Anti-superoxide dismutase monoclonal antibody and hybridoma producing the antibody
JPS61212600A (en) * 1985-03-19 1986-09-20 Ube Ind Ltd Monoclonal antibody to human copper, zinc superoxide dismutase and production thereof

Also Published As

Publication number Publication date
GB2089980B (en) 1984-07-18
GB2089980A (en) 1982-06-30
JPH0231349B2 (en) 1990-07-12
FR2496692A1 (en) 1982-06-25
FR2496692B1 (en) 1985-06-21

Similar Documents

Publication Publication Date Title
JPS5592693A (en) Determination of ligand
US3873269A (en) Indicator for the determination of urea
Self Enzyme amplification—a general method applied to provide an immunoassisted assay for placental alkaline phosphatase
EP0093613B1 (en) Simultaneous calibration heterogeneous immunoassay method, apparatus and diagnostic kit
ATE14630T1 (en) CREATININE ANTIBODIES.
ATE30780T1 (en) PROCEDURE FOR DETERMINING BIOACTIVE SUBSTANCES.
JPS57136165A (en) Immunological measuring reagent
JPS57144996A (en) Film for quantitative analysis
Chen et al. Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin.
JPS57102195A (en) Measuring method of superoxide dismutase
EP0113075A3 (en) Field immunoassay reaction system, method of immunoassay diagnosis and probe or carrier for use therewith
Litman et al. An internally referenced test strip immunoassay for morphine.
ES8507265A1 (en) Method of measuring biological ligands.
JPS5512419A (en) Immunological measurement and reagent therefor
JPS57102196A (en) Measuring method of superoxide dismutase (sod)
US5817454A (en) Portable apparatus and method for detection of methylxanthine chemical species
US3493467A (en) Reversible biochemical reaction employing a trapping agent
JPS5714748A (en) Kit for quantitative determination of valproic acid and its method for quantitative determination
JPS5629168A (en) Measuring method of body fluid component by immunity chemical reaction
JPS5697863A (en) Method and apparatus for analyzing amylase
JPS56158098A (en) Measurement of alpha-amylase activity
JPS6463864A (en) Immunoassay
JPS5535247A (en) Reagent for h-lysozyme measurement
FI88310C (en) FOERFARANDE OCH MEDEL FOER BESTAEMNING AV UREA
JPS56156220A (en) Insoluble antigen or antibody, its preparation and measuring method of antigen and antibody with the same