JPS5696693A - Production of human standard enzyme sample from human placenta - Google Patents
Production of human standard enzyme sample from human placentaInfo
- Publication number
- JPS5696693A JPS5696693A JP13448680A JP13448680A JPS5696693A JP S5696693 A JPS5696693 A JP S5696693A JP 13448680 A JP13448680 A JP 13448680A JP 13448680 A JP13448680 A JP 13448680A JP S5696693 A JPS5696693 A JP S5696693A
- Authority
- JP
- Japan
- Prior art keywords
- human
- enzyme
- combined
- solution
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: An enzyme mix extracted from readily available human placentas is combined with coenzymes, enzyme activity stabilizer and a nonionic surfactant to form an enzyme solution, which is freeze dried to produce a human standard enzyme sample with high stability.
CONSTITUTION: The washings of human placentas with physiological salt solution is centrigfuged to collect erythrocytes. The resultant erthrocytes are combined with the same volume of distilled water to form an erythrocyte suspension. A 3:1 mixture of ethanol and chloroform is added to the suspension at a ratio of 0.44 part to 1 part of the erythrocyte suspension to extract almost all the enzymes included in etythrocytes such as catalase. Then, the extract is combined with a solution containing NADH2 (reduction type of β-nicotinic amide adenine-2-nucleotide), an enzyme activity stabilizer such as polyvinyl pyrrolidone and a nonionic surfactant such as Triton X-100, e.g., a phosphoric buffer solution and they are thoroughly mixed and freeze dried.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13448680A JPS5696693A (en) | 1980-09-29 | 1980-09-29 | Production of human standard enzyme sample from human placenta |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13448680A JPS5696693A (en) | 1980-09-29 | 1980-09-29 | Production of human standard enzyme sample from human placenta |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6280876A Division JPS52145290A (en) | 1976-05-28 | 1976-05-28 | Preparation process for standard enzyme specimen of human from placenta |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5696693A true JPS5696693A (en) | 1981-08-04 |
JPS5651748B2 JPS5651748B2 (en) | 1981-12-08 |
Family
ID=15129448
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13448680A Granted JPS5696693A (en) | 1980-09-29 | 1980-09-29 | Production of human standard enzyme sample from human placenta |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5696693A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02276738A (en) * | 1989-04-17 | 1990-11-13 | Omron Corp | Continuously driving-out device of paper |
-
1980
- 1980-09-29 JP JP13448680A patent/JPS5696693A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5651748B2 (en) | 1981-12-08 |
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