JPS5642588A - Production of elastase - Google Patents
Production of elastaseInfo
- Publication number
- JPS5642588A JPS5642588A JP11712179A JP11712179A JPS5642588A JP S5642588 A JPS5642588 A JP S5642588A JP 11712179 A JP11712179 A JP 11712179A JP 11712179 A JP11712179 A JP 11712179A JP S5642588 A JPS5642588 A JP S5642588A
- Authority
- JP
- Japan
- Prior art keywords
- elastase
- precipitate
- salt
- mammarian
- autolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: A pancreas of mammarian is subjected to autolysis, filtered, and the filtrate is treated with a salt of alkaline earth metal to produce elastase complex or pure elastase.
CONSTITUTION: A pancrease of mammarian, preferably a pancreas of pig, is subjected to autolysis at 40W50°C for at least 1hr and the filtrate is combined with a salt of alkaline earth metal such as calcium chloride or magnesium chloride in the presence of a water-soluble organic solvent to form a precipitate. The precipitate is removed and the extract of elastase is obtained. A salt, preferably ammonium sulfate, is added to the extract to precipitate elastase. The elastase is dissolved in an aqueous basic solution, desalted, and neutralized to precipitate elastase complex as crystals. Or the desalted solution is passed through a weakly basic anion exchanger to collect the unadsorbed fraction. Then, the unadsorbed fraction is neutralized to precipitate pure elastase as crystals.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11712179A JPS5642588A (en) | 1979-09-11 | 1979-09-11 | Production of elastase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11712179A JPS5642588A (en) | 1979-09-11 | 1979-09-11 | Production of elastase |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP23164584A Division JPS6054037B2 (en) | 1984-11-02 | 1984-11-02 | Manufacturing method of elastase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5642588A true JPS5642588A (en) | 1981-04-20 |
JPS579356B2 JPS579356B2 (en) | 1982-02-20 |
Family
ID=14703941
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11712179A Granted JPS5642588A (en) | 1979-09-11 | 1979-09-11 | Production of elastase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5642588A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63133361U (en) * | 1987-02-25 | 1988-08-31 |
-
1979
- 1979-09-11 JP JP11712179A patent/JPS5642588A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS579356B2 (en) | 1982-02-20 |
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