JPS5642588A - Production of elastase - Google Patents

Production of elastase

Info

Publication number
JPS5642588A
JPS5642588A JP11712179A JP11712179A JPS5642588A JP S5642588 A JPS5642588 A JP S5642588A JP 11712179 A JP11712179 A JP 11712179A JP 11712179 A JP11712179 A JP 11712179A JP S5642588 A JPS5642588 A JP S5642588A
Authority
JP
Japan
Prior art keywords
elastase
precipitate
salt
mammarian
autolysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11712179A
Other languages
Japanese (ja)
Other versions
JPS579356B2 (en
Inventor
Tsuneo Miyake
Sanji Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OKURA KASEI KK
OOKURA KASEI KK
Original Assignee
OKURA KASEI KK
OOKURA KASEI KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OKURA KASEI KK, OOKURA KASEI KK filed Critical OKURA KASEI KK
Priority to JP11712179A priority Critical patent/JPS5642588A/en
Publication of JPS5642588A publication Critical patent/JPS5642588A/en
Publication of JPS579356B2 publication Critical patent/JPS579356B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: A pancreas of mammarian is subjected to autolysis, filtered, and the filtrate is treated with a salt of alkaline earth metal to produce elastase complex or pure elastase.
CONSTITUTION: A pancrease of mammarian, preferably a pancreas of pig, is subjected to autolysis at 40W50°C for at least 1hr and the filtrate is combined with a salt of alkaline earth metal such as calcium chloride or magnesium chloride in the presence of a water-soluble organic solvent to form a precipitate. The precipitate is removed and the extract of elastase is obtained. A salt, preferably ammonium sulfate, is added to the extract to precipitate elastase. The elastase is dissolved in an aqueous basic solution, desalted, and neutralized to precipitate elastase complex as crystals. Or the desalted solution is passed through a weakly basic anion exchanger to collect the unadsorbed fraction. Then, the unadsorbed fraction is neutralized to precipitate pure elastase as crystals.
COPYRIGHT: (C)1981,JPO&Japio
JP11712179A 1979-09-11 1979-09-11 Production of elastase Granted JPS5642588A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11712179A JPS5642588A (en) 1979-09-11 1979-09-11 Production of elastase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11712179A JPS5642588A (en) 1979-09-11 1979-09-11 Production of elastase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP23164584A Division JPS6054037B2 (en) 1984-11-02 1984-11-02 Manufacturing method of elastase

Publications (2)

Publication Number Publication Date
JPS5642588A true JPS5642588A (en) 1981-04-20
JPS579356B2 JPS579356B2 (en) 1982-02-20

Family

ID=14703941

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11712179A Granted JPS5642588A (en) 1979-09-11 1979-09-11 Production of elastase

Country Status (1)

Country Link
JP (1) JPS5642588A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63133361U (en) * 1987-02-25 1988-08-31

Also Published As

Publication number Publication date
JPS579356B2 (en) 1982-02-20

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