JPS5632992A - Preparation of ribonuclease t1 - Google Patents

Preparation of ribonuclease t1

Info

Publication number
JPS5632992A
JPS5632992A JP10771079A JP10771079A JPS5632992A JP S5632992 A JPS5632992 A JP S5632992A JP 10771079 A JP10771079 A JP 10771079A JP 10771079 A JP10771079 A JP 10771079A JP S5632992 A JPS5632992 A JP S5632992A
Authority
JP
Japan
Prior art keywords
ribonuclease
chromatography
culture medium
mixture obtained
aspergillus oryzae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10771079A
Other languages
Japanese (ja)
Other versions
JPS6347440B2 (en
Inventor
Isao Uchida
Shigenori Kanetani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP10771079A priority Critical patent/JPS5632992A/en
Publication of JPS5632992A publication Critical patent/JPS5632992A/en
Publication of JPS6347440B2 publication Critical patent/JPS6347440B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: An enzyme mixture obtained from the culture medium for Aspergillus oryzae is subjected to a chromatography using a specific affinity adsorbent to produce ribonuclease T1 in hihg yield.
CONSTITUTION: An enzyme mixture obtained from the culture medium of Aspergillus oryzae, that is' an aqueous solution of takadiastase mainly containing diastase, is purified by subjecting it to a chromatography using an affinity adsorbent of the formula in which 5'-guanosine monophosphate is the ligand, which links with the 2'-C in the ribose at the position of the 3'-C, to give the ribonuclease T1.
COPYRIGHT: (C)1981,JPO&Japio
JP10771079A 1979-08-24 1979-08-24 Preparation of ribonuclease t1 Granted JPS5632992A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10771079A JPS5632992A (en) 1979-08-24 1979-08-24 Preparation of ribonuclease t1

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10771079A JPS5632992A (en) 1979-08-24 1979-08-24 Preparation of ribonuclease t1

Publications (2)

Publication Number Publication Date
JPS5632992A true JPS5632992A (en) 1981-04-02
JPS6347440B2 JPS6347440B2 (en) 1988-09-21

Family

ID=14465978

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10771079A Granted JPS5632992A (en) 1979-08-24 1979-08-24 Preparation of ribonuclease t1

Country Status (1)

Country Link
JP (1) JPS5632992A (en)

Also Published As

Publication number Publication date
JPS6347440B2 (en) 1988-09-21

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