JPS5629992A - Method of freezing hepatocyte - Google Patents

Method of freezing hepatocyte

Info

Publication number
JPS5629992A
JPS5629992A JP10356579A JP10356579A JPS5629992A JP S5629992 A JPS5629992 A JP S5629992A JP 10356579 A JP10356579 A JP 10356579A JP 10356579 A JP10356579 A JP 10356579A JP S5629992 A JPS5629992 A JP S5629992A
Authority
JP
Japan
Prior art keywords
protecting
extracellularly
intracellularly
suspension
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10356579A
Other languages
Japanese (ja)
Other versions
JPS6029471B2 (en
Inventor
Jiro Kawarabata
Tatsuo Suzuki
Masashi Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nikkiso Co Ltd
Original Assignee
Nikkiso Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nikkiso Co Ltd filed Critical Nikkiso Co Ltd
Priority to JP54103565A priority Critical patent/JPS6029471B2/en
Publication of JPS5629992A publication Critical patent/JPS5629992A/en
Publication of JPS6029471B2 publication Critical patent/JPS6029471B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

PURPOSE: Substance intracellularly and extracellularly protecting cells from freeze damage are added to a hepatocyte suspension and the suspension is frozen by gradual cooling, whereby higher than 80% viability of hepatocytes are ensured.
CONSTITUTION: To a suspension of hepatocytes are added at least one of substances intracellularly protecing from freeze damage selected from glycerol and dimethyl sulfoxide and at least one of substances extracellularly protecting from freeze damage selected from dextrin and albumin wherein the concentration of the intracellularly protecting substance is adjusted to 20w/v% or lower as glycerol or to 15w/v% or lower as dimethyl sulfoxide and in the range from 2W20w/v% as the total, while the extracellularly protecting substance is adjusted to 20w/v% or lower and the cooling rate is controlled to 0.1W3°C/min.
COPYRIGHT: (C)1981,JPO&Japio
JP54103565A 1979-08-16 1979-08-16 How to freeze liver cells Expired JPS6029471B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP54103565A JPS6029471B2 (en) 1979-08-16 1979-08-16 How to freeze liver cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP54103565A JPS6029471B2 (en) 1979-08-16 1979-08-16 How to freeze liver cells

Publications (2)

Publication Number Publication Date
JPS5629992A true JPS5629992A (en) 1981-03-25
JPS6029471B2 JPS6029471B2 (en) 1985-07-10

Family

ID=14357320

Family Applications (1)

Application Number Title Priority Date Filing Date
JP54103565A Expired JPS6029471B2 (en) 1979-08-16 1979-08-16 How to freeze liver cells

Country Status (1)

Country Link
JP (1) JPS6029471B2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02500800A (en) * 1987-06-23 1990-03-22 イスティトゥト　ナツィオナーレ　ペル　ラリセルカ　スル　カンクロ How to preserve epithelial tissue explants cultured in vitro
JP2722134B2 (en) * 1990-06-04 1998-03-04 バイオサーフェイス テクノロジー インコーポレイテッド Cryopreservation of cultured epithelial cell sheets
WO2003085100A1 (en) * 2002-04-04 2003-10-16 Daiichi Pure Chemicals Co., Ltd. Method of culturing liver cells over long time
JP2007119372A (en) * 2005-10-26 2007-05-17 Hiroshima Univ Method for freezing and storing extracted tooth

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4808819B2 (en) * 2009-08-21 2011-11-02 新日本製鐵株式会社 Non-fired carbon-containing agglomerated mineral for blast furnace and method for producing the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02500800A (en) * 1987-06-23 1990-03-22 イスティトゥト　ナツィオナーレ　ペル　ラリセルカ　スル　カンクロ How to preserve epithelial tissue explants cultured in vitro
JP2722134B2 (en) * 1990-06-04 1998-03-04 バイオサーフェイス テクノロジー インコーポレイテッド Cryopreservation of cultured epithelial cell sheets
WO2003085100A1 (en) * 2002-04-04 2003-10-16 Daiichi Pure Chemicals Co., Ltd. Method of culturing liver cells over long time
CN1303205C (en) * 2002-04-04 2007-03-07 第一化学药品株式会社 Method of culturing liver cells over long time
JP2007119372A (en) * 2005-10-26 2007-05-17 Hiroshima Univ Method for freezing and storing extracted tooth

Also Published As

Publication number Publication date
JPS6029471B2 (en) 1985-07-10

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