JPS56157838A - Living body tissue sample for electron microscope and method and apparatus for preparing the same - Google Patents

Living body tissue sample for electron microscope and method and apparatus for preparing the same

Info

Publication number
JPS56157838A
JPS56157838A JP6054180A JP6054180A JPS56157838A JP S56157838 A JPS56157838 A JP S56157838A JP 6054180 A JP6054180 A JP 6054180A JP 6054180 A JP6054180 A JP 6054180A JP S56157838 A JPS56157838 A JP S56157838A
Authority
JP
Japan
Prior art keywords
slices
foil plate
metal foil
metal
fitted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP6054180A
Other languages
Japanese (ja)
Inventor
Yukitaka Ajimi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP6054180A priority Critical patent/JPS56157838A/en
Publication of JPS56157838A publication Critical patent/JPS56157838A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To enable sample slices to be cut with the temperature thereof maintained accurately, by a method wherein a metal foil plate supporting sample slices fitted in slits in instantaneously pressure-bonded by two metal blocks cooled to ultralow temperature to form a glasslike frozen layer having no formation of cryohydrate on the surface. CONSTITUTION:Living body slices 1 are fitted in a plurality of slits in a metal foil plate 3 of excellent heat conductivity. After metal blocks 7 and 8 have been cooled in a cooling chamber 4, an electromagnet 10 is operated to raise the metal block 8, and a supporting base 12 is fitted. The metal foil plate 3 is placed on a supporting member 20, which is attached to the lower end of a cylinder 6 by using a rubber band 21, and a lid-shaped body 5 is lowered to cover the cooling chamber 4. A rod-shaped weight 11 is inserted into the cylinder 6, and the metal block 8 and the metal foil plate 3 are made to drop. Consequently, the slices 1 are pressure-bonded from both sides by means of the cooled metal blocks 7 and 8 and instantaneously frozen to form a glasslike frozen layer 2 having no formation of cryohydrate. The slices 1 are made to bite into the metal foil plate 3 and firmly fixed, each being formed into an I-shape. Accordingly, controlling the temperature of the metal foil plate 3 permits that of the slices to be controlled.
JP6054180A 1980-05-09 1980-05-09 Living body tissue sample for electron microscope and method and apparatus for preparing the same Pending JPS56157838A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6054180A JPS56157838A (en) 1980-05-09 1980-05-09 Living body tissue sample for electron microscope and method and apparatus for preparing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6054180A JPS56157838A (en) 1980-05-09 1980-05-09 Living body tissue sample for electron microscope and method and apparatus for preparing the same

Publications (1)

Publication Number Publication Date
JPS56157838A true JPS56157838A (en) 1981-12-05

Family

ID=13145249

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6054180A Pending JPS56157838A (en) 1980-05-09 1980-05-09 Living body tissue sample for electron microscope and method and apparatus for preparing the same

Country Status (1)

Country Link
JP (1) JPS56157838A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5912040U (en) * 1982-07-13 1984-01-25 株式会社エイコ−エンジニアリング Sample rapid freezing device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5127980A (en) * 1974-09-02 1976-03-09 Toa Electric Co Ltd
JPS51149769A (en) * 1975-05-19 1976-12-22 Hitachi Ltd Freeze hracture replication method and equipment
JPS559401B1 (en) * 1970-11-24 1980-03-10

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS559401B1 (en) * 1970-11-24 1980-03-10
JPS5127980A (en) * 1974-09-02 1976-03-09 Toa Electric Co Ltd
JPS51149769A (en) * 1975-05-19 1976-12-22 Hitachi Ltd Freeze hracture replication method and equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5912040U (en) * 1982-07-13 1984-01-25 株式会社エイコ−エンジニアリング Sample rapid freezing device
JPH0110602Y2 (en) * 1982-07-13 1989-03-27

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