JPS5574794A - Preparation of immobilized uricase membrane - Google Patents
Preparation of immobilized uricase membraneInfo
- Publication number
- JPS5574794A JPS5574794A JP14739178A JP14739178A JPS5574794A JP S5574794 A JPS5574794 A JP S5574794A JP 14739178 A JP14739178 A JP 14739178A JP 14739178 A JP14739178 A JP 14739178A JP S5574794 A JPS5574794 A JP S5574794A
- Authority
- JP
- Japan
- Prior art keywords
- uricase
- membrane
- preparation
- chitosan
- immersing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To prepare a uricase membrane having excellent dimensional stability, mechanical strength, and storage stability, with little deactivation of uricase, by casting an acidic solution of chitosan and uricase, and immersing the dried film thus prepared in an alkaline solution.
CONSTITUTION: Uricase is dissolved in an acidic aqueous solution of chitosan having a concentration of about 0.5W2% and a pH value of about 1W6, and cast to a horizontal plate to obtain a membrane having a thickness of about 10W100μ. The membrane is dried to a water-content of ≤ about 15%, and coagulated by immersing in an alkaline solution having a pH of 9W13 for about 10W60 minutes.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147391A JPS5837836B2 (en) | 1978-11-28 | 1978-11-28 | Method for producing immobilized uricase membrane |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53147391A JPS5837836B2 (en) | 1978-11-28 | 1978-11-28 | Method for producing immobilized uricase membrane |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5574794A true JPS5574794A (en) | 1980-06-05 |
JPS5837836B2 JPS5837836B2 (en) | 1983-08-18 |
Family
ID=15429195
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53147391A Expired JPS5837836B2 (en) | 1978-11-28 | 1978-11-28 | Method for producing immobilized uricase membrane |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5837836B2 (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52130980A (en) * | 1976-04-21 | 1977-11-02 | Toyobo Co Ltd | Immobilization of glucoseisomerase |
JPS5332190A (en) * | 1976-09-03 | 1978-03-27 | Toyobo Co Ltd | Immobilization of glucoseisomerase |
-
1978
- 1978-11-28 JP JP53147391A patent/JPS5837836B2/en not_active Expired
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52130980A (en) * | 1976-04-21 | 1977-11-02 | Toyobo Co Ltd | Immobilization of glucoseisomerase |
JPS5332190A (en) * | 1976-09-03 | 1978-03-27 | Toyobo Co Ltd | Immobilization of glucoseisomerase |
Also Published As
Publication number | Publication date |
---|---|
JPS5837836B2 (en) | 1983-08-18 |
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