JPS5551357A - Arbovirus agglutination method - Google Patents
Arbovirus agglutination methodInfo
- Publication number
- JPS5551357A JPS5551357A JP12543478A JP12543478A JPS5551357A JP S5551357 A JPS5551357 A JP S5551357A JP 12543478 A JP12543478 A JP 12543478A JP 12543478 A JP12543478 A JP 12543478A JP S5551357 A JPS5551357 A JP S5551357A
- Authority
- JP
- Japan
- Prior art keywords
- red blood
- blood cell
- arbovirus
- buffer solution
- agglutination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To enable the test over a wide range of pH by carrying out the red blood cell agglutination (HA) or anti-agglutination (HI) for the arbovirus by means of the fixed bird red blood cell.
CONSTITUTION: The bird red blood cell (the chicken of within 48 hours after hatching and with no food given is used preferably) is processed via the fixing agent such as formalin, glutamyl aldehyde or the like. The above mentioned fixed red blood cell and the arbovirus special HA antigen are diluted by the buffer solution of pH6W12 to secure the HA reaction. At the same time, the above mentioned fixed red blood cell, the arbovirus special HA antigen and the blood serum to be tested are diluted by the buffer solution of pH6.6W12 to secure the HI reaction. In this case, the phosphorus buffer brine, HEPES buffer solution and the like are used for the buffer solution, and about 0.1W1% of the blood serum albumin and about 0.05W0.00025% of gelatin are added preferably.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12543478A JPS5551357A (en) | 1978-10-11 | 1978-10-11 | Arbovirus agglutination method |
| PCT/JP1979/000010 WO1979000675A1 (en) | 1978-02-06 | 1979-01-17 | Improvement in viral hemoagglutination inhibition test |
| DE19792938820 DE2938820A1 (en) | 1978-02-06 | 1979-01-17 | IMPROVEMENT IN VIRAL HEMOAGGLUTINATION INHIBITION TEST |
| GB7934191A GB2036309B (en) | 1978-02-06 | 1979-01-17 | Viral hemoagglutination inhibition test |
| US06/085,641 US4298346A (en) | 1978-02-06 | 1979-01-29 | Virus hemagglutination-inhibition reaction |
| FR7902946A FR2416471A1 (en) | 1978-02-06 | 1979-02-05 | IMPROVEMENT FOR THE REACTION OF INHIBITION OF HEMO-AGGLUTINATION OF VIRUSES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12543478A JPS5551357A (en) | 1978-10-11 | 1978-10-11 | Arbovirus agglutination method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5551357A true JPS5551357A (en) | 1980-04-15 |
Family
ID=14909987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12543478A Pending JPS5551357A (en) | 1978-02-06 | 1978-10-11 | Arbovirus agglutination method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5551357A (en) |
-
1978
- 1978-10-11 JP JP12543478A patent/JPS5551357A/en active Pending
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