JPS5550895A - Extract of bacterial cells, its preparation, and anti- tumor agent containing the same - Google Patents

Extract of bacterial cells, its preparation, and anti- tumor agent containing the same

Info

Publication number
JPS5550895A
JPS5550895A JP12435978A JP12435978A JPS5550895A JP S5550895 A JPS5550895 A JP S5550895A JP 12435978 A JP12435978 A JP 12435978A JP 12435978 A JP12435978 A JP 12435978A JP S5550895 A JPS5550895 A JP S5550895A
Authority
JP
Japan
Prior art keywords
extract
cells
removing treatment
bacterial cells
delayed allergy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12435978A
Other languages
Japanese (ja)
Other versions
JPS6326117B2 (en
Inventor
Tadashi Sudo
Tamotsu Fukuda
Osamu Yano
Hitoshi Inoue
Hayao Abe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Priority to JP12435978A priority Critical patent/JPS5550895A/en
Publication of JPS5550895A publication Critical patent/JPS5550895A/en
Publication of JPS6326117B2 publication Critical patent/JPS6326117B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

PURPOSE: To prepare a novel extract of bacterial cells, having anti-tumor activity, by disintegrating the cells of facteria having delayed allergy activating capability, extracting the disintegrated cells, and purifying the extract.
CONSTITUTION: An extract of bacterial cells, useful as an anti-tumor agent, containing ≤3% of lipids, ≤1% of nucleic acids, and ≤65% of glucides, and having a delayed allergy activating capability per unit weight of the protein of more than 4 times as high as the purified tuberculin, is prepared by the following steps; (1) mixing the cells of bacteria having delayed allergy activating capability, e.g. Mycobacterium tuberculosis, Mycobacterium smegmatis, etc. with a proper buffer solution, (2) disintegrating the cells with a disintegrator, (3) filtering or centrifuging the dispersion of the disintegrated cells to obtain an extract, (4) subjecting the extract to the nucleic acid-removing treatment and low-activity protein-removing treatment, (5) if necessary, subjecting the extract further to the lipid-removing treatment and/ or the glucide-removing treatment, and finally (6) fractionating the purified product with an ion exchange meterial.
COPYRIGHT: (C)1980,JPO&Japio
JP12435978A 1978-10-09 1978-10-09 Extract of bacterial cells, its preparation, and anti- tumor agent containing the same Granted JPS5550895A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12435978A JPS5550895A (en) 1978-10-09 1978-10-09 Extract of bacterial cells, its preparation, and anti- tumor agent containing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12435978A JPS5550895A (en) 1978-10-09 1978-10-09 Extract of bacterial cells, its preparation, and anti- tumor agent containing the same

Publications (2)

Publication Number Publication Date
JPS5550895A true JPS5550895A (en) 1980-04-14
JPS6326117B2 JPS6326117B2 (en) 1988-05-27

Family

ID=14883439

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12435978A Granted JPS5550895A (en) 1978-10-09 1978-10-09 Extract of bacterial cells, its preparation, and anti- tumor agent containing the same

Country Status (1)

Country Link
JP (1) JPS5550895A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130245116A1 (en) * 2011-09-16 2013-09-19 U.S. Department Of Veterans Affairs Compositions and methods for the disruption of mycobacterium biofilm

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS504228A (en) * 1972-05-19 1975-01-17

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS504228A (en) * 1972-05-19 1975-01-17

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130245116A1 (en) * 2011-09-16 2013-09-19 U.S. Department Of Veterans Affairs Compositions and methods for the disruption of mycobacterium biofilm

Also Published As

Publication number Publication date
JPS6326117B2 (en) 1988-05-27

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