JPS5550895A - Extract of bacterial cells, its preparation, and anti- tumor agent containing the same - Google Patents
Extract of bacterial cells, its preparation, and anti- tumor agent containing the sameInfo
- Publication number
- JPS5550895A JPS5550895A JP12435978A JP12435978A JPS5550895A JP S5550895 A JPS5550895 A JP S5550895A JP 12435978 A JP12435978 A JP 12435978A JP 12435978 A JP12435978 A JP 12435978A JP S5550895 A JPS5550895 A JP S5550895A
- Authority
- JP
- Japan
- Prior art keywords
- extract
- cells
- removing treatment
- bacterial cells
- delayed allergy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
PURPOSE: To prepare a novel extract of bacterial cells, having anti-tumor activity, by disintegrating the cells of facteria having delayed allergy activating capability, extracting the disintegrated cells, and purifying the extract.
CONSTITUTION: An extract of bacterial cells, useful as an anti-tumor agent, containing ≤3% of lipids, ≤1% of nucleic acids, and ≤65% of glucides, and having a delayed allergy activating capability per unit weight of the protein of more than 4 times as high as the purified tuberculin, is prepared by the following steps; (1) mixing the cells of bacteria having delayed allergy activating capability, e.g. Mycobacterium tuberculosis, Mycobacterium smegmatis, etc. with a proper buffer solution, (2) disintegrating the cells with a disintegrator, (3) filtering or centrifuging the dispersion of the disintegrated cells to obtain an extract, (4) subjecting the extract to the nucleic acid-removing treatment and low-activity protein-removing treatment, (5) if necessary, subjecting the extract further to the lipid-removing treatment and/ or the glucide-removing treatment, and finally (6) fractionating the purified product with an ion exchange meterial.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12435978A JPS5550895A (en) | 1978-10-09 | 1978-10-09 | Extract of bacterial cells, its preparation, and anti- tumor agent containing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12435978A JPS5550895A (en) | 1978-10-09 | 1978-10-09 | Extract of bacterial cells, its preparation, and anti- tumor agent containing the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5550895A true JPS5550895A (en) | 1980-04-14 |
JPS6326117B2 JPS6326117B2 (en) | 1988-05-27 |
Family
ID=14883439
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12435978A Granted JPS5550895A (en) | 1978-10-09 | 1978-10-09 | Extract of bacterial cells, its preparation, and anti- tumor agent containing the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5550895A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130245116A1 (en) * | 2011-09-16 | 2013-09-19 | U.S. Department Of Veterans Affairs | Compositions and methods for the disruption of mycobacterium biofilm |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS504228A (en) * | 1972-05-19 | 1975-01-17 |
-
1978
- 1978-10-09 JP JP12435978A patent/JPS5550895A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS504228A (en) * | 1972-05-19 | 1975-01-17 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20130245116A1 (en) * | 2011-09-16 | 2013-09-19 | U.S. Department Of Veterans Affairs | Compositions and methods for the disruption of mycobacterium biofilm |
Also Published As
Publication number | Publication date |
---|---|
JPS6326117B2 (en) | 1988-05-27 |
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