JPS5517468A - Estimation method of alpha-fat protein - Google Patents
Estimation method of alpha-fat proteinInfo
- Publication number
- JPS5517468A JPS5517468A JP9073178A JP9073178A JPS5517468A JP S5517468 A JPS5517468 A JP S5517468A JP 9073178 A JP9073178 A JP 9073178A JP 9073178 A JP9073178 A JP 9073178A JP S5517468 A JPS5517468 A JP S5517468A
- Authority
- JP
- Japan
- Prior art keywords
- afp
- enzyme
- film
- electrode
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To estimate AFP correctly by electrolytic method, by using enzyme mark AFP (α-fat protein) and using the enzyme detection electrode with fixed antibody AFP film.
CONSTITUTION: At the external surface of the enzyme transmission film 2 consisting teflon and polyethyrene, the fixed antibody AFP film 3 fixed in macromolecular matrix (e.g., cellulose derivative) for the antibody AFP is bonded; the enzyme detection electrode 1 is used. The electrode 1 is immersed in the inspected solution 7 adding a given amount of enzyme mark AFP in the vessel 6, and after the reaction of antigen antibody on the fixed antibody AFP film 3, the film 3 is cleaned with physiological salt water to remove free AFP. Further, the electrode 1 is immersed in the hydrogen peroxide solution 9 in the vessel 8, and the amount of enzyme produced with the enzyme operation of enzyme mark AFP bonded on the surface of the film 3 is electrolytically measured at the electrode 1, and by comparing it with the amount estimation line made in advance through the use of standard AFP and the enzyme mark AFP, the amount of AFP in the inspected liquid can be known.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9073178A JPS5517468A (en) | 1978-07-25 | 1978-07-25 | Estimation method of alpha-fat protein |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9073178A JPS5517468A (en) | 1978-07-25 | 1978-07-25 | Estimation method of alpha-fat protein |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5517468A true JPS5517468A (en) | 1980-02-06 |
JPS617257B2 JPS617257B2 (en) | 1986-03-05 |
Family
ID=14006698
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9073178A Granted JPS5517468A (en) | 1978-07-25 | 1978-07-25 | Estimation method of alpha-fat protein |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5517468A (en) |
-
1978
- 1978-07-25 JP JP9073178A patent/JPS5517468A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS617257B2 (en) | 1986-03-05 |
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