JPS5517468A - Estimation method of alpha-fat protein - Google Patents

Estimation method of alpha-fat protein

Info

Publication number
JPS5517468A
JPS5517468A JP9073178A JP9073178A JPS5517468A JP S5517468 A JPS5517468 A JP S5517468A JP 9073178 A JP9073178 A JP 9073178A JP 9073178 A JP9073178 A JP 9073178A JP S5517468 A JPS5517468 A JP S5517468A
Authority
JP
Japan
Prior art keywords
afp
enzyme
film
electrode
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9073178A
Other languages
Japanese (ja)
Other versions
JPS617257B2 (en
Inventor
Shuichi Suzuki
Masuo Aizawa
Isao Ishiguro
Rikio Shinohara
Yoichi Nagamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Chemiphar Co Ltd
Original Assignee
Nippon Chemiphar Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Chemiphar Co Ltd filed Critical Nippon Chemiphar Co Ltd
Priority to JP9073178A priority Critical patent/JPS5517468A/en
Publication of JPS5517468A publication Critical patent/JPS5517468A/en
Publication of JPS617257B2 publication Critical patent/JPS617257B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To estimate AFP correctly by electrolytic method, by using enzyme mark AFP (α-fat protein) and using the enzyme detection electrode with fixed antibody AFP film.
CONSTITUTION: At the external surface of the enzyme transmission film 2 consisting teflon and polyethyrene, the fixed antibody AFP film 3 fixed in macromolecular matrix (e.g., cellulose derivative) for the antibody AFP is bonded; the enzyme detection electrode 1 is used. The electrode 1 is immersed in the inspected solution 7 adding a given amount of enzyme mark AFP in the vessel 6, and after the reaction of antigen antibody on the fixed antibody AFP film 3, the film 3 is cleaned with physiological salt water to remove free AFP. Further, the electrode 1 is immersed in the hydrogen peroxide solution 9 in the vessel 8, and the amount of enzyme produced with the enzyme operation of enzyme mark AFP bonded on the surface of the film 3 is electrolytically measured at the electrode 1, and by comparing it with the amount estimation line made in advance through the use of standard AFP and the enzyme mark AFP, the amount of AFP in the inspected liquid can be known.
COPYRIGHT: (C)1980,JPO&Japio
JP9073178A 1978-07-25 1978-07-25 Estimation method of alpha-fat protein Granted JPS5517468A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9073178A JPS5517468A (en) 1978-07-25 1978-07-25 Estimation method of alpha-fat protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9073178A JPS5517468A (en) 1978-07-25 1978-07-25 Estimation method of alpha-fat protein

Publications (2)

Publication Number Publication Date
JPS5517468A true JPS5517468A (en) 1980-02-06
JPS617257B2 JPS617257B2 (en) 1986-03-05

Family

ID=14006698

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9073178A Granted JPS5517468A (en) 1978-07-25 1978-07-25 Estimation method of alpha-fat protein

Country Status (1)

Country Link
JP (1) JPS5517468A (en)

Also Published As

Publication number Publication date
JPS617257B2 (en) 1986-03-05

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