JPS55139395A - Separation of unstable protein in stabilized form - Google Patents
Separation of unstable protein in stabilized formInfo
- Publication number
- JPS55139395A JPS55139395A JP4820779A JP4820779A JPS55139395A JP S55139395 A JPS55139395 A JP S55139395A JP 4820779 A JP4820779 A JP 4820779A JP 4820779 A JP4820779 A JP 4820779A JP S55139395 A JPS55139395 A JP S55139395A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- tubes
- aqueous glycerol
- solution
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: After aqueous glycerol is previously put in the bottom of the centrifugal tubes, a solution of human living protein containing a denaturant is placed in the tubes and subjected to centrifugation, thus collecting unstable protein in the aqueous glycerol without insolubilization by denaturation.
CONSTITUTION: A protein denaturant such as acid or ethanol is added to a solution of human living protein. In the meantime, an aqueous glycerol of 10W19vol% concentration is placed in the bottom of the centrifugal tubes previously and then the protein solution is placed thereon in double layer. Then, these tubes is subjected to centrifugation, e.g., under 3,000rpm for 15min to collect the unstable protein susceptible to insolubilization in the aqueous glycerol.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4820779A JPS55139395A (en) | 1979-04-19 | 1979-04-19 | Separation of unstable protein in stabilized form |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4820779A JPS55139395A (en) | 1979-04-19 | 1979-04-19 | Separation of unstable protein in stabilized form |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS55139395A true JPS55139395A (en) | 1980-10-31 |
Family
ID=12796939
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4820779A Pending JPS55139395A (en) | 1979-04-19 | 1979-04-19 | Separation of unstable protein in stabilized form |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55139395A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63125980U (en) * | 1987-02-12 | 1988-08-17 |
-
1979
- 1979-04-19 JP JP4820779A patent/JPS55139395A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63125980U (en) * | 1987-02-12 | 1988-08-17 | ||
JP2545217Y2 (en) * | 1987-02-12 | 1997-08-25 | 松下電器産業株式会社 | Washing machine valve operating device |
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