JPS55120788A - Adenosine monophosphate deaminase and its preparation - Google Patents

Adenosine monophosphate deaminase and its preparation

Info

Publication number
JPS55120788A
JPS55120788A JP2923979A JP2923979A JPS55120788A JP S55120788 A JPS55120788 A JP S55120788A JP 2923979 A JP2923979 A JP 2923979A JP 2923979 A JP2923979 A JP 2923979A JP S55120788 A JPS55120788 A JP S55120788A
Authority
JP
Japan
Prior art keywords
adenosine monophosphate
deaminase
adenosine
monophosphate deaminase
monophosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2923979A
Other languages
Japanese (ja)
Other versions
JPS609792B2 (en
Inventor
Keiko Murakami
Masataka Yoshino
Keizo Tsushima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP2923979A priority Critical patent/JPS609792B2/en
Publication of JPS55120788A publication Critical patent/JPS55120788A/en
Publication of JPS609792B2 publication Critical patent/JPS609792B2/en
Expired legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare adenosine monophosphate deaminase by culturing fungi belonging to Saccharomyces genus, etc.
CONSTITUTION: Fungi belonging to Saccharomyces, Hansenula, Kloechera, Candida, Torulopsis, or Endomycopsis genus, and capable of producing adenosine monophosphate deaminase, are inoculated in a nutrient medium, and cultured at 25W 35°C for 8W48hrs, and the accumulated adenosine monophosphate deaminase is separated from the medium. The adenosine monophosphate deaminase has a molecular weight of 280,000W320,000 by the gel-filtration and about 80,000 by SDS- electrophoresis, the optimum pH range, the stable pH range, the optimum temperature range, and the stable temperature range in a phosphate buffer solution of 7.5W7.8, 7W8, about 35°C, and lower than 40°C, respectively, and a Km value to adenosine-monophosphate of 2×10-6 M, and is capable of hydrolyzing adenosine monophosphate to inosine-monophosphate and ammonia.
COPYRIGHT: (C)1980,JPO&Japio
JP2923979A 1979-03-12 1979-03-12 Adenosine monophosphate deaminase Expired JPS609792B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2923979A JPS609792B2 (en) 1979-03-12 1979-03-12 Adenosine monophosphate deaminase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2923979A JPS609792B2 (en) 1979-03-12 1979-03-12 Adenosine monophosphate deaminase

Publications (2)

Publication Number Publication Date
JPS55120788A true JPS55120788A (en) 1980-09-17
JPS609792B2 JPS609792B2 (en) 1985-03-13

Family

ID=12270679

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2923979A Expired JPS609792B2 (en) 1979-03-12 1979-03-12 Adenosine monophosphate deaminase

Country Status (1)

Country Link
JP (1) JPS609792B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0716812A1 (en) * 1994-12-16 1996-06-19 Societe Des Produits Nestle S.A. Process for preparing a flavouring agent
WO2005105991A1 (en) * 2004-04-28 2005-11-10 Amano Enzyme Inc. Amp deaminase originating in streptomyces and utilization thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0716812A1 (en) * 1994-12-16 1996-06-19 Societe Des Produits Nestle S.A. Process for preparing a flavouring agent
WO2005105991A1 (en) * 2004-04-28 2005-11-10 Amano Enzyme Inc. Amp deaminase originating in streptomyces and utilization thereof
US7709240B2 (en) 2004-04-28 2010-05-04 Amano Enzyme Inc. AMP deaminase originating streptomyces and utilization thereof

Also Published As

Publication number Publication date
JPS609792B2 (en) 1985-03-13

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