JPS55111798A - Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof - Google Patents

Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof

Info

Publication number
JPS55111798A
JPS55111798A JP1908779A JP1908779A JPS55111798A JP S55111798 A JPS55111798 A JP S55111798A JP 1908779 A JP1908779 A JP 1908779A JP 1908779 A JP1908779 A JP 1908779A JP S55111798 A JPS55111798 A JP S55111798A
Authority
JP
Japan
Prior art keywords
enzyme
oxobarbituric
acid derivative
determination
labelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1908779A
Other languages
Japanese (ja)
Inventor
Yukio Kuroiwa
Kimiko Aoki
Namika Terasawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP1908779A priority Critical patent/JPS55111798A/en
Publication of JPS55111798A publication Critical patent/JPS55111798A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine with high sensitivity oxobarbituric acid derivative, which is a substance to be tested, from a trace amount of a sample by enzyme immunoassay method employing alkaliphosphatase as labelled enzyme.
CONSTITUTION: A sample contg. oxobarbituric acid derivative and an enzyme- labelled phenobarbital of the formula (wherein ALP is alkaliphosphatase residue; n is an integer of 1W20) are competitively reacted with anti-oxobarbiturate antibody. After the addition of buffer solution to the reaction mixt., ALP-labelled phenobarbital combined with insoluble antibody and uncombined ALP-labelled phenobarbital are separated by means of centrifugal separation or the like. After the separation, enzyme activity of one of them is measured by a conventional method such as Bessey-Lowry method to determine the amount of oxobarbituric acid derivative in the sample.
COPYRIGHT: (C)1980,JPO&Japio
JP1908779A 1979-02-22 1979-02-22 Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof Pending JPS55111798A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1908779A JPS55111798A (en) 1979-02-22 1979-02-22 Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1908779A JPS55111798A (en) 1979-02-22 1979-02-22 Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof

Publications (1)

Publication Number Publication Date
JPS55111798A true JPS55111798A (en) 1980-08-28

Family

ID=11989664

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1908779A Pending JPS55111798A (en) 1979-02-22 1979-02-22 Enzyme-labelled compound for determination of oxobarbituric acid derivative and method of determination thereof

Country Status (1)

Country Link
JP (1) JPS55111798A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62165151A (en) * 1986-01-13 1987-07-21 イ−ストマン　コダツク　カンパニ− Mark hydantoin joining body and analysis element thereof andusage in immunity analysis
JPH0671236A (en) * 1992-08-26 1994-03-15 Japan Field Kk Ultrasonic cleaning device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62165151A (en) * 1986-01-13 1987-07-21 イ−ストマン　コダツク　カンパニ− Mark hydantoin joining body and analysis element thereof andusage in immunity analysis
JPH0535989B2 (en) * 1986-01-13 1993-05-27 Eastman Kodak Co
JPH0671236A (en) * 1992-08-26 1994-03-15 Japan Field Kk Ultrasonic cleaning device

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