JPS5498306A - Preparation of human blood-plasma cholinesterase - Google Patents

Preparation of human blood-plasma cholinesterase

Info

Publication number
JPS5498306A
JPS5498306A JP530278A JP530278A JPS5498306A JP S5498306 A JPS5498306 A JP S5498306A JP 530278 A JP530278 A JP 530278A JP 530278 A JP530278 A JP 530278A JP S5498306 A JPS5498306 A JP S5498306A
Authority
JP
Japan
Prior art keywords
cholinesterase
plasma
precipitate
human blood
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP530278A
Other languages
Japanese (ja)
Other versions
JPS5811929B2 (en
Inventor
Kazumi Fukuyama
Yatsuhiro Kamimura
Satoru Funakoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP53005302A priority Critical patent/JPS5811929B2/en
Publication of JPS5498306A publication Critical patent/JPS5498306A/en
Publication of JPS5811929B2 publication Critical patent/JPS5811929B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

PURPOSE: To prepare high purity cholinesterase in high recovery, from human blood-plasma or a cholinesterase-containing fraction originated therefrom.
CONSTITUTION: (1) Human blood-plasma or a fraction of blood-plasma protein containing cholinesterase is fractionated in an aqueous medium containing 0.5W5% of proteins at pH3W7 with polyethylene glycol, and the precipitate is separated from the solution; (2) the precipitate is subjected to ion exchange chromatograpy in an aqueous medium at pH4W6; and (3) the eluted liquid is fractionated at a protein content of 0.01W5% and pH4W10 using ammonium sulfate and sodium sulfate, and the precipitate is separated from the solution. The steps (1) to (3) can be carried out in arbitrary order. The aqueous solution of cholinesterase thus obtained may be converted to a stable solid esterase preparation by adding a neutral amino acid, sugar, etc. as a stabilizing agent, followed by freeze-drying.
COPYRIGHT: (C)1979,JPO&Japio
JP53005302A 1978-01-23 1978-01-23 Process for producing human plasma cholinesterase Expired JPS5811929B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP53005302A JPS5811929B2 (en) 1978-01-23 1978-01-23 Process for producing human plasma cholinesterase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP53005302A JPS5811929B2 (en) 1978-01-23 1978-01-23 Process for producing human plasma cholinesterase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP57119162A Division JPS5822445B2 (en) 1982-07-08 1982-07-08 Method for producing stable solid human plasma cholinesterase preparation

Publications (2)

Publication Number Publication Date
JPS5498306A true JPS5498306A (en) 1979-08-03
JPS5811929B2 JPS5811929B2 (en) 1983-03-05

Family

ID=11607451

Family Applications (1)

Application Number Title Priority Date Filing Date
JP53005302A Expired JPS5811929B2 (en) 1978-01-23 1978-01-23 Process for producing human plasma cholinesterase

Country Status (1)

Country Link
JP (1) JPS5811929B2 (en)

Also Published As

Publication number Publication date
JPS5811929B2 (en) 1983-03-05

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