JPS5450394A - Fluorescence detector for liquid chromatography - Google Patents

Fluorescence detector for liquid chromatography

Info

Publication number
JPS5450394A
JPS5450394A JP11643377A JP11643377A JPS5450394A JP S5450394 A JPS5450394 A JP S5450394A JP 11643377 A JP11643377 A JP 11643377A JP 11643377 A JP11643377 A JP 11643377A JP S5450394 A JPS5450394 A JP S5450394A
Authority
JP
Japan
Prior art keywords
light
excited
lens system
condensing
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP11643377A
Other languages
Japanese (ja)
Inventor
Eiji Okada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP11643377A priority Critical patent/JPS5450394A/en
Publication of JPS5450394A publication Critical patent/JPS5450394A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors

Abstract

PURPOSE:To enhance the effective intensity of an excited light and the S/N sensitivity and to improve the metering efficiency by returning the excited light having passed through a detecting cell into a sample with the use of a concave reflecting mirror so that the fluorescent light irradiated may be reflected to a condensing lens by means of a condensing concave reflecting mirror. CONSTITUTION:A sample liquid 0 flowing through a detecting cell 1 is irradiated with an excited light, which is condensed by a condensing lens system 2, so that the energy condition of a substance the density of which is to be detected may be excited. The fluorescent light intrinsic to the substance, which is irradiated when transition takes place from the excited condition to a base condition, is condensed by a condensing lens system 3 so as to meter the density of the substance. A concave reflecting mirror 4 is arranged to face the condensing lens system 2 of the irradiating light so that the excited light having passed through the cell 1 may be reflected at 4 again into the cell 1. By means of a condensing reflecting mirror 5 which is arranged to face the condensing lens system 3, the fluorescent light intrinsic to the substance in the sample is reflected to the lens system 3. Thus, it is possible to increase the intensity of the excited light and the condensing efficiency of the fluorescent light.
JP11643377A 1977-09-28 1977-09-28 Fluorescence detector for liquid chromatography Pending JPS5450394A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11643377A JPS5450394A (en) 1977-09-28 1977-09-28 Fluorescence detector for liquid chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11643377A JPS5450394A (en) 1977-09-28 1977-09-28 Fluorescence detector for liquid chromatography

Publications (1)

Publication Number Publication Date
JPS5450394A true JPS5450394A (en) 1979-04-20

Family

ID=14686972

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11643377A Pending JPS5450394A (en) 1977-09-28 1977-09-28 Fluorescence detector for liquid chromatography

Country Status (1)

Country Link
JP (1) JPS5450394A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2561777A1 (en) * 1984-03-20 1985-09-27 Commissariat Energie Atomique Device for analysing <129>I2, particularly in gaseous effluent from nuclear fuel reprocessing plants
JP2008180567A (en) * 2007-01-24 2008-08-07 Shimadzu Corp Fluorometric spectrophotometer
JP2010249575A (en) * 2009-04-13 2010-11-04 Shimadzu Corp Spectrofluorophotometer

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2561777A1 (en) * 1984-03-20 1985-09-27 Commissariat Energie Atomique Device for analysing <129>I2, particularly in gaseous effluent from nuclear fuel reprocessing plants
JP2008180567A (en) * 2007-01-24 2008-08-07 Shimadzu Corp Fluorometric spectrophotometer
JP2010249575A (en) * 2009-04-13 2010-11-04 Shimadzu Corp Spectrofluorophotometer

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