JPH1198971A - Caries resisting agent and food and beverage containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a caries resisting agent obtained easily and at a low cost from an agricultural waste material, suppressing the generation of glucan from sucrose by a streptococcus in a mouth cavity, and useful for a food and a beverage by using a fraction extracted from a bagasse with an alkaline agent as an active ingredient. SOLUTION: This caries resisting agent uses a fraction obtained by extracting 1 pt.wt. solid portion of a bagasse such as a sugar cane bagasse produced as a byproduct in the production of a raw sugar from the sugar cane with 1-50 pt.wt. 0.05-2.0 N sodium hydroxide or potassium hydroxide aqueous solution, as an active ingredient. Further, it is preferable to blend 0.1-50 pt.wt. caries resisting agent with 100 pt.wt. caries forming component for preparing a food and beverage capable of suppressing the caries forming property.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-cariogenic agent which can be applied to the field of foods, in particular, the food, and is obtained by alkali extraction from bagasse, and a food and drink containing the same.

[0002]

2. Description of the Related Art Caries are caused by the action of glucosyltransferase, which is an insoluble glucan-forming enzyme of Streptococcus mutans , which is an oral streptococcus. It is caused by the formation of plaque by adhering to the skin. Within the plaque, Streptococcus mutans and various lactic acid bacteria grow and produce lactic acid from various carbon sources as well as sucrose. Lactic acid, whose diffusion into saliva is suppressed by plaque, is locally concentrated and demineralizes enamel, causing caries. As described above, the development of dental caries is a so-called cariogenic component, which is a component that is a raw material for plaque formation such as Streptococcus mutans, which is an oral streptococcus, and sucrose, and a place of activity. The three elements of the tooth are involved. Therefore, if one of these elements is removed, caries can be prevented, and various measures have conventionally been taken. For example, it is performed by means of the dental field, such as brushing teeth, eliminating Streptococcus mutans by using antibiotics, and strengthening the tooth material (enamel). However, it is very difficult to prevent caries directly in daily eating habits, and it is impossible to eliminate sucrose intake in today's eating habits.

[0003] Therefore, instead of sucrose, which causes plaque formation, it is not utilized by Streptococcus mutans and does not become a substrate for enzymatic action, that is, a sugar substitute that does not produce adherent insoluble glucan, ie, low cariogenicity Sweeteners have been developed and their use has been attempted,
Its sweetness and taste are inferior to sucrose, and have disadvantages such as higher cost.

[0004] Also, unlike low-cariogenic sweeteners, anti-cariogenic agents have been developed which are formulated into sucrose to prevent plaque formation from sucrose. For example, it is disclosed that a green tea extract containing a main component of polyphenol (JP-A-3-77817) and an oolong tea extract (JP-A-4-178320) suppress the production of insoluble glucan. However, the color,
There is a problem in taste and cost, and its use is limited.

As an anti-cariogenic agent extracted from a natural material with alkali, for example, Japanese Patent Application Laid-Open No. 6-279250 discloses an anti-cariogenic agent containing a hemicellulose B fraction derived from grains as an active ingredient. A method for producing the hemicellulose B fraction was reported in 1975 by Southgate D.T. ("The Chemistry of Dietary Fibe").
r "in" Fiber in Human Nutrition ", Spiller, GA
And Amen, RJ, p. 31, Plenum Press, New York
(1996)). The hemicellulose B fraction contains rice bran, cereal bran,
It is a water-soluble fraction mainly composed of arabinoxylan extracted from the residue obtained by removing lipids, starches, proteins, minerals, and the like from bran and hulls. Therefore,
This hemicellulose B fraction is distinctly different from the components extracted by direct alkali treatment of bagasse as in the present invention.

[0006]

SUMMARY OF THE INVENTION An object of the present invention is to obtain an inexpensive and easily effective anti-cariogenic agent from bagasse, which is originally an agricultural waste having little utility value.

[0007]

Means for Solving the Problems The present inventors examined whether or not bagasse, which is an agricultural waste, has an anti-cariogenic effect by using various extraction solvents for the extract. As a result, it has been found that by extracting from bagasse using an alkaline agent, an effective anti-carious action can be obtained from the extracted component, and the present invention has been completed.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION

As the bagasse used in the present invention, sugar cane bagasse produced as a by-product during production of raw sugar from sugar cane can be used.

The alkaline agent used in the alkaline extraction of the anti-cariogenic component from bagasse can be an alkaline solution, for example, an aqueous solution of sodium hydroxide and potassium hydroxide. The concentration of the alkaline solution is preferably 0.05 to 2.0N. The amount of the alkaline solution can be 1 to 50 parts by weight based on 1 part by weight of bagasse solids.

The temperature for the extraction may be from room temperature to 100 ° C., preferably 30 to 70 ° C. Extraction time is 1
A range of ~ 48 hours is preferred. At this time, it is also effective to appropriately stir. After completion of the extraction, the extract containing the active ingredient and the residue can be separated by centrifugation or filtration. After the extract is neutralized and desalted, it is concentrated,
Further, by adding a known excipient, a liquid, paste, powder or granular anti-cariogenic agent can be obtained.

As shown in Comparative Example 1 below, the hemicellulose B fraction from bagasse has no anti-caries effect.
Therefore, the anti-caries agent obtained from the bagasse of the present invention by alkali extraction is a substance different from the hemicellulose B fraction.

[0013] The anti-cariogenic agent obtained in the present invention can be used as a food or drink by using sucrose, which is a cariogenic component, as a part of a raw material, such as various beverages and confectionery. . The amount of the anti-caries agent of the present invention that exerts an anti-caries effect is 0.1 to 50 parts by weight, preferably 0.5 to 10 parts by weight, based on 100 parts by weight of the cariogenic component (e.g., sucrose). .

[0014]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the present invention is limited thereto. Example 1 Extraction of anti-cariogenic component, preparation of enzyme, anti-carious action
Measurement (1) Extraction of anti-cariogenic components from bagasse Take 10 g of dried bagasse from Okinawa in an Erlenmeyer flask, add 200 ml of 0-1.0 N sodium hydroxide solution or potassium hydroxide solution, and at 200 rpm at 30-70 ° C. Extracted for 6-24 hours with rotary shaking. The residue was removed by suction filtration, and the filtrate was neutralized with hydrochloric acid. The extract was obtained by removing the insoluble matter by centrifugation.

(2) Preparation of insoluble glucan-forming enzyme Streptococcus sobrinus strain 6715 (serotype g)
After culturing in 10 ml of a heart infusion (BHI) medium, 1 liter of the medium was inoculated and cultivated at 37 ° C. for 18 hours with nitrogen gas replacement. Centrifuge the obtained culture solution (7,500 rpm, 2
0 min) to obtain a culture supernatant. To this supernatant, 50% saturated ammonium sulfate was added for sedimentation. This was collected by centrifugation, dissolved in 25 ml of 50 mM phosphate buffer (pH 6.8), placed in a cellulose dialysis membrane (molecular weight cut off 10,000), and added to 2 liters of the same buffer (repeated several times). Dialyzed for hours. This was centrifuged to remove insolubles, and this was used as an insoluble glucan-forming enzyme.

(3) Measurement of anti-cariogenic action As a method of measuring the anti-cariogenic action, the effect of inhibiting the formation of insoluble glucan was measured. That is, 1% (w / v) sucrose, 0.0
5% (w / v) sodium azide, 0.1% (w / v) dextran T-10
(Pharmacia), 2.0 ml of 50 mM phosphate buffer (pH 6.8) containing each bagasse extract and insoluble glucan-forming enzyme
Was incubated at 37 ° C. for 18 hours. After the reaction, the mixture was stirred well with a mixer, and the turbidity at a wavelength of 550 nm was measured. The amount of enzyme used was the amount of enzyme in the reaction system whose turbidity when no extract was added was closest to 1.0. Usually, 0.1 ml of a 5-fold diluted enzyme solution was used. For the bagasse extract used, 0.2 ml of each extract (1/10 the amount of the reaction solution) was used. In the measurement of the inhibitory effect, it was previously confirmed that there was no influence of the salt contained in the extract. The inhibitory effect was defined as the turbidity without addition as a control, and the decrease in turbidity when the bagasse extract was added was expressed as a% inhibition rate.

(4) Results Influence of type and concentration of alkaline agent The results are shown in FIG. Both fractions extracted with potassium hydroxide and sodium hydroxide for 24 hours showed an effect of inhibiting the formation of insoluble glucan. Among them, when the concentration of sodium hydroxide was 0.1 N, the highest inhibitory effect was exhibited (inhibition rate 51%).

Effect of Extraction Temperature The results for sodium hydroxide are shown in FIG. 30 ℃, 50
The inhibitory effect was observed at both 70 ° C and 70 ° C, and the highest inhibitory effect was shown at 30 ° C.

Influence of extraction time The results for sodium hydroxide are shown in FIG. From 6 hours
The inhibitory effect was observed after 24 hours of extraction, and the highest inhibitory effect was obtained when the extraction was performed at a sodium hydroxide concentration of 0.2 N for 6 hours (inhibition rate 60%).

Example 2 Relationship between Concentration of Extract and Inhibitory Effect (1) Extraction of Anticariogenic Components from Bagasse To 100 g of the same bagasse used in Experimental Example 1, 2 liters of 0.1N sodium hydroxide solution was added. Extracted with stirring at 24 ° C. for 24 hours. The residue was removed by suction filtration, and the filtrate was neutralized with hydrochloric acid. Centrifuge to remove insolubles, put supernatant into cellulose dialysis membrane (molecular weight cut off 8,000), dialyze against 20 liters of tap water (change several times) overnight, and dialyze against 20 liters of demineralized water And desalted. After centrifugation, this was filtered through a membrane filter (3.0 μm) to remove insolubles,
The extract was concentrated to dryness to obtain 5.3 g of an extract.

(2) Measurement of Inhibitory Effect 0-200 μg of the extract was added as a bagasse extract to the reaction system of Example 1, (3), and the measurement was carried out by the method described in Example 1, (3).

(3) Constituents of the extract Table 1 shows the component analysis values in the extract obtained in (1). The sugar content is determined by the phenol-sulfuric acid method.
It was determined by analysis by HPLC. The protein content was measured by the Kjeldahl method.

Table 1 Dry weight% total sugar 83 (glucose) (8) (arabinose) (21) (xylose) (54) protein 11

(4) Results The results of the relationship between the extract concentration and the inhibitory effect are shown in FIG. As described above, the inhibitory effect increased as the concentration of the extract was increased. When the extract was added at a concentration of 60 μg / ml, the inhibition rate was about 50%.

Example 3 Example of application to hard candy In 1 liter of a 60% (w / v) sucrose solution, test (1)
Dissolve 3.0 g of bagasse extract obtained in (0.5% with respect to sucrose), heat and concentrate under reduced pressure until the water content becomes 2% or less, mix 10 g of citric acid and a small amount of lemon flavor and coloring agent The mixture was molded according to a conventional method to obtain a hard candy. This product was favorable without being particularly affected in both texture and sweetness as compared with the hard candy without the bagasse extract.

Example 4 Application to Chewing Gum 200 g of gum base was heated to a softening degree, and 600 g of sucrose powder and 3.0 g of bagasse extract obtained in (1) of Example 2 (0.5% based on sucrose) were added. Was further added, and a small amount of peppermint flavor and coloring agent were further mixed, kneaded with a roll according to a conventional method, and molded to obtain a chewing gum. This product is not particularly affected in both texture and sweetness compared to chewing gum without bagasse extract,
It was good.

Comparative Example 1 Hemicellulose B from bagasse
Minute of the anti-caries action (1) extracted from the bagasse of hemicellulose B fraction "dietary fiber" Japan Dietetic Association, ed., The first publication (1982) of "of dietary fiber chemistry", according to the method described in 17 to 21 pages, the following The hemicellulose B fraction was extracted from bagasse as described above. That is, 300 ml of benzene-ethanol (2: 1, v / v) was added to 10 g of the same bagasse used in Experimental Example 1, and stirred at room temperature overnight to extract lipids. The defatted bagasse collected by filtration with a Buchner funnel was washed again with the same reagent, filtered,
It was dried at 80 ° C. for 4 hours (dry weight 9.0 g). Suspend the dried defatted bagasse in 350 ml of demineralized water and maintain it at 75 ° C with acetic acid (0.
(42 ml) and sodium chlorite (5.3 g) were gradually added for delignification. After completion of delignification by adding the same amount of reagent three times every hour, the suspension is cooled to room temperature, filtered, washed three times with the same amount of demineralized water, and three times with the same amount of ethanol. After washing and drying, 7.5 g of holocellulose was obtained.

7.5 g of holocellulose was suspended in 250 ml of 1.0 N sodium hydroxide solution, and stirred at room temperature for 6 hours to extract hemicellulose. The filtered residue was further extracted similarly,
The residue was washed twice with the same amount of demineralized water. The extract and the washings were combined, neutralized with acetic acid, and concentrated under reduced pressure to 1/10 volume. After centrifugation to remove the precipitate of the concentrated solution (hemicellulose A fraction), the remaining supernatant was dialyzed overnight against 2 liters of tap water (change several times), and then 2 liters of demineralized water (change several times) Was dialyzed overnight. A three-fold amount of ethanol was added thereto, and the mixture was allowed to stand. The resulting precipitate (hemicellulose B fraction) was collected by centrifugation. This was washed and dried with 200 ml of 75% (w / v) ethanol and then with 200 ml of ethanol to obtain 3.0 g.

(2) Measurement of Inhibitory Effect of Insoluble Glucan Formation The hemicellulose B fraction obtained in (1) was adjusted to a final concentration of 100 μg / ml, and the inhibitory effect of insoluble glucan formation was obtained in the same manner as in Example 1, (3). Was examined. As a result, the inhibition rate was 0%, and no inhibitory effect was observed.

[0030]

According to the present invention, an inexpensive and easily effective anti-cariogenic agent can be provided by using an alkaline agent from bagasse, which is originally an agricultural waste having little utility value. In addition, foods and drinks that suppress cariogenicity can be provided by containing the anti-cariogenic agent.

[Brief description of the drawings]

FIG. 1 is a diagram showing the influence of the type and concentration of an alkaline agent.

FIG. 2 is a diagram showing the effect of extraction temperature.

FIG. 3 is a diagram showing the effect of extraction time.

FIG. 4 is a graph showing the relationship between the concentration of an extract and the inhibitory effect.

Claims (6)

[Claims] 1. An anti-cariogenic agent comprising a fraction extracted from bagasse with an alkaline agent as an active ingredient. 2. The anti-caries agent according to claim 1, wherein the alkaline agent is an aqueous solution of sodium hydroxide or potassium hydroxide. 3. The anti-caries agent according to claim 2, wherein the alkaline agent is an aqueous solution of 0.05 to 2.0 N sodium hydroxide or potassium hydroxide. 4. The anti-caries agent according to claim 2, wherein the alkali agent is 1 to 50 parts by weight based on 1 part by weight of bagasse solids in a 0.05 to 2.0 N aqueous solution of sodium hydroxide or potassium hydroxide. 5. A food or beverage which suppresses cariogenicity, which is characterized by comprising the anti-cariogenic agent according to any one of claims 1 to 4. 6. The amount of the anti-cariogenic agent according to claim 5 is:
A food or drink that suppresses cariogenicity, which is 0.1 to 50 parts by weight based on 100 parts by weight of a cariogenic component.