JP2004123604A - Prophylactic for tooth disease and method for producing food containing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a prophylactic for tooth diseases, especially a prophylactic for tooth diseases not deteriorating flavor, qualities, or the like, of a food product even if added to the food product. <P>SOLUTION: The prophylactic for tooth diseases contains 1,5-D-anhydrofructose. The method for producing the food product effective for preventing tooth diseases comprises adding 1,5-D-anhydrofructose to the food product. <P>COPYRIGHT: (C)2004,JPO

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a tooth disease preventive agent and a method for producing a food product that is effective in preventing tooth disease.
[0002]
[Prior art]
It is known that caries is caused by cariogenic bacteria, Streptococcus mutans, producing glucosyltransferase (GTase) and using sucrose as a substrate to form an insoluble, sticky polysaccharide glucan. In other words, this polysaccharide glucan adheres to the tooth surface together with the cariogenic bacteria to form plaque, and in the plaque, the tooth surface is affected by the action of organic acids produced by various bacteria including the carious bacteria. Occurs due to a decrease in the pH of the enamel and demineralization of the enamel. Therefore, in order to prevent caries, methods such as reducing sucrose, which is easily assimilated by cariogenic bacteria, substituting saccharides with a low carbohydrate, or compounding a substance having an anticariogenic action, etc. Can be considered. Substances having an anti-antibiotic effect include polyphenols and the like derived from natural products, but have a specific bitter taste, and there is a problem that the flavor of foods and the like is impaired depending on the amount added. In addition, a method of disinfecting and bacteriostatic dental caries is also effective, but among the disinfecting and bacteriostatic agents, it is a chemical substance. There was a problem that there was something.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide a tooth disease preventive agent, particularly a tooth disease preventive agent which does not deteriorate the flavor or quality of food even when added to food.
[0004]
Another object of the present invention is to provide a method for producing a food containing the prophylactic agent and effective for preventing dental diseases.
[0005]
Still other objects and advantages of the present invention will become apparent from the following description.
[0006]
[Means for Solving the Problems]
According to the present invention, the above-mentioned objects and advantages of the present invention are firstly achieved by a tooth disease preventive agent comprising 1,5-D-anhydrofructose.
[0007]
Further, according to the present invention, the above-mentioned object and advantages of the present invention are, secondly, adding 1,5-D-anhydrofructose to food, characterized in that a food effective for preventing dental diseases is provided. Achieved by manufacturing methods.
[0008]
Preferred Embodiment of the Invention
According to the study of the present inventors, 1,5-D-anhydrofructose (hereinafter, referred to as AF) has an antibacterial activity that is effective for preventing and improving bad breath, caries, and periodontal disease caused by oral bacteria. It was revealed to have. That is, the present inventors have found that AF has an effect of inhibiting the growth of Streptococcus mutans, which is a resident bacterium in the oral cavity, and an effect of inhibiting Gtase activity. Alternatively, it has been shown that it inhibits polysaccharide synthesis and eventually suppresses plaque formation to prevent tooth decay.
[0009]
AF used in the present invention can be obtained by reacting lyase extracted from red alga Ogonori on starch according to a method known per se. The present inventors have purified α-1,4-glucan lyase from the red alga Ogonori and made this oxygen act on starch to synthesize 1,5-D-anhydrofructose. This 1,5-D-anhydrofructose was purified and used as 1,5-D-anhydrofructose having a purity of 99% or more. AF can be used after heat treatment. The heat treatment conditions are preferably 50 to 150 ° C. for 1 second to 100 hours. For example, treatment at 50 ° C. under normal pressure for 10 minutes to 100 hours or treatment at 95 ° C. for 1 minute to 10 hours, or treatment under high pressure at 120 ° C. for 10 seconds to 2 hours or 130 to 150 ° C. for 1 second to 30 minutes Can be performed.
[0010]
The agent for preventing dental diseases containing AF of the present invention can be used by directly adding to oral agents such as oral fresheners, dentifrices, troches, mouthwashes, or foods and drinks.
[0011]
Foods include, for example, chewing gum, chocolate, ice cream, caramel, candy, cookies, coffee jelly, custard cream, Japanese and Western confectionery such as mizuyokan, castella, pudding, bean jam, jams, breads, noodles, rice , Coffee drinks, fruit drinks, beverages such as carbonated drinks, cheeses, fermented milk, milk and dairy products such as lactic acid bacteria drinks, fish meat paste products, processed meat products, sauces, seasonings such as dressings and the like. it can.
[0012]
The compounding ratio of AF to the food varies in suitable range depending on the type of food, storage method, storage period, storage environment, and the like. For example, 0.001 to 10 parts by weight, preferably 0.01 to 10 parts by weight, per 100 parts by weight of food. It can be 5 parts by weight.
[0013]
Hereinafter, the present invention will be described in detail with reference to examples. The present invention is not limited to these.
[0014]
【Example】
Example 1 Growth Inhibition of Streptococcus mutans by AF Streptococcus mutans (IFO13955) was cultured in 500 ml of LB medium at 37 ° C., the absorbance at 600 nm was measured every 4 hours, and a growth curve was taken. The composition of the LB medium is 10 g / l Polypeptide, 5 g / l yeast extract, 10 g / l NaCl, pH 7.0.
[0015]
Test plot 1. 1. LB medium test group to which AF was added to 1% LB medium without addition of AF
FIG. The results are shown in FIG. Test plot 1. Indicates test group 2. The growth of the bacterium is delayed by about 6 hours, which indicates that Streptococcus mutans is inhibited by AF.
[0017]
Example 2 Inhibition of GTase Activity by AF Streptococcus mutans (IFO13955) was cultured at 37 ° C in 500 ml of LB medium, and cells were removed by centrifugation to obtain a crude enzyme solution. From this crude enzyme solution, a 50% saturated ammonium sulfate fraction was recovered, dialyzed against a pH 6.8 phosphate buffer, and used as GTase. The enzymatic reaction was performed at 37 ° C. for 1 hour, and the enzymatic activity was evaluated by quantifying the fructose released into the reaction solution.
[0018]
Test plot 1. Sucrose 10%, Clinical dextran 0.5%
Test plot 2. Sucrose 10%, Clinical dextran 0.5% + AF 0.5%
Test plot 3. Sucrose 10%, Clinical dextran 0.5% + AF 0.25%
Test plot 4. Sucrose 10%, Clinical dextran 0.5% + AF 0.1%
[0019]
As a result, the test plot 1. Is 100, test group 2. Is 36.7, test plot 3. Is 69.9, test plot 4. Was 85.6, indicating that AF significantly inhibited GTase activity.
[Brief description of the drawings]
FIG. 1 shows the relationship between culture time and turbidity showing the inhibition of growth of Streptococcus mutans by AF in Example 1.
[Explanation of symbols]
1 Test Zone 1
2 test area 2

Claims (2)

An agent for preventing tooth disease, comprising 1,5-D-anhydrofructose. A method for producing a food which is effective in preventing dental diseases, comprising adding 1,5-D-anhydrofructose to the food.

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